| Definition | Cupriavidus metallidurans CH34 megaplasmid, complete sequence. |
|---|---|
| Accession | NC_007974 |
| Length | 2,580,084 |
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The map label for this gene is cpnA [H]
Identifier: 94314433
GI number: 94314433
Start: 2260028
End: 2260822
Strand: Reverse
Name: cpnA [H]
Synonym: Rmet_5514
Alternate gene names: 94314433
Gene position: 2260822-2260028 (Counterclockwise)
Preceding gene: 94314434
Following gene: 291481581
Centisome position: 87.63
GC content: 58.99
Gene sequence:
>795_bases ATGCAACTTGAGAAAAAAGTCGTTTTGGTAACGGGTGCGGCCAGTGGAATCGGACGTCAGACTTGCGTCACGTTGGCAGC TGCTGGGGCGCGTGTCTTCGCAAGTGATCTCAATGAAGAGGGTGCCGCGCAGACCGTTGCGCTCATTCGCGACGCTGGCG GTGTCGCCGACAGCCTGGCGCATGATGTCACTGAGGAGAGTGCGTGGCATGCAGTCGTGCAGAAAATTGAGTCGGCGTCT GGCCGGCTCGATGGCCTGGTCAATAGCGCAGGGCTAATGGTTGTCCGACCATTCCTGGAGACCGACCTAGCATTGTATCG CCGTCAGCAGGCCGTCAACGTTGACAGCGTGTGGCTCGGGTGCCAAGCAGTCTGCAAGTTGATGACGAAAACGGCGGCCG AATTTGGGAGCGCCTCCATCGTGAATCTCTCTTCTATCCTTGGCCTGAAAGGTGGAAGCATGCACTCGGCGTACTGCACG TCGAAAGGTGCAGTTCGCTTGCTTACGAAGGCACTCGCCGTCGAGCTCGGAAGATCGCGGATTCGGGTCAATTCGGTCCA CCCGGGGCTAGTTGAGACCCCGCTTGGGTTAGGGTCCATGAAGGACATGATTAGCCACGGGGTCCCGCTGGGTTCTCTGG ACGCCGCCGTCGACATGGTTGCACACCGCACGCCTCTAGGTCGATGGGCACAACCCGTTGACGTGGCGAATGTCATCGCG TTCCTTTGTGCAGACGAGTCTCGATTCGTCACTGGCACTGAGCTTACTGTCGACGGAGGCTACAGCGCTGCCTAG
Upstream 100 bases:
>100_bases TGGGGAAGAGGGCCTGGCGAGCTATACGTTACCGCAAACGCTATCAGTGAAGCGTGCCGCGTGAATTCACCGTCTTTGAC AGCCTGAGGAAGAGGAATCT
Downstream 100 bases:
>100_bases TTGGGGTCCACCGAAGCTTGGCGCCGAAATTGATTCGGCGTCAGGCCTGTTGCTCGGCGAAAAGCGCGGTGAAAGGCGCT GGGCTCTGAGAATCCCAGGC
Product: Cyclopentanol dehydrogenase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 264; Mature: 264
Protein sequence:
>264_residues MQLEKKVVLVTGAASGIGRQTCVTLAAAGARVFASDLNEEGAAQTVALIRDAGGVADSLAHDVTEESAWHAVVQKIESAS GRLDGLVNSAGLMVVRPFLETDLALYRRQQAVNVDSVWLGCQAVCKLMTKTAAEFGSASIVNLSSILGLKGGSMHSAYCT SKGAVRLLTKALAVELGRSRIRVNSVHPGLVETPLGLGSMKDMISHGVPLGSLDAAVDMVAHRTPLGRWAQPVDVANVIA FLCADESRFVTGTELTVDGGYSAA
Sequences:
>Translated_264_residues MQLEKKVVLVTGAASGIGRQTCVTLAAAGARVFASDLNEEGAAQTVALIRDAGGVADSLAHDVTEESAWHAVVQKIESAS GRLDGLVNSAGLMVVRPFLETDLALYRRQQAVNVDSVWLGCQAVCKLMTKTAAEFGSASIVNLSSILGLKGGSMHSAYCT SKGAVRLLTKALAVELGRSRIRVNSVHPGLVETPLGLGSMKDMISHGVPLGSLDAAVDMVAHRTPLGRWAQPVDVANVIA FLCADESRFVTGTELTVDGGYSAA >Mature_264_residues MQLEKKVVLVTGAASGIGRQTCVTLAAAGARVFASDLNEEGAAQTVALIRDAGGVADSLAHDVTEESAWHAVVQKIESAS GRLDGLVNSAGLMVVRPFLETDLALYRRQQAVNVDSVWLGCQAVCKLMTKTAAEFGSASIVNLSSILGLKGGSMHSAYCT SKGAVRLLTKALAVELGRSRIRVNSVHPGLVETPLGLGSMKDMISHGVPLGSLDAAVDMVAHRTPLGRWAQPVDVANVIA FLCADESRFVTGTELTVDGGYSAA
Specific function: Reduces cyclopentanol to cyclopentanone. The enzyme also shows considerable activity toward cyclohexanol [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI7705925, Length=261, Percent_Identity=34.8659003831418, Blast_Score=125, Evalue=3e-29, Organism=Homo sapiens, GI15277342, Length=270, Percent_Identity=34.0740740740741, Blast_Score=122, Evalue=4e-28, Organism=Homo sapiens, GI40254992, Length=259, Percent_Identity=33.5907335907336, Blast_Score=114, Evalue=1e-25, Organism=Homo