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The map label for this gene is gcvA [H]

Identifier: 94314505

GI number: 94314505

Start: 2339211

End: 2340212

Strand: Direct

Name: gcvA [H]

Synonym: Rmet_5586

Alternate gene names: 94314505

Gene position: 2339211-2340212 (Clockwise)

Preceding gene: 94314502

Following gene: 94314506

Centisome position: 90.66

GC content: 68.06

Gene sequence:

>1002_bases
ATGTCAGAACCGCTCGCCCGGTTGTTCTCCCTCGACCTGCTCAAGGGCTTCGTCGCCGTGGGCCGGCGCATGAGCATCAC
GCAAGCCGCCGAGGACCTCTGCCTGACCCAGTCAGCCGTCAGCCGCCAGGTCCGGGCGCTCGAAGAGCAGCTTCAAGTCC
GGCTGTTTATCCGGCGGCACCGGGGAGTCGCGTTCACGCCAGAGGGCGAGCGGCTGTTCCGCAGCGCCGACGGCGCCTTG
CTGCAACTGCAAAACGTGGCGGCGGAGCTTCGCAAGCGCGATGGCCGACAACCCGTGACGGTAACGGCCAGCATTGGCGT
GGCGGGCCTCTGGCTGTTGCCGCGACTGGGCTCGCTGCAGCGCCAGTACCCGGGCCTGGACGTGCGCCTGTCCGCCAACA
ACCAGATCAGCGATCTGCGTGCCGATGGCATCGATCTGGCGATCCGCTATTGCCCGGCATCGGCGGCGCCGCCCCTTGCC
GTCCATCTGTTCGACGAGACCATCGCCCCGGTGGCGAACCCCTCGGTGGGCGCACACCGCGTAGCGACGCCCGAGGCGCT
GGCGGAACTGCCGTTGATCGAATTCGACGATCCACGCCCATGGCTGCACTGGCGTAACTGGCTCGGCGCGCAAGGCTACC
GCCAGGCCAGCCGCCGCGGCATCCTGCGCTTCAACCAGTACGACCAGGTGATCCATGCAGCGCTGGCCGGGCAAGGCGTG
GCGCTTGGCCGCCTCGAACTGATCCAGTCGTGGATCGACGGCGGCCAGCTTGTGGTTGCCGCGACTGGTGGTGCGGCCAT
GCCCAGCCCCAACGCCTTCTGGCTGATTCACGCCAGGGAGCAACCACGCGAGGACGTCCGGCGCGTGGCGGAATGGATCC
GGAGTGAAGCCGCCGGCATAGCCGCAACCGGTGGCGCCAGCCACTCACCCCCTGCGCGCCCGACCCCTACTTTGGTGGGG
CAAGCAAACCCGCCATCAAGAAACGGGCGTCTGATCCGATAA

Upstream 100 bases:

>100_bases
ACATTTCGGGCGTTTTGTCATGCGCTGAACGCATGGCAAGAGACGGCGAGCCATGCTGTAATGGTGCCATTCGCCCCTGC
CACAGCCATACGCCCCCGTC

Downstream 100 bases:

>100_bases
GATGGTCGTTGGTCGCCGGATGGCGTCCAGCGTGTAGATGTGGAGCCACCATGGACCCCCTTTCGTTCGAGTTCGTGACT
GTCGAGGAAGCCAAGAAGGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSEPLARLFSLDLLKGFVAVGRRMSITQAAEDLCLTQSAVSRQVRALEEQLQVRLFIRRHRGVAFTPEGERLFRSADGAL
LQLQNVAAELRKRDGRQPVTVTASIGVAGLWLLPRLGSLQRQYPGLDVRLSANNQISDLRADGIDLAIRYCPASAAPPLA
VHLFDETIAPVANPSVGAHRVATPEALAELPLIEFDDPRPWLHWRNWLGAQGYRQASRRGILRFNQYDQVIHAALAGQGV
ALGRLELIQSWIDGGQLVVAATGGAAMPSPNAFWLIHAREQPREDVRRVAEWIRSEAAGIAATGGASHSPPARPTPTLVG
QANPPSRNGRLIR

Sequences:

