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The map label for this gene is irlS [H]

Identifier: 94314251

GI number: 94314251

Start: 2044096

End: 2045511

Strand: Direct

Name: irlS [H]

Synonym: Rmet_5332

Alternate gene names: 94314251

Gene position: 2044096-2045511 (Clockwise)

Preceding gene: 94314250

Following gene: 94314253

Centisome position: 79.23

GC content: 69.28

Gene sequence:

>1416_bases
GTGCTGAACTTCCGGCAGCGGCTGGTCCTGACCCACTGGACCGTCATCGTGGTGATCGTGGCCTGCTCGGCGTTGGCCGG
GTGGTGGCAGCTATCGCGCCTGGTGCACGGGCAGCTCGACGCGGCGCTGCTGACGCTGGCCGATACCGAGGCCGGCATGC
AGGTGGAGAGCCACGGGCAACCGGTCCGCGTGCACGAGCGCCCGCCCGGGTCAGCCCCGCCATCGCTAGTGCGCCTGGAC
CGGCTGGTGCAGATCGTCAATGCCAAGGGCGAAATCCTGGCCCGCAGCTCAAACCTGGGCGACGCCATGCTGCCCGCTCC
GCCCGCGCTGCTCGACGAACTCGCGTCCGGGGGGCAGGCGGTCTTTCAGACGCTGCCGAGCTTTGGGGAAGAGCCGCTGC
GCATGGTGTCCCTACCCGCCGTGGCAAACGGCAAGCCCGTGGTGATCCAGGTGGCGGGATCGCTGGACGATGTGCACAAC
GTGCTGGAGTCGGCCACGATCCTGTTCGGCATCATGGCCGCCGCACTGCTGGCCGCCGTGGGCGCCGCCGGCAGCGTGCT
GACGCGGCGCGCGTTCCGTGGCATCAACGACGTGGTGAAGCAAGCCCGGCTCATCGGCGATGCCAACCTTGGCGAACGCC
TGCCGCACCCCGGCACAACGGACGAAATCGGCCAGCTCGTCGATACGCTGAACGCGATGCTGGACCGGCTCGAGCAGGCT
TTCGACCAGCAGCGGCGCTTCACGGCCGACGCCTCGCACGAACTGCGCTCGCCGCTTTCCCGCCTGCGCACCGAGATCGA
AGTGACGTTGCGGCGGCCGCGCGAGAGCCAGGAGTACGTGGACACGCTGCGCTCCTGCCTGGATGAAGTGGGCCGCCTGA
CCCGGCTGATCGAGGAACTGCTGATGCTGGCCCGCTTCGACGCGGGGCAGGATCGCCAGGCTGGCGAGCCACAGGCACTG
GATGCGCTGATCGCGGAAACGCTGTTGCGCGCCGGCCCAGCCGCCCGTGAACGGCAGGTCGAACTGGTGGCACGCAGCGA
AGCGGTGGGCGGCGCCAAGGTGTCGCACGGATCGGTGGGGCTGGTGCTGGCCAATCTGGTGGACAATGCGCTCAAGTTCT
CGCCACAGGGCAGCAAGATCGTGGTGGACTGCGCCGCCGACGCGCACCAGGCCACATTGCGTGTCACCGACGAAGGCCCC
GGCATCCCCCCGGACGAGTTGCCCTTCCTGTTCGACCGCTTCTTCCGTGGCGCTGGCGCGCGCGCGTCCGAGAACGAAGG
AGTGGGTCTTGGCCTGGCCCTGTCGCAATCGATCATCCATGCCCAGGGCGGGCGTATCGAGGCCGCCAACGGGCCCGACG
GCGGCGCGGTGTTCACGGTCAGGATTCCGCTGGCGCCGCGTGAAGCCGGTTCCTGA

Upstream 100 bases:

>100_bases
AAGAACCTGCGCCGCAAGCTCGACCCACCGGGCATGGCGGCGCTGATCCAAACCGTGCGGGGCCAGGGCTTCCGCATCTC
GCCATCGGGGGCCCTCTGAC

Downstream 100 bases:

>100_bases
AGAAGAATTCAGTGTACGGAGCAGCACCCCGTCGATACACTGCGTCCCATCAAAGCTCGCCTGGGGAGGACCCTCAAATG
GACTGTTGTTCTAGTCGATA

Product: histidine kinase sensor two components regulatory response

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MLNFRQRLVLTHWTVIVVIVACSALAGWWQLSRLVHGQLDAALLTLADTEAGMQVESHGQPVRVHERPPGSAPPSLVRLD
RLVQIVNAKGEILARSSNLGDAMLPAPPALLDELASGGQAVFQTLPSFGEEPLRMVSLPAVANGKPVVIQVAGSLDDVHN
VLESATILFGIMAAALLAAVGAAGSVLTRRAFRGINDVVKQARLIGDANLGERLPHPGTTDEIGQLVDTLNAMLDRLEQA
FDQQRRFTADASHELRSPLSRLRTEIEVTLRRPRESQEYVDTLRSCLDEVGRLTRLIEELLMLARFDAGQDRQAGEPQAL
DALIAETLLRAGPAARERQVELVARSEAVGGAKVSHGSVGLVLANLVDNALKFSPQGSKIVVDCAADAHQATLRVTDEGP
GIPPDELPFLFDRFFRGAGARASENEGVGLGLALSQSIIHAQGGRIEAANGPDGGAVFTVRIPLAPREAGS

Sequences:

