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The map label for this gene is nosX [H]

Identifier: 94313841

GI number: 94313841

Start: 1582577

End: 1583599

Strand: Direct

Name: nosX [H]

Synonym: Rmet_4919

Alternate gene names: 94313841

Gene position: 1582577-1583599 (Clockwise)

Preceding gene: 94313840

Following gene: 94313842

Centisome position: 61.34

GC content: 66.67

Gene sequence:

>1023_bases
ATGCACGCGCTCTCTCCTTCCGGCCTTGCCCGGCGGCGCTACCTGACGGCGGCACCGCTGCTTGCCCTCGGCATGTTCGT
CCTGCCGGCATTGGCCGATTCATCGGTCCGGCACGCCTCACGCCGGCTGCTCGGCACGCGGGTGGACATCGTGACCGACG
GCGTGCCAGCGGATGCGGCCGCGCAGGCCATCGACGCGGCGTTCGCCGAGATGGGCAGGCTCGAACGGATCATGAGCCGC
TATCGTCCGGACAGTCAGGTCAGTGCCCTGGCACGTGCTGCCGGCAAGCACCCGGTACCCATCGCGCCCGAGTTGATGTC
GGCGCTCAGGTTGGCTAGCGAGGTCTCCGCGCGCAGCCAGGGCGCCTTCGACATTACGGTCGGCGCGTTTGAAGGCTGGA
ACTTCGATCCGGGCCATGAGCAAGTGCCCGGGCAAGCCGAACTGACGCAAGAGCGACCACTAGTGAACTACCGTGATGTC
GTTCTGGACTCAACGCGCGGTAGCGCCTATCTACGGCGTCCCGGCATGAAGCTGGATCTGGGCGGCATCGCCAAGCTCCC
GATCCTGCAAGCCGGCATGGACGTGCTGCGGCAGCAAGGCATCTCGAATGCGATGCTCAATGGCGGAGGCGACGTGCTGG
TCAGCGGCAGGCTTCAGGGCCGCGACTGGCGTATCGGCCTGCGCGACCCGCTTGCGCCGGAGCGATTGCTGGGAGTAGTG
GCGATCACCGATGGCGTCGTGGCGTCGTCGGGCGACTATGAGCGCTGCTTTGCCCGTGATGGCCAGCGCTATCACCACAT
CCTTGACCCTCGAACCGGCCTGCCAACCCGGGGGCCGCACGGGGTCACGCTGGTCGCGCGCAAAACAAGTGACGTCAACG
GCCTCGGGGCCGCTATCATGGTGGCTGGTACAGCGGCAGGACGGAGCCTGCTGGGGCCCATGGCTAACGTGGATGCTCTG
ATTGTCGGGCCCGGGTCTCATCCATGGATGTCCGAAGGCATGGCGACCCGTATGCGGTCGTGA

Upstream 100 bases:

>100_bases
AGCAAGGGATCGATGCCCGCGCGCGGTGGCTCGACAACGCTGCAGGATGACGCCGTCAAGGCAGCGGTGGACTACATGAC
GGCACAATCCCGCTGACGCC

Downstream 100 bases:

>100_bases
AACTATGATTTAGCGCATGGCTTCACGTGGGGAGCCGCGCTACCCTCCCACTCCTGACGCAGAGACACTAGCAGAATGAA
GCAGAGCGGCCCCGGTTCGG

Product: membrane-associated ApbE-like lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MHALSPSGLARRRYLTAAPLLALGMFVLPALADSSVRHASRRLLGTRVDIVTDGVPADAAAQAIDAAFAEMGRLERIMSR
YRPDSQVSALARAAGKHPVPIAPELMSALRLASEVSARSQGAFDITVGAFEGWNFDPGHEQVPGQAELTQERPLVNYRDV
VLDSTRGSAYLRRPGMKLDLGGIAKLPILQAGMDVLRQQGISNAMLNGGGDVLVSGRLQGRDWRIGLRDPLAPERLLGVV
AITDGVVASSGDYERCFARDGQRYHHILDPRTGLPTRGPHGVTLVARKTSDVNGLGAAIMVAGTAAGRSLLGPMANVDAL
IVGPGSHPWMSEGMATRMRS

Sequences:

>Translated_340_residues
MHALSPSGLARRRYLTAAPLLALGMFVLPALADSSVRHASRRLLGTRVDIVTDGVPADAAAQAIDAAFAEMGRLERIMSR
YRPDSQVSALARAAGKHPVPIAPELMSALRLASEVSARSQGAFDITVGAFEGWNFDPGHEQVPGQAELTQERPLVNYRDV
VLDSTRGSAYLRRPGMKLDLGGIAKLPILQAGMDVLRQQGISNAMLNGGGDVLVSGRLQGRDWRIGLRDPLAPERLLGVV
AITDGVVASSGDYERCFARDGQRYHHILDPRTGLPTRGPHGVTLVARKTSDVNGLGAAIMVAGTAAGRSLLGPMANVDAL
IVGPGSHPWMSEGMATRMRS
>Mature_340_residues
MHALSPSGLARRRYLTAAPLLALGMFVLPALADSSVRHASRRLLGTRVDIVTDGVPADAAAQAIDAAFAEMGRLERIMSR
YRPDSQVSALARAAGKHPVPIAPELMSALRLASEVSARSQGAFDITVGAFEGWNFDPGHEQVPGQAELTQERPLVNYRDV
VLDSTRGSAYLRRPGMKLDLGGIAKLPILQAGMDVLRQQGISNAMLNGGGDVLVSGRLQGRDWRIGLRDPLAPERLLGVV
AITDGVVASSGDYERCFARDGQRYHHILDPRTGLPTRGPHGVTLVARKTSDVNGLGAAIMVAGTAAGRSLLGPMANVDAL
IVGPGSHPWMSEGMATRMRS

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=316, Percent_Identity=23.4177215189873, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 36100; Mature: 36100

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHALSPSGLARRRYLTAAPLLALGMFVLPALADSSVRHASRRLLGTRVDIVTDGVPADAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEECCCCCHHH
AQAIDAAFAEMGRLERIMSRYRPDSQVSALARAAGKHPVPIAPELMSALRLASEVSARSQ
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
GAFDITVGAFEGWNFDPGHEQVPGQAELTQERPLVNYRDVVLDSTRGSAYLRRPGMKLDL
CCEEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCHHHHEEECCCCCEEEECCCCEECC
GGIAKLPILQAGMDVLRQQGISNAMLNGGGDVLVSGRLQGRDWRIGLRDPLAPERLLGVV
CCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCEEEECEECCCCEEECCCCCCCHHHHHEEE
AITDGVVASSGDYERCFARDGQRYHHILDPRTGLPTRGPHGVTLVARKTSDVNGLGAAIM
EEECCEEECCCCHHHHHHCCCCHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEE
VAGTAAGRSLLGPMANVDALIVGPGSHPWMSEGMATRMRS
EECCHHCHHHHCCCCCCCEEEECCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MHALSPSGLARRRYLTAAPLLALGMFVLPALADSSVRHASRRLLGTRVDIVTDGVPADAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEECCCCCHHH
AQAIDAAFAEMGRLERIMSRYRPDSQVSALARAAGKHPVPIAPELMSALRLASEVSARSQ
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
GAFDITVGAFEGWNFDPGHEQVPGQAELTQERPLVNYRDVVLDSTRGSAYLRRPGMKLDL
CCEEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCHHHHEEECCCCCEEEECCCCEECC
GGIAKLPILQAGMDVLRQQGISNAMLNGGGDVLVSGRLQGRDWRIGLRDPLAPERLLGVV
CCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCEEEECEECCCCEEECCCCCCCHHHHHEEE
AITDGVVASSGDYERCFARDGQRYHHILDPRTGLPTRGPHGVTLVARKTSDVNGLGAAIM
EEECCEEECCCCHHHHHHCCCCHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEE
VAGTAAGRSLLGPMANVDALIVGPGSHPWMSEGMATRMRS
EECCHHCHHHHCCCCCCCEEEECCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]