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The map label for this gene is citA [H]

Identifier: 94314677

GI number: 94314677

Start: 2518931

End: 2520262

Strand: Reverse

Name: citA [H]

Synonym: Rmet_5758

Alternate gene names: 94314677

Gene position: 2520262-2518931 (Counterclockwise)

Preceding gene: 94314678

Following gene: 94314676

Centisome position: 97.68

GC content: 64.41

Gene sequence:

>1332_bases
ATGCGCGCCACCAGTCCGGCGTCTGCCCAGACGCTTGCGGTTCACACGGCGAGGGATGTCGGCGAGCCCGACGTTCGGCC
AGTTATTGGTCGGGCCGCGGTTGCCGCCGCCGTTGCAGGCAACGCCCTCGAGTTCTACGACTTCGTCATCTACGCTTACT
TTGCCGTCTACATCGGTCGCGCATTTTTTCCGGTCGGTGGCGAGTTCGGCAGCCTGCTGCTCGCGGCCGCCACCTTCGGA
GTGGGATTCTTCACTCGCCCACTCGGCGCCGTACTGATCGGCGCGCTGGCTGACAGGGCCGGTCGCAAGCCTGCAATGAT
CCTGACCGTGGCGTTGATCACCTTTGGCACGCTTGGCATGGCCGCCACGCCAAGCTATGCGTCTATCGGTATCGCCGCTC
CCATCATCGTGGTGGCTTGCCGTCTGCTACAGGGCCTTGCGCTCGGCGGAGAGGTGGGGCCAGCAACCGCGCTACTCGTC
GAGGCCGCACCGCCGCATCGCCGTGCACTTTACGCGAGTTGGCAATTGGCCAGCCAGGGTATTGCCGTGGCGGTTGGTGG
ACTGCTGGGCGTTGCGGTGTCGATGTTGCTTCCGGCGGAAGACCTGGCCGCCTGGGGGTGGCGTATCCCCTTCGTGATCA
GCATCGCGCTCGTGCCGGTCGCCCTGAAGATTCGCCGCAGCCTGCCCGAAACCTTCGAAGCCCGGACCGAGCGCAGCACC
GCCGAGGTGGTCGGTGGCCTGATGAAGTCGCACCGGCGCTATGTTGTTCTGGGTGTGCTGATGGTGCTGTCTGCCTCCGT
CGCGTCTCAGATTGGCAACTACATGACCACTTACGCGATCCAGACGTTGCACCTGCCCGCAACACTTGCGCAGACCAGTG
CGCTGGTTGGTGGCGTGATGACGTTCGCGTTCGGCCTGGTGGGAGGCTGGCTATGCGATTGGCGCGGCCGGAAGATTGTC
CTGATTGCGCCGCGCGTCGCGCTGATGCTGTTGATCGTGCCAATGTTCTGGTGGCTGGGCGCAATGCCCGGTGCCATCAG
CTTCCTGACCGTGACAGCGGTACTCGCAGCGCTCACCGCCATGAGTGCCGCGGCATCGATCGTCGTGATTCCCGAGTTGT
TGCCGGCTGGCTTTCGCAGTATCGGCGTCTCTCTGGTCTACGCGATCGGTACCACGCTATTCGGAGGGACCACGCAGTTC
GTGGTGACAGGCTTGCTGGCCTGGACGCACAACCCTAATGCGCCGGCCTGGTACCTCGCCGCCACCAGCATCGTCAGCCT
TGTGGCGATCTGGCTCCTGCCGGAAACGCGCGGCACCGACATCTCCGAATAG

Upstream 100 bases:

>100_bases
CAGGGCCGCGACGTGTTCCTGCAGACCCTGAATGGACTGCGGGAAGACTGATCGTCTGAGCAGGCCCTCAGCCCATTCAT
AACGATTCCAAGGAGACAAA

Downstream 100 bases:

>100_bases
CACACACCTGTTCGCCAGTGGGACGCAGTGCCGTCCCAGTGTTCCCAATCAGCAGTGCGAGCCAAAGCCGGGGTAATTCG
CTGGCGGGGGGCAATTGCGC

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MRATSPASAQTLAVHTARDVGEPDVRPVIGRAAVAAAVAGNALEFYDFVIYAYFAVYIGRAFFPVGGEFGSLLLAAATFG
VGFFTRPLGAVLIGALADRAGRKPAMILTVALITFGTLGMAATPSYASIGIAAPIIVVACRLLQGLALGGEVGPATALLV
EAAPPHRRALYASWQLASQGIAVAVGGLLGVAVSMLLPAEDLAAWGWRIPFVISIALVPVALKIRRSLPETFEARTERST
AEVVGGLMKSHRRYVVLGVLMVLSASVASQIGNYMTTYAIQTLHLPATLAQTSALVGGVMTFAFGLVGGWLCDWRGRKIV
LIAPRVALMLLIVPMFWWLGAMPGAISFLTVTAVLAALTAMSAAASIVVIPELLPAGFRSIGVSLVYAIGTTLFGGTTQF
VVTGLLAWTHNPNAPAWYLAATSIVSLVAIWLLPETRGTDISE

