| Definition | Cupriavidus metallidurans CH34 megaplasmid, complete sequence. |
|---|---|
| Accession | NC_007974 |
| Length | 2,580,084 |
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The map label for this gene is mrcA [H]
Identifier: 94313757
GI number: 94313757
Start: 1489588
End: 1492068
Strand: Direct
Name: mrcA [H]
Synonym: Rmet_4835
Alternate gene names: 94313757
Gene position: 1489588-1492068 (Clockwise)
Preceding gene: 94313755
Following gene: 94313761
Centisome position: 57.73
GC content: 66.91
Gene sequence:
>2481_bases ATGCGCCTGGATTCCATCAAGACCTTCATCGCCTCACGCCTGTCGCTGATCCGTCAGCATTTGCGCCGTCCCACGCGGCG CGAGGTCGTGCTGGCGGTGGCCGCCATCCCGGCGCTGGTGCTGCTCTATATCCTGTTGCTGATCCCGTTCACGCCGGGCA TCAGCGACATCCGCAAGGCCAAGTCCGAACAGCCCGCGCAGGTACTGTCGGCCGACGGCAAGGAAATCGCGGTCTACCGC TGGGCCAACCGCGACTGGGTCAAGCTTTCCGAGATCTCGCCCAATGTGGTCGACGCGCTGATCGCCACCGAGGACCACCG CTTCTACCAGCACCACGGGCTCGACTGGCGGCGCACCGCCTCGGCCGCGCTGCATACGTTCTCGGGCGACCGCCAGGGCG GTTCCACGCTCACGCAGCAGCTGGCGCGCAACCGCTACCCGGACGAGATCGGCCGCGCGCCCACTTTGACGCGCAAGCTC AAGGAAGCGATCACGGCACTGAAGATCGAAGCGCTTTACTCGAAGGACGAGATCCTCGAGACCTACCTCAACACGGTGCC GTTCCTGTACAACGCGTTCGGCATCGAGATGGCCGCGCGCACGTACTTCGACAAGTCCGCCGACAAGCTCGACGTGCTGG AAGCCGCGACGCTGATCGGGATGCTCAAGGGCAACAGCTACTACAACCCGGTGCTGAACCCCGAGCGCGCGCTGGATCGC CGCAACACGGTGCTGGCCCAGATGGTGAAACGCGGCAAGCTCGACCCGGCCCGCTTCGAGACGCTGAAGAAGCGGCCACT GCGCATCGACTTCGAGCGCCAGATCGAGGCACCCGGCCCCGCACCCCACTTCGCCCAGCAACTGCGCAAATGGCTGATTA CCTGGGCTGACCGCAACGACTACAACCTCTATACCGACGGCCTGGTCATCCATACGACGATCGATTCGCGTCTGCAGACC ATGGCCACCCAGGCACTGACGCGCCAGGGCAACCAACTGCAATCGATCGCCAACGCTGCCTGGGCACCGCGCGCGGGATG GTCCGAGAGCCGGGGTCTGGTGCAGGCATTCGTGCGCGAAACGCCGGAGTTCCGCGCCGCGGTGGCTGGCGGCCAGAGCG AGGAAGACGCGCTGAAGCGCCTGATGGCCGATCGCCCCTTTATGCAGGCGCTGCGCGAGCAGAAGACGCGCGTGCAGGCG GGCTTCATGGCGATGGACCCGACAACCGGCGAAATCAAGGCCTGGGTTGGCAGCCGCGACTTCACAGTCGACGCGTTCGA CCACGTAGCCCAGGCGCGTCGCCAGCCCGGTTCCACGTTCAAGCCTTTTGTCTATGGCGCCGCGTTCGCGCGCGGGCAGA GTCCCGACGAGACCTTTATCGACCAGCCGGTCGAGATCAAGCTGGCCGGTGGCGAAATCTGGCGCCCCACCGATGAATCG CCGCCCACGGGCCGCGCGCTGAGCCTGCGTGACGGTCTGGTCTACTCGAAGAACACGATCACCGCCCAACTGGTGCAGAC GATTGGCGCGGACCGCGTGGCGCAACTGGCCCGTGCGATGGGCGTGCGCGACAGCAAGCTCGAAGCCGTGCCATCGCTGG CGCTGGGCACGAGTCCGGTGACGCTCAAGGAAATGGTCAGCGCCTACGGCACCATCGCCAATGGCGGGCAATACCTCGCG CCGACCATGGTCACGCGGATCGAGGACCAGCAAGGCAATGTGCTGGCGCAGTTCCGCGATCCGTCACCGCAACGCGCGTT GTCGACCTCGGCCAACGAGACGCTCGTCGACGTGATGCGCGACGTGATCGACCGCGGCACCGGCACGGCGATCCGCACCC GCTTCGGCATCCGTGCCGACGTGGCGGGCAAGACCGGCACCACCCAGGACAACGCCGACGGCTGGTTTATCCTGATGCAT CCCCAGCTCGTGGCCGGCGCATGGGTCGGCTTCAACGACAGCCGCGTGACGCTACGTAGCGACTACTGGGGCCAGGGCGC ACATAGCGCGCTGCCGATCGTCGGTGACTTCTATCAACGGGCCCTGCGCGCGAGGATCATCGACCCGCGCGTGCGCTTCG CGGAATCCAACGAGAAGACCTGGTACTCCGCGATTACCGATCAGATCAAGGGCTGGTACCAGAAGCTGTTCGGCGCCGGC AAGAAGGAAGACGCCGCGCCCGTTCCACGTCCGGCGCCGCGCCGCCCGGCACTGCCGGAGACCGATAACGACGCATTGCC ACCGGCCAGCGCATCGGCGGCAATCGCCGACGGCGGCATGGCACTCGACCCGGGTGAAGTGGTGGAAGCCACGCCACCGT CGTCTTCCGCAAGCGCCCCGGCAGCCCCGGCCGCTCCAACCGCGCCTGCCATCCCGGCATCAGCGCCGTCCGTGCCGGTG GTCTCGCCCGTACCGCCAGTGGCACCTTCGGTGGCCGGCGAGCCGGCTCAGTCGGATGCGGCGCCGGCTACAGCGCACTG A
Upstream 100 bases:
>100_bases GGGTTTTCCCTGCCCACAATTTGGTAGCATTCACACTTTTCCGAGAACGGCATTTGACGCCCCGATGACAGCGCGCAACG GGACCGAGGTGCCTGTGTCA
Downstream 100 bases:
>100_bases CACAGCCACACAGACGCTGGCAGTGATTCAGGGCGCCGCGCGCAACGCGCGCAGCGCCTCCTCCGACAATGTCAACGCCG GTACCTCGGCGGGACGTGCC
Product: peptidoglycan glycosyl transferase
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 826; Mature: 826
Protein sequence:
>826_residues MRLDSIKTFIASRLSLIRQHLRRPTRREVVLAVAAIPALVLLYILLLIPFTPGISDIRKAKSEQPAQVLSADGKEIAVYR WANRDWVKLSEISPNVVDALIATEDHRFYQHHGLDWRRTASAALHTFSGDRQGGSTLTQQLARNRYPDEIGRAPTLTRKL KEAITALKIEALYSKDEILETYLNTVPFLYNAFGIEMAARTYFDKSADKLDVLEAATLIGMLKGNSYYNPVLNPERALDR RNTVLAQMVKRGKLDPARFETLKKRPLRIDFERQIEAPGPAPHFAQQLRKWLITWADRNDYNLYTDGLVIHTTIDSRLQT MATQALTRQGNQLQSIANAAWAPRAGWSESRGLVQAFVRETPEFRAAVAGGQSEEDALKRLMADRPFMQALREQKTRVQA GFMAMDPTTGEIKAWVGSRDFTVDAFDHVAQARRQPGSTFKPFVYGAAFARGQSPDETFIDQPVEIKLAGGEIWRPTDES PPTGRALSLRDGLVYSKNTITAQLVQTIGADRVAQLARAMGVRDSKLEAVPSLALGTSPVTLKEMVSAYGTIANGGQYLA PTMVTRIEDQQGNVLAQFRDPSPQRALSTSANETLVDVMRDVIDRGTGTAIRTRFGIRADVAGKTGTTQDNADGWFILMH PQLVAGAWVGFNDSRVTLRSDYWGQGAHSALPIVGDFYQRALRARIIDPRVRFAESNEKTWYSAITDQIKGWYQKLFGAG KKEDAAPVPRPAPRRPALPETDNDALPPASASAAIADGGMALDPGEVVEATPPSSSASAPAAPAAPTAPAIPASAPSVPV VSPVPPVAPSVAGEPAQSDAAPATAH
Sequences:
>Translated_826_residues MRLDSIKTFIASRLSLIRQHLRRPTRREVVLAVAAIPALVLLYILLLIPFTPGISDIRKAKSEQPAQVLSADGKEIAVYR WANRDWVKLSEISPNVVDALIATEDHRFYQHHGLDWRRTASAALHTFSGDRQGGSTLTQQLARNRYPDEIGRAPTLTRKL KEAITALKIEALYSKDEILETYLNTVPFLYNAFGIEMAARTYFDKSADKLDVLEAATLIGMLKGNSYYNPVLNPERALDR RNTVLAQMVKRGKLDPARFETLKKRPLRIDFERQIEAPGPAPHFAQQLRKWLITWADRNDYNLYTDGLVIHTTIDSRLQT MATQALTRQGNQLQSIANAAWAPRAGWSESRGLVQAFVRETPEFRAAVAGGQSEEDALKRLMADRPFMQALREQKTRVQA GFMAMDPTTGEIKAWVGSRDFTVDAFDHVAQARRQPGSTFKPFVYGAAFARGQSPDETFIDQPVEIKLAGGEIWRPTDES PPTGRALSLRDGLVYSKNTITAQLVQTIGADRVAQLARAMGVRDSKLEAVPSLALGTSPVTLKEMVSAYGTIANGGQYLA PTMVTRIEDQQGNVLAQFRDPSPQRALSTSANETLVDVMRDVIDRGTGTAIRTRFGIRADVAGKTGTTQDNADGWFILMH PQLVAGAWVGFNDSRVTLRSDYWGQGAHSALPIVGDFYQRALRARIIDPRVRFAESNEKTWYSAITDQIKGWYQKLFGAG KKEDAAPVPRPAPRRPALPETDNDALPPASASAAIADGGMALDPGEVVEATPPSSSASAPAAPAAPTAPAIPASAPSVPV VSPVPPVAPSVAGEPAQSDAAPATAH >Mature_826_residues