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The map label for this gene is baiB [H]

Identifier: 94314447

GI number: 94314447

Start: 2274161

End: 2275690

Strand: Reverse

Name: baiB [H]

Synonym: Rmet_5528

Alternate gene names: 94314447

Gene position: 2275690-2274161 (Counterclockwise)

Preceding gene: 94314448

Following gene: 94314446

Centisome position: 88.2

GC content: 57.12

Gene sequence:

>1530_bases
ATGAGCGAAGCCACAATCTATCTGGATGGCCGCGCGAAAGCGCGAGATGAGGTCCTGCAGCAAGCAATGCGCGCTGCCAC
GGGATTTGACAAGATGGGAATCCGCGAGGGGGACACCGTCGCCTCGCTCTTGCGCAATGATTTCTCGTTCTTTGAGGTCC
AACAGGCTGCGGCAGCCGTCGGGGCGTATTCAGTTCCATTGAACTGGCACGGCAAGACTGAGGAGCTCACCTATATCTTG
AATGATGCAAAGCCCAAGGTGCTGGTAGCTCACGCAGACTTGCTAGAGCCCTTGCGGCCGCACATTCCACAAGGCTTGCA
GGTGCTGGTAGTGCCGACGCCTCCGGAGGTTCAGTCTCGTTACGGGATCTCGGATGCTCTGGCAACGCCAAAGGCGGGGG
ACATTGGTTGGCCAGAGTGGTGCCAGACGTTTGAACTCTGGACTCAGCCTCCAAAGCGCGGGCGGGCCACCATGATCTAC
ACGTCCGGCACCACGGGTCATCCCAAAGGTGTGAAACGCGATCCGGCAACGCCGGAGCAGGCCAAGGCTTATGTGGACAT
CATCGAACGCGTTTACGGCCTGACGCCGGGCGTCAGGGCACTCATTACCGGACCTCTTTATCACGCATCGCCGAATGCTT
ATGGCCGCCAAGCGATCACGGCTGCAGATGTCCTGGTGCTGCAATCGAAGTTCGACCCCGAGGAGACCCTGGCAGCCATT
GAAAAGTATCGCATTACCAATGCGGTGATGGTGCCGACTATGTTCATCCGCATTCTTAAGCTGCCCAAAGAGGTGCGCGA
GCGATACGATGTCAGCTCGTTGAAGTGGGTAACACATACCGGCGCTCCGTGTCCTCGTGAGGTGAAGCAGGAGCTCATGG
AGTGGTGGGGACCGGTCGTCTACGAGACCTACGGTGGCACCGAGGTTGGTACCGCAACGCTTGCCACCCCGGACGATTGG
CTCAATCACCCTGGGTCAGTTGGTGTGCCTACGCCGGGTACTCAAATCGCCTTCTTTGGTGAGGATGGGAAGCCAGTAGA
GGATGGAACTCCTGGTGAGATTTATATGCGCGTGCCTGCGTACGCGGACTTCACGTATCTCAATCACGAAGAGAAGCGAA
AGAGCGTAGAGCGAGACGGCTTGATCAGCGTTGGCGATGTTGGCTACCTGAAGGAAGGCCGTCTATATCTGTGCGATAGG
CGCTCGGACATGGTTATTTCTGGGGGTACCAATATCTACCCTGCCGAAATTGAGATGGTGCTCACGCAGTGTCCGGGGGT
CCATGATTGCGCTGTGTTTGGGATACCAGATGAGGATTTCGGGGAATCGTTGGCCGCTGCTGTGGAACTTATGCCAGGTG
CCGAGCTAAGCGCCAGCGACATTCAGAAGTATCTGGAATCGCATCTCGCGAAGTACAAGGTGCCAAGGCGCATCGACTTC
CATGCATCGTTGCCGCGAGAGGACAGTGGCAAGATATTCAAACGTCGACTCCGAGATCCGTTCTGGCAGGCTGTTGGCCG
GAAAATCTGA

Upstream 100 bases:

>100_bases
TTGACCAACTTTTCGGCGATGGAGAGGCGCATACGGCAAGACTGGCCTCGCTGCTTCACGCATAGCAGCCCAACAAATAC
TACATGGAGGGAGACAGATT

Downstream 100 bases:

>100_bases
TGTCGGAGCAGACGACAGACCAAAAGCTTCTGGCGTCGCAACTCGAGCGGGCACTCCTTCGGGAAGAAGGGGTGCCTGGC
GAGATCGCTAACCTTCGGCG

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSEATIYLDGRAKARDEVLQQAMRAATGFDKMGIREGDTVASLLRNDFSFFEVQQAAAAVGAYSVPLNWHGKTEELTYIL
NDAKPKVLVAHADLLEPLRPHIPQGLQVLVVPTPPEVQSRYGISDALATPKAGDIGWPEWCQTFELWTQPPKRGRATMIY
TSGTTGHPKGVKRDPATPEQAKAYVDIIERVYGLTPGVRALITGPLYHASPNAYGRQAITAADVLVLQSKFDPEETLAAI
EKYRITNAVMVPTMFIRILKLPKEVRERYDVSSLKWVTHTGAPCPREVKQELMEWWGPVVYETYGGTEVGTATLATPDDW
LNHPGSVGVPTPGTQIAFFGEDGKPVEDGTPGEIYMRVPAYADFTYLNHEEKRKSVERDGLISVGDVGYLKEGRLYLCDR
RSDMVISGGTNIYPAEIEMVLTQCPGVHDCAVFGIPDEDFGESLAAAVELMPGAELSASDIQKYLESHLAKYKVPRRIDF
HASLPREDSGKIFKRRLRDPFWQAVGRKI

