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The map label for this gene is wcaJ [H]

Identifier: 94314754

GI number: 94314754

Start: 22727

End: 24133

Strand: Direct

Name: wcaJ [H]

Synonym: Rmet_5835

Alternate gene names: 94314754

Gene position: 22727-24133 (Clockwise)

Preceding gene: 291481468

Following gene: 94314755

Centisome position: 0.88

GC content: 64.96

Gene sequence:

>1407_bases
ATGGCGTCGGAATGGAATGTTGCCGCCGAGCGGCACTCTGCACTTCACTATCTCTATCGACTCGGTGATGCGGTACTGAT
CGCGGTATGCGGCATCGCCATGGGCGCGCTGTATTTCCATGACGGCATCAGGAGCGCGGCACCCGTCAACGGGTTCCTGA
CGATGCTGTGCGCGCTCGGCGCGCTGCTGATCTTTCCTGCCTTCGGCATCTACGAATCATGGCGCGGCCGCAGTCGCGCC
GTGCTGGGGTTGCGCATTGCCGCCGCATGGACCTTGGTGTTCATCTCCGGCCTGCTCGGTGCGTTCCTGATCCACCAGAT
CGGGGCGGTCTCACGCGTCTGGTCGATGGGCTGGTTCATTGGCACGGGCGTGATGCTGGCGCTGTCGCGGGTCGTGATGT
TCCGGATGCTGGCCAACGTGCGCCAGCAGGGCATCAACGCCAAGCGCGTGCTGATCTTCGGCTACGGCCCGCTGGGCCGC
GAAATGTACGATCGCGTGCAACACTTCCGCGATGCCGGCTATCGCGTCGTGGGCGTGTATGACGAGGAGTCCGACGCGAT
TCCGTACGACGTGACCCCGCTGCGCCAGATCGAGGAAGTCAGCGGCTTCGTGCGCGACCATGGCGTGCGCGAGATCTGGC
TGACGCTGCCGATGGCCGCTTGCCGCGATCTGTCCGGCGTGGTGCGCCAGTTCCGCAATGAGATGATCGACATCCGCTGG
GTGCCCGACGTGTCGTCCGTCGAGCTGCTGGGCCACCGCTTCAGCGAGTTCATGGGTCTGCCCGTCATCGACCTGAACAG
TCCGCCCGTCTCGGGCATCACGGGCGTGGTCAAGGCCAGCTTCGATCGCGTGTTCTCGCTCGGTGTGCTGGTCTGCCTGA
GCCCGTTGCTGTTGCTGATAGCCGCGATGGTCAAGCTGTCGTCGCCCGGTCCCGTGCTGTTCCGGCAGCAGCGCCTGGGC
ATCGACGGCCAGCCTTTCAACGTGTACAAGTTCCGCACCATGCGCCAGCACCAGGACGGCGGTGGCGTGACCCAGGCCGT
GCGCGGTGATCCGCGCGTGACCCGCGTCGGCGCGTTCCTGCGCCGGACCAGCCTCGACGAGTTGCCGCAGTTCATCAACG
TGCTGCGCGGCGAGATGTCAGTGGTCGGCCCGCGTCCGCACGCGATGGAGCATAACGAGCTCTACAAGGAACTGATCGAC
CGCTACATGCTGCGCCACCGCGTGAAGCCCGGCATCACCGGCTGGGCCCAGATCAACGGTCTGCGTGGCCAGACGGACAC
GGTCGAGAAGATGCGCAAGCGCATCGAGTTCGACATCTACTACATCCAGCACTGGTCGTTCCAGCTCGACCTTCGGATCA
TCCTGCGCACCGCGCTGCATGGCTGGACCGGCACCACGGCTTACTGA

Upstream 100 bases:

>100_bases
CGCAGGCGCTCGAGGGGTTTGTGCGCCTGCCAACAGACGACGCGCCATACGTGCGCAACCATGACTGCACTTGGTTTCCC
GCGAGGGCTTTGGGGCGGTC

Downstream 100 bases:

>100_bases
ACCAACAATAAAGAGACCCACTGAGGGGTAGACGCCGCCGCGCGCTCGGTTTGGTGCGAAATCGCACCGAAACGACCACG
CAAAACCCATGGAATGGAGC

Product: UDP-sugar lipid carrier transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MASEWNVAAERHSALHYLYRLGDAVLIAVCGIAMGALYFHDGIRSAAPVNGFLTMLCALGALLIFPAFGIYESWRGRSRA
VLGLRIAAAWTLVFISGLLGAFLIHQIGAVSRVWSMGWFIGTGVMLALSRVVMFRMLANVRQQGINAKRVLIFGYGPLGR
EMYDRVQHFRDAGYRVVGVYDEESDAIPYDVTPLRQIEEVSGFVRDHGVREIWLTLPMAACRDLSGVVRQFRNEMIDIRW
VPDVSSVELLGHRFSEFMGLPVIDLNSPPVSGITGVVKASFDRVFSLGVLVCLSPLLLLIAAMVKLSSPGPVLFRQQRLG
IDGQPFNVYKFRTMRQHQDGGGVTQAVRGDPRVTRVGAFLRRTSLDELPQFINVLRGEMSVVGPRPHAMEHNELYKELID
RYMLRHRVKPGITGWAQINGLRGQTDTVEKMRKRIEFDIYYIQHWSFQLDLRIILRTALHGWTGTTAY

