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The map label for this gene is ycdT [H]

Identifier: 94314500

GI number: 94314500

Start: 2334495

End: 2336141

Strand: Reverse

Name: ycdT [H]

Synonym: Rmet_5581

Alternate gene names: 94314500

Gene position: 2336141-2334495 (Counterclockwise)

Preceding gene: 94314503

Following gene: 94314496

Centisome position: 90.55

GC content: 66.0

Gene sequence:

>1647_bases
ATGGTTCGCCCCGCACAGCCAGTCGGGGCCCCGATCTGGTTCGGCCGACCTTCCATGCCATCGTATCCTCTCGTCCTTTG
TCTGACCATCGCCGTGGTGTGCAGCGTGGGCATGGCCATTGCCGCCTGGCCGCGCCGTGACGACCCTACGGTCAGGGCGT
TCCTGCTGCTGGCCGGGGGCGTGGTGGTCTGGAGCGGCGGACGGTTGCTCGAGATCAGCGAATCGGACATGGCCGGGCGC
ATCTTCTGGGCGAAGCTCCAGTACCTCGGCATCGTGGCCGTGCCAATTGGCTGGCTCGTGGCGATGATCCATGTCAGCCG
GCCGCATTTCGTGGTGCCCTGGTCGAAGCTATGGGTGCCCGTGATGGCGGCGGTCCTGTCGGTCGTGCTCGTGTTCACCA
ATGAGCGTCACCATCTGGTCTGGACCTCGATCGAACTGATGCCCCCCGGTGTATCACCCGGTGCCGTATTCCACCACGGT
ATCGGCTACACGATCATGGCGGCGTGGACCTATGCGCTGCTCGGACTGAGCCTGTACTTCCTCATCACGGCCAAGGTGCC
GAACGCGGCGTTGTCGGTGCGCGGGCGGAATATCCTGGCGTGCGGCCTGGCGCTGCCGCTTGTTTTTCATCTCGCCTATC
TGGAGCGCCTGACTGGCCCGCTCGGCGGTGATCTTACACCGGCCACGTTCTCGGTCATGACGGTGCTGGTCTGGTTCTGT
GCGCTGCGATCGCATCTGGACGATGTCGGGCACTACGCGCGGCTGCGTGTATTCGACACCATCCGCGAAGGCTGCGTGAT
CCTCGGCCTGGACGGCACCATCGTCGAGTTCAACCCCGCAGCGGCGCGCATGTTGCCGGAACTGGCGCAAGGCGAGGTGG
CGCCTGCCACGTGGCAGGACTTGCTGTCTTCGCCGGCCACTGCCGAAGACGTCGGCACGAATCCCGGGATTCCCCAGGGC
GCGCAGTACGAACTGATGGTGGAGCCCGTCTGCCGGATGGATGGCAAGCCAATCGGCAAGCTCGTGTTCATGCGCGATAT
CAGCCGCTATCAGTCGCGCGAGCAGGCGCTGGCCATGCAGCTTGGGCAGACCGCGCAGGAGCTGTCGCGCGCCCAGGCGG
ACCTCGACGTCGATGCCCTGACCGGCATTCCGAATCGTCGCTACTTCCAGCGCGAGTCGCATGCCGCGGTGACACGCGCC
TACGAACGCGGCACGGAAATCGGCCTGCTGATTCTGGATGTCGACCTGTTCAAGCAGTTCAACGATCTCTATGGCCATCC
GCGCGGCGACGAGTGCCTGCGGCAGGTCGCGACGGCCTTGACCTGCGTGCTCGATGGCACGCAGTTTTGCGCCCGCCTGG
GTGGCGAGGAGTTTGCCGTGGTGCTGCCGGATTCCACGTCCGAGGAGACGCGAACCATCGCACGCCGGATGGTGGCAGCG
GTACGCGAACTGGGCATTCCCCATCATGGCACGCCGGTGCAGCCGTTCGTGACGATCAGCGTCGGCGCGGTCTGCGCCGT
GCCCGACGCGCCGCGCCTCGAGCCACTGCTGCATCGCGCCGACAGCGCCATGTACCGCGCCAAGCGGGCGGGCCGGAACC
GCTACGTGGTCGATGGCGGGCTCTATCTGGAGCTGCGCGGCAACTGA