sapiens, GI66933014, Length=262, Percent_Identity=31.2977099236641, Blast_Score=92, Evalue=6e-19, Organism=Homo sapiens, GI32483357, Length=264, Percent_Identity=30.3030303030303, Blast_Score=85, Evalue=5e-17, Organism=Homo sapiens, GI59889578, Length=235, Percent_Identity=33.6170212765957, Blast_Score=83, Evalue=3e-16, Organism=Homo sapiens, GI31542939, Length=251, Percent_Identity=30.2788844621514, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI126723750, Length=263, Percent_Identity=27.3764258555133, Blast_Score=73, Evalue=2e-13, Organism=Homo sapiens, GI10190704, Length=268, Percent_Identity=28.3582089552239, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI5031737, Length=262, Percent_Identity=29.0076335877863, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI210032110, Length=191, Percent_Identity=27.2251308900524, Blast_Score=66, Evalue=4e-11, Organism=Homo sapiens, GI166795268, Length=209, Percent_Identity=31.5789473684211, Blast_Score=65, Evalue=4e-11, Organism=Escherichia coli, GI1790717, Length=260, Percent_Identity=32.3076923076923, Blast_Score=122, Evalue=2e-29, Organism=Escherichia coli, GI1787905, Length=260, Percent_Identity=33.4615384615385, Blast_Score=114, Evalue=6e-27, Organism=Escherichia coli, GI1787335, Length=260, Percent_Identity=31.9230769230769, Blast_Score=108, Evalue=3e-25, Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=31.1538461538462, Blast_Score=107, Evalue=7e-25, Organism=Escherichia coli, GI1788459, Length=259, Percent_Identity=30.5019305019305, Blast_Score=106, Evalue=2e-24, Organism=Escherichia coli, GI2367175, Length=260, Percent_Identity=34.6153846153846, Blast_Score=101, Evalue=4e-23, Organism=Escherichia coli, GI87082160, Length=261, Percent_Identity=27.9693486590038, Blast_Score=98, Evalue=7e-22, Organism=Escherichia coli, GI1789208, Length=263, Percent_Identity=28.5171102661597, Blast_Score=97, Evalue=9e-22, Organism=Escherichia coli, GI1789378, Length=265, Percent_Identity=30.188679245283, Blast_Score=87, Evalue=8e-19, Organism=Escherichia coli, GI1787526, Length=265, Percent_Identity=29.811320754717, Blast_Score=80, Evalue=1e-16, Organism=Escherichia coli, GI1786812, Length=263, Percent_Identity=27.7566539923954, Blast_Score=75, Evalue=6e-15, Organism=Escherichia coli, GI1789057, Length=260, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=8e-15, Organism=Escherichia coli, GI2367365, Length=262, Percent_Identity=28.6259541984733, Blast_Score=67, Evalue=2e-12, Organism=Caenorhabditis elegans, GI115534694, Length=257, Percent_Identity=34.2412451361868, Blast_Score=123, Evalue=8e-29, Organism=Caenorhabditis elegans, GI17555706, Length=258, Percent_Identity=33.7209302325581, Blast_Score=119, Evalue=1e-27, Organism=Caenorhabditis elegans, GI17562906, Length=267, Percent_Identity=33.7078651685393, Blast_Score=108, Evalue=2e-24, Organism=Caenorhabditis elegans, GI17562908, Length=273, Percent_Identity=32.967032967033, Blast_Score=108, Evalue=3e-24, Organism=Caenorhabditis elegans, GI17560150, Length=266, Percent_Identity=32.3308270676692, Blast_Score=108, Evalue=4e-24, Organism=Caenorhabditis elegans, GI71994600, Length=258, Percent_Identity=31.3953488372093, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI193204405, Length=278, Percent_Identity=33.4532374100719, Blast_Score=103, Evalue=1e-22, Organism=Caenorhabditis