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The map label for this gene is caiD [C]

Identifier: 94314350

GI number: 94314350

Start: 2177192

End: 2178034

Strand: Reverse

Name: caiD [C]

Synonym: Rmet_5431

Alternate gene names: 94314350

Gene position: 2178034-2177192 (Counterclockwise)

Preceding gene: 94314351

Following gene: 94314348

Centisome position: 84.42

GC content: 64.53

Gene sequence:

>843_bases
ATGCTGTATCAAAGAGAAGCCCTTGGAATGACTGCTGACAACTCCCAACGTATCGTTGTAACCATCGAGGAAGGCGTGGC
CGAAGTCCGCCTGAATCGCCCCGACAAGATGAACGCGCTGGATCAGGCGATGTTCGACGCATTGGTCGACACGGGCCGGC
AACTGGCCGAGATGCCAGGTCTGCGTGCTGTCGTGCTTTCCGGTGAAGGCAAGGCGTTCTGCGCGGGTCTGGACATGGGC
CGCATGGCCAGTCTGAGCGAAGGGACCGACCCCTCGCTCAAGCGTGGCCGGCTGACGCCGCGCACGCATGGCATGTCGAA
TCACCCGCAATACGCCTGCATGGTCTGGCGCGAACTGCCCGTGCCAGTGATCGCCGCGGTGCATGGCGCTGCCTTTGGCG
GTGGGCTCCAGGTGGCGCTGGGCGCGGACCTGCGCTACGTGACGGCCGATGCCAAGCTTTCGATCATGGAGATCAAGTGG
GGTCTGGTGCCGGATATGGGCGGCATGTTGCTGACGCGCGGGCTCGTACGGCCAGATGTGCTGAACGAACTGATCTACAC
GGGACGCATCATCAGCGGCACCGAGGCGGTGGAACTGGGGCTGGCCACGCGCGTGTGTGAAGATCCAAGGGTCGCGGCAA
TGGCCGTCGCACGCGAGATCGCCATGAAGAGCCCGGACGCGATTCGTGCGGGCAAACGCCTGATGCGGGTCACCGAGGAT
GGCGACGCGCTGGCGATTCTCCAGGCCGAGTCTGTCGAGCAGGACGCGCTGATCGGCGCGCCCAACCAGACGGAGGCGGT
ACGCGCCAATATGGAGAAGCGGGCGCCGCGATTCCTGCCGTGA

Upstream 100 bases:

>100_bases
ACGCGTGGAGCGTGTCCGACGGGATTTTTTTGGTCTGCGGTGCCATGTTCGGCGCGCCCTGGAAGCCAGTTACCGAAATC
CGGGATGTGACCGCTGCAAC

Downstream 100 bases:

>100_bases
TGGCTTGAGTCTTGTCTCGGGGGCGCGCTACCGCTGTGGCTGGCCGTGCGCGGACGCCCTTCGGGTACATCAAGGGGCGA
GAGGTGCTGGCGGACCCTGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MLYQREALGMTADNSQRIVVTIEEGVAEVRLNRPDKMNALDQAMFDALVDTGRQLAEMPGLRAVVLSGEGKAFCAGLDMG
RMASLSEGTDPSLKRGRLTPRTHGMSNHPQYACMVWRELPVPVIAAVHGAAFGGGLQVALGADLRYVTADAKLSIMEIKW
GLVPDMGGMLLTRGLVRPDVLNELIYTGRIISGTEAVELGLATRVCEDPRVAAMAVAREIAMKSPDAIRAGKRLMRVTED
GDALAILQAESVEQDALIGAPNQTEAVRANMEKRAPRFLP

Sequences:

