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The map label for this gene is echA8 [H]

Identifier: 94314026

GI number: 94314026

Start: 1799366

End: 1800142

Strand: Reverse

Name: echA8 [H]

Synonym: Rmet_5107

Alternate gene names: 94314026

Gene position: 1800142-1799366 (Counterclockwise)

Preceding gene: 94314027

Following gene: 94314022

Centisome position: 69.77

GC content: 66.15

Gene sequence:

>777_bases
ATGAGCGCATCGCTTTCCAATCACCACGCCGCCGTAGAGATCGACGAGCGCGGCGTAGCCACCGTCACCATTCGCGAGGC
GAAATCGCTGAATATCCTCGGGACGCCGGTCATTGCGGATCTGACCCAGGCCGTGCGCGGCCTGGCCGAACGCGACGACG
TGCGCGTGCTGGTGGTTCGCGGCACGGGGGACAAGGGCTTTATCGGGGGCGCCGATATCAACGAGATGGCCGCGCTGACC
CGCGAGCGCGCGGAAGTGTTCATCAGCGCATTGCGCGACTTGTGCAATGCGCTGCGTCACTTCCCGGTACCCGTGATTGC
GCGAATGCCGGGCTGGTGCCTCGGCGGCGGCTTGGAACTGGCGCTGGCGTGCGATATCCGCATCGCCGCCGACAACGCCC
AGCTGGGTATGCCGGAGGTCAAGGTAGGCATCCCGTCGGTCATTCATGCGGCGCTGATGCCGCGCCTGATTGGCAACGCG
CGCGCCGCGTGGTTGTTGCTGACGGGCGAGATCGCCGACGCGGCAGAGTCGCTGGCTGCGGGGATAGTGAGCCGCGTGGT
GCCGCTGGCCGATCTCGACGTCGAGGTTAGTCGTGTGGCATCACTGCTGGCGAGCTTCGGCCCGCAGGTGGTGCGTCAGC
AGAAGCGTCTGCTGCGCGAGTGGGAAGAAGCGCCGCTCGACGTGTCGATCGACAACAGTGTGGCGGAGTTCGGCAGCGCC
TTCGATACGGGCGAGCCGCAGCATCACATGGCCTCCTTCCTGAACCGCAAGCGGTAA

Upstream 100 bases:

>100_bases
CGCAAGGCCTTCGCCGATCTGATACGGGAGCAGAATCAGCACTGGAAGAAGGTAGTCGAGGATATCCATTTCGCAAAGCT
GCAGTAAGGAGCAGAACACG

Downstream 100 bases:

>100_bases
CAGGAATCAGGACGCGGCGTCAGGGGCCGCGCCTGTCTCGCGTGTGATGGCCACGCGCGGGTCGTTGAGCAGGAACTGCA
CGAACGCCGCGGCAAAGCCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSASLSNHHAAVEIDERGVATVTIREAKSLNILGTPVIADLTQAVRGLAERDDVRVLVVRGTGDKGFIGGADINEMAALT
RERAEVFISALRDLCNALRHFPVPVIARMPGWCLGGGLELALACDIRIAADNAQLGMPEVKVGIPSVIHAALMPRLIGNA
RAAWLLLTGEIADAAESLAAGIVSRVVPLADLDVEVSRVASLLASFGPQVVRQQKRLLREWEEAPLDVSIDNSVAEFGSA
FDTGEPQHHMASFLNRKR

Sequences:

>Translated_258_residues
MSASLSNHHAAVEIDERGVATVTIREAKSLNILGTPVIADLTQAVRGLAERDDVRVLVVRGTGDKGFIGGADINEMAALT
RERAEVFISALRDLCNALRHFPVPVIARMPGWCLGGGLELALACDIRIAADNAQLGMPEVKVGIPSVIHAALMPRLIGNA
RAAWLLLTGEIADAAESLAAGIVSRVVPLADLDVEVSRVASLLASFGPQVVRQQKRLLREWEEAPLDVSIDNSVAEFGSA
FDTGEPQHHMASFLNRKR
>Mature_257_residues
SASLSNHHAAVEIDERGVATVTIREAKSLNILGTPVIADLTQAVRGLAERDDVRVLVVRGTGDKGFIGGADINEMAALTR
ERAEVFISALRDLCNALRHFPVPVIARMPGWCLGGGLELALACDIRIAADNAQLGMPEVKVGIPSVIHAALMPRLIGNAR
AAWLLLTGEIADAAESLAAGIVSRVVPLADLDVEVSRVASLLASFGPQVVRQQKRLLREWEEAPLDVSIDNSVAEFGSAF
DTGEPQHHMASFLNRKR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=241, Percent_Identity=31.5352697095436, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI31542718, Length=220, Percent_Identity=31.3636363636364, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4502327, Length=200, Percent_Identity=35, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=29.5698924731183, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI68989263, Length=159, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=30.8988764044944, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260274832, Length=251, Percent_Identity=24.3027888446215, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI45643119, Length=251, Percent_Identity=24.3027888446215, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260275230, Length=251, Percent_Identity=24.3027888446215, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI37594471, Length=191, Percent_Identity=27.2251308900524, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI37594469, Length=191, Percent_Identity=27.2251308900524, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI213417737, Length=236, Percent_Identity=23.728813559322, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157694516, Length=227, Percent_Identity=24.2290748898678, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=244, Percent_Identity=29.5081967213115, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788597, Length=198, Percent_Identity=30.8080808080808, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788682, Length=181, Percent_Identity=34.8066298342541, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=34.0909090909091, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI221142681, Length=180, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082183, Length=245, Percent_Identity=26.530612244898, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787660, Length=224, Percent_Identity=31.25, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=240, Percent_Identity=32.9166666666667, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=173, Percent_Identity=32.9479768786127, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=28.9795918367347, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=235, Percent_Identity=28.0851063829787, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=193, Percent_Identity=32.1243523316062, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=189, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=174, Percent_Identity=32.183908045977, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=174, Percent_Identity=32.183908045977, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=210, Percent_Identity=25.2380952380952, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=249, Percent_Identity=26.9076305220884, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=174, Percent_Identity=32.183908045977, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=186, Percent_Identity=26.8817204301075, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=201, Percent_Identity=25.3731343283582, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=27.9693486590038, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=27.9693486590038, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653139, Length=249, Percent_Identity=27.3092369477912, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922422, Length=243, Percent_Identity=22.6337448559671, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920382, Length=215, Percent_Identity=29.3023255813954, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357171, Length=199, Percent_Identity=29.1457286432161, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19921000, Length=269, Percent_Identity=28.2527881040892, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583077, Length=269, Percent_Identity=28.2527881040892, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24583079, Length=269, Percent_Identity=28.2527881040892, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24650670, Length=246, Percent_Identity=26.4227642276423, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27575; Mature: 27444

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASLSNHHAAVEIDERGVATVTIREAKSLNILGTPVIADLTQAVRGLAERDDVRVLVVR
CCCCCCCCCEEEEECCCCCEEEEEECCCCCCEECCHHHHHHHHHHHHHHCCCCEEEEEEE
GTGDKGFIGGADINEMAALTRERAEVFISALRDLCNALRHFPVPVIARMPGWCLGGGLEL
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEECCCCEE
ALACDIRIAADNAQLGMPEVKVGIPSVIHAALMPRLIGNARAAWLLLTGEIADAAESLAA
EEEEEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHH
GIVSRVVPLADLDVEVSRVASLLASFGPQVVRQQKRLLREWEEAPLDVSIDNSVAEFGSA
HHHHHHCCHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCC
FDTGEPQHHMASFLNRKR
CCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SASLSNHHAAVEIDERGVATVTIREAKSLNILGTPVIADLTQAVRGLAERDDVRVLVVR
CCCCCCCCEEEEECCCCCEEEEEECCCCCCEECCHHHHHHHHHHHHHHCCCCEEEEEEE
GTGDKGFIGGADINEMAALTRERAEVFISALRDLCNALRHFPVPVIARMPGWCLGGGLEL
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEECCCCEE
ALACDIRIAADNAQLGMPEVKVGIPSVIHAALMPRLIGNARAAWLLLTGEIADAAESLAA
EEEEEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHH
GIVSRVVPLADLDVEVSRVASLLASFGPQVVRQQKRLLREWEEAPLDVSIDNSVAEFGSA
HHHHHHCCHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCC
FDTGEPQHHMASFLNRKR
CCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]