elegans, GI71994604, Length=261, Percent_Identity=33.7164750957854, Blast_Score=103, Evalue=1e-22, Organism=Caenorhabditis elegans, GI17531453, Length=275, Percent_Identity=33.0909090909091, Blast_Score=102, Evalue=2e-22, Organism=Caenorhabditis elegans, GI17563726, Length=268, Percent_Identity=30.9701492537313, Blast_Score=102, Evalue=2e-22, Organism=Caenorhabditis elegans, GI17562990, Length=266, Percent_Identity=31.203007518797, Blast_Score=101, Evalue=3e-22, Organism=Caenorhabditis elegans, GI17551412, Length=275, Percent_Identity=30.1818181818182, Blast_Score=101, Evalue=4e-22, Organism=Caenorhabditis elegans, GI17562910, Length=278, Percent_Identity=30.5755395683453, Blast_Score=100, Evalue=1e-21, Organism=Caenorhabditis elegans, GI25147288, Length=257, Percent_Identity=31.1284046692607, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17562904, Length=276, Percent_Identity=29.7101449275362, Blast_Score=98, Evalue=5e-21, Organism=Caenorhabditis elegans, GI17559104, Length=272, Percent_Identity=32.7205882352941, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI17538480, Length=272, Percent_Identity=30.5147058823529, Blast_Score=96, Evalue=3e-20, Organism=Caenorhabditis elegans, GI17560220, Length=270, Percent_Identity=29.6296296296296, Blast_Score=95, Evalue=4e-20, Organism=Caenorhabditis elegans, GI17560332, Length=273, Percent_Identity=31.1355311355311, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI72000259, Length=271, Percent_Identity=30.6273062730627, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17544670, Length=271, Percent_Identity=29.520295202952, Blast_Score=85, Evalue=4e-17, Organism=Caenorhabditis elegans, GI17536025, Length=261, Percent_Identity=26.8199233716475, Blast_Score=84, Evalue=8e-17, Organism=Caenorhabditis elegans, GI17538182, Length=262, Percent_Identity=28.6259541984733, Blast_Score=82, Evalue=2e-16, Organism=Caenorhabditis elegans, GI17561402, Length=272, Percent_Identity=27.5735294117647, Blast_Score=79, Evalue=2e-15, Organism=Caenorhabditis elegans, GI17538486, Length=275, Percent_Identity=29.0909090909091, Blast_Score=79, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17564282, Length=194, Percent_Identity=28.8659793814433, Blast_Score=79, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17567345, Length=195, Percent_Identity=28.2051282051282, Blast_Score=78, Evalue=4e-15, Organism=Caenorhabditis elegans, GI17565030, Length=280, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-14, Organism=Caenorhabditis elegans, GI17536651, Length=262, Percent_Identity=25.9541984732824, Blast_Score=75, Evalue=3e-14, Organism=Caenorhabditis elegans, GI17560676, Length=270, Percent_Identity=28.5185185185185, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI193203117, Length=195, Percent_Identity=25.1282051282051, Blast_Score=67, Evalue=1e-11, Organism=Caenorhabditis elegans, GI193203115, Length=190, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=2e-11, Organism=Saccharomyces cerevisiae, GI6322227, Length=202, Percent_Identity=30.1980198019802, Blast_Score=65, Evalue=1e-11, Organism=Saccharomyces