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The map label for this gene is htrB [H]

Identifier: 94314349

GI number: 94314349

Start: 2176193

End: 2177122

Strand: Direct

Name: htrB [H]

Synonym: Rmet_5430

Alternate gene names: 94314349

Gene position: 2176193-2177122 (Clockwise)

Preceding gene: 94314346

Following gene: 94314357

Centisome position: 84.35

GC content: 65.05

Gene sequence:

>930_bases
ATGCACCGAAATCAACCTCCGCCGCGGAGGGTGCTGTCCACTCTCATCCGGCTGATTGCCGCCGTGCCGCTACCGATTGC
GCACGCTACAGGCCGCGTTCTGGGGCGGCTCGTCTATGCCCTCCCGGGGCGATACCGACAGCACGTGCAGCAGAATGCCG
CCCAGGCTGGCTTTTCCGATCCGGCATTTGCCCGGCAGGCCGCAGGCGAGACCGGCGCCATGATGATCGAACTGGCACGC
GTGTGGTATCGCGGCCCGGCGTGTCGCGATCAGGTGATTTGCGATGACTGGACGGTGATCGAGCAGACCCTGGCGGAAGG
TCGCGGACTCATCATGCTGACACCGCACCTTGGATGCTTCGAACTGGCAGCCCGCTATGCCGCGGAGCGCATCGCGCTGA
CAGTCATGTTCCGACCACACCGCAAGCCGATATTGCGGGACTTGCTTGAATCGGTACGTGCCACCTCGAGTCTGAAGACA
GTTCCCGCGACTTCCCAAGGCGTGCGCGCGTTCGTCCGCGCGCTGCGCCAGGGTGAAGCGATCGGGCTATTGCCCGATCA
GGCGCCCAAGGGTGAAGGCGTGTGGGTACCGTTCTTTGGCCGCAAGGCCTATACGATAACGCTGCCAGGAAAGCTCGCAG
CGCAAACGGGGGCGCCGCTGATCGTTGCCGCGGGCGAACGCCTGCCCGGTGGGCGTGGCTGGCGCATGCACGTGAAGCGC
CTGTCAGGACCATTGCCGCCGACCGCCGAGGCGCAGGCAGCATGGATCAACGCCGAACTGGAAGAGATGATCCGGCGCTT
TCCGCTGCAGTACCTGTGGGGATACAACCGCTTCAAGGTCCCGTCCGGCGCTCCGGCTCCCGATTCAGCCGTTGTCCCAG
AACACGACGCCTCCGTTATCCAGGGTCCGCCAGCACCTCTCGCCCCTTGA

Upstream 100 bases:

>100_bases
GAAGAGCGGCCCCTAACAATCCGGACAAGTATCTGCCCGATTCTTCGTGTAGAAGAAATTTGTCCCGCGACACCCAATAC
TCAACCCCCGAGGAGTGACC

Downstream 100 bases:

>100_bases
TGTACCCGAAGGGCGTCCGCGCACGGCCAGCCACAGCGGTAGCGCGCCCCCGAGACAAGACTCAAGCCATCACGGCAGGA
ATCGCGGCGCCCGCTTCTCC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MHRNQPPPRRVLSTLIRLIAAVPLPIAHATGRVLGRLVYALPGRYRQHVQQNAAQAGFSDPAFARQAAGETGAMMIELAR
VWYRGPACRDQVICDDWTVIEQTLAEGRGLIMLTPHLGCFELAARYAAERIALTVMFRPHRKPILRDLLESVRATSSLKT
VPATSQGVRAFVRALRQGEAIGLLPDQAPKGEGVWVPFFGRKAYTITLPGKLAAQTGAPLIVAAGERLPGGRGWRMHVKR
LSGPLPPTAEAQAAWINAELEEMIRRFPLQYLWGYNRFKVPSGAPAPDSAVVPEHDASVIQGPPAPLAP

Sequences:

>Translated_309_residues
MHRNQPPPRRVLSTLIRLIAAVPLPIAHATGRVLGRLVYALPGRYRQHVQQNAAQAGFSDPAFARQAAGETGAMMIELAR
VWYRGPACRDQVICDDWTVIEQTLAEGRGLIMLTPHLGCFELAARYAAERIALTVMFRPHRKPILRDLLESVRATSSLKT
VPATSQGVRAFVRALRQGEAIGLLPDQAPKGEGVWVPFFGRKAYTITLPGKLAAQTGAPLIVAAGERLPGGRGWRMHVKR
LSGPLPPTAEAQAAWINAELEEMIRRFPLQYLWGYNRFKVPSGAPAPDSAVVPEHDASVIQGPPAPLAP
>Mature_309_residues
MHRNQPPPRRVLSTLIRLIAAVPLPIAHATGRVLGRLVYALPGRYRQHVQQNAAQAGFSDPAFARQAAGETGAMMIELAR
VWYRGPACRDQVICDDWTVIEQTLAEGRGLIMLTPHLGCFELAARYAAERIALTVMFRPHRKPILRDLLESVRATSSLKT
VPATSQGVRAFVRALRQGEAIGLLPDQAPKGEGVWVPFFGRKAYTITLPGKLAAQTGAPLIVAAGERLPGGRGWRMHVKR
LSGPLPPTAEAQAAWINAELEEMIRRFPLQYLWGYNRFKVPSGAPAPDSAVVPEHDASVIQGPPAPLAP

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=300, Percent_Identity=27.6666666666667, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082094, Length=214, Percent_Identity=24.7663551401869, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 33707; Mature: 33707

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRNQPPPRRVLSTLIRLIAAVPLPIAHATGRVLGRLVYALPGRYRQHVQQNAAQAGFSD
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
PAFARQAAGETGAMMIELARVWYRGPACRDQVICDDWTVIEQTLAEGRGLIMLTPHLGCF
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHCCCCEEEECCCCCHH
ELAARYAAERIALTVMFRPHRKPILRDLLESVRATSSLKTVPATSQGVRAFVRALRQGEA
HHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCE
IGLLPDQAPKGEGVWVPFFGRKAYTITLPGKLAAQTGAPLIVAAGERLPGGRGWRMHVKR
EECCCCCCCCCCEEEEEEECCEEEEEECCCHHHHCCCCCEEEECCCCCCCCCCHHHHHHH
LSGPLPPTAEAQAAWINAELEEMIRRFPLQYLWGYNRFKVPSGAPAPDSAVVPEHDASVI
HCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHH
QGPPAPLAP
CCCCCCCCC
>Mature Secondary Structure
MHRNQPPPRRVLSTLIRLIAAVPLPIAHATGRVLGRLVYALPGRYRQHVQQNAAQAGFSD
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
PAFARQAAGETGAMMIELARVWYRGPACRDQVICDDWTVIEQTLAEGRGLIMLTPHLGCF
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHCCCCEEEECCCCCHH
ELAARYAAERIALTVMFRPHRKPILRDLLESVRATSSLKTVPATSQGVRAFVRALRQGEA
HHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCE
IGLLPDQAPKGEGVWVPFFGRKAYTITLPGKLAAQTGAPLIVAAGERLPGGRGWRMHVKR
EECCCCCCCCCCEEEEEEECCEEEEEECCCHHHHCCCCCEEEECCCCCCCCCCHHHHHHH
LSGPLPPTAEAQAAWINAELEEMIRRFPLQYLWGYNRFKVPSGAPAPDSAVVPEHDASVI
HCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHH
QGPPAPLAP
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]