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The map label for this gene is phoQ [H]

Identifier: 94314048

GI number: 94314048

Start: 1826280

End: 1827626

Strand: Reverse

Name: phoQ [H]

Synonym: Rmet_5129

Alternate gene names: 94314048

Gene position: 1827626-1826280 (Counterclockwise)

Preceding gene: 94314049

Following gene: 94314047

Centisome position: 70.84

GC content: 66.74

Gene sequence:

>1347_bases
ATGAAGCGGCCCACCATCGCGGGCAGGTTGCTGCTGATTGCCACGCTGTGGGTGGTCGGCACGTTATGCGCCACGGGCTG
GGTGCTCGATACGCTGTTCGGGCGTCACGTCGACAAGACATATGTGACCGAGTTGGACCGGGACCTGACCAGCCTTGCCG
CCGCGCTCGAATGGAATGGCCACGGCCAGCTGGAACTGACGCGCCAGCCCGCCGATCCGCGCTTTGAACGGCCATACTCG
GGCGCCTACTGGCAGGCCGCGTCGGGCACGCAATCGCTACGGTCGCGGTCGCTGTGGGACATCGACATCCCCACGACGAT
CGAGCGCAAGATTCCGGCGGGAGACGCCGATATCCGGAACGGCCCGGAAGGCCAGTCGCTGCTGGCGAAGTCCCGCAGGC
TGCAATTGACCGGGGATGACGCGTACGTCCAGATCACGGTGGCACTGGATCGCACCGAATTGCGGGACGCCAAACGGTCG
TTCCGGCATCTGTTGTGGCTGTCACTCGGCATCCTTGGTGTGGGCATTCTCGGCGCCGTCTGGGCGCAGATCCGCTTTGG
GCTGGCGCCGCTGAGCCGCCTGCGCGCGGCGGTGGCAAAGCTGCACGGCGGTGGCGACCCCACGTTGCAGGGGGTATGGC
CGGCCGAAGTGAAGCCTCTGGTCGATGAGATCAACGTGCTTCTCAAACGCAATCACGATGCCGTGGAGCGGTCGCGCCGG
CAGGCGGCGGATCTGGCCCACGCGGTCAAGACGCCGCTGGCGATCCTGGCCAACAGCGCCACGGCCTTGCCCGGTCCGCT
CTCCCAGAGCGTGCTGCAGCAGGTGGACGCGATGCGCCAGCAGGTGGATCGGCATCTGTCGCGGGCCCGCGCGGTGGGTG
CCGCCGGCGCGGGCGGGCGAAGTGTTGCGATCCGCCCGGCGGTCGAGGAACTCGCCCGCGCGCTCGTGCGGCTTCATGCG
GGCAAGCCGATTGAAGTCAGTGTGGAAGGCAATGCCGACTTCTTTGGTGACAGGCAGGATCTTGTCGAGATGCTCGGCAA
CCTGCTGGACAACGCCTGGAAATGGGCGAGCACGCGTATCCGCGTGACGCTGGTATCGCAGGAGTCGGAGATCGAGATCT
GCGTCGACGACGATGGCCCGGGCATGCCCGCAAGCTCCATGCATCAGGCCATGCAACCCTTCTCCCGGCTGGACGAATCC
GTCGAGGGCAGTGGACTGGGCCTGTCTATCGTGCGCGATATCTGCGCGATGTACGACGGGGCGTTCGAACTGAAGCGCTC
CTCTCTGGGCGGTCTGGCCGCGCGGATGCGGTTTCCGGCCGCAGGTGCGACGCAGTCGCAGCGCTGA

Upstream 100 bases:

>100_bases
TCGAGGTATTCGTCGGTCGCCTGCGACGCAAGCTCGGTGTCGACGTCATCGAAACGGTGCGTGGCCTCGGATACCGGCTG
CGCCCGCCGGAACACGGCGC

Downstream 100 bases:

>100_bases
GCCGCCGAGCCGCTGACCGGCCAGTGCCGTAGCCACCGACTGTTGATCCCTGGAACGGCCGGTGTTCCATTCTTGAACAG
TCTTTCTCGTTGCCACCGGG

Product: putative two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKRPTIAGRLLLIATLWVVGTLCATGWVLDTLFGRHVDKTYVTELDRDLTSLAAALEWNGHGQLELTRQPADPRFERPYS
GAYWQAASGTQSLRSRSLWDIDIPTTIERKIPAGDADIRNGPEGQSLLAKSRRLQLTGDDAYVQITVALDRTELRDAKRS
FRHLLWLSLGILGVGILGAVWAQIRFGLAPLSRLRAAVAKLHGGGDPTLQGVWPAEVKPLVDEINVLLKRNHDAVERSRR
QAADLAHAVKTPLAILANSATALPGPLSQSVLQQVDAMRQQVDRHLSRARAVGAAGAGGRSVAIRPAVEELARALVRLHA
GKPIEVSVEGNADFFGDRQDLVEMLGNLLDNAWKWASTRIRVTLVSQESEIEICVDDDGPGMPASSMHQAMQPFSRLDES
VEGSGLGLSIVRDICAMYDGAFELKRSSLGGLAARMRFPAAGATQSQR

Sequences:

>Translated_448_residues
MKRPTIAGRLLLIATLWVVGTLCATGWVLDTLFGRHVDKTYVTELDRDLTSLAAALEWNGHGQLELTRQPADPRFERPYS
GAYWQAASGTQSLRSRSLWDIDIPTTIERKIPAGDADIRNGPEGQSLLAKSRRLQLTGDDAYVQITVALDRTELRDAKRS
FRHLLWLSLGILGVGILGAVWAQIRFGLAPLSRLRAAVAKLHGGGDPTLQGVWPAEVKPLVDEINVLLKRNHDAVERSRR
QAADLAHAVKTPLAILANSATALPGPLSQSVLQQVDAMRQQVDRHLSRARAVGAAGAGGRSVAIRPAVEELARALVRLHA
GKPIEVSVEGNADFFGDRQDLVEMLGNLLDNAWKWASTRIRVTLVSQESEIEICVDDDGPGMPASSMHQAMQPFSRLDES
VEGSGLGLSIVRDICAMYDGAFELKRSSLGGLAARMRFPAAGATQSQR
>Mature_448_residues
MKRPTIAGRLLLIATLWVVGTLCATGWVLDTLFGRHVDKTYVTELDRDLTSLAAALEWNGHGQLELTRQPADPRFERPYS
GAYWQAASGTQSLRSRSLWDIDIPTTIERKIPAGDADIRNGPEGQSLLAKSRRLQLTGDDAYVQITVALDRTELRDAKRS
FRHLLWLSLGILGVGILGAVWAQIRFGLAPLSRLRAAVAKLHGGGDPTLQGVWPAEVKPLVDEINVLLKRNHDAVERSRR
QAADLAHAVKTPLAILANSATALPGPLSQSVLQQVDAMRQQVDRHLSRARAVGAAGAGGRSVAIRPAVEELARALVRLHA
GKPIEVSVEGNADFFGDRQDLVEMLGNLLDNAWKWASTRIRVTLVSQESEIEICVDDDGPGMPASSMHQAMQPFSRLDES
VEGSGLGLSIVRDICAMYDGAFELKRSSLGGLAARMRFPAAGATQSQR

Specific function: Member of the two-component regulatory system phoQ/phoP involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane- associated p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787374, Length=269, Percent_Identity=29.368029739777, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790551, Length=309, Percent_Identity=25.5663430420712, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=24.7232472324723, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788279, Length=256, Percent_Identity=28.90625, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790346, Length=117, Percent_Identity=35.042735042735, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790861, Length=306, Percent_Identity=26.797385620915, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787894, Length=221, Percent_Identity=28.0542986425339, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789808, Length=295, Percent_Identity=25.4237288135593, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789403, Length=268, Percent_Identity=27.9850746268657, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48694; Mature: 48694

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPTIAGRLLLIATLWVVGTLCATGWVLDTLFGRHVDKTYVTELDRDLTSLAAALEWNG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECC
HGQLELTRQPADPRFERPYSGAYWQAASGTQSLRSRSLWDIDIPTTIERKIPAGDADIRN
CCCEEEECCCCCCCCCCCCCCCCEECCCCHHHHHHCCCEECCCCCHHHCCCCCCCCCCCC
GPEGQSLLAKSRRLQLTGDDAYVQITVALDRTELRDAKRSFRHLLWLSLGILGVGILGAV
CCCHHHHHHHCCCEEEECCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WAQIRFGLAPLSRLRAAVAKLHGGGDPTLQGVWPAEVKPLVDEINVLLKRNHDAVERSRR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
QAADLAHAVKTPLAILANSATALPGPLSQSVLQQVDAMRQQVDRHLSRARAVGAAGAGGR
HHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SVAIRPAVEELARALVRLHAGKPIEVSVEGNADFFGDRQDLVEMLGNLLDNAWKWASTRI
EEEECHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
RVTLVSQESEIEICVDDDGPGMPASSMHQAMQPFSRLDESVEGSGLGLSIVRDICAMYDG
EEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
AFELKRSSLGGLAARMRFPAAGATQSQR
HHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKRPTIAGRLLLIATLWVVGTLCATGWVLDTLFGRHVDKTYVTELDRDLTSLAAALEWNG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECC
HGQLELTRQPADPRFERPYSGAYWQAASGTQSLRSRSLWDIDIPTTIERKIPAGDADIRN
CCCEEEECCCCCCCCCCCCCCCCEECCCCHHHHHHCCCEECCCCCHHHCCCCCCCCCCCC
GPEGQSLLAKSRRLQLTGDDAYVQITVALDRTELRDAKRSFRHLLWLSLGILGVGILGAV
CCCHHHHHHHCCCEEEECCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WAQIRFGLAPLSRLRAAVAKLHGGGDPTLQGVWPAEVKPLVDEINVLLKRNHDAVERSRR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
QAADLAHAVKTPLAILANSATALPGPLSQSVLQQVDAMRQQVDRHLSRARAVGAAGAGGR
HHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SVAIRPAVEELARALVRLHAGKPIEVSVEGNADFFGDRQDLVEMLGNLLDNAWKWASTRI
EEEECHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
RVTLVSQESEIEICVDDDGPGMPASSMHQAMQPFSRLDESVEGSGLGLSIVRDICAMYDG
EEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
AFELKRSSLGGLAARMRFPAAGATQSQR
HHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]