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The map label for this gene is alkK [H]

Identifier: 94314358

GI number: 94314358

Start: 2188367

End: 2190004

Strand: Reverse

Name: alkK [H]

Synonym: Rmet_5439

Alternate gene names: 94314358

Gene position: 2190004-2188367 (Counterclockwise)

Preceding gene: 94314359

Following gene: 94314356

Centisome position: 84.88

GC content: 63.13

Gene sequence:

>1638_bases
ATGCTGGGACTGATGCAGGACCGACCGCTGTTGATTTCATCGCTGATCGAGCACGCGGCTACGTACCATCCAACGCGGGA
GATCGTCGCGAGAACGGTAGAGGGCCCGATCCACCGTACCAACTATGCCGAGATCCATCGGCGTGCCAAACGCGTTGCCA
ACGCGCTGGCGAGTCTCGGTGTCGGGGTGGCGGACCGTGTCGGCACGCTTGCCTGGAACACGCATCGTCATTTCGAACTG
TATTTCGGGGTGTCCGGTTCGGGCGCGATCCTGCACACGGTCAATCCACGTCTGTTCCCCGAGCAGATCGAGTACATCGT
CAACCACGCGGAGGACAAGGTCCTGTTCTTCGATATTGGCTTCGCGCCGCTGGTCGAGAAGCTGGCGCCGGCCTTGAAAT
CCGTCCAGCACTACGTCGCCATGACGGAGCGGGCGAACATGCCGGCGATCGACGTGCCCAACCTGCTTTGCTACGAGGAC
CTGCTTGCGGAGCATAGCGATACGTATGACTGGCCGGAATTGGACGAGCGCGCGGCGTCGTCGCTGTGCTACACGTCGGG
CACCACCGGGAATCCGAAGGGCGTGCTGTACTCGCATCGGTCTACCGTGCTGCATTCGCTCGTGGCGTGCGCGGCGGATG
CGTTCGCCGTGGGCGCGTCAACGTCTGTGCTGCTGGCGGTCCCGATGTTCCATGCCAACGCCTGGGGAATGCCGTATGCG
GCGGCCATGGTCGGGGCCAAGATGGTGATGCCCGGGCCACACCTCAATGGTCAATCGCTCTACGAACTGATGCGCGACGA
AGGCGTAACTGTCTCCCAGGCGGTGCCTACAGTCTGGCTCATGCTCTTCCAGTTCCTGGACGAGCACCCGGAGATCGACA
TGAAGGCGCTTGGCCTGAAGATCATCGGCTCGGGCGGCGCCGCATCGCCGCGCAGCATGATCGAGCGCTGCGAGCGCGAC
TTTGGCGTCGAGTACCTGCAGGCATGGGGCATGACGGAAACCAGCCCGATCGGTACCACCGGCCGCCTGCTGCCCAAGCA
TGCCGCCCTGCCAAAGGACGAGCAGACCACGATCAAGATGAAGGCCGGACGCGCGGTATGGGGCGTAGGTCTGAAGATCA
TCGGCGACGATGGTCAGGAACTGCCGCACGACGGACGCGCCTTCGGTCATCTGCACGTGCGCGGGCCGTGGATCGCAAGC
GGCTACTTCAAGCACACGGGTGGCGAGGTGCTGGACAAGGACGGCTGGTTTCCCACTGGCGATATCGGCACCATCGATCC
GGACGGGTACGTGCAACTGGTGGACCGTGCCAAGGATGTCATCAAGTCGGGTGGGGAATGGATTTCCTCGATCGACCTGG
AAAACGCGGCGATCGGCCACCCTGCCGTGCACGAGGCGGCGATCATTGCCGTCGCGCATCCGAAGTGGCAGGAGCGCCCA
CTGCTGCTGGCGGTGAAGCGTCCCGGCAAGGATGTCACGCGCGAAGAGTTGCTGCAGTACCTGAGCGAGCGCGTCGTGAA
ATGGTGGGTGCCGGACGATGTCGTGTTCGTCGATCAGCTGCCGCATACCGCGACTGGCAAGCTGCTCAAGACGAAGTTGC
GAGAGGACTACCGCAACTATCGATTGCCGACGGCGTGA

