Click here to switch to the map view.

The map label for this gene is kdsA [H]

Identifier: 94314652

GI number: 94314652

Start: 2493269

End: 2494108

Strand: Direct

Name: kdsA [H]

Synonym: Rmet_5733

Alternate gene names: 94314652

Gene position: 2493269-2494108 (Clockwise)

Preceding gene: 291481603

Following gene: 94314653

Centisome position: 96.64

GC content: 59.52

Gene sequence:

>840_bases
ATGGCTACGATAAAAAACCTCTCCGTGCCTAACGACGCATCTCTTGTTCTCTTTGGCGGCATCAATGTGCTGGAATCCCG
CGACCTGGCAATGCAAGCCTGCGCCGCCTACGTAGAAATCACCACCCGCCTGGGGATTCCGTACGTCTTCAAGGCCAGCT
TTGATAAGGCGAACCGCTCATCGATTCACTCCTATCGTGGACCGGGTCTGGAGGAAGGGCTGCGCATTCTCCAGGACGTA
AAAGCCGCGTTCGATGTGCCCGTGCTTACAGATATCCATGAGCCCTGGCAAGCTGCCCCGGTCTCGGAAGTGGCTGACGT
ACTGCAGCTTCCCGCCTTCCTGGCGAGGCAGACAGATCTCGTTGTATCGCTAGCTCGCACGGGCCGGCCGATCAATATCA
AGAAGCCGCAGTTCATGAGCCCAACGCAGATTCAGCACATCGTCGAAAAATTCCGCGAGGCTGGCAACGAGAACCTTCTG
CTCTGTGATCGCGGTACATGCTTCGGCTACGACAACCTCGTGGTCGATATGCTTGGATTTAGCGTCATGCGCCGGGCGAC
GGGAGACCTGCCGATTGTCTTTGACGTGACGCACAGCCTGCAGCAACGAGACCCCGGCAACGCGGCTTCTGGCGGGCGTC
GGACTCAGGTGGTGGAACTCGCGCGGGCCGGCGTTGCCGTCGGCATTGCCGGCCTCTTTCTGGAAGCGCACCCCGAGCCG
GATCGAGCGCTATGCGATGGCCCGAGTGCACTGCCACTGGACCAACTTGAGCCCTTCCTCACCCAGGTAAAGATGCTCGA
CGATGTTGTGAAATCGCTGCCGGCAACGGAGCTCGCCTGA

Upstream 100 bases:

>100_bases
AAGACAGCCAATCACGATCCAGTAGATTCAATCCATTCATCTGGACAAGCCGACAACAGCCCCCACAGTTGACGGCTCGT
GGCTGTGCAACGAACTGCTG

Downstream 100 bases:

>100_bases
TGACACGGCAGGATGGCGAGAGCGTTGTCATCGTCATTCCCGCGCGCTACGGATCGACGCGCTTGCCTGGCAAGCCGTTG
CTGGATATTGCCGGCAAACC

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase 2; KDO-8-phosphate synthase 2; KDO 8-P synthase 2; KDOPS 2; Phospho-2-dehydro-3-deoxyoctonate aldolase 2 [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MATIKNLSVPNDASLVLFGGINVLESRDLAMQACAAYVEITTRLGIPYVFKASFDKANRSSIHSYRGPGLEEGLRILQDV
KAAFDVPVLTDIHEPWQAAPVSEVADVLQLPAFLARQTDLVVSLARTGRPINIKKPQFMSPTQIQHIVEKFREAGNENLL
LCDRGTCFGYDNLVVDMLGFSVMRRATGDLPIVFDVTHSLQQRDPGNAASGGRRTQVVELARAGVAVGIAGLFLEAHPEP
DRALCDGPSALPLDQLEPFLTQVKMLDDVVKSLPATELA

Sequences:

>Translated_279_residues
MATIKNLSVPNDASLVLFGGINVLESRDLAMQACAAYVEITTRLGIPYVFKASFDKANRSSIHSYRGPGLEEGLRILQDV
KAAFDVPVLTDIHEPWQAAPVSEVADVLQLPAFLARQTDLVVSLARTGRPINIKKPQFMSPTQIQHIVEKFREAGNENLL
LCDRGTCFGYDNLVVDMLGFSVMRRATGDLPIVFDVTHSLQQRDPGNAASGGRRTQVVELARAGVAVGIAGLFLEAHPEP
DRALCDGPSALPLDQLEPFLTQVKMLDDVVKSLPATELA
>Mature_278_residues
ATIKNLSVPNDASLVLFGGINVLESRDLAMQACAAYVEITTRLGIPYVFKASFDKANRSSIHSYRGPGLEEGLRILQDVK
AAFDVPVLTDIHEPWQAAPVSEVADVLQLPAFLARQTDLVVSLARTGRPINIKKPQFMSPTQIQHIVEKFREAGNENLLL
CDRGTCFGYDNLVVDMLGFSVMRRATGDLPIVFDVTHSLQQRDPGNAASGGRRTQVVELARAGVAVGIAGLFLEAHPEPD
RALCDGPSALPLDQLEPFLTQVKMLDDVVKSLPATELA

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family [H]

Homologues:

Organism=Escherichia coli, GI1787466, Length=273, Percent_Identity=67.3992673992674, Blast_Score=404, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.55 [H]

Molecular weight: Translated: 30301; Mature: 30170

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKNLSVPNDASLVLFGGINVLESRDLAMQACAAYVEITTRLGIPYVFKASFDKANRS
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHH
SIHSYRGPGLEEGLRILQDVKAAFDVPVLTDIHEPWQAAPVSEVADVLQLPAFLARQTDL
HHHHHCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VVSLARTGRPINIKKPQFMSPTQIQHIVEKFREAGNENLLLCDRGTCFGYDNLVVDMLGF
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHH
SVMRRATGDLPIVFDVTHSLQQRDPGNAASGGRRTQVVELARAGVAVGIAGLFLEAHPEP
HHHHHHCCCCCEEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCC
DRALCDGPSALPLDQLEPFLTQVKMLDDVVKSLPATELA
CCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ATIKNLSVPNDASLVLFGGINVLESRDLAMQACAAYVEITTRLGIPYVFKASFDKANRS
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHH
SIHSYRGPGLEEGLRILQDVKAAFDVPVLTDIHEPWQAAPVSEVADVLQLPAFLARQTDL
HHHHHCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VVSLARTGRPINIKKPQFMSPTQIQHIVEKFREAGNENLLLCDRGTCFGYDNLVVDMLGF
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHH
SVMRRATGDLPIVFDVTHSLQQRDPGNAASGGRRTQVVELARAGVAVGIAGLFLEAHPEP
HHHHHHCCCCCEEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCC
DRALCDGPSALPLDQLEPFLTQVKMLDDVVKSLPATELA
CCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]