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The map label for this gene is kstD [H]

Identifier: 94314411

GI number: 94314411

Start: 2237144

End: 2238847

Strand: Direct

Name: kstD [H]

Synonym: Rmet_5492

Alternate gene names: 94314411

Gene position: 2237144-2238847 (Clockwise)

Preceding gene: 94314410

Following gene: 94314412

Centisome position: 86.71

GC content: 57.51

Gene sequence:

>1704_bases
ATGTCTCAACAGCTCACTTACGATCTAGTCGTCATTGGTTCTGGCGCAGCGGGTCTCTCCTGCGCCATCACCGCGCGCAA
GAAGGGAATGAATGTCGTTGTCTTGGAGAAGGAACCCGTTTTCGGGGGTACAACCGCCCTATCGGGAGGTGTGCTTTGGG
TACCGCTGACTCATCATGGGTTGAAACAGAATCCCCAAGACTCCATTGAGGCCGTACAGGAGTTCCTTCGGAGCGAGACC
GGTCACTTCTACGATGAGGTAGTGGCGCGGGCGTTTATCGAAGCCGGTCCGGAGATGGTCAGCTTCTTCGAGCGAGAAAC
AGAGATGAAGTTCGTCCCGACTCTGTATCCCGACTACCATCCCATCGCGCCCGGGGGCGTAGACGTGGGTCGATCCATTC
TCGCCGCCCCATTTGATATCCGAAGCCTCGGTAAGGACATGCAGCGGCTCAAGCCACCATTGAAGACCATCACCTTCATG
GGGATGATGTTCAACTCGTCCAATGCCGATCTCAAGCACTTTTTCCGTGCCACGAAATCCTTCCGGTCCTTCCTGTATGT
GGCACGCCGGCTCGCCAATCATGTCAAGGAGATCGCTCTCTACCGGCGCGCGGTGCAGGTAACGAGCGGCAACGCGCTTG
CCGCTCGTCTTGCCAAATCGGCGTTGGACCTCAACATTCCGATCCTCACAAACGCTCCAGCAACCCGGCTCATCACCGAC
GGAGACCATGTTAAGGGCGTGGTAGCGAATACCGAGGACGGCGAAAGGGAGTTCCTCGCGCGTAATGGTGTGGTGCTTGC
ATGTGGGGGATTCCCACATGACCTCAATCGTATTGGTGCGGCCTACCCGCATCTGCGACGTGGCCATGAACACCTCTCCC
CGACTCCGAAGAGCAATACAGGAGACGGTGTGGCGCTCGCCGAGACTGCCGGCGGCCACGCTGACATTCGATACACGTCA
CCGGCAGCCTGGATGCCCGTGACCAAAGTCGACTTCGGAAATGGGGAAATCGGCGTGTTCCCCCACCTGTTGGATCGCTA
CAAACCGGGCGTAATTGGCGTACTTAGCAACGGCGCACGCTTTACCAATGAGTCGAACTCCTACCACGACGTAGGCTCGG
CGATGATCGAAGCGTGTGCGGGTCTCAAAGAGACAGCCATGTGGCTTATTTGCGACCGCGAGACCCTCGGCAAGTACGGT
CTCGGCTATGTCAAACCGTCGCCGATGCCCATCGGACGCTTCCTTCGAAACGGCTACCTGTACCAGGGCCGCACGCTTGA
TGAGCTTGCACACGCTACAGGCATTGATCCCGCTGGGCTAGCGGAAACCGTCCATGAGTACAACAAATCGGCCCAGTTTG
GGAATGATCCAGCGTTTGGTCGCGGAACGACTTCATTCAATCGCTTTCTGGGCGACCCCGACAACAAGCCCAACCCTTGC
GTTGCGCCGCTAAAAAAGGGACCTTTCTACGCCATCAAGCTTGTCATGGGAGACCTTGGTACCTTTGACGGCCTCGCGAC
TAGCGTGACAGGAGAGGTTCTCAGGAAGGACGGTACGCGTATCGACGGGCTCTACGCAGTAGGAAACGATCGCGCAAGCA
TGATGGGTGGTAACTATCCTGGCGCCGGAATTACGCACGGACCCAACATGACCTTTGCGTATCTGACCGCGAAGCACATT
GCCAAAGAGGCCGGAGCGGAATGA

