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The map label for this gene is cat [H]

Identifier: 94314645

GI number: 94314645

Start: 2485038

End: 2485763

Strand: Reverse

Name: cat [H]

Synonym: Rmet_5726

Alternate gene names: 94314645

Gene position: 2485763-2485038 (Counterclockwise)

Preceding gene: 94314646

Following gene: 94314644

Centisome position: 96.34

GC content: 57.99

Gene sequence:

>726_bases
ATGCGCGAGTGGCTCCTATCCGCCATTTACAAAGGAAGAATCATGCCGACAGAGAACGAGGCTGAGCGACAGTCCGCCGA
TATCCGCAAGCGCTGCGCCGAACTCGGCGGCCGCATCGCTCCTACAGCACAAGTCAGCAGTGCGGTGAGCTTCGACGAAG
CGATTGATATTGGTGACGGCGCTGTGGCCTACGCGCCGGCATTTATTGGCGGGTTCACCTACGTCAATGTAGGTGCTGTG
GTCTTTCCCCACGTCCGCATCGGCAAATTCTGCTCGATCGGGCGTAACGTGCAGATCGGGGCGGCACGTCACCCCGTGGA
ATTCCTGAGTTCCCACCCATTCCAATTCTCCCCAGCGCTCTTCAATCGCGTGCCGGGCTATCCTGCCTTGCAGACAGAGA
AGTGGCGCTTTCACGCGCCAACTACCGTTGGCAACGACGTTTGGCTTGGAACGAACTGCGTAATCGTGGCTGGCGTCAAG
ATTGGGGATGGGGCGGTTGTGGGCGCTGGCGCTGTGGTGACGAAGGATGTCGCCCCTTACGAAATCGTGGGAGGAGTTCC
AGCGCGGCGCATCCGACTTCGCTTCGATGAAGACAAAGTGTCGCGCCTGCTCAAGACACGCTGGTGGGATCTTCCCCTTG
ATTCATTGAAAGATATTCCGTTCGGCAATATCGATCAGGCCATTGCATCCATTGAAGCCATCCGAAGCAGCAAGGAAAGC
GCGTGA

Upstream 100 bases:

>100_bases
AGGAAGTTTCGCCAGAACCCTTATGCCTTCTTCCGCGACGCAAACTCGCCACTACTGCGTTCGGTGGCCCGGCTGATTGC
ACATTGAATGAGGGGTAGCC

Downstream 100 bases:

>100_bases
AACACTCGCTATCAAGAGTACTCGGTTCCATTCTTGCAGCCACCGTCTTTCCGTGCGGTCTGGCTTTTGCTGACGACTGT
ACGCTGCCGGCGCTGGACAA

Product: Chloramphenicol acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: Virginiamycin acetyltransferase D; Vat(D) [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MREWLLSAIYKGRIMPTENEAERQSADIRKRCAELGGRIAPTAQVSSAVSFDEAIDIGDGAVAYAPAFIGGFTYVNVGAV
VFPHVRIGKFCSIGRNVQIGAARHPVEFLSSHPFQFSPALFNRVPGYPALQTEKWRFHAPTTVGNDVWLGTNCVIVAGVK
IGDGAVVGAGAVVTKDVAPYEIVGGVPARRIRLRFDEDKVSRLLKTRWWDLPLDSLKDIPFGNIDQAIASIEAIRSSKES
A

Sequences:

>Translated_241_residues
MREWLLSAIYKGRIMPTENEAERQSADIRKRCAELGGRIAPTAQVSSAVSFDEAIDIGDGAVAYAPAFIGGFTYVNVGAV
VFPHVRIGKFCSIGRNVQIGAARHPVEFLSSHPFQFSPALFNRVPGYPALQTEKWRFHAPTTVGNDVWLGTNCVIVAGVK
IGDGAVVGAGAVVTKDVAPYEIVGGVPARRIRLRFDEDKVSRLLKTRWWDLPLDSLKDIPFGNIDQAIASIEAIRSSKES
A
>Mature_241_residues
MREWLLSAIYKGRIMPTENEAERQSADIRKRCAELGGRIAPTAQVSSAVSFDEAIDIGDGAVAYAPAFIGGFTYVNVGAV
VFPHVRIGKFCSIGRNVQIGAARHPVEFLSSHPFQFSPALFNRVPGYPALQTEKWRFHAPTTVGNDVWLGTNCVIVAGVK
IGDGAVVGAGAVVTKDVAPYEIVGGVPARRIRLRFDEDKVSRLLKTRWWDLPLDSLKDIPFGNIDQAIASIEAIRSSKES
A

Specific function: Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=180, Percent_Identity=31.6666666666667, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786664, Length=127, Percent_Identity=35.4330708661417, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 26179; Mature: 26179

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREWLLSAIYKGRIMPTENEAERQSADIRKRCAELGGRIAPTAQVSSAVSFDEAIDIGDG
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHEECCCC
AVAYAPAFIGGFTYVNVGAVVFPHVRIGKFCSIGRNVQIGAARHPVEFLSSHPFQFSPAL
CHHHHHHHHHCHHHEECCHHEECCCHHHHHHHCCCCEEECCCCCCHHHHHCCCCEECHHH
FNRVPGYPALQTEKWRFHAPTTVGNDVWLGTNCVIVAGVKIGDGAVVGAGAVVTKDVAPY
HHCCCCCCCCCCCCEEEECCCCCCCCEEECCCEEEEEEEEECCCCEECCCCEEECCCCCH
EIVGGVPARRIRLRFDEDKVSRLLKTRWWDLPLDSLKDIPFGNIDQAIASIEAIRSSKES
HHCCCCCCEEEEEEECHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCC
A
C
>Mature Secondary Structure
MREWLLSAIYKGRIMPTENEAERQSADIRKRCAELGGRIAPTAQVSSAVSFDEAIDIGDG
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHEECCCC
AVAYAPAFIGGFTYVNVGAVVFPHVRIGKFCSIGRNVQIGAARHPVEFLSSHPFQFSPAL
CHHHHHHHHHCHHHEECCHHEECCCHHHHHHHCCCCEEECCCCCCHHHHHCCCCEECHHH
FNRVPGYPALQTEKWRFHAPTTVGNDVWLGTNCVIVAGVKIGDGAVVGAGAVVTKDVAPY
HHCCCCCCCCCCCCEEEECCCCCCCCEEECCCEEEEEEEEECCCCEECCCCEEECCCCCH
EIVGGVPARRIRLRFDEDKVSRLLKTRWWDLPLDSLKDIPFGNIDQAIASIEAIRSSKES
HHCCCCCCEEEEEEECHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8257133 [H]