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The map label for this gene is fadJ [H]

Identifier: 94314430

GI number: 94314430

Start: 2255928

End: 2258072

Strand: Direct

Name: fadJ [H]

Synonym: Rmet_5511

Alternate gene names: 94314430

Gene position: 2255928-2258072 (Clockwise)

Preceding gene: 94314429

Following gene: 94314431

Centisome position: 87.44

GC content: 57.76

Gene sequence:

>2145_bases
ATGAGCTCAATCCAATATCAGAAAGACCAAGACGGCATTGTCACGCTGACGGTCGATATGCCCGGCCAGTCGGCCAACAC
GATGAACCAGGCATTCCGAACCGACTTCTCTGCGGTCGCGTCTCAGCTTGAGTCCGAGCAAGACGGTATTACTGGAGTCA
TCCTCACCTCCGGAAAGAAGACATTTTTTGCCGGTGGCGACCTGAATGGACTTCTCGCTGTAACGCCTGAACAAAAGGAG
GAACTCTTCAAGCGTGCCACCGAGCTGAAGGCAGCGATGCGGCGCATTGAGCTTCTTGGAAAGCCCGTTGTTGCCGCAAT
CAATGGCAGCGCGCTAGGGGGCGGCTTCGAACTTTGCCTCGCCTGCCACGCACGTTTTTCACTTGCCAGTCCGCAAATCG
CGTTGGGCCTCCCTGAGGTAAATCTCGGACTCCTTCCCGGCGGCGGTGGCGTCGTGCGACTCGTGCGATACCTAGGTCTC
GAAGCTGCAATGCCTCTCCTTCTTGAGGGCACCTCCCTGTCGCCTGCCCAAGCGCTAGCGAAGGGCCTTCTTACCAGCAT
CGCGGACGATGCGGCTGGTCTGGTAAAGATGGCCCGAGATTGGATCAAGGAAAATCCTGAGGCCGCTCAGCCTTGGGACA
AGAAGGGGTGGGGCCTGCCAGGCTCGACTGAGCAGTCTCCGGCATCTTTGACGTTCCTGCGAACCGCACCGGTGGCTCTT
ATGAAGAAGACCCGCGGTTGCTACCCTGCCCCACTGGCAATCCTCTCCGCTGCTGTAGAGGGTGCAGTCGTCAACTTCGA
CACAGCATCCCTTATCGAAACTCGCTACTTCGTTGGATTGGCGACGGGTCCTGTAGCCAAGAACCTGATCAGCACATTCT
TCTTCCAGATGAATGACATCAAGGCCAACAAGGGCCGACCGGCAGATGTTCCTCCTGCCACGTTCAAGCGCGTCGGGATC
CTGGGAGCAGGCATGATGGGCGCGGGCATTGCATATGCCAGCGCTATGCGCGGAATCGAGGCCGTTCTCAAGGACGTTAG
CCTTGACCACGCGGGCAAAGGAAAGCTCCACTCAGAGAAGCTCCTTGAGAAAGGAGTGAGTAAGGGGAAGATCTCTCCAT
CGAAGCGAGACGAAGTCCTGCAACGAATTACCCCTACGGCAGATGCTTCCGGCCTCGCCGGCTGCGACATCATCATCGAA
GCGGTTTACGAAAAGCGTGAGCTGAAAGCCGAAGTCACCAGGGAAGCGGAGCCGCATCTCGCGGAAAACGGCCTGTTTGC
GTCAAATACGTCAACTCTGCCGATTACCGGCCTGGCCGAAGCAAGCGCAAGCCCAGAAAACTTCATCGGCCTGCACTTCT
TCTCTCCAGTGGATCGAATGCCTCTGGTGGAAATCATCAAGGGAAAGAAGACCTCCTCCCGAACATTGGCGCACGCTATC
GATTTCGTCAAACAGATTGGCAAGACGCCTATCGTCGTCAACGATTCTCGCGGCTTCTTTACCAGCAGGGTCTTCGGCAC
CTTCACCAAAGAAGGTGCGGCGATGCTCTCGGAAGGTGTCCCTGCCGCGGTAATCGAGAACGCGGCCCTTAGCGTCGGAA
TGCCGGTCGGTCCATTGGCGGTCATGGACGAGACCTCAATGGCCCTCAGCTTGTCGGTAAAGCGGCAGACCGAAACGGAT
CTTGCCTCAGAAGGTAAAAGCATGCCGCAACATCCGGGATGGGCGGTCATCGAACGCATGGCCGATGGACTGAAGCGTCC
TGGCCGAGCCGGCGGTGGCGGCTTCTACGAGTATCCGAAGGATGGCCAGAAGTACTTGTGGCCTGGTTTGGTGAGCGAAT
TCGGAAAGTCGGCGGGGGATGTTCCGTTGGATGACCTGCGTGATCGCATCCTCTACATTCAGGCGTTGGAATCCATCCGG
ATCCTTGAAGAGGGGGTTATCGATACTACGCGCGACGCGAACATCGGCTCGATCCTCGGCATAGGCTTTCCTCGCTGGAC
TGGAGGTGTGCTGCAGTACGTGAACATGGTCGGTACCCGTCGATTCGCAGAGCGCTCCGCAGAACTGGCACAGCGATACG
GTGAACGGTTTACCCCCCCGGCCCTCCTTCTGGAGAAGGCCGAACGCAACGAACGCTTCCAGTAA