>Translated_280_residues
MLYQREALGMTADNSQRIVVTIEEGVAEVRLNRPDKMNALDQAMFDALVDTGRQLAEMPGLRAVVLSGEGKAFCAGLDMG
RMASLSEGTDPSLKRGRLTPRTHGMSNHPQYACMVWRELPVPVIAAVHGAAFGGGLQVALGADLRYVTADAKLSIMEIKW
GLVPDMGGMLLTRGLVRPDVLNELIYTGRIISGTEAVELGLATRVCEDPRVAAMAVAREIAMKSPDAIRAGKRLMRVTED
GDALAILQAESVEQDALIGAPNQTEAVRANMEKRAPRFLP
>Mature_280_residues
MLYQREALGMTADNSQRIVVTIEEGVAEVRLNRPDKMNALDQAMFDALVDTGRQLAEMPGLRAVVLSGEGKAFCAGLDMG
RMASLSEGTDPSLKRGRLTPRTHGMSNHPQYACMVWRELPVPVIAAVHGAAFGGGLQVALGADLRYVTADAKLSIMEIKW
GLVPDMGGMLLTRGLVRPDVLNELIYTGRIISGTEAVELGLATRVCEDPRVAAMAVAREIAMKSPDAIRAGKRLMRVTED
GDALAILQAESVEQDALIGAPNQTEAVRANMEKRAPRFLP

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=277, Percent_Identity=31.7689530685921, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=30.1158301158301, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=26.7441860465116, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI37594471, Length=259, Percent_Identity=26.6409266409266, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI37594469, Length=259, Percent_Identity=26.6409266409266, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4502327, Length=278, Percent_Identity=27.6978417266187, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI68989263, Length=225, Percent_Identity=28, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI221142681, Length=231, Percent_Identity=32.4675324675325, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787659, Length=266, Percent_Identity=26.6917293233083, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1790281, Length=203, Percent_Identity=29.5566502463054, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=31.203007518797, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082183, Length=271, Percent_Identity=27.3062730627306, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788682, Length=204, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=228, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=236, Percent_Identity=30.9322033898305, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=241, Percent_Identity=29.4605809128631, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=29.5719844357977, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=27.1317829457364, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=210, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=215, Percent_Identity=28.8372093023256, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19920382, Length=214, Percent_Identity=34.5794392523364, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=28.7878787878788, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30163; Mature: 30163

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYQREALGMTADNSQRIVVTIEEGVAEVRLNRPDKMNALDQAMFDALVDTGRQLAEMPG
CCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHCCC
LRAVVLSGEGKAFCAGLDMGRMASLSEGTDPSLKRGRLTPRTHGMSNHPQYACMVWRELP
CEEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEEHHCC
VPVIAAVHGAAFGGGLQVALGADLRYVTADAKLSIMEIKWGLVPDMGGMLLTRGLVRPDV
CHHHHHHHHHCCCCCEEEEECCCEEEEECCCEEEEEEEECCCCCCCCHHHHHHCCCCHHH
LNELIYTGRIISGTEAVELGLATRVCEDPRVAAMAVAREIAMKSPDAIRAGKRLMRVTED
HHHHHHHCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHEEEECC
GDALAILQAESVEQDALIGAPNQTEAVRANMEKRAPRFLP
CCEEEEEECCCCCCCCEECCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLYQREALGMTADNSQRIVVTIEEGVAEVRLNRPDKMNALDQAMFDALVDTGRQLAEMPG
CCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHCCC
LRAVVLSGEGKAFCAGLDMGRMASLSEGTDPSLKRGRLTPRTHGMSNHPQYACMVWRELP
CEEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEEHHCC
VPVIAAVHGAAFGGGLQVALGADLRYVTADAKLSIMEIKWGLVPDMGGMLLTRGLVRPDV
CHHHHHHHHHCCCCCEEEEECCCEEEEECCCEEEEEEEECCCCCCCCHHHHHHCCCCHHH
LNELIYTGRIISGTEAVELGLATRVCEDPRVAAMAVAREIAMKSPDAIRAGKRLMRVTED
HHHHHHHCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHEEEECC
GDALAILQAESVEQDALIGAPNQTEAVRANMEKRAPRFLP
CCEEEEEECCCCCCCCEECCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA