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The map label for this gene is betA4 [H]

Identifier: 94314333

GI number: 94314333

Start: 2156822

End: 2158426

Strand: Direct

Name: betA4 [H]

Synonym: Rmet_5414

Alternate gene names: 94314333

Gene position: 2156822-2158426 (Clockwise)

Preceding gene: 94314332

Following gene: 94314334

Centisome position: 83.6

GC content: 67.41

Gene sequence:

>1605_bases
ATGGATTTCGACTACGTCATCATTGGCGCGGGTTCGGCTGGCTGCGTGCTGGCCAACCGCCTGAGCGCCGACCCCTCCGT
GCGCGTCTGTCTGGTCGAAGCCGGCCCGGTCGATCGCTCTCCGCTGATCCACACGCCGGCTGGCATCGTCGGCATCCTGC
CGACCCGACACGTCAACTGGGCATTCGAGACAGTTCCGCAGCCAGGGCTGAAGGGCCGTCTCGGCTACCAGCCGCGCGGC
AAGACGCTTGGCGGCAGCAGCGCCATCAACGCGATGATCTACATCCGCGGCCACCGCACCGATTACGACGACTGGGCTGC
GCTGGGTAATCCCGACTGGTCCTACGACGGTGTGCTGCCCTGGTTCCGACGATCCGAGGATTACTTCGGCGGCGCCGACG
CTTATCACGGCGCCGGCGGCGAGCTGACCGTGAGCGCGCTCGACGCCCATCCGGCCACGCATGCGTTTATCGAAGCCGGC
CGCAAGAGCGGCCACGCCGTCAATGCGGATTTCAACGGCGCCGACCAGGAAGGCGTTGGCCACTATCATGTGACCATTCG
CAACGGCCAGCGCTGCAGCGCATCGGTGGCGTTCCTGCATCCCCTCCGCGACAAGCGCCCGAATCTCACCGTGCTGACGG
GCGGCCATGCCACCCGGCTGATCCTGCGCGGCAACACGGCGGAAGGCGTCACGGTCCGCGTGAAAGGCCGTGACGTGGAA
CTGCACGCGCGCCGCGAGACCATCGTGGCAGCCGGCGCGTTCGGCACACCCCAACTGCTCCAGCTTTCGGGCATCGGCGA
CGAAGCAGACCTGCGTCCCCATGGCATCGCCGTCCAGCACGCGCTGCCCGGCGTCGGCAAGGGGCTGATCGACCATCCCG
ACTACATCCTGCCCTTCAAGAGCCCCGACAAATCGCTGATGGGCGTGTCGCTGCAAGGCGCGGCGGCCATGACGAAGGCG
TTCTTCGAGTACCGCAAGTCGCGCACAGGTCTGCTGGCCAGCAACTTCGGCGAGGCCGGCGCGTTCCTGCGCACCGATCC
GTCGCTGTCGCGTCCCGACGTGCAACTGCACTGGGTCACCGGCATCGTGGACAACCACAACCGCACGCTCCACACGGGAC
ACGGCATGAGCTGCCACGTCTGCGTGCTGCGTCCGAAGAGCCGCGGCACGGTCGGCCTGAACTCCGCCAATCCGCTGGCG
CCGCCGCGTATCGACCCGAACTTCCTCAGCAACGACGACGACGTGACGACGCTGCTCAAGGGCTACAAGCTCTCGCGCGA
GATCATGCACGCCCAGCCGCTGGCCCGCTACGCCGGGCGCGAACTGTACGTGAAGGGTGTGAGCAGCGACGACCAGCTCG
TCGACCTGCTGCGCCGCCGCACCGACACCATCTACCACCCGGTGGGCACCTGCCGCATGGGTAGCGACGACATGGCCGTG
GTCGACCAGCGCCTGCGCGTGCGCGGCATGGAACGACTGCGCGTGGTCGATGCCTCGATCATGCCGACGCTGATCGGCGG
CAACACGAATGCCCCCTCGATCATGATCGGAGAGAAGGGTGCCGCGATGATCGCGGAAGACTGGCAGGGCCGCGCTGCCG
CCTGA

Upstream 100 bases:

>100_bases
ATGTTCCTGGGGCGGCCTACCGCAGCGCCCCCCGTGCAAACCGAAGCCTCGCCAGTGACGGCATCCCAAGACTAATTCCC
AAGTTCCCAAGAACAACATC

Downstream 100 bases:

>100_bases
TTGCGCATCGCAACGCCGCATCACAAAAGCCAAATAGCAAACCCGCATAACAAGAGCCCGAAAAGGAGACTCCATGCAAT
GCAAGACCGCCGCGTTTCAC

Product: choline dehydrogenase, a flavoprotein

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MDFDYVIIGAGSAGCVLANRLSADPSVRVCLVEAGPVDRSPLIHTPAGIVGILPTRHVNWAFETVPQPGLKGRLGYQPRG
KTLGGSSAINAMIYIRGHRTDYDDWAALGNPDWSYDGVLPWFRRSEDYFGGADAYHGAGGELTVSALDAHPATHAFIEAG
RKSGHAVNADFNGADQEGVGHYHVTIRNGQRCSASVAFLHPLRDKRPNLTVLTGGHATRLILRGNTAEGVTVRVKGRDVE
LHARRETIVAAGAFGTPQLLQLSGIGDEADLRPHGIAVQHALPGVGKGLIDHPDYILPFKSPDKSLMGVSLQGAAAMTKA
FFEYRKSRTGLLASNFGEAGAFLRTDPSLSRPDVQLHWVTGIVDNHNRTLHTGHGMSCHVCVLRPKSRGTVGLNSANPLA
PPRIDPNFLSNDDDVTTLLKGYKLSREIMHAQPLARYAGRELYVKGVSSDDQLVDLLRRRTDTIYHPVGTCRMGSDDMAV
VDQRLRVRGMERLRVVDASIMPTLIGGNTNAPSIMIGEKGAAMIAEDWQGRAAA

Sequences:

>Translated_534_residues
MDFDYVIIGAGSAGCVLANRLSADPSVRVCLVEAGPVDRSPLIHTPAGIVGILPTRHVNWAFETVPQPGLKGRLGYQPRG
KTLGGSSAINAMIYIRGHRTDYDDWAALGNPDWSYDGVLPWFRRSEDYFGGADAYHGAGGELTVSALDAHPATHAFIEAG
RKSGHAVNADFNGADQEGVGHYHVTIRNGQRCSASVAFLHPLRDKRPNLTVLTGGHATRLILRGNTAEGVTVRVKGRDVE
LHARRETIVAAGAFGTPQLLQLSGIGDEADLRPHGIAVQHALPGVGKGLIDHPDYILPFKSPDKSLMGVSLQGAAAMTKA
FFEYRKSRTGLLASNFGEAGAFLRTDPSLSRPDVQLHWVTGIVDNHNRTLHTGHGMSCHVCVLRPKSRGTVGLNSANPLA
PPRIDPNFLSNDDDVTTLLKGYKLSREIMHAQPLARYAGRELYVKGVSSDDQLVDLLRRRTDTIYHPVGTCRMGSDDMAV
VDQRLRVRGMERLRVVDASIMPTLIGGNTNAPSIMIGEKGAAMIAEDWQGRAAA
>Mature_534_residues
MDFDYVIIGAGSAGCVLANRLSADPSVRVCLVEAGPVDRSPLIHTPAGIVGILPTRHVNWAFETVPQPGLKGRLGYQPRG
KTLGGSSAINAMIYIRGHRTDYDDWAALGNPDWSYDGVLPWFRRSEDYFGGADAYHGAGGELTVSALDAHPATHAFIEAG
RKSGHAVNADFNGADQEGVGHYHVTIRNGQRCSASVAFLHPLRDKRPNLTVLTGGHATRLILRGNTAEGVTVRVKGRDVE
LHARRETIVAAGAFGTPQLLQLSGIGDEADLRPHGIAVQHALPGVGKGLIDHPDYILPFKSPDKSLMGVSLQGAAAMTKA
FFEYRKSRTGLLASNFGEAGAFLRTDPSLSRPDVQLHWVTGIVDNHNRTLHTGHGMSCHVCVLRPKSRGTVGLNSANPLA
PPRIDPNFLSNDDDVTTLLKGYKLSREIMHAQPLARYAGRELYVKGVSSDDQLVDLLRRRTDTIYHPVGTCRMGSDDMAV
VDQRLRVRGMERLRVVDASIMPTLIGGNTNAPSIMIGEKGAAMIAEDWQGRAAA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=534, Percent_Identity=41.3857677902622, Blast_Score=380, Evalue=1e-105,
Organism=Escherichia coli, GI1786503, Length=534, Percent_Identity=40.4494382022472, Blast_Score=349, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17532301, Length=543, Percent_Identity=39.4106813996317, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24642042, Length=572, Percent_Identity=40.2097902097902, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI45551458, Length=583, Percent_Identity=38.7650085763293, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI45549471, Length=583, Percent_Identity=38.7650085763293, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24642035, Length=572, Percent_Identity=38.2867132867133, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI17137792, Length=561, Percent_Identity=37.4331550802139, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24642048, Length=585, Percent_Identity=37.2649572649573, Blast_Score=316, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24642059, Length=577, Percent_Identity=36.3951473136915, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI24642037, Length=573, Percent_Identity=37.347294938918, Blast_Score=312, Evalue=5e-85,
Organism=Drosophila melanogaster, GI24650267, Length=572, Percent_Identity=35.6643356643357, Blast_Score=302, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24642055, Length=572, Percent_Identity=35.8391608391608, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24642039, Length=568, Percent_Identity=34.6830985915493, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24642051, Length=574, Percent_Identity=33.4494773519164, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI18859995, Length=566, Percent_Identity=34.6289752650177, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI18859993, Length=572, Percent_Identity=33.7412587412587, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24645930, Length=586, Percent_Identity=29.3515358361775, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24642057, Length=600, Percent_Identity=29.6666666666667, Blast_Score=161, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 57561; Mature: 57561

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFDYVIIGAGSAGCVLANRLSADPSVRVCLVEAGPVDRSPLIHTPAGIVGILPTRHVNW
CCCCEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCCCCCEEECCCCEEEECCCCCCCE
AFETVPQPGLKGRLGYQPRGKTLGGSSAINAMIYIRGHRTDYDDWAALGNPDWSYDGVLP
EEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHCCCCCCCCCCCCH
WFRRSEDYFGGADAYHGAGGELTVSALDAHPATHAFIEAGRKSGHAVNADFNGADQEGVG
HHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCEEECCCCCCCCCCCC
HYHVTIRNGQRCSASVAFLHPLRDKRPNLTVLTGGHATRLILRGNTAEGVTVRVKGRDVE
EEEEEEECCCCCCEEEEEEEHHHCCCCCEEEEECCCEEEEEEECCCCCCEEEEEECCCEE
LHARRETIVAAGAFGTPQLLQLSGIGDEADLRPHGIAVQHALPGVGKGLIDHPDYILPFK
EEECCEEEEEECCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECC
SPDKSLMGVSLQGAAAMTKAFFEYRKSRTGLLASNFGEAGAFLRTDPSLSRPDVQLHWVT
CCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEECCCCCCCCEEECCCCCCCCCEEEEEEE
GIVDNHNRTLHTGHGMSCHVCVLRPKSRGTVGLNSANPLAPPRIDPNFLSNDDDVTTLLK
EEEECCCCEEECCCCCEEEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
GYKLSREIMHAQPLARYAGRELYVKGVSSDDQLVDLLRRRTDTIYHPVGTCRMGSDDMAV
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCEECCCCEEEECCCCHHH
VDQRLRVRGMERLRVVDASIMPTLIGGNTNAPSIMIGEKGAAMIAEDWQGRAAA
HHHHHHHHCHHHHEEECHHHCCEEECCCCCCCEEEECCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MDFDYVIIGAGSAGCVLANRLSADPSVRVCLVEAGPVDRSPLIHTPAGIVGILPTRHVNW
CCCCEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCCCCCEEECCCCEEEECCCCCCCE
AFETVPQPGLKGRLGYQPRGKTLGGSSAINAMIYIRGHRTDYDDWAALGNPDWSYDGVLP
EEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHCCCCCCCCCCCCH
WFRRSEDYFGGADAYHGAGGELTVSALDAHPATHAFIEAGRKSGHAVNADFNGADQEGVG
HHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCEEECCCCCCCCCCCC
HYHVTIRNGQRCSASVAFLHPLRDKRPNLTVLTGGHATRLILRGNTAEGVTVRVKGRDVE
EEEEEEECCCCCCEEEEEEEHHHCCCCCEEEEECCCEEEEEEECCCCCCEEEEEECCCEE
LHARRETIVAAGAFGTPQLLQLSGIGDEADLRPHGIAVQHALPGVGKGLIDHPDYILPFK
EEECCEEEEEECCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECC
SPDKSLMGVSLQGAAAMTKAFFEYRKSRTGLLASNFGEAGAFLRTDPSLSRPDVQLHWVT
CCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEECCCCCCCCEEECCCCCCCCCEEEEEEE
GIVDNHNRTLHTGHGMSCHVCVLRPKSRGTVGLNSANPLAPPRIDPNFLSNDDDVTTLLK
EEEECCCCEEECCCCCEEEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
GYKLSREIMHAQPLARYAGRELYVKGVSSDDQLVDLLRRRTDTIYHPVGTCRMGSDDMAV
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCEECCCCEEEECCCCHHH
VDQRLRVRGMERLRVVDASIMPTLIGGNTNAPSIMIGEKGAAMIAEDWQGRAAA
HHHHHHHHCHHHHEEECHHHCCEEECCCCCCCEEEECCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]