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The map label for this gene is gcvA [H]

Identifier: 94314492

GI number: 94314492

Start: 2326939

End: 2327844

Strand: Reverse

Name: gcvA [H]

Synonym: Rmet_5573

Alternate gene names: 94314492

Gene position: 2327844-2326939 (Counterclockwise)

Preceding gene: 94314496

Following gene: 94314491

Centisome position: 90.22

GC content: 66.67

Gene sequence:

>906_bases
ATGCGCCAACGTCTTCCGAGCCTGTCCAACCTGCGTGCCTTCGACGCGGCGGCGCTGCATCTGAATCTGCGGCGCGCGGC
GGCGGATCTGTACATCACGGAGAGCGCGGTATCGCGCCAGATCGCCTCGCTGGAAAGCCTGCTTGGCGTGCAGCTTTTTC
ACCGGGCCAACCAGCGCATCACGCTGACGGCGGCGGGGGTGCTCTACAGCCGCCAGGTGCGCGAGATGCTGCGCAAGCTG
CAGCGCGACACGCTGGACATCATGGCGCACGAAGGTGTGGGTGGCACGGTGGAACTGGCGTGCGTGCCAACACTGGCCGT
CGAGTGGCTGATTCCACGGCTGCCGGGTTTCTATGCCCGCCACCCGCAGGTCATCGTCAACATCCGCGCGCAGTCGGATG
TGTTCCTGTTCGAGGGCACGCCGTACGACGCGGCGATCCACTTCGGCGAGGCGGTCTATCCGGGCGCGCGGGTGGACCGG
ATGTTCGGCGAGGAGTCGGTCGCCGTCTGCCATCCGGACTTTTTCGGCGGCAAGAAGCGGGTCAGCGTCAACGACATCGC
GCGATCGCCGCTACTGCACCTGGTCACGCGCAGCTTCGGCTGGAAGCAATGGATGGAGGCCGCCGGCATTGACGATGTCA
ATGCGCTGCGCGGCGCGCGCTATGACCACCACAGCATGGTCATCAGCGCGGCCCGCGCGGGGCTTGGCGTCGGGTTGGTG
CCGCGTTTCCTGGTGGATGAGTACCTGACGCGCGGGCTGCTGGTGACACCCGTGGCGCGATCACTGCGCGCGACGAGTGC
GTACTACCTCGTGGTGCCCGACACGCAGCCAATGAGCGATGCGATGGGGCACCTGCGCGAATGGTTGCTGGAAGCAGCGG
GCGAGTTCGTGGGCGTTGCGACATAG

Upstream 100 bases:

>100_bases
GGCGGTGCGAGTTCGAATCGTTCCTCATCAAGCTGGCGGTTTCAAGCGAAATTTCGGTATAGGATGATGACTTTTAGTCA
ACAAGTCCCTCCCCCACCCC

Downstream 100 bases:

>100_bases
AGGGAGCCTCGAGGGAACGACCTTGTGACTTTGCGTTGTGACTTCGCGTTGCGCCAAGGCTTGAGTGCCGTATACGATGG
GGTGCGGAACATGGTCGTGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRQRLPSLSNLRAFDAAALHLNLRRAAADLYITESAVSRQIASLESLLGVQLFHRANQRITLTAAGVLYSRQVREMLRKL
QRDTLDIMAHEGVGGTVELACVPTLAVEWLIPRLPGFYARHPQVIVNIRAQSDVFLFEGTPYDAAIHFGEAVYPGARVDR
MFGEESVAVCHPDFFGGKKRVSVNDIARSPLLHLVTRSFGWKQWMEAAGIDDVNALRGARYDHHSMVISAARAGLGVGLV
PRFLVDEYLTRGLLVTPVARSLRATSAYYLVVPDTQPMSDAMGHLREWLLEAAGEFVGVAT

Sequences:

>Translated_301_residues
MRQRLPSLSNLRAFDAAALHLNLRRAAADLYITESAVSRQIASLESLLGVQLFHRANQRITLTAAGVLYSRQVREMLRKL
QRDTLDIMAHEGVGGTVELACVPTLAVEWLIPRLPGFYARHPQVIVNIRAQSDVFLFEGTPYDAAIHFGEAVYPGARVDR
MFGEESVAVCHPDFFGGKKRVSVNDIARSPLLHLVTRSFGWKQWMEAAGIDDVNALRGARYDHHSMVISAARAGLGVGLV
PRFLVDEYLTRGLLVTPVARSLRATSAYYLVVPDTQPMSDAMGHLREWLLEAAGEFVGVAT
>Mature_301_residues
MRQRLPSLSNLRAFDAAALHLNLRRAAADLYITESAVSRQIASLESLLGVQLFHRANQRITLTAAGVLYSRQVREMLRKL
QRDTLDIMAHEGVGGTVELACVPTLAVEWLIPRLPGFYARHPQVIVNIRAQSDVFLFEGTPYDAAIHFGEAVYPGARVDR
MFGEESVAVCHPDFFGGKKRVSVNDIARSPLLHLVTRSFGWKQWMEAAGIDDVNALRGARYDHHSMVISAARAGLGVGLV
PRFLVDEYLTRGLLVTPVARSLRATSAYYLVVPDTQPMSDAMGHLREWLLEAAGEFVGVAT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=300, Percent_Identity=35.3333333333333, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1786448, Length=285, Percent_Identity=30.8771929824561, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788706, Length=284, Percent_Identity=29.5774647887324, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=25, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790262, Length=250, Percent_Identity=26.4, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI145693105, Length=180, Percent_Identity=30, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786401, Length=242, Percent_Identity=23.1404958677686, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=127, Percent_Identity=31.496062992126, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787530, Length=250, Percent_Identity=27.6, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33276; Mature: 33276

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQRLPSLSNLRAFDAAALHLNLRRAAADLYITESAVSRQIASLESLLGVQLFHRANQRI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCEE
TLTAAGVLYSRQVREMLRKLQRDTLDIMAHEGVGGTVELACVPTLAVEWLIPRLPGFYAR
EEEEHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEHHHHHHHHHHHCCCCCCCC
HPQVIVNIRAQSDVFLFEGTPYDAAIHFGEAVYPGARVDRMFGEESVAVCHPDFFGGKKR
CCEEEEEEECCCCEEEEECCCCHHHEECCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCE
VSVNDIARSPLLHLVTRSFGWKQWMEAAGIDDVNALRGARYDHHSMVISAARAGLGVGLV
EEHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHH
PRFLVDEYLTRGLLVTPVARSLRATSAYYLVVPDTQPMSDAMGHLREWLLEAAGEFVGVA
HHHHHHHHHHCCEEHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEECC
T
C
>Mature Secondary Structure
MRQRLPSLSNLRAFDAAALHLNLRRAAADLYITESAVSRQIASLESLLGVQLFHRANQRI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCEE
TLTAAGVLYSRQVREMLRKLQRDTLDIMAHEGVGGTVELACVPTLAVEWLIPRLPGFYAR
EEEEHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEHHHHHHHHHHHCCCCCCCC
HPQVIVNIRAQSDVFLFEGTPYDAAIHFGEAVYPGARVDRMFGEESVAVCHPDFFGGKKR
CCEEEEEEECCCCEEEEECCCCHHHEECCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCE
VSVNDIARSPLLHLVTRSFGWKQWMEAAGIDDVNALRGARYDHHSMVISAARAGLGVGLV
EEHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHH
PRFLVDEYLTRGLLVTPVARSLRATSAYYLVVPDTQPMSDAMGHLREWLLEAAGEFVGVA
HHHHHHHHHHCCEEHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEECC
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]