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The map label for this gene is lcfA [H]

Identifier: 94313945

GI number: 94313945

Start: 1708067

End: 1709962

Strand: Direct

Name: lcfA [H]

Synonym: Rmet_5023

Alternate gene names: 94313945

Gene position: 1708067-1709962 (Clockwise)

Preceding gene: 94313944

Following gene: 94313946

Centisome position: 66.2

GC content: 68.09

Gene sequence:

>1896_bases
ATGACCGCAACGTCCGCTGCCCGCAGCGACACCGCGCCCAAGTTCCGGCCAACGGTATTCCCGCCGCGCACCACGCTCGT
CGAGCAGCGTGCCGACGGCACCATCGTCCTGCGCTGCGCCAACCCGCCGGCCGACCCCGCCCAGCTCGGACTCGGCGAGT
TTGCCGCCCACTGGGCGGTTCAACGCGGCGATACCTGCGCGTTCGCCGAACGCGAATCGACCAGTCCGGACGCCGCATGG
CGGATGATCACCTGGGCCGAGATGTGGCAACAAATCCAGTCCGTCGGCGCCGCCCTGCTGGAGATGGGCCTGGGCGCTGA
CTGTCCGCTGATGATCCTCTCTGGCAACTCGATCGAACACGCCATACTAGTGCTGGCAGCCGAGCACGTCGGCGTGCCCA
CGGCGCCGGTCTCGCCCGCTTATTCGCTGGTGAGCAAGGATTTCGCGCGACTCCGCGACGTAGTGAGCCTGGTGCCGCCG
GGTGCGATCTTCGTGCAGGATGCCGCCGCCTTCGCCCGCGCGCTCGACGTCATCGGCAAGCCCGATGTGCCCGTCATCGC
GGTGCGCGGCGCGCGCAAAGGCCACCACGACTGGGCCTCGCTGGTCGCCACCACCGTGACGCCGGCACGCCGCGCGGCAG
TGCAGGCCGCGCACGATGCCATCCACGAAGACGACCTGGGCCGCGTGCTGTTCACATCCGGCTCCACCGGTGTGCCCAAG
GGCGTGGCACTGTCGCACGGCAACCTCCGCGCCGTGGCTGCTTACTACCTGGAGAGCTTTCGGCCGCTGCTGGAACGCCC
ATCCCGCTACCTCGACTGGCTGCCATGGCACCACGGCCTGGGTGGCGTGCTGAACATGACGCGCGTGGCCACGTTGGGCG
GCAGCCACTACATCGACGATGGACGCCCGCTGCCGGGTCAGTTCGATCGCACCGTACGAAATATGCGCGATGTCGCTGCA
ACCGTGTACACCGCCGTGCCCGCCGCCTGGATGATGCTGGTGACCGAGGCCGAGCGCAATCCGGAAGTCGCACGCAACCT
GTTCTCCGAGGCGGTGAACTTCTCCTACGGTGGCGCCAGCCTGCCGCGCGATGTCTGGGACCGCATCCACGCGCTGTCCG
AACGCACCATTGGCGAGCGCGTGGCGTTCTGCTCGGGCCTCGCTTGCACCGAGTCGAGCGGCGTGGGCCTCTACTGCACG
TGGCCAAGCGAGACCACTGGGAACATCGGCGTGCCCGCGCCCGGCGTCTCCGCGAAGCTCATCCCGCTCGAAGGCGGTGA
TGGCCGCTACGAGATCCGCATCAAGGGCCCCAGCGTATTCAAGGGCTACGTACAGCGCCCCGACCTCACTGCCGCCGCAT
TCGATGAAGAGGGCTTCTTCAAGATGGGAGACGCGGTGCGTCTGCAGGACCCCTCCGATCCTGCACGCGGCCTGTCGTTC
GCCGGCCGCGTGGCCGAGGACTTCAAACTCACCAACGGCACCTGGGTCCGTACCGGCGCGGTGCGCCTTACGCTGGTCGA
ACAATGCGCGCCGCTGCTCAACGATGCAGTGATCTGCGGCCACGACCACGACTACCTGGCGGCGCTGGCCTGGCCCAACG
TCGCAGCCTGCCGGAGTCTTGCGCCCGAACTGTCGGAGCTCGACGCCGAGGCGCTGGTCCGGCACCCCATCGTCGTCGAA
GCCATTCGCACTCGGCTACACGCGCCGGCGAGCGGCAAGGGCGCCAGTCTGTCCGTGCGTCGCGTGATGCTGATGGCGGA
ACCGCCGTCGATGGACGCGAACGAAATCGCCGACAAGGGCTACGTAAATCAGGCCGCCACGCGGGCACGCCGCGCCCATC
TGGTCGAAGCCCTGTTTCATCCCGAACCCGAAAGCCACATTGCCTGCGCGCGATAA

