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The map label for this gene is crt [H]

Identifier: 94314440

GI number: 94314440

Start: 2267685

End: 2268506

Strand: Reverse

Name: crt [H]

Synonym: Rmet_5521

Alternate gene names: 94314440

Gene position: 2268506-2267685 (Counterclockwise)

Preceding gene: 94314441

Following gene: 94314439

Centisome position: 87.92

GC content: 58.39

Gene sequence:

>822_bases
ATGAACGATTCGGATGATGTCAAACTGACCTACGACGGAGGCATCGCCCTCGTTACGTTGAACCGTCCTCAGGCAAAGAA
CGCGCTGACGCCCGCAATGACGGTTGCGCTGACGGAGATGTTCCGCTCATTCCGCTCCGATGAGCAAGTGCGGGTCGTCG
TCTTCGCAGGAGCTGGGGCTGACTTTTGCTCGGGCGGTGATGTCAAGGCAATGGGAGGTGGAGCGCCGCGAACTACCGAG
CAACGCCGCCAAGGGATGGCGCCTTACCGAGACCTTGTCTTGGCTGTATCGGCTTTGGATAAGCCAGTGATCGCCGCAGT
TGATGGCGTTGCCTACGGCGCAGGACTAAGCCTGGCGCTTCTTGCGGACATTGTCCTTTGCTCGTATCGCGCGCGTATGG
CTGCGGTATTTCATCGAATTGGCCTGGTGCCAGATGTCGGTGCCTGGTACACCCTTCCGCGTGTGGTGGGTCTTCAACGC
GCAAAGGAATTGGTGTTCTCCGCGCGAGAGTTTGGCTCCGACGAGGCGAAACGGATGGGGCTGGCCATGGAGGTTCTGGC
ACCTGAAGCGTTGATGACGCGGGCTATCGAGATTGCACGTAGCTTCGAAAGTGCGTCCGGTACGGCGATGAGCCTCTCCA
AACAGGCGCTGCAGAAGTCGCTCCAGTGCGACCTCGAGACGATGCTGGATCTGGAGGCTACAGGTCAGGCGATTGCTGCG
GGTAGCGATTACACGCGTGAGTCTATCAGGCGCTTTGCTGCAAAAGAATCTCCTCAATTCCGTTGGGCTCCCTTGCCCGC
AACCGAGTACAAAAATGATTGA

Upstream 100 bases:

>100_bases
TGACGGAGTACACCTGCGAAGGCAGGACTGTGCTAGGCATGACTGAGTATTGCGACATCATTCAAGATGGTGTTCCACTT
TCTGTGCGGCAAGGACGAGC

Downstream 100 bases:

>100_bases
CTATCAAAAAACGAAGGCCTGGCAGTCGGGTGACGTGATCCACAACTACACGGAGAAGGACAGCATTCTCTATGCGTTGG
GTCTGGGAATTGGCTCTGAT

Product: Enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNDSDDVKLTYDGGIALVTLNRPQAKNALTPAMTVALTEMFRSFRSDEQVRVVVFAGAGADFCSGGDVKAMGGGAPRTTE
QRRQGMAPYRDLVLAVSALDKPVIAAVDGVAYGAGLSLALLADIVLCSYRARMAAVFHRIGLVPDVGAWYTLPRVVGLQR
AKELVFSAREFGSDEAKRMGLAMEVLAPEALMTRAIEIARSFESASGTAMSLSKQALQKSLQCDLETMLDLEATGQAIAA
GSDYTRESIRRFAAKESPQFRWAPLPATEYKND

Sequences:

>Translated_273_residues
MNDSDDVKLTYDGGIALVTLNRPQAKNALTPAMTVALTEMFRSFRSDEQVRVVVFAGAGADFCSGGDVKAMGGGAPRTTE
QRRQGMAPYRDLVLAVSALDKPVIAAVDGVAYGAGLSLALLADIVLCSYRARMAAVFHRIGLVPDVGAWYTLPRVVGLQR
AKELVFSAREFGSDEAKRMGLAMEVLAPEALMTRAIEIARSFESASGTAMSLSKQALQKSLQCDLETMLDLEATGQAIAA
GSDYTRESIRRFAAKESPQFRWAPLPATEYKND
>Mature_273_residues
MNDSDDVKLTYDGGIALVTLNRPQAKNALTPAMTVALTEMFRSFRSDEQVRVVVFAGAGADFCSGGDVKAMGGGAPRTTE
QRRQGMAPYRDLVLAVSALDKPVIAAVDGVAYGAGLSLALLADIVLCSYRARMAAVFHRIGLVPDVGAWYTLPRVVGLQR
AKELVFSAREFGSDEAKRMGLAMEVLAPEALMTRAIEIARSFESASGTAMSLSKQALQKSLQCDLETMLDLEATGQAIAA
GSDYTRESIRRFAAKESPQFRWAPLPATEYKND

