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The map label for this gene is nac [H]

Identifier: 94314198

GI number: 94314198

Start: 1979051

End: 1979977

Strand: Reverse

Name: nac [H]

Synonym: Rmet_5279

Alternate gene names: 94314198

Gene position: 1979977-1979051 (Counterclockwise)

Preceding gene: 94314201

Following gene: 94314197

Centisome position: 76.74

GC content: 69.26

Gene sequence:

>927_bases
ATGGAACTGCGGCAGTTGCGCTACTTCGTGCATGTGGTGGAGCTTGGCAGCATGGGGCAGGCGGCCCAGCGACTGGGCGT
CGTCACGTCCGCGTTGAGCCAGCAGATCAGCCGCCTCGAGAGCGAGCTATCCACGCGACTGCTGCAGCGCACGTCGAGCG
GCGTGGTGCCGACCGACGCGGGTCTGGCCTTCTGGCGTCAGGCGCAACTGGCGCTGCGCCACGCGGACGATGCCGCGCGC
GCGGCGCGGACGGCGCGGCTGTCGGGCCACGTCAGCGTGGGCATGGCACCGAGCACGCTGGCGGTGCTGGGGCCTCCGTT
CATGGTCGCGATGCGCGAGCGTTATCCCGATATCCGTCTTCATCTGGTCGAAAGCCTCTCCGGCAACCTGGCTTCGATGA
TTGGCGCGCGCCAGCTTGACCTGGCCGTGCTGTTCCAGCTCGAAGCGGGTCAGCGCTGGAGCACCACGCCATTGCTCGAA
GAGCGGCTTTTCCTGATCGCGCGCCCCGATCAGCCCGGCTTGCCAGCGGCAAATGCGGTGCGGCTCGAGGACATCGGCGA
CATCCCGCTGATTCTGCCCAGCAGCGCGCACGGTCTGCGTGCCGTGCTGAACGCGGCCGCGGCCCGTCACCAGCGTGCGC
TGAACGTGGTGGCCGAAATCGACGGGCTGGCCGTACTGATGGACGCCGTGCGCGCCGGCATTGGCGCGACGATTCAGCCT
GGCGCCGCCGTGGCGCGCCATCTGGGCGACCCGCTGGCGCTGATCGAGATCGCCGATGCCAGCGTCAGGCGTCCGAACCT
GCTGGTCAGCCTGTCGGACGACGAACTGTCGCCGGCCGGCCTGGCAGCGCGAGGCGTGCTCGAATCAGTGGCCCGCCAAC
TGGTCACGGCCGAACGCTGGCCCGGCGCCACCCTTCACAAATCCTGA

Upstream 100 bases:

>100_bases
CGCTGCACGGCGAGGACGATGTCGACATGCTCGACCATCGTGCCCACCCGGTCAACCCGCGTACAAGGCACAATACCGGG
GGCTTGTGACAGGAGAGGAA

Downstream 100 bases:

>100_bases
ACACCTCCCGCCGGCACGGGGCTGGTGCAGGCTTCGCCACCTTCGTAAAGTCTCAGGGATCAGGCATTTCAGAATTCCAT
AGTTCCCCGAGGAGACTCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MELRQLRYFVHVVELGSMGQAAQRLGVVTSALSQQISRLESELSTRLLQRTSSGVVPTDAGLAFWRQAQLALRHADDAAR
AARTARLSGHVSVGMAPSTLAVLGPPFMVAMRERYPDIRLHLVESLSGNLASMIGARQLDLAVLFQLEAGQRWSTTPLLE
ERLFLIARPDQPGLPAANAVRLEDIGDIPLILPSSAHGLRAVLNAAAARHQRALNVVAEIDGLAVLMDAVRAGIGATIQP
GAAVARHLGDPLALIEIADASVRRPNLLVSLSDDELSPAGLAARGVLESVARQLVTAERWPGATLHKS

Sequences:

>Translated_308_residues
MELRQLRYFVHVVELGSMGQAAQRLGVVTSALSQQISRLESELSTRLLQRTSSGVVPTDAGLAFWRQAQLALRHADDAAR
AARTARLSGHVSVGMAPSTLAVLGPPFMVAMRERYPDIRLHLVESLSGNLASMIGARQLDLAVLFQLEAGQRWSTTPLLE
ERLFLIARPDQPGLPAANAVRLEDIGDIPLILPSSAHGLRAVLNAAAARHQRALNVVAEIDGLAVLMDAVRAGIGATIQP
GAAVARHLGDPLALIEIADASVRRPNLLVSLSDDELSPAGLAARGVLESVARQLVTAERWPGATLHKS
>Mature_308_residues
MELRQLRYFVHVVELGSMGQAAQRLGVVTSALSQQISRLESELSTRLLQRTSSGVVPTDAGLAFWRQAQLALRHADDAAR
AARTARLSGHVSVGMAPSTLAVLGPPFMVAMRERYPDIRLHLVESLSGNLASMIGARQLDLAVLFQLEAGQRWSTTPLLE
ERLFLIARPDQPGLPAANAVRLEDIGDIPLILPSSAHGLRAVLNAAAARHQRALNVVAEIDGLAVLMDAVRAGIGATIQP
GAAVARHLGDPLALIEIADASVRRPNLLVSLSDDELSPAGLAARGVLESVARQLVTAERWPGATLHKS

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=251, Percent_Identity=36.6533864541833, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1788748, Length=288, Percent_Identity=31.5972222222222, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI145693105, Length=280, Percent_Identity=24.6428571428571, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI157672245, Length=249, Percent_Identity=30.1204819277108, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787601, Length=206, Percent_Identity=31.5533980582524, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=28.6274509803922, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787879, Length=293, Percent_Identity=29.0102389078498, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788887, Length=244, Percent_Identity=27.8688524590164, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789204, Length=240, Percent_Identity=24.5833333333333, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786401, Length=168, Percent_Identity=30.3571428571429, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367136, Length=239, Percent_Identity=25.5230125523013, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32843; Mature: 32843

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRQLRYFVHVVELGSMGQAAQRLGVVTSALSQQISRLESELSTRLLQRTSSGVVPTDA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
GLAFWRQAQLALRHADDAARAARTARLSGHVSVGMAPSTLAVLGPPFMVAMRERYPDIRL
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCEEECCCCCHHHHHCCHHHHHHHHHCCCHHH
HLVESLSGNLASMIGARQLDLAVLFQLEAGQRWSTTPLLEERLFLIARPDQPGLPAANAV
HHHHHHCCHHHHHHCCHHCCEEEEEEECCCCCCCCCHHHHHCEEEEECCCCCCCCCCCEE
RLEDIGDIPLILPSSAHGLRAVLNAAAARHQRALNVVAEIDGLAVLMDAVRAGIGATIQP
EECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GAAVARHLGDPLALIEIADASVRRPNLLVSLSDDELSPAGLAARGVLESVARQLVTAERW
HHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PGATLHKS
CCCCCCCC
>Mature Secondary Structure
MELRQLRYFVHVVELGSMGQAAQRLGVVTSALSQQISRLESELSTRLLQRTSSGVVPTDA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
GLAFWRQAQLALRHADDAARAARTARLSGHVSVGMAPSTLAVLGPPFMVAMRERYPDIRL
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCEEECCCCCHHHHHCCHHHHHHHHHCCCHHH
HLVESLSGNLASMIGARQLDLAVLFQLEAGQRWSTTPLLEERLFLIARPDQPGLPAANAV
HHHHHHCCHHHHHHCCHHCCEEEEEEECCCCCCCCCHHHHHCEEEEECCCCCCCCCCCEE
RLEDIGDIPLILPSSAHGLRAVLNAAAARHQRALNVVAEIDGLAVLMDAVRAGIGATIQP
EECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GAAVARHLGDPLALIEIADASVRRPNLLVSLSDDELSPAGLAARGVLESVARQLVTAERW
HHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PGATLHKS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]