Upstream 100 bases:

>100_bases
TGCTGGCGGGAAAGCCAACGACGAAAAGCCAGGCCCGGGCCCCGCCGCATCCTGGCAGCACACCGACAAGCACACCGATA
CATAGAGTGGAGGAGACACG

Downstream 100 bases:

>100_bases
ATCGGGCAGGCGCGGGCATTGCCCGCGCCACGTCTGCCTTGCTACCGCTCAGATCCGCCGGTCGCGCGACTGCCAGTAGA
CTTCACGCAGCTTGTACTTG

Product: Medium-chain-fatty-acid-CoA ligase (Medium-chain acyl-CoA synthetase)

Products: NA

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MLGLMQDRPLLISSLIEHAATYHPTREIVARTVEGPIHRTNYAEIHRRAKRVANALASLGVGVADRVGTLAWNTHRHFEL
YFGVSGSGAILHTVNPRLFPEQIEYIVNHAEDKVLFFDIGFAPLVEKLAPALKSVQHYVAMTERANMPAIDVPNLLCYED
LLAEHSDTYDWPELDERAASSLCYTSGTTGNPKGVLYSHRSTVLHSLVACAADAFAVGASTSVLLAVPMFHANAWGMPYA
AAMVGAKMVMPGPHLNGQSLYELMRDEGVTVSQAVPTVWLMLFQFLDEHPEIDMKALGLKIIGSGGAASPRSMIERCERD
FGVEYLQAWGMTETSPIGTTGRLLPKHAALPKDEQTTIKMKAGRAVWGVGLKIIGDDGQELPHDGRAFGHLHVRGPWIAS
GYFKHTGGEVLDKDGWFPTGDIGTIDPDGYVQLVDRAKDVIKSGGEWISSIDLENAAIGHPAVHEAAIIAVAHPKWQERP
LLLAVKRPGKDVTREELLQYLSERVVKWWVPDDVVFVDQLPHTATGKLLKTKLREDYRNYRLPTA

Sequences:

>Translated_545_residues
MLGLMQDRPLLISSLIEHAATYHPTREIVARTVEGPIHRTNYAEIHRRAKRVANALASLGVGVADRVGTLAWNTHRHFEL
YFGVSGSGAILHTVNPRLFPEQIEYIVNHAEDKVLFFDIGFAPLVEKLAPALKSVQHYVAMTERANMPAIDVPNLLCYED
LLAEHSDTYDWPELDERAASSLCYTSGTTGNPKGVLYSHRSTVLHSLVACAADAFAVGASTSVLLAVPMFHANAWGMPYA
AAMVGAKMVMPGPHLNGQSLYELMRDEGVTVSQAVPTVWLMLFQFLDEHPEIDMKALGLKIIGSGGAASPRSMIERCERD
FGVEYLQAWGMTETSPIGTTGRLLPKHAALPKDEQTTIKMKAGRAVWGVGLKIIGDDGQELPHDGRAFGHLHVRGPWIAS
GYFKHTGGEVLDKDGWFPTGDIGTIDPDGYVQLVDRAKDVIKSGGEWISSIDLENAAIGHPAVHEAAIIAVAHPKWQERP
LLLAVKRPGKDVTREELLQYLSERVVKWWVPDDVVFVDQLPHTATGKLLKTKLREDYRNYRLPTA
>Mature_545_residues
MLGLMQDRPLLISSLIEHAATYHPTREIVARTVEGPIHRTNYAEIHRRAKRVANALASLGVGVADRVGTLAWNTHRHFEL
YFGVSGSGAILHTVNPRLFPEQIEYIVNHAEDKVLFFDIGFAPLVEKLAPALKSVQHYVAMTERANMPAIDVPNLLCYED
LLAEHSDTYDWPELDERAASSLCYTSGTTGNPKGVLYSHRSTVLHSLVACAADAFAVGASTSVLLAVPMFHANAWGMPYA
AAMVGAKMVMPGPHLNGQSLYELMRDEGVTVSQAVPTVWLMLFQFLDEHPEIDMKALGLKIIGSGGAASPRSMIERCERD
FGVEYLQAWGMTETSPIGTTGRLLPKHAALPKDEQTTIKMKAGRAVWGVGLKIIGDDGQELPHDGRAFGHLHVRGPWIAS
GYFKHTGGEVLDKDGWFPTGDIGTIDPDGYVQLVDRAKDVIKSGGEWISSIDLENAAIGHPAVHEAAIIAVAHPKWQERP
LLLAVKRPGKDVTREELLQYLSERVVKWWVPDDVVFVDQLPHTATGKLLKTKLREDYRNYRLPTA

