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The map label for this gene is fadA [H]

Identifier: 94314429

GI number: 94314429

Start: 2254711

End: 2255919

Strand: Direct

Name: fadA [H]

Synonym: Rmet_5510

Alternate gene names: 94314429

Gene position: 2254711-2255919 (Clockwise)

Preceding gene: 94314428

Following gene: 94314430

Centisome position: 87.39

GC content: 59.39

Gene sequence:

>1209_bases
ATGAACGAGGCCTACATCTTTGATGCATTGCGCACCCCCCGCGGCAGAGGACGTGCCGATGGTAGTCTCTACGAAGTCAA
CCCAGTCGACCTCCTCGCTGGACTTATGACCGAGCTCCAGCAGCGACATGATCTCGACACTGCCCAAGTTGAGGATGCGC
TCATCGGCTGTGTAATGCCCAACGGTGAGCAAAGCGGATGTATCGCCAAAACGGCGGCTTTGCGTGCTAACTGGGATTGG
CAGGTACCAGGCGTACAGCTTGACCGATACTGCGGCTCTGGCTTGGAGACAGTGAATATCGCTGCCGCAAAGGTCCGCTC
TGGCTGGGAGAATCTTCTTGTCGCTGGCGGCGTCGAGTCCATGTCGCGTATCACGATGGGTGGTGCGCCTGGTCCTCGGG
ACACAAAACCTGACATGGTCTTTCAGCTTAAACCAGTGCCGACTGGCGTGGCTGCGGACCTCATGGCTACTTTGGACGGA
CACACGCGCGAAGCTGTCGATCGTTACGCTTTGCAATCCCAGCAGCGTGCCGGCCATGCCCGTGCCAGCGGATACTTCGA
CCGCTCTGTTGTACCTGTCAAGGATGCCAACGGTCTCACTATTCTGAAACAGGATGAGCTAATCCGCGAAGACACTACTC
TCGAGAAGCTTGGCGCACTCAAGGCCTCCTTCCAGGCGATGGGCGAACTCGGAGGTTTTGACGCGCTGGCACAGATGAAG
TACCCGGAAGTCGAAAAGGTGCGTCACATCCATACCGGCGGGAACTCCTCGGGCATTGTCGATGGCGCCTCACTGTTGCT
CGTCGGGTCAGAGCAAAAGGGGCGCGAGTTGGGCCTCACGCCACGAGCGCGCGTGGTTTCTGTTGCAGCAGTCGGCGCCG
AGCCGACCATCATGCTTGCCGGCCCGGCGCCGGCGAGTCGCCTGGCATTGAAAAAGGCCGGCCTTAGCATCGACGACATC
GACCTTATCGAGGTGAATGAAGCGTTCGCGTCAGTCGTTCTGCGGTTCATGACTGAAATGCAGGTTGACCCAGCCAAGGT
CAACGTGAATGGTGGCGCGATTGCTATGGGACACCCCCTCGGTGCGACCGGCGGCATGCTGCTCGGCACACTCCTTGACG
AACTCGAACGCCGCAACCTCAAGCGCGGCTTGATCACACTGTGCGTCGCCGGCGGTATGGGAATCGCCACCATCATCGAA
CGCGTCTGA

Upstream 100 bases:

>100_bases
GATTGCGACGGGGGAAGCGGCCTAGGGGACGCCTCCGCAGATGCGCTTCTTAAAGCAACTCAACGTTGAATATTTCGACC
ACGGTCGTGCAAGGAAAAAT

Downstream 100 bases:

>100_bases
GAGAGAATATGAGCTCAATCCAATATCAGAAAGACCAAGACGGCATTGTCACGCTGACGGTCGATATGCCCGGCCAGTCG
GCCAACACGATGAACCAGGC

Product: beta-ketoadipyl CoA thiolase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MNEAYIFDALRTPRGRGRADGSLYEVNPVDLLAGLMTELQQRHDLDTAQVEDALIGCVMPNGEQSGCIAKTAALRANWDW
QVPGVQLDRYCGSGLETVNIAAAKVRSGWENLLVAGGVESMSRITMGGAPGPRDTKPDMVFQLKPVPTGVAADLMATLDG
HTREAVDRYALQSQQRAGHARASGYFDRSVVPVKDANGLTILKQDELIREDTTLEKLGALKASFQAMGELGGFDALAQMK
YPEVEKVRHIHTGGNSSGIVDGASLLLVGSEQKGRELGLTPRARVVSVAAVGAEPTIMLAGPAPASRLALKKAGLSIDDI
DLIEVNEAFASVVLRFMTEMQVDPAKVNVNGGAIAMGHPLGATGGMLLGTLLDELERRNLKRGLITLCVAGGMGIATIIE
RV

Sequences:

>Translated_402_residues
MNEAYIFDALRTPRGRGRADGSLYEVNPVDLLAGLMTELQQRHDLDTAQVEDALIGCVMPNGEQSGCIAKTAALRANWDW
QVPGVQLDRYCGSGLETVNIAAAKVRSGWENLLVAGGVESMSRITMGGAPGPRDTKPDMVFQLKPVPTGVAADLMATLDG
HTREAVDRYALQSQQRAGHARASGYFDRSVVPVKDANGLTILKQDELIREDTTLEKLGALKASFQAMGELGGFDALAQMK
YPEVEKVRHIHTGGNSSGIVDGASLLLVGSEQKGRELGLTPRARVVSVAAVGAEPTIMLAGPAPASRLALKKAGLSIDDI
DLIEVNEAFASVVLRFMTEMQVDPAKVNVNGGAIAMGHPLGATGGMLLGTLLDELERRNLKRGLITLCVAGGMGIATIIE
RV
>Mature_402_residues
MNEAYIFDALRTPRGRGRADGSLYEVNPVDLLAGLMTELQQRHDLDTAQVEDALIGCVMPNGEQSGCIAKTAALRANWDW
QVPGVQLDRYCGSGLETVNIAAAKVRSGWENLLVAGGVESMSRITMGGAPGPRDTKPDMVFQLKPVPTGVAADLMATLDG
HTREAVDRYALQSQQRAGHARASGYFDRSVVPVKDANGLTILKQDELIREDTTLEKLGALKASFQAMGELGGFDALAQMK
YPEVEKVRHIHTGGNSSGIVDGASLLLVGSEQKGRELGLTPRARVVSVAAVGAEPTIMLAGPAPASRLALKKAGLSIDDI
DLIEVNEAFASVVLRFMTEMQVDPAKVNVNGGAIAMGHPLGATGGMLLGTLLDELERRNLKRGLITLCVAGGMGIATIIE
RV

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=34.4221105527638, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI167614485, Length=415, Percent_Identity=33.4939759036145, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI4501853, Length=392, Percent_Identity=34.9489795918367, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI4557237, Length=418, Percent_Identity=28.4688995215311, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI4504327, Length=443, Percent_Identity=28.2167042889391, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194353979, Length=115, Percent_Identity=41.7391304347826, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1787663, Length=424, Percent_Identity=41.0377358490566, Blast_Score=261, Evalue=4e-71,
Organism=Escherichia coli, GI48994986, Length=414, Percent_Identity=39.8550724637681, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1788554, Length=422, Percent_Identity=36.2559241706161, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI87082165, Length=415, Percent_Identity=33.9759036144578, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788683, Length=443, Percent_Identity=28.8939051918736, Blast_Score=118, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI133906874, Length=412, Percent_Identity=34.2233009708738, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17535921, Length=411, Percent_Identity=29.683698296837, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI25147385, Length=417, Percent_Identity=27.8177458033573, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17551802, Length=448, Percent_Identity=27.2321428571429, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17535917, Length=411, Percent_Identity=25.7907542579075, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6322031, Length=402, Percent_Identity=30.5970149253731, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6325229, Length=417, Percent_Identity=28.7769784172662, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24655093, Length=415, Percent_Identity=35.9036144578313, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI17648125, Length=413, Percent_Identity=32.6876513317191, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24640423, Length=410, Percent_Identity=28.0487804878049, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI17137578, Length=443, Percent_Identity=27.5395033860045, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42633; Mature: 42633

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEAYIFDALRTPRGRGRADGSLYEVNPVDLLAGLMTELQQRHDLDTAQVEDALIGCVMP
CCCEEEEHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHEEEECC
NGEQSGCIAKTAALRANWDWQVPGVQLDRYCGSGLETVNIAAAKVRSGWENLLVAGGVES
CCCCCCCEEHHHHHCCCCCCCCCCCCHHHHHCCCCCEEHHHHHHHHCCHHHEEEECCHHH
MSRITMGGAPGPRDTKPDMVFQLKPVPTGVAADLMATLDGHTREAVDRYALQSQQRAGHA
HHHEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCH
RASGYFDRSVVPVKDANGLTILKQDELIREDTTLEKLGALKASFQAMGELGGFDALAQMK
HHCCCCCCCEEEEECCCCEEEEECCHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHCC
YPEVEKVRHIHTGGNSSGIVDGASLLLVGSEQKGRELGLTPRARVVSVAAVGAEPTIMLA
CCCHHHHHEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHEEEEEEECCCCEEEEE
GPAPASRLALKKAGLSIDDIDLIEVNEAFASVVLRFMTEMQVDPAKVNVNGGAIAMGHPL
CCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCC
GATGGMLLGTLLDELERRNLKRGLITLCVAGGMGIATIIERV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MNEAYIFDALRTPRGRGRADGSLYEVNPVDLLAGLMTELQQRHDLDTAQVEDALIGCVMP
CCCEEEEHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHEEEECC
NGEQSGCIAKTAALRANWDWQVPGVQLDRYCGSGLETVNIAAAKVRSGWENLLVAGGVES
CCCCCCCEEHHHHHCCCCCCCCCCCCHHHHHCCCCCEEHHHHHHHHCCHHHEEEECCHHH
MSRITMGGAPGPRDTKPDMVFQLKPVPTGVAADLMATLDGHTREAVDRYALQSQQRAGHA
HHHEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCH
RASGYFDRSVVPVKDANGLTILKQDELIREDTTLEKLGALKASFQAMGELGGFDALAQMK
HHCCCCCCCEEEEECCCCEEEEECCHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHCC
YPEVEKVRHIHTGGNSSGIVDGASLLLVGSEQKGRELGLTPRARVVSVAAVGAEPTIMLA
CCCHHHHHEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHEEEEEEECCCCEEEEE
GPAPASRLALKKAGLSIDDIDLIEVNEAFASVVLRFMTEMQVDPAKVNVNGGAIAMGHPL
CCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCC
GATGGMLLGTLLDELERRNLKRGLITLCVAGGMGIATIIERV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]