cerevisiae, GI6322861, Length=197, Percent_Identity=26.9035532994924, Blast_Score=63, Evalue=5e-11, Organism=Drosophila melanogaster, GI24643142, Length=262, Percent_Identity=35.1145038167939, Blast_Score=122, Evalue=2e-28, Organism=Drosophila melanogaster, GI24639444, Length=259, Percent_Identity=35.9073359073359, Blast_Score=118, Evalue=3e-27, Organism=Drosophila melanogaster, GI21357041, Length=265, Percent_Identity=35.0943396226415, Blast_Score=117, Evalue=9e-27, Organism=Drosophila melanogaster, GI28571526, Length=262, Percent_Identity=33.969465648855, Blast_Score=111, Evalue=6e-25, Organism=Drosophila melanogaster, GI23397609, Length=262, Percent_Identity=32.0610687022901, Blast_Score=101, Evalue=5e-22, Organism=Drosophila melanogaster, GI24644339, Length=272, Percent_Identity=33.0882352941176, Blast_Score=97, Evalue=9e-21, Organism=Drosophila melanogaster, GI24644337, Length=259, Percent_Identity=33.2046332046332, Blast_Score=95, Evalue=4e-20, Organism=Drosophila melanogaster, GI24640492, Length=194, Percent_Identity=32.9896907216495, Blast_Score=91, Evalue=9e-19, Organism=Drosophila melanogaster, GI21355319, Length=263, Percent_Identity=28.1368821292776, Blast_Score=82, Evalue=3e-16, Organism=Drosophila melanogaster, GI281364892, Length=236, Percent_Identity=32.2033898305085, Blast_Score=79, Evalue=3e-15, Organism=Drosophila melanogaster, GI17737361, Length=268, Percent_Identity=27.6119402985075, Blast_Score=70, Evalue=2e-12, Organism=Drosophila melanogaster, GI21358495, Length=203, Percent_Identity=27.5862068965517, Blast_Score=66, Evalue=2e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: =1.1.1.163 [H]
Molecular weight: Translated: 27472; Mature: 27472
Theoretical pI: Translated: 6.78; Mature: 6.78
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.9 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 4.5 %Cys+Met (Translated Protein) 1.9 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 4.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQLEKKVVLVTGAASGIGRQTCVTLAAAGARVFASDLNEEGAAQTVALIRDAGGVADSLA CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHH HDVTEESAWHAVVQKIESASGRLDGLVNSAGLMVVRPFLETDLALYRRQQAVNVDSVWLG HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH CQAVCKLMTKTAAEFGSASIVNLSSILGLKGGSMHSAYCTSKGAVRLLTKALAVELGRSR HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCC IRVNSVHPGLVETPLGLGSMKDMISHGVPLGSLDAAVDMVAHRTPLGRWAQPVDVANVIA EEEECCCCCHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHH FLCADESRFVTGTELTVDGGYSAA HHHCCCCCEEECCEEEECCCCCCC >Mature Secondary Structure MQLEKKVVLVTGAASGIGRQTCVTLAAAGARVFASDLNEEGAAQTVALIRDAGGVADSLA CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHH HDVTEESAWHAVVQKIESASGRLDGLVNSAGLMVVRPFLETDLALYRRQQAVNVDSVWLG HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH CQAVCKLMTKTAAEFGSASIVNLSSILGLKGGSMHSAYCTSKGAVRLLTKALAVELGRSR HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCC IRVNSVHPGLVETPLGLGSMKDMISHGVPLGSLDAAVDMVAHRTPLGRWAQPVDVANVIA EEEECCCCCHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHH FLCADESRFVTGTELTVDGGYSAA HHHCCCCCEEECCEEEECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 12406764 [H]