Upstream 100 bases:

>100_bases
CGCCTGATGCTTTCGCTGCCATCCTGAAGAAGGAAACCGCGCAGTGGCAGGACATCATCACCAAGTACAAGATCGAAGCC
GAATAGTCTTTCACCCCGAT

Downstream 100 bases:

>100_bases
CGGCAATCGCACGCCCGCTCGTAGACCATCGGGTCTACACAATTGCGCTGCGGAAGATGCCTGAGTTCATGGAAGTATTC
AACAGACTGGCTATGCCGAT

Product: putative succinate dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSQQLTYDLVVIGSGAAGLSCAITARKKGMNVVVLEKEPVFGGTTALSGGVLWVPLTHHGLKQNPQDSIEAVQEFLRSET
GHFYDEVVARAFIEAGPEMVSFFERETEMKFVPTLYPDYHPIAPGGVDVGRSILAAPFDIRSLGKDMQRLKPPLKTITFM
GMMFNSSNADLKHFFRATKSFRSFLYVARRLANHVKEIALYRRAVQVTSGNALAARLAKSALDLNIPILTNAPATRLITD
GDHVKGVVANTEDGEREFLARNGVVLACGGFPHDLNRIGAAYPHLRRGHEHLSPTPKSNTGDGVALAETAGGHADIRYTS
PAAWMPVTKVDFGNGEIGVFPHLLDRYKPGVIGVLSNGARFTNESNSYHDVGSAMIEACAGLKETAMWLICDRETLGKYG
LGYVKPSPMPIGRFLRNGYLYQGRTLDELAHATGIDPAGLAETVHEYNKSAQFGNDPAFGRGTTSFNRFLGDPDNKPNPC
VAPLKKGPFYAIKLVMGDLGTFDGLATSVTGEVLRKDGTRIDGLYAVGNDRASMMGGNYPGAGITHGPNMTFAYLTAKHI
AKEAGAE

Sequences:

>Translated_567_residues
MSQQLTYDLVVIGSGAAGLSCAITARKKGMNVVVLEKEPVFGGTTALSGGVLWVPLTHHGLKQNPQDSIEAVQEFLRSET
GHFYDEVVARAFIEAGPEMVSFFERETEMKFVPTLYPDYHPIAPGGVDVGRSILAAPFDIRSLGKDMQRLKPPLKTITFM
GMMFNSSNADLKHFFRATKSFRSFLYVARRLANHVKEIALYRRAVQVTSGNALAARLAKSALDLNIPILTNAPATRLITD
GDHVKGVVANTEDGEREFLARNGVVLACGGFPHDLNRIGAAYPHLRRGHEHLSPTPKSNTGDGVALAETAGGHADIRYTS
PAAWMPVTKVDFGNGEIGVFPHLLDRYKPGVIGVLSNGARFTNESNSYHDVGSAMIEACAGLKETAMWLICDRETLGKYG
LGYVKPSPMPIGRFLRNGYLYQGRTLDELAHATGIDPAGLAETVHEYNKSAQFGNDPAFGRGTTSFNRFLGDPDNKPNPC
VAPLKKGPFYAIKLVMGDLGTFDGLATSVTGEVLRKDGTRIDGLYAVGNDRASMMGGNYPGAGITHGPNMTFAYLTAKHI
AKEAGAE
>Mature_566_residues
SQQLTYDLVVIGSGAAGLSCAITARKKGMNVVVLEKEPVFGGTTALSGGVLWVPLTHHGLKQNPQDSIEAVQEFLRSETG
HFYDEVVARAFIEAGPEMVSFFERETEMKFVPTLYPDYHPIAPGGVDVGRSILAAPFDIRSLGKDMQRLKPPLKTITFMG
MMFNSSNADLKHFFRATKSFRSFLYVARRLANHVKEIALYRRAVQVTSGNALAARLAKSALDLNIPILTNAPATRLITDG
DHVKGVVANTEDGEREFLARNGVVLACGGFPHDLNRIGAAYPHLRRGHEHLSPTPKSNTGDGVALAETAGGHADIRYTSP
AAWMPVTKVDFGNGEIGVFPHLLDRYKPGVIGVLSNGARFTNESNSYHDVGSAMIEACAGLKETAMWLICDRETLGKYGL
GYVKPSPMPIGRFLRNGYLYQGRTLDELAHATGIDPAGLAETVHEYNKSAQFGNDPAFGRGTTSFNRFLGDPDNKPNPCV
APLKKGPFYAIKLVMGDLGTFDGLATSVTGEVLRKDGTRIDGLYAVGNDRASMMGGNYPGAGITHGPNMTFAYLTAKHIA
KEAGAE