Sequences:

>Translated_509_residues
MSEATIYLDGRAKARDEVLQQAMRAATGFDKMGIREGDTVASLLRNDFSFFEVQQAAAAVGAYSVPLNWHGKTEELTYIL
NDAKPKVLVAHADLLEPLRPHIPQGLQVLVVPTPPEVQSRYGISDALATPKAGDIGWPEWCQTFELWTQPPKRGRATMIY
TSGTTGHPKGVKRDPATPEQAKAYVDIIERVYGLTPGVRALITGPLYHASPNAYGRQAITAADVLVLQSKFDPEETLAAI
EKYRITNAVMVPTMFIRILKLPKEVRERYDVSSLKWVTHTGAPCPREVKQELMEWWGPVVYETYGGTEVGTATLATPDDW
LNHPGSVGVPTPGTQIAFFGEDGKPVEDGTPGEIYMRVPAYADFTYLNHEEKRKSVERDGLISVGDVGYLKEGRLYLCDR
RSDMVISGGTNIYPAEIEMVLTQCPGVHDCAVFGIPDEDFGESLAAAVELMPGAELSASDIQKYLESHLAKYKVPRRIDF
HASLPREDSGKIFKRRLRDPFWQAVGRKI
>Mature_508_residues
SEATIYLDGRAKARDEVLQQAMRAATGFDKMGIREGDTVASLLRNDFSFFEVQQAAAAVGAYSVPLNWHGKTEELTYILN
DAKPKVLVAHADLLEPLRPHIPQGLQVLVVPTPPEVQSRYGISDALATPKAGDIGWPEWCQTFELWTQPPKRGRATMIYT
SGTTGHPKGVKRDPATPEQAKAYVDIIERVYGLTPGVRALITGPLYHASPNAYGRQAITAADVLVLQSKFDPEETLAAIE
KYRITNAVMVPTMFIRILKLPKEVRERYDVSSLKWVTHTGAPCPREVKQELMEWWGPVVYETYGGTEVGTATLATPDDWL
NHPGSVGVPTPGTQIAFFGEDGKPVEDGTPGEIYMRVPAYADFTYLNHEEKRKSVERDGLISVGDVGYLKEGRLYLCDRR
SDMVISGGTNIYPAEIEMVLTQCPGVHDCAVFGIPDEDFGESLAAAVELMPGAELSASDIQKYLESHLAKYKVPRRIDFH
ASLPREDSGKIFKRRLRDPFWQAVGRKI

Specific function: Active with a large number of C-24 bile acids possessing unhindered C-24 carboxyl groups. Requires ATP and produces AMP and pyrophosphate in addition to the bile-acid CoA thioester [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=522, Percent_Identity=26.0536398467433, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI187761343, Length=522, Percent_Identity=26.0536398467433, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI156151445, Length=504, Percent_Identity=26.7857142857143, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI42544132, Length=368, Percent_Identity=27.445652173913, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI38505220, Length=518, Percent_Identity=25.0965250965251, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI122937307, Length=495, Percent_Identity=25.0505050505051, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI58082049, Length=541, Percent_Identity=24.0295748613678, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI157311624, Length=516, Percent_Identity=24.2248062015504, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI157311622, Length=516, Percent_Identity=24.2248062015504, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI115511026, Length=512, Percent_Identity=25.5859375, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI28416953, Length=510, Percent_Identity=25.6862745098039, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI8923896, Length=396, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI253970489, Length=396, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1788107, Length=519, Percent_Identity=26.9749518304432, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI221142682, Length=497, Percent_Identity=27.9678068410463, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI145693145, Length=489, Percent_Identity=28.2208588957055, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1788595, Length=333, Percent_Identity=26.1261261261261, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786810, Length=517, Percent_Identity=24.1779497098646, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790505, Length=518, Percent_Identity=23.1660231660232, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=516, Percent_Identity=27.7131782945736, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17531443, Length=513, Percent_Identity=26.9005847953216, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17558820, Length=508, Percent_Identity=27.3622047244095, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=508, Percent_Identity=28.1496062992126, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17559526, Length=521, Percent_Identity=26.1036468330134, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71994703, Length=529, Percent_Identity=26.4650283553875, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71994690, Length=529, Percent_Identity=26.4650283553875, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=529, Percent_Identity=26.4650283553875, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=527, Percent_Identity=24.4781783681214, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=529, Percent_Identity=24.1965973534972, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=370, Percent_Identity=26.2162162162162, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=346, Percent_Identity=25.4335260115607, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71996755, Length=500, Percent_Identity=23.6, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25141359, Length=358, Percent_Identity=24.0223463687151, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=512, Percent_Identity=25.78125, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6319264, Length=377, Percent_Identity=26.525198938992, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323182, Length=544, Percent_Identity=23.8970588235294, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21355181, Length=501, Percent_Identity=27.3453093812375, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI281365686, Length=468, Percent_Identity=27.1367521367521, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24648253, Length=474, Percent_Identity=27.0042194092827, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24648260, Length=514, Percent_Identity=24.7081712062257, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648255, Length=474, Percent_Identity=27.0042194092827, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24581924, Length=531, Percent_Identity=25.9887005649718, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21358303, Length=471, Percent_Identity=25.6900212314225, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI21356441, Length=472, Percent_Identity=25.4237288135593, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653035, Length=515, Percent_Identity=24.2718446601942, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24656500, Length=498, Percent_Identity=25.7028112449799, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18859661, Length=359, Percent_Identity=29.5264623955432, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI161076582, Length=522, Percent_Identity=23.1800766283525, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922652, Length=505, Percent_Identity=23.7623762376238, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21356947, Length=505, Percent_Identity=23.7623762376238, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI20130357, Length=363, Percent_Identity=25.6198347107438, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI221329755, Length=521, Percent_Identity=22.4568138195777, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648257, Length=517, Percent_Identity=21.0831721470019, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24667955, Length=364, Percent_Identity=27.7472527472527, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24667959, Length=364, Percent_Identity=27.7472527472527, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI62472339, Length=364, Percent_Identity=27.7472527472527, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21355225, Length=364, Percent_Identity=26.0989010989011, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648676, Length=384, Percent_Identity=23.4375, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 56405; Mature: 56274

