Click here to switch to the map view.

The map label for this gene is cspA [H]

Identifier: 94314735

GI number: 94314735

Start: 2579327

End: 2579530

Strand: Direct

Name: cspA [H]

Synonym: Rmet_5816

Alternate gene names: 94314735

Gene position: 2579327-2579530 (Clockwise)

Preceding gene: 94314731

Following gene: NA

Centisome position: 99.97

GC content: 56.86

Gene sequence:

>204_bases
ATGCAAACCGGTATCGTAAAGTGGTTCAACGACGCCAAGGGCTTTGGCTTCATCAAGCCGGACGCTGGCGGTGACGATCT
GTTCGCTCACTTCTCGGAAGTCCGTGCCGACGGCTTCAAGTCGCTGCAGGAAAACCAACGCGTGTCGTTCGAAGTCAAGA
ACGGCCCGAAGGGTCTGCAAGCAGCGAACATCACCCCGCTGTAA

Upstream 100 bases:

>100_bases
CTTGGTACTTCGGTTGTCCATGGTGTCCTTTCCTTGAGTTCGCTGTACGGTGGTGTGTGCACCATCGGTTTCTTTTATAT
TTTTTTTGGAAAGCAATACC

Downstream 100 bases:

>100_bases
GCGTTGTGATTCCGAGTGCACCTTGACGGTGCGCTCGATCGCGAAGAACCCGCCTTTATGGCGGGTTTTTTTACGCCCAA
CGCTTTCATAGCGCCCCAAG

Product: major cold shock protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 67

Protein sequence:

>67_residues
MQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEVRADGFKSLQENQRVSFEVKNGPKGLQAANITPL

Sequences:

>Translated_67_residues
MQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEVRADGFKSLQENQRVSFEVKNGPKGLQAANITPL
>Mature_67_residues
MQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEVRADGFKSLQENQRVSFEVKNGPKGLQAANITPL

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=53.7313432835821, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1789979, Length=66, Percent_Identity=59.0909090909091, Blast_Score=85, Evalue=8e-19,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=57.8125, Blast_Score=83, Evalue=3e-18,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=53.8461538461538, Blast_Score=79, Evalue=4e-17,
Organism=Escherichia coli, GI2367114, Length=65, Percent_Identity=50.7692307692308, Blast_Score=79, Evalue=8e-17,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=53.125, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787839, Length=63, Percent_Identity=50.7936507936508, Blast_Score=73, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24658520, Length=65, Percent_Identity=41.5384615384615, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7342; Mature: 7342

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEVRADGFKSLQENQRVSFEVKNGPKGLQ
CCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
AANITPL
CCCCCCC
>Mature Secondary Structure
MQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEVRADGFKSLQENQRVSFEVKNGPKGLQ
CCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
AANITPL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]