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The map label for this gene is caiD [H]

Identifier: 94314073

GI number: 94314073

Start: 1852942

End: 1853706

Strand: Reverse

Name: caiD [H]

Synonym: Rmet_5154

Alternate gene names: 94314073

Gene position: 1853706-1852942 (Counterclockwise)

Preceding gene: 94314074

Following gene: 94314072

Centisome position: 71.85

GC content: 67.32

Gene sequence:

>765_bases
ATGACCGACACGCTAGTCAAGGAGATCCGCGGCAACGTGATGGTGCTGACCATTAACCGCCCCGAAGCGCGCAATGCGAT
GGACTTCGAAACGGCCACCGCCATTGCGGCAGCGCTGGACGAACTCGATGCGCGCGACGACCTGAGCATCGCGGTGCTGA
CCGGTGCCGGCAACACCTTCTGTTCCGGCATGGACCTGAAGGGGTTCCTCGCCGGCAAGCGGCCCAGCATTCCGGGGCGC
GGCTTTGGTGGCGTGACCGAGCGCCCGCCGCGCAAGGTGTTGATCGCCGCGGTTGAGGGCTACGCGCTGGCGGGTGGCTT
CGAACTCGTGCTCGCTTGCGATCTGGTCGTGGCCTCGCGCGAAGCAAAGTTCGGCCTGCCAGAAGTCAAGCGTGGCCTGG
CTGCAGCAGCCGGCGGCCTGATGCGCTTGCCGCGCCGCGTGCCGTACCACGTCGCCATGGAGTACAGCCTGACCGGCGAC
ATGCTGCCGGCCACGCGTGCCTACGAACTGGGTCTCGTCAATCGCTTGGCCGAAGCTGGCGGCGCGCTGGATGCCGCGCT
GCAACTGGCCGCTGCGGTCGGCGCCAACGGGCCGCTGGCGATCGCCGCGAGCAAGCAGGTGGTGGTGCAATCGGGCGACT
GGACCCGCGAAGAAATGTTCGAGCGTCAACGGCCGCTGATCGAGTCGGTTTTCACCTCGGCCGACGCGCGTGAAGGCGCG
GCGGCATTTGCGGAGAAGCGCAAGCCGGTCTGGACCGGCAAGTAA

Upstream 100 bases:

>100_bases
GCGTCCACCGCGCTGCACATCCTCGAGAATCCGATGCTCAATGGCGAGACCATCCGTCTGGATGGCGCCATCCGCATGGC
CCCGCGCTAAGGAGTCGATC

Downstream 100 bases:

>100_bases
CACCCTGTCTGGATTGGCCGCCCCGAGCCTGATCAGGGGCGGCCTTTCGCGGCTGCAGCACCCGCGATTACAGACCCGTC
GCAGCAAGAAAGACATGCGC

Product: enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTDTLVKEIRGNVMVLTINRPEARNAMDFETATAIAAALDELDARDDLSIAVLTGAGNTFCSGMDLKGFLAGKRPSIPGR
GFGGVTERPPRKVLIAAVEGYALAGGFELVLACDLVVASREAKFGLPEVKRGLAAAAGGLMRLPRRVPYHVAMEYSLTGD
MLPATRAYELGLVNRLAEAGGALDAALQLAAAVGANGPLAIAASKQVVVQSGDWTREEMFERQRPLIESVFTSADAREGA
AAFAEKRKPVWTGK

Sequences:

>Translated_254_residues
MTDTLVKEIRGNVMVLTINRPEARNAMDFETATAIAAALDELDARDDLSIAVLTGAGNTFCSGMDLKGFLAGKRPSIPGR
GFGGVTERPPRKVLIAAVEGYALAGGFELVLACDLVVASREAKFGLPEVKRGLAAAAGGLMRLPRRVPYHVAMEYSLTGD
MLPATRAYELGLVNRLAEAGGALDAALQLAAAVGANGPLAIAASKQVVVQSGDWTREEMFERQRPLIESVFTSADAREGA
AAFAEKRKPVWTGK
>Mature_253_residues
TDTLVKEIRGNVMVLTINRPEARNAMDFETATAIAAALDELDARDDLSIAVLTGAGNTFCSGMDLKGFLAGKRPSIPGRG
FGGVTERPPRKVLIAAVEGYALAGGFELVLACDLVVASREAKFGLPEVKRGLAAAAGGLMRLPRRVPYHVAMEYSLTGDM
LPATRAYELGLVNRLAEAGGALDAALQLAAAVGANGPLAIAASKQVVVQSGDWTREEMFERQRPLIESVFTSADAREGAA
AFAEKRKPVWTGK

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=253, Percent_Identity=32.4110671936759, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI31542718, Length=261, Percent_Identity=27.2030651340996, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI70995211, Length=261, Percent_Identity=30.2681992337165, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4502327, Length=265, Percent_Identity=30.188679245283, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI68989263, Length=190, Percent_Identity=30.5263157894737, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI150378541, Length=253, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI25453481, Length=253, Percent_Identity=23.3201581027668, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI51538573, Length=253, Percent_Identity=23.3201581027668, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=40, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=34.7328244274809, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=32.1969696969697, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=27.8884462151394, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=33.8308457711443, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788597, Length=242, Percent_Identity=28.5123966942149, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1790281, Length=214, Percent_Identity=28.9719626168224, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=34.0080971659919, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=35.8024691358025, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=32.4110671936759, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=37.0731707317073, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=231, Percent_Identity=27.2727272727273, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=267, Percent_Identity=25.0936329588015, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=187, Percent_Identity=28.3422459893048, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=198, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=198, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=198, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=243, Percent_Identity=26.3374485596708, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=36.734693877551, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=36.734693877551, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI21357171, Length=256, Percent_Identity=31.640625, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24653139, Length=274, Percent_Identity=34.3065693430657, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24650670, Length=202, Percent_Identity=34.6534653465347, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=28.6821705426357, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920382, Length=260, Percent_Identity=27.3076923076923, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921000, Length=186, Percent_Identity=31.1827956989247, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583077, Length=186, Percent_Identity=31.1827956989247, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583079, Length=186, Percent_Identity=31.1827956989247, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24654903, Length=185, Percent_Identity=27.027027027027, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 26815; Mature: 26684

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTLVKEIRGNVMVLTINRPEARNAMDFETATAIAAALDELDARDDLSIAVLTGAGNTF
CCHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCE
CSGMDLKGFLAGKRPSIPGRGFGGVTERPPRKVLIAAVEGYALAGGFELVLACDLVVASR
ECCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCHHEEHHHHHHHHCC
EAKFGLPEVKRGLAAAAGGLMRLPRRVPYHVAMEYSLTGDMLPATRAYELGLVNRLAEAG
CCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHC
GALDAALQLAAAVGANGPLAIAASKQVVVQSGDWTREEMFERQRPLIESVFTSADAREGA
CHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
AAFAEKRKPVWTGK
HHHHHCCCCCCCCC
>Mature Secondary Structure 
TDTLVKEIRGNVMVLTINRPEARNAMDFETATAIAAALDELDARDDLSIAVLTGAGNTF
CHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCE
CSGMDLKGFLAGKRPSIPGRGFGGVTERPPRKVLIAAVEGYALAGGFELVLACDLVVASR
ECCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCHHEEHHHHHHHHCC
EAKFGLPEVKRGLAAAAGGLMRLPRRVPYHVAMEYSLTGDMLPATRAYELGLVNRLAEAG
CCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHC
GALDAALQLAAAVGANGPLAIAASKQVVVQSGDWTREEMFERQRPLIESVFTSADAREGA
CHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
AAFAEKRKPVWTGK
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA