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The map label for this gene is tcmN [H]

Identifier: 94313991

GI number: 94313991

Start: 1758701

End: 1759810

Strand: Direct

Name: tcmN [H]

Synonym: Rmet_5070

Alternate gene names: 94313991

Gene position: 1758701-1759810 (Clockwise)

Preceding gene: 94313983

Following gene: 94313992

Centisome position: 68.16

GC content: 62.07

Gene sequence:

>1110_bases
ATGACGTTCCAGATGAACGCAGGTGCCGTGCGCCGATTCGCCAAGGCCATGAGGTTCGGCGCCTGGCTTCAATCGATCAC
TGAGAGAATGACGCCAGCACCTTTCCGGCTGGTGCAGATCGGCTCGGCCTACTGGCAATCGAAAGCGCTCTACGTGGCGG
CGAAGCTGGACCTGGCGACTGTCCTGGGAACGGCAACCCTGACGGCATCTGCGCTGGCCAGCCGGGTCGGCGCCAATGAA
GATGCGCTCGGACGCCTGATGCGATTGCTTGCAGCCATGGGCATCTTCGAGGAAACGGCCCCCATGGTCTTCCGCAACAA
CAAGCTCTCCCGGTGCCTGGTGAGTGACGATCCCCAAAGCGTCCGGGCCATGATCCTGATGCACAACTCGGAAACCATGA
GCCGCCCATGGTTCGAGCAACTGGAGGCCGGCATCCGCAGTGGCACGCCGCCGTTTCAACTCAGCCACGGCGAGGACCTG
TTTGATTACCTGGACCATCACGCCGATTTCGACCAGCTTTTCTCGGAAGCCATGAACAGCGTCGAGGCACTGGCTGGCGA
CGGCTTTGCCACGGATCTGGATTGGGCCCGGTTCGACCGCATCATCGACGTTGGCGGATCACGGGGCACCAAGTCGCTGG
CGATCCTGCGACGCCATCCCAGGTTGACGGCACTGATCGTGGATCGGCCCCAGGTCATCGAGGAAGCCCAACGCTACTGG
GCAGAGCACCATGCGGATGGCCTCGAACGCCTGCAATTTCAGGCTGCGGATCTGTTTGAAAGCCTGCCTGCAGCAGGTCC
GAAGGATGTCTACCTGTACTCGGCCGTCCTGCATGGCTTTGACGACCCTACCTGCGTGCAGGCATTACTACGGCTGCGCG
AAGCCATCGGCAACAGCGGCGCCCGCGCGGCAATCCTGGAGATCGTTGTTCCGGAAAAAGGCGCCGACATCTCCAGCGCA
TCGTTCGACATGCAGATGTTCGTCGGCAGCCGGGGGCGCGAACGCACCTTGACCGAATGGAAAGCCATCATTCAAGCCGG
CGGCCTGACGCTTGAAGAGGTCGTGCGTCTCCGGTCGCTCGGGAGCATTCTGGTGTTGCGTGCGGGGTGA

Upstream 100 bases:

>100_bases
CGCATCCGCTTGATCAAGGCCGCGCTGGATGGCGTCCTCGCCGACGACTATCACCACGTCACGACCCACCGCATTGCCCA
ATTCGCCAAGGAGAATGAGC

Downstream 100 bases:

>100_bases
AGAGGACGGTTGCGTTGAGAATGTCGAGCTGGTGCGTTTGGGTCCCCGACGCTACGGAATTCGGATTGAGTACGAAGAAA
GCGCACTGATGTACTGCACC

Product: O-methyltransferase, family 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTFQMNAGAVRRFAKAMRFGAWLQSITERMTPAPFRLVQIGSAYWQSKALYVAAKLDLATVLGTATLTASALASRVGANE
DALGRLMRLLAAMGIFEETAPMVFRNNKLSRCLVSDDPQSVRAMILMHNSETMSRPWFEQLEAGIRSGTPPFQLSHGEDL
FDYLDHHADFDQLFSEAMNSVEALAGDGFATDLDWARFDRIIDVGGSRGTKSLAILRRHPRLTALIVDRPQVIEEAQRYW
AEHHADGLERLQFQAADLFESLPAAGPKDVYLYSAVLHGFDDPTCVQALLRLREAIGNSGARAAILEIVVPEKGADISSA
SFDMQMFVGSRGRERTLTEWKAIIQAGGLTLEEVVRLRSLGSILVLRAG

Sequences:

>Translated_369_residues
MTFQMNAGAVRRFAKAMRFGAWLQSITERMTPAPFRLVQIGSAYWQSKALYVAAKLDLATVLGTATLTASALASRVGANE
DALGRLMRLLAAMGIFEETAPMVFRNNKLSRCLVSDDPQSVRAMILMHNSETMSRPWFEQLEAGIRSGTPPFQLSHGEDL
FDYLDHHADFDQLFSEAMNSVEALAGDGFATDLDWARFDRIIDVGGSRGTKSLAILRRHPRLTALIVDRPQVIEEAQRYW
AEHHADGLERLQFQAADLFESLPAAGPKDVYLYSAVLHGFDDPTCVQALLRLREAIGNSGARAAILEIVVPEKGADISSA
SFDMQMFVGSRGRERTLTEWKAIIQAGGLTLEEVVRLRSLGSILVLRAG
>Mature_368_residues
TFQMNAGAVRRFAKAMRFGAWLQSITERMTPAPFRLVQIGSAYWQSKALYVAAKLDLATVLGTATLTASALASRVGANED
ALGRLMRLLAAMGIFEETAPMVFRNNKLSRCLVSDDPQSVRAMILMHNSETMSRPWFEQLEAGIRSGTPPFQLSHGEDLF
DYLDHHADFDQLFSEAMNSVEALAGDGFATDLDWARFDRIIDVGGSRGTKSLAILRRHPRLTALIVDRPQVIEEAQRYWA
EHHADGLERLQFQAADLFESLPAAGPKDVYLYSAVLHGFDDPTCVQALLRLREAIGNSGARAAILEIVVPEKGADISSAS
FDMQMFVGSRGRERTLTEWKAIIQAGGLTLEEVVRLRSLGSILVLRAG

Specific function: The N-terminal domain enhances the formation of an early, partially cyclized intermediate, while the C-terminal domain catalyzes the 3-O-methylation of one or more later intermediates in the biosynthetic pathway. Catalyzes the methylation of tetracenomyci

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI117553627, Length=366, Percent_Identity=28.6885245901639, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI291045181, Length=366, Percent_Identity=28.6885245901639, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI291045183, Length=366, Percent_Identity=28.6885245901639, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001077
- InterPro:   IPR005031
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00891 Methyltransf_2; PF03364 Polyketide_cyc [H]

EC number: NA

Molecular weight: Translated: 40715; Mature: 40584

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFQMNAGAVRRFAKAMRFGAWLQSITERMTPAPFRLVQIGSAYWQSKALYVAAKLDLAT
CEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEHHHHHH
VLGTATLTASALASRVGANEDALGRLMRLLAAMGIFEETAPMVFRNNKLSRCLVSDDPQS
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEECCCCHHH
VRAMILMHNSETMSRPWFEQLEAGIRSGTPPFQLSHGEDLFDYLDHHADFDQLFSEAMNS
HEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHCCCCHHHHHHHHHHH
VEALAGDGFATDLDWARFDRIIDVGGSRGTKSLAILRRHPRLTALIVDRPQVIEEAQRYW
HHHHCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHH
AEHHADGLERLQFQAADLFESLPAAGPKDVYLYSAVLHGFDDPTCVQALLRLREAIGNSG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
ARAAILEIVVPEKGADISSASFDMQMFVGSRGRERTLTEWKAIIQAGGLTLEEVVRLRSL
CCEEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCC
GSILVLRAG
CCEEEEECC
>Mature Secondary Structure 
TFQMNAGAVRRFAKAMRFGAWLQSITERMTPAPFRLVQIGSAYWQSKALYVAAKLDLAT
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEHHHHHH
VLGTATLTASALASRVGANEDALGRLMRLLAAMGIFEETAPMVFRNNKLSRCLVSDDPQS
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEECCCCHHH
VRAMILMHNSETMSRPWFEQLEAGIRSGTPPFQLSHGEDLFDYLDHHADFDQLFSEAMNS
HEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHCCCCHHHHHHHHHHH
VEALAGDGFATDLDWARFDRIIDVGGSRGTKSLAILRRHPRLTALIVDRPQVIEEAQRYW
HHHHCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHH
AEHHADGLERLQFQAADLFESLPAAGPKDVYLYSAVLHGFDDPTCVQALLRLREAIGNSG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
ARAAILEIVVPEKGADISSASFDMQMFVGSRGRERTLTEWKAIIQAGGLTLEEVVRLRSL
CCEEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCC
GSILVLRAG
CCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1548230; 2684656 [H]