>Translated_471_residues
MLNFRQRLVLTHWTVIVVIVACSALAGWWQLSRLVHGQLDAALLTLADTEAGMQVESHGQPVRVHERPPGSAPPSLVRLD
RLVQIVNAKGEILARSSNLGDAMLPAPPALLDELASGGQAVFQTLPSFGEEPLRMVSLPAVANGKPVVIQVAGSLDDVHN
VLESATILFGIMAAALLAAVGAAGSVLTRRAFRGINDVVKQARLIGDANLGERLPHPGTTDEIGQLVDTLNAMLDRLEQA
FDQQRRFTADASHELRSPLSRLRTEIEVTLRRPRESQEYVDTLRSCLDEVGRLTRLIEELLMLARFDAGQDRQAGEPQAL
DALIAETLLRAGPAARERQVELVARSEAVGGAKVSHGSVGLVLANLVDNALKFSPQGSKIVVDCAADAHQATLRVTDEGP
GIPPDELPFLFDRFFRGAGARASENEGVGLGLALSQSIIHAQGGRIEAANGPDGGAVFTVRIPLAPREAGS
>Mature_471_residues
MLNFRQRLVLTHWTVIVVIVACSALAGWWQLSRLVHGQLDAALLTLADTEAGMQVESHGQPVRVHERPPGSAPPSLVRLD
RLVQIVNAKGEILARSSNLGDAMLPAPPALLDELASGGQAVFQTLPSFGEEPLRMVSLPAVANGKPVVIQVAGSLDDVHN
VLESATILFGIMAAALLAAVGAAGSVLTRRAFRGINDVVKQARLIGDANLGERLPHPGTTDEIGQLVDTLNAMLDRLEQA
FDQQRRFTADASHELRSPLSRLRTEIEVTLRRPRESQEYVDTLRSCLDEVGRLTRLIEELLMLARFDAGQDRQAGEPQAL
DALIAETLLRAGPAARERQVELVARSEAVGGAKVSHGSVGLVLANLVDNALKFSPQGSKIVVDCAADAHQATLRVTDEGP
GIPPDELPFLFDRFFRGAGARASENEGVGLGLALSQSIIHAQGGRIEAANGPDGGAVFTVRIPLAPREAGS

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=399, Percent_Identity=29.3233082706767, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788393, Length=256, Percent_Identity=33.59375, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1789403, Length=239, Percent_Identity=32.6359832635983, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1790346, Length=245, Percent_Identity=32.6530612244898, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=29.7297297297297, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788279, Length=372, Percent_Identity=24.7311827956989, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1786912, Length=213, Percent_Identity=32.8638497652582, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI145693157, Length=247, Percent_Identity=29.9595141700405, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787894, Length=263, Percent_Identity=28.5171102661597, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788549, Length=222, Percent_Identity=29.7297297297297, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790551, Length=273, Percent_Identity=31.5018315018315, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789808, Length=297, Percent_Identity=27.6094276094276, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790861, Length=311, Percent_Identity=28.9389067524116, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081816, Length=231, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788713, Length=251, Percent_Identity=26.2948207171315, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=239, Percent_Identity=26.7782426778243, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87082128, Length=319, Percent_Identity=24.7648902821317, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI48994928, Length=237, Percent_Identity=25.7383966244726, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50202; Mature: 50202

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFRQRLVLTHWTVIVVIVACSALAGWWQLSRLVHGQLDAALLTLADTEAGMQVESHGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCCCC
PVRVHERPPGSAPPSLVRLDRLVQIVNAKGEILARSSNLGDAMLPAPPALLDELASGGQA
CEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCHHH
VFQTLPSFGEEPLRMVSLPAVANGKPVVIQVAGSLDDVHNVLESATILFGIMAAALLAAV
HHHHHHHCCCCCHHEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAAGSVLTRRAFRGINDVVKQARLIGDANLGERLPHPGTTDEIGQLVDTLNAMLDRLEQA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FDQQRRFTADASHELRSPLSRLRTEIEVTLRRPRESQEYVDTLRSCLDEVGRLTRLIEEL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LMLARFDAGQDRQAGEPQALDALIAETLLRAGPAARERQVELVARSEAVGGAKVSHGSVG
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCCHH
LVLANLVDNALKFSPQGSKIVVDCAADAHQATLRVTDEGPGIPPDELPFLFDRFFRGAGA
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCC
RASENEGVGLGLALSQSIIHAQGGRIEAANGPDGGAVFTVRIPLAPREAGS
CCCCCCCCEEEHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MLNFRQRLVLTHWTVIVVIVACSALAGWWQLSRLVHGQLDAALLTLADTEAGMQVESHGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCCCC
PVRVHERPPGSAPPSLVRLDRLVQIVNAKGEILARSSNLGDAMLPAPPALLDELASGGQA
CEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCHHH
VFQTLPSFGEEPLRMVSLPAVANGKPVVIQVAGSLDDVHNVLESATILFGIMAAALLAAV
HHHHHHHCCCCCHHEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAAGSVLTRRAFRGINDVVKQARLIGDANLGERLPHPGTTDEIGQLVDTLNAMLDRLEQA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FDQQRRFTADASHELRSPLSRLRTEIEVTLRRPRESQEYVDTLRSCLDEVGRLTRLIEEL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LMLARFDAGQDRQAGEPQALDALIAETLLRAGPAARERQVELVARSEAVGGAKVSHGSVG
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCCHH
LVLANLVDNALKFSPQGSKIVVDCAADAHQATLRVTDEGPGIPPDELPFLFDRFFRGAGA
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCC
RASENEGVGLGLALSQSIIHAQGGRIEAANGPDGGAVFTVRIPLAPREAGS
CCCCCCCCEEEHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]