Specific function: Unknown

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=557, Percent_Identity=25.1346499102334, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI187761345, Length=535, Percent_Identity=25.2336448598131, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI187761343, Length=535, Percent_Identity=25.2336448598131, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI38505220, Length=514, Percent_Identity=22.7626459143969, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI157311624, Length=514, Percent_Identity=23.9299610894942, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI157311622, Length=514, Percent_Identity=23.9299610894942, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI13375727, Length=564, Percent_Identity=24.822695035461, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI58082049, Length=518, Percent_Identity=23.7451737451737, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI42544132, Length=513, Percent_Identity=22.8070175438596, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI28416953, Length=538, Percent_Identity=23.0483271375465, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI122937307, Length=514, Percent_Identity=22.7626459143969, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI115511026, Length=522, Percent_Identity=21.455938697318, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI253970489, Length=423, Percent_Identity=25.2955082742317, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI8923896, Length=423, Percent_Identity=25.2955082742317, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI145693145, Length=524, Percent_Identity=25.5725190839695, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI221142682, Length=525, Percent_Identity=24.1904761904762, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788107, Length=523, Percent_Identity=25.2390057361377, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1786810, Length=472, Percent_Identity=26.6949152542373, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789201, Length=375, Percent_Identity=25.8666666666667, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790505, Length=541, Percent_Identity=22.9205175600739, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788595, Length=447, Percent_Identity=23.489932885906, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=510, Percent_Identity=25.8823529411765, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17531443, Length=532, Percent_Identity=22.7443609022556, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32563687, Length=516, Percent_Identity=26.3565891472868, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=530, Percent_Identity=24.7169811320755, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=524, Percent_Identity=23.2824427480916, Blast_Score=129, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=530, Percent_Identity=24.3396226415094, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71994690, Length=569, Percent_Identity=23.3743409490334, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71994703, Length=569, Percent_Identity=23.3743409490334, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71994694, Length=569, Percent_Identity=23.3743409490334, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=537, Percent_Identity=23.463687150838, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=523, Percent_Identity=25.2390057361377, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=360, Percent_Identity=23.6111111111111, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=513, Percent_Identity=22.6120857699805, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556552, Length=200, Percent_Identity=26, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=367, Percent_Identity=27.5204359673025, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21355181, Length=517, Percent_Identity=25.7253384912959, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI18859661, Length=474, Percent_Identity=27.0042194092827, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24581924, Length=552, Percent_Identity=23.9130434782609, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648260, Length=498, Percent_Identity=24.2971887550201, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21356947, Length=496, Percent_Identity=22.7822580645161, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281365686, Length=512, Percent_Identity=21.484375, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24653035, Length=507, Percent_Identity=20.3155818540434, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI161076582, Length=510, Percent_Identity=21.7647058823529, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19922652, Length=500, Percent_Identity=21.6, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20130357, Length=509, Percent_Identity=21.2180746561886, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648253, Length=533, Percent_Identity=22.7016885553471, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24656500, Length=510, Percent_Identity=22.3529411764706, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648255, Length=535, Percent_Identity=22.2429906542056, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21358303, Length=470, Percent_Identity=20.8510638297872, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648257, Length=485, Percent_Identity=22.4742268041237, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62472339, Length=402, Percent_Identity=24.8756218905473, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24667955, Length=402, Percent_Identity=24.8756218905473, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24667959, Length=402, Percent_Identity=24.8756218905473, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 60026; Mature: 60026