Upstream 100 bases:

>100_bases
CAGCCCCAGGAGAATGATTGGCACGTTCGGTTTTCCGGATGTGTTGTGCATTGGCGGCGCCACCGGGCGCCGCGCCAGTG
TCGGACTGCGCAAGCCTATA

Downstream 100 bases:

>100_bases
CCTGGGAGGAATGCGAGAGAATTCACACGGGGGCTTGCAAATTCCTCAGGCTTCTGTAGAATTCGCGGCTCGCATCGCCC
AGATGGCGGAATTGGTAGAC

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MVRPAQPVGAPIWFGRPSMPSYPLVLCLTIAVVCSVGMAIAAWPRRDDPTVRAFLLLAGGVVVWSGGRLLEISESDMAGR
IFWAKLQYLGIVAVPIGWLVAMIHVSRPHFVVPWSKLWVPVMAAVLSVVLVFTNERHHLVWTSIELMPPGVSPGAVFHHG
IGYTIMAAWTYALLGLSLYFLITAKVPNAALSVRGRNILACGLALPLVFHLAYLERLTGPLGGDLTPATFSVMTVLVWFC
ALRSHLDDVGHYARLRVFDTIREGCVILGLDGTIVEFNPAAARMLPELAQGEVAPATWQDLLSSPATAEDVGTNPGIPQG
AQYELMVEPVCRMDGKPIGKLVFMRDISRYQSREQALAMQLGQTAQELSRAQADLDVDALTGIPNRRYFQRESHAAVTRA
YERGTEIGLLILDVDLFKQFNDLYGHPRGDECLRQVATALTCVLDGTQFCARLGGEEFAVVLPDSTSEETRTIARRMVAA
VRELGIPHHGTPVQPFVTISVGAVCAVPDAPRLEPLLHRADSAMYRAKRAGRNRYVVDGGLYLELRGN

Sequences:

>Translated_548_residues
MVRPAQPVGAPIWFGRPSMPSYPLVLCLTIAVVCSVGMAIAAWPRRDDPTVRAFLLLAGGVVVWSGGRLLEISESDMAGR
IFWAKLQYLGIVAVPIGWLVAMIHVSRPHFVVPWSKLWVPVMAAVLSVVLVFTNERHHLVWTSIELMPPGVSPGAVFHHG
IGYTIMAAWTYALLGLSLYFLITAKVPNAALSVRGRNILACGLALPLVFHLAYLERLTGPLGGDLTPATFSVMTVLVWFC
ALRSHLDDVGHYARLRVFDTIREGCVILGLDGTIVEFNPAAARMLPELAQGEVAPATWQDLLSSPATAEDVGTNPGIPQG
AQYELMVEPVCRMDGKPIGKLVFMRDISRYQSREQALAMQLGQTAQELSRAQADLDVDALTGIPNRRYFQRESHAAVTRA
YERGTEIGLLILDVDLFKQFNDLYGHPRGDECLRQVATALTCVLDGTQFCARLGGEEFAVVLPDSTSEETRTIARRMVAA
VRELGIPHHGTPVQPFVTISVGAVCAVPDAPRLEPLLHRADSAMYRAKRAGRNRYVVDGGLYLELRGN
>Mature_548_residues
MVRPAQPVGAPIWFGRPSMPSYPLVLCLTIAVVCSVGMAIAAWPRRDDPTVRAFLLLAGGVVVWSGGRLLEISESDMAGR
IFWAKLQYLGIVAVPIGWLVAMIHVSRPHFVVPWSKLWVPVMAAVLSVVLVFTNERHHLVWTSIELMPPGVSPGAVFHHG
IGYTIMAAWTYALLGLSLYFLITAKVPNAALSVRGRNILACGLALPLVFHLAYLERLTGPLGGDLTPATFSVMTVLVWFC
ALRSHLDDVGHYARLRVFDTIREGCVILGLDGTIVEFNPAAARMLPELAQGEVAPATWQDLLSSPATAEDVGTNPGIPQG
AQYELMVEPVCRMDGKPIGKLVFMRDISRYQSREQALAMQLGQTAQELSRAQADLDVDALTGIPNRRYFQRESHAAVTRA
YERGTEIGLLILDVDLFKQFNDLYGHPRGDECLRQVATALTCVLDGTQFCARLGGEEFAVVLPDSTSEETRTIARRMVAA
VRELGIPHHGTPVQPFVTISVGAVCAVPDAPRLEPLLHRADSAMYRAKRAGRNRYVVDGGLYLELRGN