Upstream 100 bases:

>100_bases
ACGAACTCGAACGCCGCAACCTCAAGCGCGGCTTGATCACACTGTGCGTCGCCGGCGGTATGGGAATCGCCACCATCATC
GAACGCGTCTGAGAGAGAAT

Downstream 100 bases:

>100_bases
CGAAGGATCACCCATGACTTGGGATAGTTTTACGTTATCGGTGGAAGACCACGTCGCCCACCTTGTGCTGAATCGGCCCG
AGAAGCGAAATGCTTTGTCT

Product: 3-hydroxybutyryl-CoA epimerase

Products: NA

Alternate protein names: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MSSIQYQKDQDGIVTLTVDMPGQSANTMNQAFRTDFSAVASQLESEQDGITGVILTSGKKTFFAGGDLNGLLAVTPEQKE
ELFKRATELKAAMRRIELLGKPVVAAINGSALGGGFELCLACHARFSLASPQIALGLPEVNLGLLPGGGGVVRLVRYLGL
EAAMPLLLEGTSLSPAQALAKGLLTSIADDAAGLVKMARDWIKENPEAAQPWDKKGWGLPGSTEQSPASLTFLRTAPVAL
MKKTRGCYPAPLAILSAAVEGAVVNFDTASLIETRYFVGLATGPVAKNLISTFFFQMNDIKANKGRPADVPPATFKRVGI
LGAGMMGAGIAYASAMRGIEAVLKDVSLDHAGKGKLHSEKLLEKGVSKGKISPSKRDEVLQRITPTADASGLAGCDIIIE
AVYEKRELKAEVTREAEPHLAENGLFASNTSTLPITGLAEASASPENFIGLHFFSPVDRMPLVEIIKGKKTSSRTLAHAI
DFVKQIGKTPIVVNDSRGFFTSRVFGTFTKEGAAMLSEGVPAAVIENAALSVGMPVGPLAVMDETSMALSLSVKRQTETD
LASEGKSMPQHPGWAVIERMADGLKRPGRAGGGGFYEYPKDGQKYLWPGLVSEFGKSAGDVPLDDLRDRILYIQALESIR
ILEEGVIDTTRDANIGSILGIGFPRWTGGVLQYVNMVGTRRFAERSAELAQRYGERFTPPALLLEKAERNERFQ

Sequences:

>Translated_714_residues
MSSIQYQKDQDGIVTLTVDMPGQSANTMNQAFRTDFSAVASQLESEQDGITGVILTSGKKTFFAGGDLNGLLAVTPEQKE
ELFKRATELKAAMRRIELLGKPVVAAINGSALGGGFELCLACHARFSLASPQIALGLPEVNLGLLPGGGGVVRLVRYLGL
EAAMPLLLEGTSLSPAQALAKGLLTSIADDAAGLVKMARDWIKENPEAAQPWDKKGWGLPGSTEQSPASLTFLRTAPVAL
MKKTRGCYPAPLAILSAAVEGAVVNFDTASLIETRYFVGLATGPVAKNLISTFFFQMNDIKANKGRPADVPPATFKRVGI
LGAGMMGAGIAYASAMRGIEAVLKDVSLDHAGKGKLHSEKLLEKGVSKGKISPSKRDEVLQRITPTADASGLAGCDIIIE
AVYEKRELKAEVTREAEPHLAENGLFASNTSTLPITGLAEASASPENFIGLHFFSPVDRMPLVEIIKGKKTSSRTLAHAI
DFVKQIGKTPIVVNDSRGFFTSRVFGTFTKEGAAMLSEGVPAAVIENAALSVGMPVGPLAVMDETSMALSLSVKRQTETD
LASEGKSMPQHPGWAVIERMADGLKRPGRAGGGGFYEYPKDGQKYLWPGLVSEFGKSAGDVPLDDLRDRILYIQALESIR
ILEEGVIDTTRDANIGSILGIGFPRWTGGVLQYVNMVGTRRFAERSAELAQRYGERFTPPALLLEKAERNERFQ
>Mature_713_residues
SSIQYQKDQDGIVTLTVDMPGQSANTMNQAFRTDFSAVASQLESEQDGITGVILTSGKKTFFAGGDLNGLLAVTPEQKEE
LFKRATELKAAMRRIELLGKPVVAAINGSALGGGFELCLACHARFSLASPQIALGLPEVNLGLLPGGGGVVRLVRYLGLE
AAMPLLLEGTSLSPAQALAKGLLTSIADDAAGLVKMARDWIKENPEAAQPWDKKGWGLPGSTEQSPASLTFLRTAPVALM
KKTRGCYPAPLAILSAAVEGAVVNFDTASLIETRYFVGLATGPVAKNLISTFFFQMNDIKANKGRPADVPPATFKRVGIL
GAGMMGAGIAYASAMRGIEAVLKDVSLDHAGKGKLHSEKLLEKGVSKGKISPSKRDEVLQRITPTADASGLAGCDIIIEA
VYEKRELKAEVTREAEPHLAENGLFASNTSTLPITGLAEASASPENFIGLHFFSPVDRMPLVEIIKGKKTSSRTLAHAID
FVKQIGKTPIVVNDSRGFFTSRVFGTFTKEGAAMLSEGVPAAVIENAALSVGMPVGPLAVMDETSMALSLSVKRQTETDL
ASEGKSMPQHPGWAVIERMADGLKRPGRAGGGGFYEYPKDGQKYLWPGLVSEFGKSAGDVPLDDLRDRILYIQALESIRI
LEEGVIDTTRDANIGSILGIGFPRWTGGVLQYVNMVGTRRFAERSAELAQRYGERFTPPALLLEKAERNERFQ

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI20127408, Length=726, Percent_Identity=31.9559228650138, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI68989263, Length=657, Percent_Identity=27.8538812785388, Blast_Score=193, Evalue=4e-49,
Organism=Homo sapiens, GI261878539, Length=596, Percent_Identity=27.6845637583893, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI296179429, Length=294, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI296179427, Length=311, Percent_Identity=31.5112540192926, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI194097323, Length=165, Percent_Identity=31.5151515151515, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI157694516, Length=205, Percent_Identity=28.780487804878, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI213417737, Length=205, Percent_Identity=28.780487804878, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788682, Length=713, Percent_Identity=36.1851332398317, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1790281, Length=713, Percent_Identity=32.6788218793829, Blast_Score=317, Evalue=2e-87,
Organism=Escherichia coli, GI1787661, Length=285, Percent_Identity=29.1228070175439, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1787659, Length=185, Percent_Identity=31.3513513513514, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI221142681, Length=200, Percent_Identity=30.5, Blast_Score=73, Evalue=8e-14,
Organism=Escherichia coli, GI1788597, Length=215, Percent_Identity=26.046511627907, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=744, Percent_Identity=31.3172043010753, Blast_Score=300, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17508953, Length=758, Percent_Identity=31.3984168865435, Blast_Score=299, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17558304, Length=746, Percent_Identity=30.4289544235925, Blast_Score=287, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI25144276, Length=627, Percent_Identity=32.5358851674641, Blast_Score=266, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI71985923, Length=380, Percent_Identity=27.6315789473684, Blast_Score=152, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI71985930, Length=380, Percent_Identity=26.8421052631579, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17553560, Length=295, Percent_Identity=31.1864406779661, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17549919, Length=296, Percent_Identity=28.3783783783784, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17563036, Length=304, Percent_Identity=28.2894736842105, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=202, Percent_Identity=29.7029702970297, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=209, Percent_Identity=25.8373205741627, Blast_Score=73, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=177, Percent_Identity=28.2485875706215, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=220, Percent_Identity=30, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19921000, Length=732, Percent_Identity=32.103825136612, Blast_Score=298, Evalue=9e-81,
Organism=Drosophila melanogaster, GI24583077, Length=732, Percent_Identity=32.103825136612, Blast_Score=298, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24583079, Length=732, Percent_Identity=32.103825136612, Blast_Score=298, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24653139, Length=178, Percent_Identity=31.4606741573034, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI20129971, Length=178, Percent_Identity=30.8988764044944, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653477, Length=178, Percent_Identity=30.8988764044944, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR012802
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =1.1.1.35 [H]

