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The map label for this gene is puuD [H]

Identifier: 94314679

GI number: 94314679

Start: 2521434

End: 2522237

Strand: Reverse

Name: puuD [H]

Synonym: Rmet_5760

Alternate gene names: 94314679

Gene position: 2522237-2521434 (Counterclockwise)

Preceding gene: 94314683

Following gene: 94314678

Centisome position: 97.76

GC content: 64.05

Gene sequence:

>804_bases
ATGTCATCCGCCCCAATTCAAAAGTCACTTCTTCCGGTCGTGGCGATCATCACGGATCGTATCGACCTCCACAGGCATCC
GGCTTACTCGGTTCTCGGTGGCTATGTGCGGGCTGTTAGCGAGATTGCCGGTGCGCAGCCGCTGCTGCTCCCGGCGTGCG
CCGACGCCATCGATGCCGACACGTTGATCGACACGCTGGACGGCATCGTCCTGACCGGCAGTCCGTCCAACATCGCGGCG
GAAAGGTATGGAGCCACGCCCCTGCCGGCAATGACATGCCAGGACCCCCACCGTGACGCCGCGGTTTTTGGTCTGCTGCC
AGCCCTGATCGATGCCGGGGTCCCAATACTGGGGATTTGCCGTGGCTTTCAGGAGTTGAACGTCTTGCATGGCGGCACGC
TCGAGCGCGCGGTGCACGCCCAGCCTGGCCGACTGGATCATCGGGAGGGCGACCACGACCGACCGATCCAGCAGTGGTAC
GACGATAGCCACGATATTCACATCGTGCCTGGCGGCTGGCTGGCAGGGATCGCCGGGCGTTCGGCCGCGGTCAATTCGCT
GCATCACCAGGGCGTCGAGCGGCTCGGTGCCGGACTGCGTATCGAAGCGACGGCGCCCGATGGACTCGTGGAGGCGTTCT
CGCTTGCCAACGCAGCGCAATTCACGCTTGCCGTCCAGTGGCATCCGGAGATGCGTATCGACGATTGCGGCCTGGCCAAA
GCGATCTTTTCCGAGTTTGGACGCGCATGTGGCGAACGTCGCGCCACTCGGACGCGCGCATCCAGACCGACATTCCCTGC
CTGA

Upstream 100 bases:

>100_bases
TTGCATAAATTGCATTCTAACCAGAATTTAGTTGCAATTTACACAAGTAGTGGATGTCTCCAAACTGCGGCCAGATCCAT
TCCGACTGCCACATCGTTCC

Downstream 100 bases:

>100_bases
GGTCATCGACATATGGAATCAGCGCTGCATCATCGAGGAGTCAAGGTGCAACCAGAAGTTCGTAACCTGTTCAGTCGAAC
CTACGCGGAGGCCCGGCAGA

Product: peptidase C26

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSSAPIQKSLLPVVAIITDRIDLHRHPAYSVLGGYVRAVSEIAGAQPLLLPACADAIDADTLIDTLDGIVLTGSPSNIAA
ERYGATPLPAMTCQDPHRDAAVFGLLPALIDAGVPILGICRGFQELNVLHGGTLERAVHAQPGRLDHREGDHDRPIQQWY
DDSHDIHIVPGGWLAGIAGRSAAVNSLHHQGVERLGAGLRIEATAPDGLVEAFSLANAAQFTLAVQWHPEMRIDDCGLAK
AIFSEFGRACGERRATRTRASRPTFPA

Sequences:

>Translated_267_residues
MSSAPIQKSLLPVVAIITDRIDLHRHPAYSVLGGYVRAVSEIAGAQPLLLPACADAIDADTLIDTLDGIVLTGSPSNIAA
ERYGATPLPAMTCQDPHRDAAVFGLLPALIDAGVPILGICRGFQELNVLHGGTLERAVHAQPGRLDHREGDHDRPIQQWY
DDSHDIHIVPGGWLAGIAGRSAAVNSLHHQGVERLGAGLRIEATAPDGLVEAFSLANAAQFTLAVQWHPEMRIDDCGLAK
AIFSEFGRACGERRATRTRASRPTFPA
>Mature_266_residues
SSAPIQKSLLPVVAIITDRIDLHRHPAYSVLGGYVRAVSEIAGAQPLLLPACADAIDADTLIDTLDGIVLTGSPSNIAAE
RYGATPLPAMTCQDPHRDAAVFGLLPALIDAGVPILGICRGFQELNVLHGGTLERAVHAQPGRLDHREGDHDRPIQQWYD
DSHDIHIVPGGWLAGIAGRSAAVNSLHHQGVERLGAGLRIEATAPDGLVEAFSLANAAQFTLAVQWHPEMRIDDCGLAKA
IFSEFGRACGERRATRTRASRPTFPA

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=251, Percent_Identity=38.2470119521912, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 28453; Mature: 28321

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAPIQKSLLPVVAIITDRIDLHRHPAYSVLGGYVRAVSEIAGAQPLLLPACADAIDAD
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
TLIDTLDGIVLTGSPSNIAAERYGATPLPAMTCQDPHRDAAVFGLLPALIDAGVPILGIC
HHHHHHCCEEEECCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHH
RGFQELNVLHGGTLERAVHAQPGRLDHREGDHDRPIQQWYDDSHDIHIVPGGWLAGIAGR
HHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEECCCHHHHHCCC
SAAVNSLHHQGVERLGAGLRIEATAPDGLVEAFSLANAAQFTLAVQWHPEMRIDDCGLAK
HHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHH
AIFSEFGRACGERRATRTRASRPTFPA
HHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SSAPIQKSLLPVVAIITDRIDLHRHPAYSVLGGYVRAVSEIAGAQPLLLPACADAIDAD
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
TLIDTLDGIVLTGSPSNIAAERYGATPLPAMTCQDPHRDAAVFGLLPALIDAGVPILGIC
HHHHHHCCEEEECCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHH
RGFQELNVLHGGTLERAVHAQPGRLDHREGDHDRPIQQWYDDSHDIHIVPGGWLAGIAGR
HHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEECCCHHHHHCCC
SAAVNSLHHQGVERLGAGLRIEATAPDGLVEAFSLANAAQFTLAVQWHPEMRIDDCGLAK
HHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHH
AIFSEFGRACGERRATRTRASRPTFPA
HHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]