Sequences:

>Translated_468_residues
MASEWNVAAERHSALHYLYRLGDAVLIAVCGIAMGALYFHDGIRSAAPVNGFLTMLCALGALLIFPAFGIYESWRGRSRA
VLGLRIAAAWTLVFISGLLGAFLIHQIGAVSRVWSMGWFIGTGVMLALSRVVMFRMLANVRQQGINAKRVLIFGYGPLGR
EMYDRVQHFRDAGYRVVGVYDEESDAIPYDVTPLRQIEEVSGFVRDHGVREIWLTLPMAACRDLSGVVRQFRNEMIDIRW
VPDVSSVELLGHRFSEFMGLPVIDLNSPPVSGITGVVKASFDRVFSLGVLVCLSPLLLLIAAMVKLSSPGPVLFRQQRLG
IDGQPFNVYKFRTMRQHQDGGGVTQAVRGDPRVTRVGAFLRRTSLDELPQFINVLRGEMSVVGPRPHAMEHNELYKELID
RYMLRHRVKPGITGWAQINGLRGQTDTVEKMRKRIEFDIYYIQHWSFQLDLRIILRTALHGWTGTTAY
>Mature_467_residues
ASEWNVAAERHSALHYLYRLGDAVLIAVCGIAMGALYFHDGIRSAAPVNGFLTMLCALGALLIFPAFGIYESWRGRSRAV
LGLRIAAAWTLVFISGLLGAFLIHQIGAVSRVWSMGWFIGTGVMLALSRVVMFRMLANVRQQGINAKRVLIFGYGPLGRE
MYDRVQHFRDAGYRVVGVYDEESDAIPYDVTPLRQIEEVSGFVRDHGVREIWLTLPMAACRDLSGVVRQFRNEMIDIRWV
PDVSSVELLGHRFSEFMGLPVIDLNSPPVSGITGVVKASFDRVFSLGVLVCLSPLLLLIAAMVKLSSPGPVLFRQQRLGI
DGQPFNVYKFRTMRQHQDGGGVTQAVRGDPRVTRVGAFLRRTSLDELPQFINVLRGEMSVVGPRPHAMEHNELYKELIDR
YMLRHRVKPGITGWAQINGLRGQTDTVEKMRKRIEFDIYYIQHWSFQLDLRIILRTALHGWTGTTAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=425, Percent_Identity=40.9411764705882, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 52649; Mature: 52518

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASEWNVAAERHSALHYLYRLGDAVLIAVCGIAMGALYFHDGIRSAAPVNGFLTMLCALG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ALLIFPAFGIYESWRGRSRAVLGLRIAAAWTLVFISGLLGAFLIHQIGAVSRVWSMGWFI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTGVMLALSRVVMFRMLANVRQQGINAKRVLIFGYGPLGREMYDRVQHFRDAGYRVVGVY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEE
DEESDAIPYDVTPLRQIEEVSGFVRDHGVREIWLTLPMAACRDLSGVVRQFRNEMIDIRW
ECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
VPDVSSVELLGHRFSEFMGLPVIDLNSPPVSGITGVVKASFDRVFSLGVLVCLSPLLLLI
CCCCHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAMVKLSSPGPVLFRQQRLGIDGQPFNVYKFRTMRQHQDGGGVTQAVRGDPRVTRVGAFL
HHHHHCCCCCCEEEEHHHCCCCCCCCCCEEHHHHHHCCCCCCCHHHHCCCCCHHHHHHHH
RRTSLDELPQFINVLRGEMSVVGPRPHAMEHNELYKELIDRYMLRHRVKPGITGWAQING
HHCCHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
LRGQTDTVEKMRKRIEFDIYYIQHWSFQLDLRIILRTALHGWTGTTAY
CCCCHHHHHHHHHHHHEEEEEEEEECEEHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ASEWNVAAERHSALHYLYRLGDAVLIAVCGIAMGALYFHDGIRSAAPVNGFLTMLCALG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ALLIFPAFGIYESWRGRSRAVLGLRIAAAWTLVFISGLLGAFLIHQIGAVSRVWSMGWFI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTGVMLALSRVVMFRMLANVRQQGINAKRVLIFGYGPLGREMYDRVQHFRDAGYRVVGVY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEE
DEESDAIPYDVTPLRQIEEVSGFVRDHGVREIWLTLPMAACRDLSGVVRQFRNEMIDIRW
ECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
VPDVSSVELLGHRFSEFMGLPVIDLNSPPVSGITGVVKASFDRVFSLGVLVCLSPLLLLI
CCCCHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAMVKLSSPGPVLFRQQRLGIDGQPFNVYKFRTMRQHQDGGGVTQAVRGDPRVTRVGAFL
HHHHHCCCCCCEEEEHHHCCCCCCCCCCEEHHHHHHCCCCCCCHHHHCCCCCHHHHHHHH
RRTSLDELPQFINVLRGEMSVVGPRPHAMEHNELYKELIDRYMLRHRVKPGITGWAQING
HHCCHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
LRGQTDTVEKMRKRIEFDIYYIQHWSFQLDLRIILRTALHGWTGTTAY
CCCCHHHHHHHHHHHHEEEEEEEEECEEHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]