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEATIYLDGRAKARDEVLQQAMRAATGFDKMGIREGDTVASLLRNDFSFFEVQQAAAAV
CCCCEEEECCCCHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
GAYSVPLNWHGKTEELTYILNDAKPKVLVAHADLLEPLRPHIPQGLQVLVVPTPPEVQSR
CCEECCCCCCCCCCEEEEEECCCCCEEEEEEHHHHHHHHHCCCCCCEEEEECCCCHHHHH
YGISDALATPKAGDIGWPEWCQTFELWTQPPKRGRATMIYTSGTTGHPKGVKRDPATPEQ
CCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHH
AKAYVDIIERVYGLTPGVRALITGPLYHASPNAYGRQAITAADVLVLQSKFDPEETLAAI
HHHHHHHHHHHHCCCCCCHHHEECCCEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
EKYRITNAVMVPTMFIRILKLPKEVRERYDVSSLKWVTHTGAPCPREVKQELMEWWGPVV
HHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEE
YETYGGTEVGTATLATPDDWLNHPGSVGVPTPGTQIAFFGEDGKPVEDGTPGEIYMRVPA
EEECCCCCCCEEEECCCHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECC
YADFTYLNHEEKRKSVERDGLISVGDVGYLKEGRLYLCDRRSDMVISGGTNIYPAEIEMV
CCCEEECCCHHHHHHHHHCCCEEECCCCEECCCCEEEEECCCCEEEECCCEECHHHHHHH
LTQCPGVHDCAVFGIPDEDFGESLAAAVELMPGAELSASDIQKYLESHLAKYKVPRRIDF
HHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEC
HASLPREDSGKIFKRRLRDPFWQAVGRKI
CCCCCCCCCHHHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure 
SEATIYLDGRAKARDEVLQQAMRAATGFDKMGIREGDTVASLLRNDFSFFEVQQAAAAV
CCCEEEECCCCHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
GAYSVPLNWHGKTEELTYILNDAKPKVLVAHADLLEPLRPHIPQGLQVLVVPTPPEVQSR
CCEECCCCCCCCCCEEEEEECCCCCEEEEEEHHHHHHHHHCCCCCCEEEEECCCCHHHHH
YGISDALATPKAGDIGWPEWCQTFELWTQPPKRGRATMIYTSGTTGHPKGVKRDPATPEQ
CCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHH
AKAYVDIIERVYGLTPGVRALITGPLYHASPNAYGRQAITAADVLVLQSKFDPEETLAAI
HHHHHHHHHHHHCCCCCCHHHEECCCEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
EKYRITNAVMVPTMFIRILKLPKEVRERYDVSSLKWVTHTGAPCPREVKQELMEWWGPVV
HHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEE
YETYGGTEVGTATLATPDDWLNHPGSVGVPTPGTQIAFFGEDGKPVEDGTPGEIYMRVPA
EEECCCCCCCEEEECCCHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECC
YADFTYLNHEEKRKSVERDGLISVGDVGYLKEGRLYLCDRRSDMVISGGTNIYPAEIEMV
CCCEEECCCHHHHHHHHHCCCEEECCCCEECCCCEEEEECCCCEEEECCCEECHHHHHHH
LTQCPGVHDCAVFGIPDEDFGESLAAAVELMPGAELSASDIQKYLESHLAKYKVPRRIDF
HHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEC
HASLPREDSGKIFKRRLRDPFWQAVGRKI
CCCCCCCCCHHHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254270; 1551828 [H]