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The map label for this gene is atoC [H]

Identifier: 94314732

GI number: 94314732

Start: 2575065

End: 2576567

Strand: Reverse

Name: atoC [H]

Synonym: Rmet_5813

Alternate gene names: 94314732

Gene position: 2576567-2575065 (Counterclockwise)

Preceding gene: 94314733

Following gene: 94314730

Centisome position: 99.86

GC content: 65.0

Gene sequence:

>1503_bases
GTGATTCCCACGTTGCGCGCGGCACCGACCGCGCAGCCAGCGGACCCCGAACGGGCCATGCGGCTCGCCGCCACACCTGC
GGGCTTGGGTTCCGCCACTAACAAGAAGTTCAACGTCCGGGAGAACGTCATGGACCGACTGTGCAAGGCGCCGACACGGA
CCGCACAGATCGTGCTCGTGTCGCGCTATGAAGATTTTGGATTTAACGCGAACCAGTTTGGCTCCGGATGGGAAATCCGC
CGGGTGCAGCAGGTTCGCGACCTCGAGCGCCTGGTGCGTGCGAGTTCGCCCATGGCTGGCGTGCTCGACCTGCAGACGCC
TTACACCGACGCCGAGCTGAGCCAGCTCGAACAGGCGCTACAGCACCTGCATGTGATGTGGGTCGCCATTACATCCACGG
CAATGCTGGAGGACGAGCGCATCCGTCGTCTGATCCGGGACTATTGCGTCGACTACGTGCGCACGCCATTCTCGTTCGAC
GAGTTGCTTTACACGCTCAAGCACGCGCATGGCATGGCATGTCTGCACGGGGCTCGCTCGCCCGAACTGGTGGCGCGCGG
GCCGATGATCGGAGAGTGCGCGCCCATGGGCAACATGTTCCGCTCGCTGGCGAAGGTCGCGCACAACGACGCGCCGGTGT
TCATCTCCGGCGAATCGGGCACGGGCAAGGAACTCGCAGCCCAGGCCATCCACGAAGCGTCGAGCCGCCGCAAGGGGCCG
TTCGTGGCGATCAACTGCGGCGCGATTCCATCGCACCTCGTGCAATCGGAACTGTTCGGCTACGAGAAAGGCGCGTTCAC
CGGCGCGAACCAGCGCAAGATCGGCTGGATCGAGCAGGCCCAGGGCGGCACGCTGTTCCTCGACGAGATTGGTGACCTGC
CGCTGGAAAGCCAGGTTGCCCTGCTGCGATTCCTGCAGCAAGGCATGATCACGCGCCTAGGCGGGCACCAGTCGATTCCG
CTCGACCTGCGCATCATCTCCGCCACGCACGTGGACCTGGTGGCGGCACAGAGCGACGGCCGGTTCCGCTCCGATCTGTT
CCACCGGCTCTGCGTGCTGACCCTGACCATTCCGCCGCTGCGCGAGCGTGGCGAGGACATCCTGCTGCTCGCGAATACGG
TGCTGGCCGAACACGGCCACGAGGCGCACCGGCGTATCCGTGGCTTCTCCACCTGCGCGACGCAGGCCATGATGCAGTAC
ATGTGGCCCGGAAATGTGCGCGAACTGATCAACCGCGTGCGTCGCGCCATCGTGATGACCGACAACCGGAAAATCACGGC
GGAGGATCTGCAACTGCACGGTGGGGTGGAGGTGCCACGCAAGACGCTGGATGCGATTCGCGAGGAGGCGGAACGCGAGG
CGATCCGCTCCGTCATGGCCAGCCATGGTTTTCACGTGGTGCCGGCGGCGCGCGAGCTCAACGTGTCGCGCGTGACGCTA
TACCGGTTGATGCACAAGCACAACATCCGCATCGACACGCAGCCCGGCGCGTCGGCGGAGTAG

Upstream 100 bases:

>100_bases
TCGAAAAGGACATGGTGCGCCGACGAATCCGCGACCAGCTGAGCGCAGGACGCGACCAGGTCAGCGTCGCGGAAGAATCC
CCACACAGTTTGTCCAGACC

Downstream 100 bases:

>100_bases
CGACTTCGATGCAGAAAGTTCTGAAACAGCACGTGCAGTAAGTGCCAAGGGAGACTTGGCGACGTCCGCTCGCCAAGTCT
CCTTTGTCCTGCCGCCCGGA

Product: sigma54 specific transcriptional regulator, Fis family

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MIPTLRAAPTAQPADPERAMRLAATPAGLGSATNKKFNVRENVMDRLCKAPTRTAQIVLVSRYEDFGFNANQFGSGWEIR
RVQQVRDLERLVRASSPMAGVLDLQTPYTDAELSQLEQALQHLHVMWVAITSTAMLEDERIRRLIRDYCVDYVRTPFSFD
ELLYTLKHAHGMACLHGARSPELVARGPMIGECAPMGNMFRSLAKVAHNDAPVFISGESGTGKELAAQAIHEASSRRKGP
FVAINCGAIPSHLVQSELFGYEKGAFTGANQRKIGWIEQAQGGTLFLDEIGDLPLESQVALLRFLQQGMITRLGGHQSIP
LDLRIISATHVDLVAAQSDGRFRSDLFHRLCVLTLTIPPLRERGEDILLLANTVLAEHGHEAHRRIRGFSTCATQAMMQY
MWPGNVRELINRVRRAIVMTDNRKITAEDLQLHGGVEVPRKTLDAIREEAEREAIRSVMASHGFHVVPAARELNVSRVTL
YRLMHKHNIRIDTQPGASAE

Sequences:

>Translated_500_residues
MIPTLRAAPTAQPADPERAMRLAATPAGLGSATNKKFNVRENVMDRLCKAPTRTAQIVLVSRYEDFGFNANQFGSGWEIR
RVQQVRDLERLVRASSPMAGVLDLQTPYTDAELSQLEQALQHLHVMWVAITSTAMLEDERIRRLIRDYCVDYVRTPFSFD
ELLYTLKHAHGMACLHGARSPELVARGPMIGECAPMGNMFRSLAKVAHNDAPVFISGESGTGKELAAQAIHEASSRRKGP
FVAINCGAIPSHLVQSELFGYEKGAFTGANQRKIGWIEQAQGGTLFLDEIGDLPLESQVALLRFLQQGMITRLGGHQSIP
LDLRIISATHVDLVAAQSDGRFRSDLFHRLCVLTLTIPPLRERGEDILLLANTVLAEHGHEAHRRIRGFSTCATQAMMQY
MWPGNVRELINRVRRAIVMTDNRKITAEDLQLHGGVEVPRKTLDAIREEAEREAIRSVMASHGFHVVPAARELNVSRVTL
YRLMHKHNIRIDTQPGASAE
>Mature_500_residues
MIPTLRAAPTAQPADPERAMRLAATPAGLGSATNKKFNVRENVMDRLCKAPTRTAQIVLVSRYEDFGFNANQFGSGWEIR
RVQQVRDLERLVRASSPMAGVLDLQTPYTDAELSQLEQALQHLHVMWVAITSTAMLEDERIRRLIRDYCVDYVRTPFSFD
ELLYTLKHAHGMACLHGARSPELVARGPMIGECAPMGNMFRSLAKVAHNDAPVFISGESGTGKELAAQAIHEASSRRKGP
FVAINCGAIPSHLVQSELFGYEKGAFTGANQRKIGWIEQAQGGTLFLDEIGDLPLESQVALLRFLQQGMITRLGGHQSIP
LDLRIISATHVDLVAAQSDGRFRSDLFHRLCVLTLTIPPLRERGEDILLLANTVLAEHGHEAHRRIRGFSTCATQAMMQY
MWPGNVRELINRVRRAIVMTDNRKITAEDLQLHGGVEVPRKTLDAIREEAEREAIRSVMASHGFHVVPAARELNVSRVTL
YRLMHKHNIRIDTQPGASAE