>Translated_333_residues
MSEPLARLFSLDLLKGFVAVGRRMSITQAAEDLCLTQSAVSRQVRALEEQLQVRLFIRRHRGVAFTPEGERLFRSADGAL
LQLQNVAAELRKRDGRQPVTVTASIGVAGLWLLPRLGSLQRQYPGLDVRLSANNQISDLRADGIDLAIRYCPASAAPPLA
VHLFDETIAPVANPSVGAHRVATPEALAELPLIEFDDPRPWLHWRNWLGAQGYRQASRRGILRFNQYDQVIHAALAGQGV
ALGRLELIQSWIDGGQLVVAATGGAAMPSPNAFWLIHAREQPREDVRRVAEWIRSEAAGIAATGGASHSPPARPTPTLVG
QANPPSRNGRLIR
>Mature_332_residues
SEPLARLFSLDLLKGFVAVGRRMSITQAAEDLCLTQSAVSRQVRALEEQLQVRLFIRRHRGVAFTPEGERLFRSADGALL
QLQNVAAELRKRDGRQPVTVTASIGVAGLWLLPRLGSLQRQYPGLDVRLSANNQISDLRADGIDLAIRYCPASAAPPLAV
HLFDETIAPVANPSVGAHRVATPEALAELPLIEFDDPRPWLHWRNWLGAQGYRQASRRGILRFNQYDQVIHAALAGQGVA
LGRLELIQSWIDGGQLVVAATGGAAMPSPNAFWLIHAREQPREDVRRVAEWIRSEAAGIAATGGASHSPPARPTPTLVGQ
ANPPSRNGRLIR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=35.016835016835, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=32.404181184669, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788706, Length=256, Percent_Identity=30.078125, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787128, Length=259, Percent_Identity=25.8687258687259, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786401, Length=247, Percent_Identity=27.9352226720648, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI145693193, Length=289, Percent_Identity=26.2975778546713, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787589, Length=255, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081978, Length=302, Percent_Identity=28.1456953642384, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786713, Length=324, Percent_Identity=26.8518518518519, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787601, Length=140, Percent_Identity=30.7142857142857, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789440, Length=251, Percent_Identity=26.2948207171315, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36333; Mature: 36202

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPLARLFSLDLLKGFVAVGRRMSITQAAEDLCLTQSAVSRQVRALEEQLQVRLFIRRH
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGVAFTPEGERLFRSADGALLQLQNVAAELRKRDGRQPVTVTASIGVAGLWLLPRLGSLQ
CCCEECCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHH
RQYPGLDVRLSANNQISDLRADGIDLAIRYCPASAAPPLAVHLFDETIAPVANPSVGAHR
HHCCCCEEEECCCCCHHHHHCCCEEEEEEECCCCCCCCEEEEEHHHHHCCCCCCCCCCCC
VATPEALAELPLIEFDDPRPWLHWRNWLGAQGYRQASRRGILRFNQYDQVIHAALAGQGV
CCCHHHHHHCCCEECCCCCCHHHHHHHCCCCHHHHHHHCCCEEECHHHHHHHHHHHCCCC
ALGRLELIQSWIDGGQLVVAATGGAAMPSPNAFWLIHAREQPREDVRRVAEWIRSEAAGI
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCE
AATGGASHSPPARPTPTLVGQANPPSRNGRLIR
EECCCCCCCCCCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SEPLARLFSLDLLKGFVAVGRRMSITQAAEDLCLTQSAVSRQVRALEEQLQVRLFIRRH
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGVAFTPEGERLFRSADGALLQLQNVAAELRKRDGRQPVTVTASIGVAGLWLLPRLGSLQ
CCCEECCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHH
RQYPGLDVRLSANNQISDLRADGIDLAIRYCPASAAPPLAVHLFDETIAPVANPSVGAHR
HHCCCCEEEECCCCCHHHHHCCCEEEEEEECCCCCCCCEEEEEHHHHHCCCCCCCCCCCC
VATPEALAELPLIEFDDPRPWLHWRNWLGAQGYRQASRRGILRFNQYDQVIHAALAGQGV
CCCHHHHHHCCCEECCCCCCHHHHHHHCCCCHHHHHHHCCCEEECHHHHHHHHHHHCCCC
ALGRLELIQSWIDGGQLVVAATGGAAMPSPNAFWLIHAREQPREDVRRVAEWIRSEAAGI
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCE
AATGGASHSPPARPTPTLVGQANPPSRNGRLIR
EECCCCCCCCCCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]