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=27.9527559055118, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI4502327, Length=270, Percent_Identity=29.6296296296296, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI70995211, Length=184, Percent_Identity=33.1521739130435, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI45643119, Length=212, Percent_Identity=26.4150943396226, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI260275230, Length=212, Percent_Identity=26.4150943396226, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI260274832, Length=212, Percent_Identity=26.4150943396226, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI37594469, Length=160, Percent_Identity=31.25, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI37594471, Length=160, Percent_Identity=31.25, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI116235487, Length=231, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI31542718, Length=259, Percent_Identity=27.4131274131274, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI68989263, Length=210, Percent_Identity=27.6190476190476, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221136756, Length=260, Percent_Identity=23.0769230769231, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221136753, Length=260, Percent_Identity=23.0769230769231, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221307494, Length=260, Percent_Identity=23.8461538461538, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI25453481, Length=253, Percent_Identity=24.1106719367589, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI51538573, Length=253, Percent_Identity=24.1106719367589, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI4757968, Length=254, Percent_Identity=23.6220472440945, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI49355787, Length=254, Percent_Identity=23.6220472440945, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI20127408, Length=185, Percent_Identity=25.4054054054054, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4757966, Length=179, Percent_Identity=27.3743016759777, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI51538581, Length=179, Percent_Identity=27.3743016759777, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI221142681, Length=224, Percent_Identity=34.375, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=35.8267716535433, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=30.9236947791165, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=27.6679841897233, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=28.2828282828283, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=25.2631578947368, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082183, Length=252, Percent_Identity=19.8412698412698, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=32.4324324324324, Blast_Score=113, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=30.7692307692308, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=222, Percent_Identity=28.3783783783784, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=27.2727272727273, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17549921, Length=244, Percent_Identity=29.5081967213115, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=237, Percent_Identity=24.0506329113924, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=253, Percent_Identity=26.8774703557312, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=214, Percent_Identity=28.5046728971963, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25144160, Length=226, Percent_Identity=28.3185840707965, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=226, Percent_Identity=28.3185840707965, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=220, Percent_Identity=25, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=32.0158102766798, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=29.9595141700405, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=29.9595141700405, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19920382, Length=224, Percent_Identity=31.25, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357171, Length=201, Percent_Identity=29.3532338308458, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24654903, Length=179, Percent_Identity=27.3743016759777, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24650670, Length=195, Percent_Identity=29.7435897435897, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571730, Length=156, Percent_Identity=32.0512820512821, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571729, Length=156, Percent_Identity=32.0512820512821, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 29364; Mature: 29364

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSDDVKLTYDGGIALVTLNRPQAKNALTPAMTVALTEMFRSFRSDEQVRVVVFAGAGA
CCCCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
DFCSGGDVKAMGGGAPRTTEQRRQGMAPYRDLVLAVSALDKPVIAAVDGVAYGAGLSLAL
CCCCCCCEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHH
LADIVLCSYRARMAAVFHRIGLVPDVGAWYTLPRVVGLQRAKELVFSAREFGSDEAKRMG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LAMEVLAPEALMTRAIEIARSFESASGTAMSLSKQALQKSLQCDLETMLDLEATGQAIAA
HHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEC
GSDYTRESIRRFAAKESPQFRWAPLPATEYKND
CCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MNDSDDVKLTYDGGIALVTLNRPQAKNALTPAMTVALTEMFRSFRSDEQVRVVVFAGAGA
CCCCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
DFCSGGDVKAMGGGAPRTTEQRRQGMAPYRDLVLAVSALDKPVIAAVDGVAYGAGLSLAL
CCCCCCCEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHH
LADIVLCSYRARMAAVFHRIGLVPDVGAWYTLPRVVGLQRAKELVFSAREFGSDEAKRMG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LAMEVLAPEALMTRAIEIARSFESASGTAMSLSKQALQKSLQCDLETMLDLEATGQAIAA
HHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEC
GSDYTRESIRRFAAKESPQFRWAPLPATEYKND
CCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]