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLMQDRPLLISSLIEHAATYHPTREIVARTVEGPIHRTNYAEIHRRAKRVANALASLG
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHC
VGVADRVGTLAWNTHRHFELYFGVSGSGAILHTVNPRLFPEQIEYIVNHAEDKVLFFDIG
CCHHHHHHHEEECCCCEEEEEEEECCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEECC
FAPLVEKLAPALKSVQHYVAMTERANMPAIDVPNLLCYEDLLAEHSDTYDWPELDERAAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
SLCYTSGTTGNPKGVLYSHRSTVLHSLVACAADAFAVGASTSVLLAVPMFHANAWGMPYA
HEEECCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCHHH
AAMVGAKMVMPGPHLNGQSLYELMRDEGVTVSQAVPTVWLMLFQFLDEHPEIDMKALGLK
HHHHCCCEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECEE
IIGSGGAASPRSMIERCERDFGVEYLQAWGMTETSPIGTTGRLLPKHAALPKDEQTTIKM
EEECCCCCCHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEE
KAGRAVWGVGLKIIGDDGQELPHDGRAFGHLHVRGPWIASGYFKHTGGEVLDKDGWFPTG
ECCCEEEECCEEEECCCCCCCCCCCCEEEEEEEECCEEECCCCCCCCCCEECCCCCCCCC
DIGTIDPDGYVQLVDRAKDVIKSGGEWISSIDLENAAIGHPAVHEAAIIAVAHPKWQERP
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
LLLAVKRPGKDVTREELLQYLSERVVKWWVPDDVVFVDQLPHTATGKLLKTKLREDYRNY
EEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
RLPTA
CCCCC
>Mature Secondary Structure
MLGLMQDRPLLISSLIEHAATYHPTREIVARTVEGPIHRTNYAEIHRRAKRVANALASLG
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHC
VGVADRVGTLAWNTHRHFELYFGVSGSGAILHTVNPRLFPEQIEYIVNHAEDKVLFFDIG
CCHHHHHHHEEECCCCEEEEEEEECCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEECC
FAPLVEKLAPALKSVQHYVAMTERANMPAIDVPNLLCYEDLLAEHSDTYDWPELDERAAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
SLCYTSGTTGNPKGVLYSHRSTVLHSLVACAADAFAVGASTSVLLAVPMFHANAWGMPYA
HEEECCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCHHH
AAMVGAKMVMPGPHLNGQSLYELMRDEGVTVSQAVPTVWLMLFQFLDEHPEIDMKALGLK
HHHHCCCEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECEE
IIGSGGAASPRSMIERCERDFGVEYLQAWGMTETSPIGTTGRLLPKHAALPKDEQTTIKM
EEECCCCCCHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEE
KAGRAVWGVGLKIIGDDGQELPHDGRAFGHLHVRGPWIASGYFKHTGGEVLDKDGWFPTG
ECCCEEEECCEEEECCCCCCCCCCCCEEEEEEEECCEEECCCCCCCCCCEECCCCCCCCC
DIGTIDPDGYVQLVDRAKDVIKSGGEWISSIDLENAAIGHPAVHEAAIIAVAHPKWQERP
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
LLLAVKRPGKDVTREELLQYLSERVVKWWVPDDVVFVDQLPHTATGKLLKTKLREDYRNY
EEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
RLPTA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]