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 61205; Mature: 61074

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQLTYDLVVIGSGAAGLSCAITARKKGMNVVVLEKEPVFGGTTALSGGVLWVPLTHHG
CCCCEEEEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCC
LKQNPQDSIEAVQEFLRSETGHFYDEVVARAFIEAGPEMVSFFERETEMKFVPTLYPDYH
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCEECCCCCCCCC
PIAPGGVDVGRSILAAPFDIRSLGKDMQRLKPPLKTITFMGMMFNSSNADLKHFFRATKS
CCCCCCHHHCHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHEECCCCCHHHHHHHHHHH
FRSFLYVARRLANHVKEIALYRRAVQVTSGNALAARLAKSALDLNIPILTNAPATRLITD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEC
GDHVKGVVANTEDGEREFLARNGVVLACGGFPHDLNRIGAAYPHLRRGHEHLSPTPKSNT
CCCCEEEEECCCCCHHHHHHCCCEEEEECCCCHHHHHHHHCCHHHHCCHHHCCCCCCCCC
GDGVALAETAGGHADIRYTSPAAWMPVTKVDFGNGEIGVFPHLLDRYKPGVIGVLSNGAR
CCCEEEEECCCCCEEEEECCCCCCCCEEEEECCCCCEEHHHHHHHHCCCCEEEEECCCCC
FTNESNSYHDVGSAMIEACAGLKETAMWLICDRETLGKYGLGYVKPSPMPIGRFLRNGYL
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCHHHHHHCCEE
YQGRTLDELAHATGIDPAGLAETVHEYNKSAQFGNDPAFGRGTTSFNRFLGDPDNKPNPC
ECCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCC
VAPLKKGPFYAIKLVMGDLGTFDGLATSVTGEVLRKDGTRIDGLYAVGNDRASMMGGNYP
CCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCCCCEECEEEEECCCHHHHCCCCCC
GAGITHGPNMTFAYLTAKHIAKEAGAE
CCCCCCCCCCEEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQQLTYDLVVIGSGAAGLSCAITARKKGMNVVVLEKEPVFGGTTALSGGVLWVPLTHHG
CCCEEEEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCC
LKQNPQDSIEAVQEFLRSETGHFYDEVVARAFIEAGPEMVSFFERETEMKFVPTLYPDYH
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCEECCCCCCCCC
PIAPGGVDVGRSILAAPFDIRSLGKDMQRLKPPLKTITFMGMMFNSSNADLKHFFRATKS
CCCCCCHHHCHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHEECCCCCHHHHHHHHHHH
FRSFLYVARRLANHVKEIALYRRAVQVTSGNALAARLAKSALDLNIPILTNAPATRLITD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEC
GDHVKGVVANTEDGEREFLARNGVVLACGGFPHDLNRIGAAYPHLRRGHEHLSPTPKSNT
CCCCEEEEECCCCCHHHHHHCCCEEEEECCCCHHHHHHHHCCHHHHCCHHHCCCCCCCCC
GDGVALAETAGGHADIRYTSPAAWMPVTKVDFGNGEIGVFPHLLDRYKPGVIGVLSNGAR
CCCEEEEECCCCCEEEEECCCCCCCCEEEEECCCCCEEHHHHHHHHCCCCEEEEECCCCC
FTNESNSYHDVGSAMIEACAGLKETAMWLICDRETLGKYGLGYVKPSPMPIGRFLRNGYL
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCCCCCCCHHHHHHCCEE
YQGRTLDELAHATGIDPAGLAETVHEYNKSAQFGNDPAFGRGTTSFNRFLGDPDNKPNPC
ECCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCC
VAPLKKGPFYAIKLVMGDLGTFDGLATSVTGEVLRKDGTRIDGLYAVGNDRASMMGGNYP
CCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCCCCEECEEEEECCCHHHHCCCCCC
GAGITHGPNMTFAYLTAKHIAKEAGAE
CCCCCCCCCCEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]