Sequences:

>Translated_443_residues
MRATSPASAQTLAVHTARDVGEPDVRPVIGRAAVAAAVAGNALEFYDFVIYAYFAVYIGRAFFPVGGEFGSLLLAAATFG
VGFFTRPLGAVLIGALADRAGRKPAMILTVALITFGTLGMAATPSYASIGIAAPIIVVACRLLQGLALGGEVGPATALLV
EAAPPHRRALYASWQLASQGIAVAVGGLLGVAVSMLLPAEDLAAWGWRIPFVISIALVPVALKIRRSLPETFEARTERST
AEVVGGLMKSHRRYVVLGVLMVLSASVASQIGNYMTTYAIQTLHLPATLAQTSALVGGVMTFAFGLVGGWLCDWRGRKIV
LIAPRVALMLLIVPMFWWLGAMPGAISFLTVTAVLAALTAMSAAASIVVIPELLPAGFRSIGVSLVYAIGTTLFGGTTQF
VVTGLLAWTHNPNAPAWYLAATSIVSLVAIWLLPETRGTDISE
>Mature_443_residues
MRATSPASAQTLAVHTARDVGEPDVRPVIGRAAVAAAVAGNALEFYDFVIYAYFAVYIGRAFFPVGGEFGSLLLAAATFG
VGFFTRPLGAVLIGALADRAGRKPAMILTVALITFGTLGMAATPSYASIGIAAPIIVVACRLLQGLALGGEVGPATALLV
EAAPPHRRALYASWQLASQGIAVAVGGLLGVAVSMLLPAEDLAAWGWRIPFVISIALVPVALKIRRSLPETFEARTERST
AEVVGGLMKSHRRYVVLGVLMVLSASVASQIGNYMTTYAIQTLHLPATLAQTSALVGGVMTFAFGLVGGWLCDWRGRKIV
LIAPRVALMLLIVPMFWWLGAMPGAISFLTVTAVLAALTAMSAAASIVVIPELLPAGFRSIGVSLVYAIGTTLFGGTTQF
VVTGLLAWTHNPNAPAWYLAATSIVSLVAIWLLPETRGTDISE

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=422, Percent_Identity=32.7014218009479, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1788292, Length=419, Percent_Identity=30.7875894988067, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1790550, Length=441, Percent_Identity=28.3446712018141, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789941, Length=436, Percent_Identity=32.5688073394495, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI87082231, Length=421, Percent_Identity=26.8408551068884, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082404, Length=375, Percent_Identity=24.5333333333333, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46229; Mature: 46229

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRATSPASAQTLAVHTARDVGEPDVRPVIGRAAVAAAVAGNALEFYDFVIYAYFAVYIGR
CCCCCCCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AFFPVGGEFGSLLLAAATFGVGFFTRPLGAVLIGALADRAGRKPAMILTVALITFGTLGM
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
AATPSYASIGIAAPIIVVACRLLQGLALGGEVGPATALLVEAAPPHRRALYASWQLASQG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCHHHHHHHHHHHHHCC
IAVAVGGLLGVAVSMLLPAEDLAAWGWRIPFVISIALVPVALKIRRSLPETFEARTERST
HHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHH
AEVVGGLMKSHRRYVVLGVLMVLSASVASQIGNYMTTYAIQTLHLPATLAQTSALVGGVM
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TFAFGLVGGWLCDWRGRKIVLIAPRVALMLLIVPMFWWLGAMPGAISFLTVTAVLAALTA
HHHHHHHHHHEECCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
MSAAASIVVIPELLPAGFRSIGVSLVYAIGTTLFGGTTQFVVTGLLAWTHNPNAPAWYLA
HHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHH
ATSIVSLVAIWLLPETRGTDISE
HHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRATSPASAQTLAVHTARDVGEPDVRPVIGRAAVAAAVAGNALEFYDFVIYAYFAVYIGR
CCCCCCCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AFFPVGGEFGSLLLAAATFGVGFFTRPLGAVLIGALADRAGRKPAMILTVALITFGTLGM
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
AATPSYASIGIAAPIIVVACRLLQGLALGGEVGPATALLVEAAPPHRRALYASWQLASQG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCHHHHHHHHHHHHHCC
IAVAVGGLLGVAVSMLLPAEDLAAWGWRIPFVISIALVPVALKIRRSLPETFEARTERST
HHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHH
AEVVGGLMKSHRRYVVLGVLMVLSASVASQIGNYMTTYAIQTLHLPATLAQTSALVGGVM
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TFAFGLVGGWLCDWRGRKIVLIAPRVALMLLIVPMFWWLGAMPGAISFLTVTAVLAALTA
HHHHHHHHHHEECCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
MSAAASIVVIPELLPAGFRSIGVSLVYAIGTTLFGGTTQFVVTGLLAWTHNPNAPAWYLA
HHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHH
ATSIVSLVAIWLLPETRGTDISE
HHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]