Upstream 100 bases:

>100_bases
CGTAACTTAGCGTCATTCGATCGCACGTCCGATCCCGCGGGATCGGCAGGCGACAAACGCCCTGCGGCACCATGTGCCGC
TGACCGAGGAGACGCGTTCC

Downstream 100 bases:

>100_bases
GCCCGCCCGCACGACACCAACTCAAGAGGAGACTGTCATGCAAGTTCAACGTAACGTTTACCGCGAAGATCACGAGATGT
TCCGCGAAACCGCCCGCCGC

Product: feruloyl-CoA synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MTATSAARSDTAPKFRPTVFPPRTTLVEQRADGTIVLRCANPPADPAQLGLGEFAAHWAVQRGDTCAFAERESTSPDAAW
RMITWAEMWQQIQSVGAALLEMGLGADCPLMILSGNSIEHAILVLAAEHVGVPTAPVSPAYSLVSKDFARLRDVVSLVPP
GAIFVQDAAAFARALDVIGKPDVPVIAVRGARKGHHDWASLVATTVTPARRAAVQAAHDAIHEDDLGRVLFTSGSTGVPK
GVALSHGNLRAVAAYYLESFRPLLERPSRYLDWLPWHHGLGGVLNMTRVATLGGSHYIDDGRPLPGQFDRTVRNMRDVAA
TVYTAVPAAWMMLVTEAERNPEVARNLFSEAVNFSYGGASLPRDVWDRIHALSERTIGERVAFCSGLACTESSGVGLYCT
WPSETTGNIGVPAPGVSAKLIPLEGGDGRYEIRIKGPSVFKGYVQRPDLTAAAFDEEGFFKMGDAVRLQDPSDPARGLSF
AGRVAEDFKLTNGTWVRTGAVRLTLVEQCAPLLNDAVICGHDHDYLAALAWPNVAACRSLAPELSELDAEALVRHPIVVE
AIRTRLHAPASGKGASLSVRRVMLMAEPPSMDANEIADKGYVNQAATRARRAHLVEALFHPEPESHIACAR

Sequences:

>Translated_631_residues
MTATSAARSDTAPKFRPTVFPPRTTLVEQRADGTIVLRCANPPADPAQLGLGEFAAHWAVQRGDTCAFAERESTSPDAAW
RMITWAEMWQQIQSVGAALLEMGLGADCPLMILSGNSIEHAILVLAAEHVGVPTAPVSPAYSLVSKDFARLRDVVSLVPP
GAIFVQDAAAFARALDVIGKPDVPVIAVRGARKGHHDWASLVATTVTPARRAAVQAAHDAIHEDDLGRVLFTSGSTGVPK
GVALSHGNLRAVAAYYLESFRPLLERPSRYLDWLPWHHGLGGVLNMTRVATLGGSHYIDDGRPLPGQFDRTVRNMRDVAA
TVYTAVPAAWMMLVTEAERNPEVARNLFSEAVNFSYGGASLPRDVWDRIHALSERTIGERVAFCSGLACTESSGVGLYCT
WPSETTGNIGVPAPGVSAKLIPLEGGDGRYEIRIKGPSVFKGYVQRPDLTAAAFDEEGFFKMGDAVRLQDPSDPARGLSF
AGRVAEDFKLTNGTWVRTGAVRLTLVEQCAPLLNDAVICGHDHDYLAALAWPNVAACRSLAPELSELDAEALVRHPIVVE
AIRTRLHAPASGKGASLSVRRVMLMAEPPSMDANEIADKGYVNQAATRARRAHLVEALFHPEPESHIACAR
>Mature_630_residues
TATSAARSDTAPKFRPTVFPPRTTLVEQRADGTIVLRCANPPADPAQLGLGEFAAHWAVQRGDTCAFAERESTSPDAAWR
MITWAEMWQQIQSVGAALLEMGLGADCPLMILSGNSIEHAILVLAAEHVGVPTAPVSPAYSLVSKDFARLRDVVSLVPPG
AIFVQDAAAFARALDVIGKPDVPVIAVRGARKGHHDWASLVATTVTPARRAAVQAAHDAIHEDDLGRVLFTSGSTGVPKG
VALSHGNLRAVAAYYLESFRPLLERPSRYLDWLPWHHGLGGVLNMTRVATLGGSHYIDDGRPLPGQFDRTVRNMRDVAAT
VYTAVPAAWMMLVTEAERNPEVARNLFSEAVNFSYGGASLPRDVWDRIHALSERTIGERVAFCSGLACTESSGVGLYCTW
PSETTGNIGVPAPGVSAKLIPLEGGDGRYEIRIKGPSVFKGYVQRPDLTAAAFDEEGFFKMGDAVRLQDPSDPARGLSFA
GRVAEDFKLTNGTWVRTGAVRLTLVEQCAPLLNDAVICGHDHDYLAALAWPNVAACRSLAPELSELDAEALVRHPIVVEA
IRTRLHAPASGKGASLSVRRVMLMAEPPSMDANEIADKGYVNQAATRARRAHLVEALFHPEPESHIACAR

