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The map label for this gene is cspA [H]

Identifier: 94314090

GI number: 94314090

Start: 1868927

End: 1869307

Strand: Reverse

Name: cspA [H]

Synonym: Rmet_5171

Alternate gene names: 94314090

Gene position: 1869307-1868927 (Counterclockwise)

Preceding gene: 94314096

Following gene: 94314075

Centisome position: 72.45

GC content: 50.66

Gene sequence:

>381_bases
ATGTTCATTGACCGAGGTGCGGTGTACCTGTATAAGTATGATCGCAGGATCTTCAAGCGTAACCTCGAAGCGTTAGGTGT
TTACCTCGGCAGCACCACTTTCTCTCCATGTTTCCTCCTTGATCGTCATGCCTCGTCCGCTATTTCGGCACGGCGAGAAT
TTTTAAGGAATTTCTACATGGAAACCGGTACTGTAAAGTGGTTTAACGATTCAAAAGGTTTTGGTTTCATCACGCCGGAT
GCAGGCGGAGACGACCTGTTTGCCCATTTTTCCGAAGTGCAAGGTAATGGGTTCAAGTCGCTTCAGGAAGGCCAGAAAGT
GCGCTATGTGGCCGGCGTAGGCCAGAAGGGCCCCGCGGCGACCAAGATCGAACCGATCTAA

Upstream 100 bases:

>100_bases
CGACTGTAGTATGCGGCGTTGAAAAGCCAGGGGTTGTTTCGCCGAAGGTCCGTTCATTGCCTCGCGCCGCGCTTGCAAGA
TACGTATCGATTGTCGTGAG

Downstream 100 bases:

>100_bases
GGTAGCCCTCGAAGTTGCGCAAGAAGCCCTGCCGGGAGAAAACCGGCGGGGCTTTTTCTATTTCGTGACGACGAAATAGG
TGCGGCGCGATGGGTACAGA

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MFIDRGAVYLYKYDRRIFKRNLEALGVYLGSTTFSPCFLLDRHASSAISARREFLRNFYMETGTVKWFNDSKGFGFITPD
AGGDDLFAHFSEVQGNGFKSLQEGQKVRYVAGVGQKGPAATKIEPI

Sequences:

>Translated_126_residues
MFIDRGAVYLYKYDRRIFKRNLEALGVYLGSTTFSPCFLLDRHASSAISARREFLRNFYMETGTVKWFNDSKGFGFITPD
AGGDDLFAHFSEVQGNGFKSLQEGQKVRYVAGVGQKGPAATKIEPI
>Mature_126_residues
MFIDRGAVYLYKYDRRIFKRNLEALGVYLGSTTFSPCFLLDRHASSAISARREFLRNFYMETGTVKWFNDSKGFGFITPD
AGGDDLFAHFSEVQGNGFKSLQEGQKVRYVAGVGQKGPAATKIEPI

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=62.5, Blast_Score=86, Evalue=7e-19,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=58.4615384615385, Blast_Score=83, Evalue=7e-18,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=59.0163934426229, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=52.2388059701493, Blast_Score=81, Evalue=2e-17,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=56.4516129032258, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=56.4516129032258, Blast_Score=75, Evalue=9e-16,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=53.2258064516129, Blast_Score=73, Evalue=7e-15,

Paralogues:

None

Copy number: 8300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 14162; Mature: 14162

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIDRGAVYLYKYDRRIFKRNLEALGVYLGSTTFSPCFLLDRHASSAISARREFLRNFYM
CCCCCCEEEEEHHHHHHHHHHHHHHHHEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
ETGTVKWFNDSKGFGFITPDAGGDDLFAHFSEVQGNGFKSLQEGQKVRYVAGVGQKGPAA
CCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCCCCC
TKIEPI
CCCCCC
>Mature Secondary Structure
MFIDRGAVYLYKYDRRIFKRNLEALGVYLGSTTFSPCFLLDRHASSAISARREFLRNFYM
CCCCCCEEEEEHHHHHHHHHHHHHHHHEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
ETGTVKWFNDSKGFGFITPDAGGDDLFAHFSEVQGNGFKSLQEGQKVRYVAGVGQKGPAA
CCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCCCCC
TKIEPI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]