MRLDSIKTFIASRLSLIRQHLRRPTRREVVLAVAAIPALVLLYILLLIPFTPGISDIRKAKSEQPAQVLSADGKEIAVYR WANRDWVKLSEISPNVVDALIATEDHRFYQHHGLDWRRTASAALHTFSGDRQGGSTLTQQLARNRYPDEIGRAPTLTRKL KEAITALKIEALYSKDEILETYLNTVPFLYNAFGIEMAARTYFDKSADKLDVLEAATLIGMLKGNSYYNPVLNPERALDR RNTVLAQMVKRGKLDPARFETLKKRPLRIDFERQIEAPGPAPHFAQQLRKWLITWADRNDYNLYTDGLVIHTTIDSRLQT MATQALTRQGNQLQSIANAAWAPRAGWSESRGLVQAFVRETPEFRAAVAGGQSEEDALKRLMADRPFMQALREQKTRVQA GFMAMDPTTGEIKAWVGSRDFTVDAFDHVAQARRQPGSTFKPFVYGAAFARGQSPDETFIDQPVEIKLAGGEIWRPTDES PPTGRALSLRDGLVYSKNTITAQLVQTIGADRVAQLARAMGVRDSKLEAVPSLALGTSPVTLKEMVSAYGTIANGGQYLA PTMVTRIEDQQGNVLAQFRDPSPQRALSTSANETLVDVMRDVIDRGTGTAIRTRFGIRADVAGKTGTTQDNADGWFILMH PQLVAGAWVGFNDSRVTLRSDYWGQGAHSALPIVGDFYQRALRARIIDPRVRFAESNEKTWYSAITDQIKGWYQKLFGAG KKEDAAPVPRPAPRRPALPETDNDALPPASASAAIADGGMALDPGEVVEATPPSSSASAPAAPAAPTAPAIPASAPSVPV VSPVPPVAPSVAGEPAQSDAAPATAH
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG0744
COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=608, Percent_Identity=31.0855263157895, Blast_Score=259, Evalue=4e-70, Organism=Escherichia coli, GI1786343, Length=598, Percent_Identity=29.5986622073579, Blast_Score=186, Evalue=4e-48, Organism=Escherichia coli, GI1788867, Length=233, Percent_Identity=34.3347639484979, Blast_Score=91, Evalue=2e-19, Organism=Escherichia coli, GI1789601, Length=174, Percent_Identity=37.9310344827586, Blast_Score=88, Evalue=2e-18,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.- [C]
Molecular weight: Translated: 90318; Mature: 90318
Theoretical pI: Translated: 9.70; Mature: 9.70
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 1.8 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 1.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRLDSIKTFIASRLSLIRQHLRRPTRREVVLAVAAIPALVLLYILLLIPFTPGISDIRKA CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH KSEQPAQVLSADGKEIAVYRWANRDWVKLSEISPNVVDALIATEDHRFYQHHGLDWRRTA CCCCCHHHHHCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHCCCHHHHHHCCCCHHHHH SAALHTFSGDRQGGSTLTQQLARNRYPDEIGRAPTLTRKLKEAITALKIEALYSKDEILE HHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH TYLNTVPFLYNAFGIEMAARTYFDKSADKLDVLEAATLIGMLKGNSYYNPVLNPERALDR HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH RNTVLAQMVKRGKLDPARFETLKKRPLRIDFERQIEAPGPAPHFAQQLRKWLITWADRND HHHHHHHHHHHCCCCHHHHHHHHCCCCEECHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC YNLYTDGLVIHTTIDSRLQTMATQALTRQGNQLQSIANAAWAPRAGWSESRGLVQAFVRE CEEEECCEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC TPEFRAAVAGGQSEEDALKRLMADRPFMQALREQKTRVQAGFMAMDPTTGEIKAWVGSRD CCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCCCCEEEECCCC FTVDAFDHVAQARRQPGSTFKPFVYGAAFARGQSPDETFIDQPVEIKLAGGEIWRPTDES CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHCCCCEEEEECCCCEECCCCCC PPTGRALSLRDGLVYSKNTITAQLVQTIGADRVAQLARAMGVRDSKLEAVPSLALGTSPV CCCCCEEEECCCEEECCCHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHCCHHHCCCCCC TLKEMVSAYGTIANGGQYLAPTMVTRIEDQQGNVLAQFRDPSPQRALSTSANETLVDVMR HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHH DVIDRGTGTAIRTRFGIRADVAGKTGTTQDNADGWFILMHPQLVAGAWVGFNDSRVTLRS HHHHCCCCCEEEHCCCCEEECCCCCCCCCCCCCEEEEEECCHHHHHEEECCCCCEEEEEC DYWGQGAHSALPIVGDFYQRALRARIIDPRVRFAESNEKTWYSAITDQIKGWYQKLFGAG CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC KKEDAAPVPRPAPRRPALPETDNDALPPASASAAIADGGMALDPGEVVEATPPSSSASAP CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCC AAPAAPTAPAIPASAPSVPVVSPVPPVAPSVAGEPAQSDAAPATAH CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC >Mature Secondary Structure MRLDSIKTFIASRLSLIRQHLRRPTRREVVLAVAAIPALVLLYILLLIPFTPGISDIRKA CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH KSEQPAQVLSADGKEIAVYRWANRDWVKLSEISPNVVDALIATEDHRFYQHHGLDWRRTA CCCCCHHHHHCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHCCCHHHHHHCCCCHHHHH SAALHTFSGDRQGGSTLTQQLARNRYPDEIGRAPTLTRKLKEAITALKIEALYSKDEILE HHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH TYLNTVPFLYNAFGIEMAARTYFDKSADKLDVLEAATLIGMLKGNSYYNPVLNPERALDR HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH RNTVLAQMVKRGKLDPARFETLKKRPLRIDFERQIEAPGPAPHFAQQLRKWLITWADRND HHHHHHHHHHHCCCCHHHHHHHHCCCCEECHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC YNLYTDGLVIHTTIDSRLQTMATQALTRQGNQLQSIANAAWAPRAGWSESRGLVQAFVRE CEEEECCEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC TPEFRAAVAGGQSEEDALKRLMADRPFMQALREQKTRVQAGFMAMDPTTGEIKAWVGSRD CCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCCCCEEEECCCC FTVDAFDHVAQARRQPGSTFKPFVYGAAFARGQSPDETFIDQPVEIKLAGGEIWRPTDES CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHCCCCEEEEECCCCEECCCCCC PPTGRALSLRDGLVYSKNTITAQLVQTIGADRVAQLARAMGVRDSKLEAVPSLALGTSPV CCCCCEEEECCCEEECCCHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHCCHHHCCCCCC TLKEMVSAYGTIANGGQYLAPTMVTRIEDQQGNVLAQFRDPSPQRALSTSANETLVDVMR HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHH DVIDRGTGTAIRTRFGIRADVAGKTGTTQDNADGWFILMHPQLVAGAWVGFNDSRVTLRS HHHHCCCCCEEEHCCCCEEECCCCCCCCCCCCCEEEEEECCHHHHHEEECCCCCEEEEEC DYWGQGAHSALPIVGDFYQRALRARIIDPRVRFAESNEKTWYSAITDQIKGWYQKLFGAG CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC KKEDAAPVPRPAPRRPALPETDNDALPPASASAAIADGGMALDPGEVVEATPPSSSASAP CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCC AAPAAPTAPAIPASAPSVPVVSPVPPVAPSVAGEPAQSDAAPATAH CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 10984043; 7901733 [H]