Molecular weight: Translated: 76208; Mature: 76076

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIQYQKDQDGIVTLTVDMPGQSANTMNQAFRTDFSAVASQLESEQDGITGVILTSGKK
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCE
TFFAGGDLNGLLAVTPEQKEELFKRATELKAAMRRIELLGKPVVAAINGSALGGGFELCL
EEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
ACHARFSLASPQIALGLPEVNLGLLPGGGGVVRLVRYLGLEAAMPLLLEGTSLSPAQALA
HHHHHHCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCHHEEECCCCCHHHHHH
KGLLTSIADDAAGLVKMARDWIKENPEAAQPWDKKGWGLPGSTEQSPASLTFLRTAPVAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHH
MKKTRGCYPAPLAILSAAVEGAVVNFDTASLIETRYFVGLATGPVAKNLISTFFFQMNDI
HHHHCCCCCHHHHHHHHHHCCCEEECCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCC
KANKGRPADVPPATFKRVGILGAGMMGAGIAYASAMRGIEAVLKDVSLDHAGKGKLHSEK
CCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LLEKGVSKGKISPSKRDEVLQRITPTADASGLAGCDIIIEAVYEKRELKAEVTREAEPHL
HHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
AENGLFASNTSTLPITGLAEASASPENFIGLHFFSPVDRMPLVEIIKGKKTSSRTLAHAI
HCCCEEECCCCCCCEECCHHCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCHHHHHHHH
DFVKQIGKTPIVVNDSRGFFTSRVFGTFTKEGAAMLSEGVPAAVIENAALSVGMPVGPLA
HHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHCCCCCCCCEE
VMDETSMALSLSVKRQTETDLASEGKSMPQHPGWAVIERMADGLKRPGRAGGGGFYEYPK
EECCCCEEEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
DGQKYLWPGLVSEFGKSAGDVPLDDLRDRILYIQALESIRILEEGVIDTTRDANIGSILG
CCCCEECHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
IGFPRWTGGVLQYVNMVGTRRFAERSAELAQRYGERFTPPALLLEKAERNERFQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSIQYQKDQDGIVTLTVDMPGQSANTMNQAFRTDFSAVASQLESEQDGITGVILTSGKK
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCE
TFFAGGDLNGLLAVTPEQKEELFKRATELKAAMRRIELLGKPVVAAINGSALGGGFELCL
EEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
ACHARFSLASPQIALGLPEVNLGLLPGGGGVVRLVRYLGLEAAMPLLLEGTSLSPAQALA
HHHHHHCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCHHEEECCCCCHHHHHH
KGLLTSIADDAAGLVKMARDWIKENPEAAQPWDKKGWGLPGSTEQSPASLTFLRTAPVAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHH
MKKTRGCYPAPLAILSAAVEGAVVNFDTASLIETRYFVGLATGPVAKNLISTFFFQMNDI
HHHHCCCCCHHHHHHHHHHCCCEEECCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCC
KANKGRPADVPPATFKRVGILGAGMMGAGIAYASAMRGIEAVLKDVSLDHAGKGKLHSEK
CCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
LLEKGVSKGKISPSKRDEVLQRITPTADASGLAGCDIIIEAVYEKRELKAEVTREAEPHL
HHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
AENGLFASNTSTLPITGLAEASASPENFIGLHFFSPVDRMPLVEIIKGKKTSSRTLAHAI
HCCCEEECCCCCCCEECCHHCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCHHHHHHHH
DFVKQIGKTPIVVNDSRGFFTSRVFGTFTKEGAAMLSEGVPAAVIENAALSVGMPVGPLA
HHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHCCCCCCCCEE
VMDETSMALSLSVKRQTETDLASEGKSMPQHPGWAVIERMADGLKRPGRAGGGGFYEYPK
EECCCCEEEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
DGQKYLWPGLVSEFGKSAGDVPLDDLRDRILYIQALESIRILEEGVIDTTRDANIGSILG
CCCCEECHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
IGFPRWTGGVLQYVNMVGTRRFAERSAELAQRYGERFTPPALLLEKAERNERFQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA