Click here to switch to the map view.

The map label for this gene is pstS [H]

Identifier: 94314582

GI number: 94314582

Start: 2422290

End: 2423357

Strand: Reverse

Name: pstS [H]

Synonym: Rmet_5663

Alternate gene names: 94314582

Gene position: 2423357-2422290 (Counterclockwise)

Preceding gene: 291481598

Following gene: 94314580

Centisome position: 93.93

GC content: 64.61

Gene sequence:

>1068_bases
ATGCTCAAGCGCACCGCGTTGGCCATTCTTGGCTCAGCGCTCATCATGTCCACTGCCGTAGCCGCTGATATCCAGGGCGC
GGGTTCGACGGCGGCCGCGCCGGTCTATCGCGTCTGGTCGACCGGCTATGCCGCGGCCACCGGCACCCGGCTCAATTACG
CGGCGGTTGGATCGGGCGAAGGTATTCGTCGTATCCGGGAAGGGCAGGTCGATTTCGGCGCGTCGGATGTGCCGCTGTCT
CCCCAGGAAGCAAGCAAGTCCGGGTTGGTCTGCGTGCCGGCCGTCGTTACTGCTGCTGTTCCGGTGATCAACCTTCCTTC
CGTCCCGAAGGGCCAGTTGAAGCTGACCGGCGATTTGCTGGCGAAGATTTTCCTGGGCAAGGTCGATAGCTGGGATGCGC
CGGAGATCCGTGCCGTGAATCCGGGTCTCGCCCTGCCGAACCTGAAGATCCACCCCGTGGTGCGTGCCGACGGTTCGGGC
ACCACCTATCACTTCAGCGCCTATCTGAGCGAGGTGAGTGCCGAGTGGAAGTCGACATATGGCACCAAGTCCGCGTTCTC
ATGGCCGGCCGGCTACACCGGTGCCAAAGGCAGCGGCGAGGTCGTGAAGGCGGTCCAGAGCAACCGTGGCGCCATCGGCT
ATGTCGACTACAACTATGTGCTGGATGAAGGTCTGAATCCGGTGCAACTGCGCAACGCAGACGGCAACTTCGTCAGCGCC
ACGGTGGGCGGATTCCGCGATGCGGTGGTGCACAGCAACTGGAACCGGAAGGGCGACTTCACGGCCAGCCTGGTCAATCA
GGCCGGTGCCGACGCATGGCCGATCACCATGGGGACGTTCGTCGTGGTGCCGGCCGTCAGCCGCGCCGGTGAGCGCACGC
TCGAGGCCATGAAGTTCGTCACCTGGGGCTATCTCCACGGCGACCAGCTCGCCCGCGAAGCGAAGTTCGTGCCGCTGCCC
GGACAGGTGCAGGCGAACGCGTATCGGGAACTGGCGCGCGTGACGGATGCCAGCGGCAAGGCCATCGGCCTGCAAAGCAT
GGCATCGCCCGCAGGCAAGGCTCAGTAA

Upstream 100 bases:

>100_bases
TCATGCCTCGATTATTTGGTCGATCCAGACAGCAAGCCGATCCGAAAGATCTTGCTCTTGCGCCGCTACAATGCCGGCTG
CCCACTCTCCATGAACGATC

Downstream 100 bases:

>100_bases
TGTCTCGCAGTGGGATGCCCGGGCCGCATGGCCGGGCGTCCCGGCTGCTTCAATCCACGATGAACAACGTGGCACCCGTC
GAGGTACGGGACTGATGGGC

Product: Periplasmic phosphate binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MLKRTALAILGSALIMSTAVAADIQGAGSTAAAPVYRVWSTGYAAATGTRLNYAAVGSGEGIRRIREGQVDFGASDVPLS
PQEASKSGLVCVPAVVTAAVPVINLPSVPKGQLKLTGDLLAKIFLGKVDSWDAPEIRAVNPGLALPNLKIHPVVRADGSG
TTYHFSAYLSEVSAEWKSTYGTKSAFSWPAGYTGAKGSGEVVKAVQSNRGAIGYVDYNYVLDEGLNPVQLRNADGNFVSA
TVGGFRDAVVHSNWNRKGDFTASLVNQAGADAWPITMGTFVVVPAVSRAGERTLEAMKFVTWGYLHGDQLAREAKFVPLP
GQVQANAYRELARVTDASGKAIGLQSMASPAGKAQ

Sequences:

>Translated_355_residues
MLKRTALAILGSALIMSTAVAADIQGAGSTAAAPVYRVWSTGYAAATGTRLNYAAVGSGEGIRRIREGQVDFGASDVPLS
PQEASKSGLVCVPAVVTAAVPVINLPSVPKGQLKLTGDLLAKIFLGKVDSWDAPEIRAVNPGLALPNLKIHPVVRADGSG
TTYHFSAYLSEVSAEWKSTYGTKSAFSWPAGYTGAKGSGEVVKAVQSNRGAIGYVDYNYVLDEGLNPVQLRNADGNFVSA
TVGGFRDAVVHSNWNRKGDFTASLVNQAGADAWPITMGTFVVVPAVSRAGERTLEAMKFVTWGYLHGDQLAREAKFVPLP
GQVQANAYRELARVTDASGKAIGLQSMASPAGKAQ
>Mature_355_residues
MLKRTALAILGSALIMSTAVAADIQGAGSTAAAPVYRVWSTGYAAATGTRLNYAAVGSGEGIRRIREGQVDFGASDVPLS
PQEASKSGLVCVPAVVTAAVPVINLPSVPKGQLKLTGDLLAKIFLGKVDSWDAPEIRAVNPGLALPNLKIHPVVRADGSG
TTYHFSAYLSEVSAEWKSTYGTKSAFSWPAGYTGAKGSGEVVKAVQSNRGAIGYVDYNYVLDEGLNPVQLRNADGNFVSA
TVGGFRDAVVHSNWNRKGDFTASLVNQAGADAWPITMGTFVVVPAVSRAGERTLEAMKFVTWGYLHGDQLAREAKFVPLP
GQVQANAYRELARVTDASGKAIGLQSMASPAGKAQ

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=331, Percent_Identity=39.8791540785499, Blast_Score=236, Evalue=2e-63,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37128; Mature: 37128

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRTALAILGSALIMSTAVAADIQGAGSTAAAPVYRVWSTGYAAATGTRLNYAAVGSGE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCEECCCCEEEEEECCCH
GIRRIREGQVDFGASDVPLSPQEASKSGLVCVPAVVTAAVPVINLPSVPKGQLKLTGDLL
HHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEHHHHH
AKIFLGKVDSWDAPEIRAVNPGLALPNLKIHPVVRADGSGTTYHFSAYLSEVSAEWKSTY
HHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHC
GTKSAFSWPAGYTGAKGSGEVVKAVQSNRGAIGYVDYNYVLDEGLNPVQLRNADGNFVSA
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEHHHHHCCCCEEEEECCCCCEEEE
TVGGFRDAVVHSNWNRKGDFTASLVNQAGADAWPITMGTFVVVPAVSRAGERTLEAMKFV
ECCCHHHHHEECCCCCCCCHHHHHHHHCCCCCCEEECCCEEEEECHHHHHHHHHHHHHHH
TWGYLHGDQLAREAKFVPLPGQVQANAYRELARVTDASGKAIGLQSMASPAGKAQ
HHEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHCCCCCCCC
>Mature Secondary Structure
MLKRTALAILGSALIMSTAVAADIQGAGSTAAAPVYRVWSTGYAAATGTRLNYAAVGSGE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCEECCCCEEEEEECCCH
GIRRIREGQVDFGASDVPLSPQEASKSGLVCVPAVVTAAVPVINLPSVPKGQLKLTGDLL
HHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEHHHHH
AKIFLGKVDSWDAPEIRAVNPGLALPNLKIHPVVRADGSGTTYHFSAYLSEVSAEWKSTY
HHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHC
GTKSAFSWPAGYTGAKGSGEVVKAVQSNRGAIGYVDYNYVLDEGLNPVQLRNADGNFVSA
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEHHHHHCCCCEEEEECCCCCEEEE
TVGGFRDAVVHSNWNRKGDFTASLVNQAGADAWPITMGTFVVVPAVSRAGERTLEAMKFV
ECCCHHHHHEECCCCCCCCHHHHHHHHCCCCCCEEECCCEEEEECHHHHHHHHHHHHHHH
TWGYLHGDQLAREAKFVPLPGQVQANAYRELARVTDASGKAIGLQSMASPAGKAQ
HHEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6321434; 6365894; 7686882; 9278503; 9298646; 2215649; 9228942; 9671506 [H]