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=37.6543209876543, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI145693134, Length=180, Percent_Identity=37.2222222222222, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=34.6368715083799, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1788381, Length=305, Percent_Identity=27.2131147540984, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787541, Length=180, Percent_Identity=32.2222222222222, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87081881, Length=157, Percent_Identity=35.031847133758, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=36.4779874213836, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=28.4883720930233, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081977, Length=153, Percent_Identity=32.0261437908497, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=28.0701754385965, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788956, Length=190, Percent_Identity=32.6315789473684, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=29.4478527607362, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081974, Length=157, Percent_Identity=26.7515923566879, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59916; Mature: 59916

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRPAQPVGAPIWFGRPSMPSYPLVLCLTIAVVCSVGMAIAAWPRRDDPTVRAFLLLAGG
CCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHHCC
VVVWSGGRLLEISESDMAGRIFWAKLQYLGIVAVPIGWLVAMIHVSRPHFVVPWSKLWVP
EEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHH
VMAAVLSVVLVFTNERHHLVWTSIELMPPGVSPGAVFHHGIGYTIMAAWTYALLGLSLYF
HHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
LITAKVPNAALSVRGRNILACGLALPLVFHLAYLERLTGPLGGDLTPATFSVMTVLVWFC
HEEECCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
ALRSHLDDVGHYARLRVFDTIREGCVILGLDGTIVEFNPAAARMLPELAQGEVAPATWQD
HHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECHHHHHHHHHHHCCCCCCHHHHH
LLSSPATAEDVGTNPGIPQGAQYELMVEPVCRMDGKPIGKLVFMRDISRYQSREQALAMQ
HHCCCCCHHHCCCCCCCCCCCCEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGQTAQELSRAQADLDVDALTGIPNRRYFQRESHAAVTRAYERGTEIGLLILDVDLFKQF
HHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHH
NDLYGHPRGDECLRQVATALTCVLDGTQFCARLGGEEFAVVLPDSTSEETRTIARRMVAA
HHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHH
VRELGIPHHGTPVQPFVTISVGAVCAVPDAPRLEPLLHRADSAMYRAKRAGRNRYVVDGG
HHHCCCCCCCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECE
LYLELRGN
EEEEECCC
>Mature Secondary Structure
MVRPAQPVGAPIWFGRPSMPSYPLVLCLTIAVVCSVGMAIAAWPRRDDPTVRAFLLLAGG
CCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHHCC
VVVWSGGRLLEISESDMAGRIFWAKLQYLGIVAVPIGWLVAMIHVSRPHFVVPWSKLWVP
EEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHH
VMAAVLSVVLVFTNERHHLVWTSIELMPPGVSPGAVFHHGIGYTIMAAWTYALLGLSLYF
HHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
LITAKVPNAALSVRGRNILACGLALPLVFHLAYLERLTGPLGGDLTPATFSVMTVLVWFC
HEEECCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
ALRSHLDDVGHYARLRVFDTIREGCVILGLDGTIVEFNPAAARMLPELAQGEVAPATWQD
HHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECHHHHHHHHHHHCCCCCCHHHHH
LLSSPATAEDVGTNPGIPQGAQYELMVEPVCRMDGKPIGKLVFMRDISRYQSREQALAMQ
HHCCCCCHHHCCCCCCCCCCCCEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGQTAQELSRAQADLDVDALTGIPNRRYFQRESHAAVTRAYERGTEIGLLILDVDLFKQF
HHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHH
NDLYGHPRGDECLRQVATALTCVLDGTQFCARLGGEEFAVVLPDSTSEETRTIARRMVAA
HHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHH
VRELGIPHHGTPVQPFVTISVGAVCAVPDAPRLEPLLHRADSAMYRAKRAGRNRYVVDGG
HHHCCCCCCCCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECE
LYLELRGN
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]