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=341, Percent_Identity=43.4017595307918, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790437, Length=336, Percent_Identity=41.6666666666667, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1788905, Length=308, Percent_Identity=42.8571428571429, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI1790299, Length=368, Percent_Identity=39.945652173913, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87082117, Length=274, Percent_Identity=47.8102189781022, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789233, Length=343, Percent_Identity=38.4839650145773, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1786524, Length=358, Percent_Identity=38.8268156424581, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87082152, Length=337, Percent_Identity=40.0593471810089, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1789087, Length=308, Percent_Identity=40.5844155844156, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=36.0501567398119, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1787583, Length=307, Percent_Identity=33.8762214983713, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI87081858, Length=292, Percent_Identity=33.5616438356164, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1789828, Length=241, Percent_Identity=34.4398340248963, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55634; Mature: 55634

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPTLRAAPTAQPADPERAMRLAATPAGLGSATNKKFNVRENVMDRLCKAPTRTAQIVLV
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
SRYEDFGFNANQFGSGWEIRRVQQVRDLERLVRASSPMAGVLDLQTPYTDAELSQLEQAL
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHH
QHLHVMWVAITSTAMLEDERIRRLIRDYCVDYVRTPFSFDELLYTLKHAHGMACLHGARS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCC
PELVARGPMIGECAPMGNMFRSLAKVAHNDAPVFISGESGTGKELAAQAIHEASSRRKGP
CCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
FVAINCGAIPSHLVQSELFGYEKGAFTGANQRKIGWIEQAQGGTLFLDEIGDLPLESQVA
EEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCEEEHHHHCCCCCHHHHH
LLRFLQQGMITRLGGHQSIPLDLRIISATHVDLVAAQSDGRFRSDLFHRLCVLTLTIPPL
HHHHHHHHHHHHCCCCCCCCEEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHCCCCH
RERGEDILLLANTVLAEHGHEAHRRIRGFSTCATQAMMQYMWPGNVRELINRVRRAIVMT
HHCCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEE
DNRKITAEDLQLHGGVEVPRKTLDAIREEAEREAIRSVMASHGFHVVPAARELNVSRVTL
CCCEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCHHHHHH
YRLMHKHNIRIDTQPGASAE
HHHHHHCCCEEECCCCCCCC
>Mature Secondary Structure
MIPTLRAAPTAQPADPERAMRLAATPAGLGSATNKKFNVRENVMDRLCKAPTRTAQIVLV
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
SRYEDFGFNANQFGSGWEIRRVQQVRDLERLVRASSPMAGVLDLQTPYTDAELSQLEQAL
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHH
QHLHVMWVAITSTAMLEDERIRRLIRDYCVDYVRTPFSFDELLYTLKHAHGMACLHGARS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCCC
PELVARGPMIGECAPMGNMFRSLAKVAHNDAPVFISGESGTGKELAAQAIHEASSRRKGP
CCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
FVAINCGAIPSHLVQSELFGYEKGAFTGANQRKIGWIEQAQGGTLFLDEIGDLPLESQVA
EEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCEEEHHHHCCCCCHHHHH
LLRFLQQGMITRLGGHQSIPLDLRIISATHVDLVAAQSDGRFRSDLFHRLCVLTLTIPPL
HHHHHHHHHHHHCCCCCCCCEEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHCCCCH
RERGEDILLLANTVLAEHGHEAHRRIRGFSTCATQAMMQYMWPGNVRELINRVRRAIVMT
HHCCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEE
DNRKITAEDLQLHGGVEVPRKTLDAIREEAEREAIRSVMASHGFHVVPAARELNVSRVTL
CCCEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCHHHHHH
YRLMHKHNIRIDTQPGASAE
HHHHHHCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]