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The map label for this gene is hadH [H]

Identifier: 94314074

GI number: 94314074

Start: 1853717

End: 1854484

Strand: Reverse

Name: hadH [H]

Synonym: Rmet_5155

Alternate gene names: 94314074

Gene position: 1854484-1853717 (Counterclockwise)

Preceding gene: 94314075

Following gene: 94314073

Centisome position: 71.88

GC content: 67.06

Gene sequence:

>768_bases
ATGAAACTCGACCAGACCATGTCCGCCGTCGTGACCGGCGGTGCCTCCGGCCTCGGCCTTGCCAGCGCCAAACGCCTTGT
GGAGAAGGGTGTCAAGGTGGTGATTGCCGACATCTCGGAAGAACGTGCCGCTGCCGCGCTGGATGCACTGGGTCCCAATG
CCACGTTCGTCCAGGCCGACGTCACCGATACCGACCAGATGACGCGCGTGTTCGACGCTGCAGTCGCGATGGGGCCGCTG
CGTGCGCTAGTGCATTGCGCGGGGCTCGGTGGCGCGGTACGCGTGGTGGAAAAGGACGGCTCGCCGGGCTCGCTCGAAGC
CTATGAGCGCGTGGTGCGGATCAACCTGATTGGCACCTTCAACGTGCTGCGTCTGGGTGCGGCACGCATGGCGACCAACG
AGCCCGTCGACGGCGAGCGTGGCGCATGCGTGCTGACCGCGTCGGTGGCGGCCTACGAAGGCCAGATCGGCCAGATTCCT
TACGCATCGGCCAAGGCGGGCATCGTCGGCATGACCATCGTCGCGGCACGCGATCTGGCACAGCGCATGATTCGCGTCTG
CACGATCGCCCCGGGCCTGTTCGACACGCCGCTGCTGGCCCGTCTGCCCGATAACGTGAAGGCCTCGTTGGGTGCGATGG
TGCCGCACCCGGCCCGCCTGGGCGCGCCGGACGAGTACGCGTCCACCGCGCTGCACATCCTCGAGAATCCGATGCTCAAT
GGCGAGACCATCCGTCTGGATGGCGCCATCCGCATGGCCCCGCGCTAA

Upstream 100 bases:

>100_bases
GGCCTGCAGTCGATGTGCGAAGCGGGCGGCATGGCGAACGCCACGATCATCGAGCGCGTCTGACGCCCATCGCAATCCCA
TACGAACAGGAAAAACAAGC

Downstream 100 bases:

>100_bases
GGAGTCGATCATGACCGACACGCTAGTCAAGGAGATCCGCGGCAACGTGATGGTGCTGACCATTAACCGCCCCGAAGCGC
GCAATGCGATGGACTTCGAA

Product: short-chain dehydrogenase/reductase SDR

Products: 3-oxoacyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKLDQTMSAVVTGGASGLGLASAKRLVEKGVKVVIADISEERAAAALDALGPNATFVQADVTDTDQMTRVFDAAVAMGPL
RALVHCAGLGGAVRVVEKDGSPGSLEAYERVVRINLIGTFNVLRLGAARMATNEPVDGERGACVLTASVAAYEGQIGQIP
YASAKAGIVGMTIVAARDLAQRMIRVCTIAPGLFDTPLLARLPDNVKASLGAMVPHPARLGAPDEYASTALHILENPMLN
GETIRLDGAIRMAPR

Sequences:

>Translated_255_residues
MKLDQTMSAVVTGGASGLGLASAKRLVEKGVKVVIADISEERAAAALDALGPNATFVQADVTDTDQMTRVFDAAVAMGPL
RALVHCAGLGGAVRVVEKDGSPGSLEAYERVVRINLIGTFNVLRLGAARMATNEPVDGERGACVLTASVAAYEGQIGQIP
YASAKAGIVGMTIVAARDLAQRMIRVCTIAPGLFDTPLLARLPDNVKASLGAMVPHPARLGAPDEYASTALHILENPMLN
GETIRLDGAIRMAPR
>Mature_255_residues
MKLDQTMSAVVTGGASGLGLASAKRLVEKGVKVVIADISEERAAAALDALGPNATFVQADVTDTDQMTRVFDAAVAMGPL
RALVHCAGLGGAVRVVEKDGSPGSLEAYERVVRINLIGTFNVLRLGAARMATNEPVDGERGACVLTASVAAYEGQIGQIP
YASAKAGIVGMTIVAARDLAQRMIRVCTIAPGLFDTPLLARLPDNVKASLGAMVPHPARLGAPDEYASTALHILENPMLN
GETIRLDGAIRMAPR

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=251, Percent_Identity=51.792828685259, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI83715985, Length=251, Percent_Identity=49.003984063745, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI15277342, Length=263, Percent_Identity=27.7566539923954, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=25.7142857142857, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI31542939, Length=256, Percent_Identity=28.90625, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI59889578, Length=216, Percent_Identity=27.3148148148148, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI19923817, Length=260, Percent_Identity=26.5384615384615, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787335, Length=248, Percent_Identity=32.258064516129, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI2367175, Length=198, Percent_Identity=32.8282828282828, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789208, Length=245, Percent_Identity=28.1632653061224, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790717, Length=256, Percent_Identity=27.734375, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=256, Percent_Identity=44.140625, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI25147288, Length=252, Percent_Identity=30.5555555555556, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17555706, Length=251, Percent_Identity=30.2788844621514, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17561402, Length=255, Percent_Identity=25.8823529411765, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17737361, Length=249, Percent_Identity=48.5943775100402, Blast_Score=231, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24639444, Length=254, Percent_Identity=31.1023622047244, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355319, Length=249, Percent_Identity=27.710843373494, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.35

Molecular weight: Translated: 26468; Mature: 26468

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDQTMSAVVTGGASGLGLASAKRLVEKGVKVVIADISEERAAAALDALGPNATFVQAD
CCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCEEEEEC
VTDTDQMTRVFDAAVAMGPLRALVHCAGLGGAVRVVEKDGSPGSLEAYERVVRINLIGTF
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHEEEEEEEEHH
NVLRLGAARMATNEPVDGERGACVLTASVAAYEGQIGQIPYASAKAGIVGMTIVAARDLA
HHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QRMIRVCTIAPGLFDTPLLARLPDNVKASLGAMVPHPARLGAPDEYASTALHILENPMLN
HHHHHHHHCCCCCCCCCHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GETIRLDGAIRMAPR
CCEEEECCEEEECCC
>Mature Secondary Structure
MKLDQTMSAVVTGGASGLGLASAKRLVEKGVKVVIADISEERAAAALDALGPNATFVQAD
CCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCEEEEEC
VTDTDQMTRVFDAAVAMGPLRALVHCAGLGGAVRVVEKDGSPGSLEAYERVVRINLIGTF
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHEEEEEEEEHH
NVLRLGAARMATNEPVDGERGACVLTASVAAYEGQIGQIPYASAKAGIVGMTIVAARDLA
HHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QRMIRVCTIAPGLFDTPLLARLPDNVKASLGAMVPHPARLGAPDEYASTALHILENPMLN
HHHHHHHHCCCCCCCCCHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GETIRLDGAIRMAPR
CCEEEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-3-hydroxyacyl-CoA; NAD+

Specific reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]