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42794756, Length=418, Percent_Identity=24.8803827751196, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI42794760, Length=418, Percent_Identity=24.8803827751196, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI42794758, Length=418, Percent_Identity=24.8803827751196, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788107, Length=270, Percent_Identity=28.5185185185185, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556552, Length=316, Percent_Identity=27.2151898734177, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25147511, Length=424, Percent_Identity=25.4716981132075, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 67819; Mature: 67688

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATSAARSDTAPKFRPTVFPPRTTLVEQRADGTIVLRCANPPADPAQLGLGEFAAHWAV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHCHHHHHHHHHH
QRGDTCAFAERESTSPDAAWRMITWAEMWQQIQSVGAALLEMGLGADCPLMILSGNSIEH
HCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCE
AILVLAAEHVGVPTAPVSPAYSLVSKDFARLRDVVSLVPPGAIFVQDAAAFARALDVIGK
EEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCC
PDVPVIAVRGARKGHHDWASLVATTVTPARRAAVQAAHDAIHEDDLGRVLFTSGSTGVPK
CCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
GVALSHGNLRAVAAYYLESFRPLLERPSRYLDWLPWHHGLGGVLNMTRVATLGGSHYIDD
CEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCEECCC
GRPLPGQFDRTVRNMRDVAATVYTAVPAAWMMLVTEAERNPEVARNLFSEAVNFSYGGAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
LPRDVWDRIHALSERTIGERVAFCSGLACTESSGVGLYCTWPSETTGNIGVPAPGVSAKL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCCCCCCCCCCEEE
IPLEGGDGRYEIRIKGPSVFKGYVQRPDLTAAAFDEEGFFKMGDAVRLQDPSDPARGLSF
EEEECCCCEEEEEECCCHHHHHHHCCCCCEEEEECCCCCEECCCEEEECCCCCHHHHHHH
AGRVAEDFKLTNGTWVRTGAVRLTLVEQCAPLLNDAVICGHDHDYLAALAWPNVAACRSL
HHHHHHHEEECCCCEEEECCEEEHHHHHHHHHHCCCEEECCCCCEEEEECCCCHHHHHHH
APELSELDAEALVRHPIVVEAIRTRLHAPASGKGASLSVRRVMLMAEPPSMDANEIADKG
CHHHHHCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHCC
YVNQAATRARRAHLVEALFHPEPESHIACAR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TATSAARSDTAPKFRPTVFPPRTTLVEQRADGTIVLRCANPPADPAQLGLGEFAAHWAV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHCHHHHHHHHHH
QRGDTCAFAERESTSPDAAWRMITWAEMWQQIQSVGAALLEMGLGADCPLMILSGNSIEH
HCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCE
AILVLAAEHVGVPTAPVSPAYSLVSKDFARLRDVVSLVPPGAIFVQDAAAFARALDVIGK
EEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCC
PDVPVIAVRGARKGHHDWASLVATTVTPARRAAVQAAHDAIHEDDLGRVLFTSGSTGVPK
CCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
GVALSHGNLRAVAAYYLESFRPLLERPSRYLDWLPWHHGLGGVLNMTRVATLGGSHYIDD
CEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCEECCC
GRPLPGQFDRTVRNMRDVAATVYTAVPAAWMMLVTEAERNPEVARNLFSEAVNFSYGGAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
LPRDVWDRIHALSERTIGERVAFCSGLACTESSGVGLYCTWPSETTGNIGVPAPGVSAKL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCCCCCCCCCCEEE
IPLEGGDGRYEIRIKGPSVFKGYVQRPDLTAAAFDEEGFFKMGDAVRLQDPSDPARGLSF
EEEECCCCEEEEEECCCHHHHHHHCCCCCEEEEECCCCCEECCCEEEECCCCCHHHHHHH
AGRVAEDFKLTNGTWVRTGAVRLTLVEQCAPLLNDAVICGHDHDYLAALAWPNVAACRSL
HHHHHHHEEECCCCEEEECCEEEHHHHHHHHHHCCCEEECCCCCEEEEECCCCHHHHHHH
APELSELDAEALVRHPIVVEAIRTRLHAPASGKGASLSVRRVMLMAEPPSMDANEIADKG
CHHHHHCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHCC
YVNQAATRARRAHLVEALFHPEPESHIACAR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]