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The map label for this gene is garD [H]

Identifier: 94313656

GI number: 94313656

Start: 1377196

End: 1378788

Strand: Reverse

Name: garD [H]

Synonym: Rmet_4734

Alternate gene names: 94313656

Gene position: 1378788-1377196 (Counterclockwise)

Preceding gene: 94313657

Following gene: 94313652

Centisome position: 53.44

GC content: 65.35

Gene sequence:

>1593_bases
ATGTCGGACTTGTCGGTACAAAACGCGCAGGGCCAGGCCGCCGAACGCGCGCCGCTCTACATCCGCATTCACGCCAATGA
CAACGTGGCCATCGTCGCCAACGACGGCGGGCTGCCAGCCGGCTCAGTATTCCCGTGCGGTCTGACGCTCGTGGAGCGCG
TGCCGCAGGGCCACAAAGTGGCGCTGGTGGATCTAAAGAAAGGTGACGCGGTTACGCGCTACAACGTGACCATCGGCTAC
GCCTTGCAGGACCTGCCGCGCGGCGCGTGGGTCAACGAGCGCGTGATTGAAATGCCAACCGCGCCGGGACTGACCGATCT
GCCGATCGGCACACGTGTGCCCGAGCCGCTGCCGCCGCTGGAAGGCTATACGTTCGAGGGTTTCCGCAATCCGGACGGAT
CGGTCGGCACGCGCAACATTCTGGCGATCACGACCACGGTGCAGTGCGTGTCCGGCGTAGTGGAGCACGCGGTGCACCGG
ATCAAGACCGAGCTGCTGCCGCGCTATCCGAACGTCGACGACGTGATCGGCCTCGAGCATACCTATGGCTGCGGCGTGGC
GATCGATGCGCCCGACGCGATCGTGCCAATCCGCACGCTGCGCAATATCGCGCTGAATCCGAACTTCGGCGGCACCGCCA
TGATGGTGAGCCTGGGGTGCGAGAAGCTGCAGCCGGAACGCCTGATGCCGCCGGGCACAATCCCGATCCAGACCGGCGCC
GGCGAGGTGCAGGTGGGCGTGGTTTGCTTGCAGGACGACAAGCACGTGGGTTTCGCGTCGATGATCGACTCGATCATGGC
GATGGCCGAGACCCATCTGCAGACCCTCAATGCCCGCAAGCGCGAGACGGTGCCCGTATCGGAACTGGTGGTGGGCGTGC
AGTGCGGCGGTAGCGATGCGTTCTCCGGCGTGACGGCCAATCCGGCTGTCGGCTTTGCCACAGACCTGCTCGTGCGCGCC
GGCGCGACCGTGATGTTCTCGGAAGTGACCGAGGTCCGCGACGGTATTGACCAGCTCACCGCCCGCGCCGCTACCCCCGA
GGTGGCCGAAGCGATGATTCGGGAAATGGACTGGTACGACCGCTATCTGGACCGTGGTCGCGTCGATCGCAGCGCCAACA
CGACGCCAGGCAACAAGAAGGGTGGCCTGTCGAATATCGTGGAAAAGGCCATGGGTTCGATCGTCAAGTCGGGCAGCGCG
CCGATCTCGGGCGTGGTGTCACCGGGCGAGAAGGTCAAGCAGAAGGGCTTGATCTACGCGGCGACCCCGGCCAGCGATTT
CATCTGTGGCACGCTGCAACTGGCTGCCGGCATGAACCTGCACATCTTCACGACGGGGCGCGGCACGCCGTATGGTCTGG
CCGAGGTGCCCGTGATCAAGGTCGCAACGCGCAGCGATCTGGCGCGCCGCTGGCACGACCTGATGGACGTGAACGCAGGC
CGCATCGCCACGGGCGAAGCCACGATCGAGGATGTGGGCTGGGAGTTGTTCCACCTGATGATCGACGTGGCCAGCGGCCG
CAAGCAGACCTGGGCCGAGCACCACAAGCTGCATAACGCGCTGGCGCTGTTCAATCCGGCGCCGGTGACCTGA

Upstream 100 bases:

>100_bases
GGTATCAATGCCCTGGTCACCGTGCTGAGCTATCTGGTGATCGTCAAGGACATTCGCCGCGTCGAGCTGAAGCACCCCCA
ATAAGGAAGGAATCCCCAAC

Downstream 100 bases:

>100_bases
GTGGTGGTTTGTTGAGGGGTGTCGCCTCTCTCCCGCAGGTGGGGGAGAGGCGGAAGATCGCCAGGCTTAGCCGCCAATGA
TCTGCTTGGAGTACGTCGCG

Product: galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSDLSVQNAQGQAAERAPLYIRIHANDNVAIVANDGGLPAGSVFPCGLTLVERVPQGHKVALVDLKKGDAVTRYNVTIGY
ALQDLPRGAWVNERVIEMPTAPGLTDLPIGTRVPEPLPPLEGYTFEGFRNPDGSVGTRNILAITTTVQCVSGVVEHAVHR
IKTELLPRYPNVDDVIGLEHTYGCGVAIDAPDAIVPIRTLRNIALNPNFGGTAMMVSLGCEKLQPERLMPPGTIPIQTGA
GEVQVGVVCLQDDKHVGFASMIDSIMAMAETHLQTLNARKRETVPVSELVVGVQCGGSDAFSGVTANPAVGFATDLLVRA
GATVMFSEVTEVRDGIDQLTARAATPEVAEAMIREMDWYDRYLDRGRVDRSANTTPGNKKGGLSNIVEKAMGSIVKSGSA
PISGVVSPGEKVKQKGLIYAATPASDFICGTLQLAAGMNLHIFTTGRGTPYGLAEVPVIKVATRSDLARRWHDLMDVNAG
RIATGEATIEDVGWELFHLMIDVASGRKQTWAEHHKLHNALALFNPAPVT

Sequences:

>Translated_530_residues
MSDLSVQNAQGQAAERAPLYIRIHANDNVAIVANDGGLPAGSVFPCGLTLVERVPQGHKVALVDLKKGDAVTRYNVTIGY
ALQDLPRGAWVNERVIEMPTAPGLTDLPIGTRVPEPLPPLEGYTFEGFRNPDGSVGTRNILAITTTVQCVSGVVEHAVHR
IKTELLPRYPNVDDVIGLEHTYGCGVAIDAPDAIVPIRTLRNIALNPNFGGTAMMVSLGCEKLQPERLMPPGTIPIQTGA
GEVQVGVVCLQDDKHVGFASMIDSIMAMAETHLQTLNARKRETVPVSELVVGVQCGGSDAFSGVTANPAVGFATDLLVRA
GATVMFSEVTEVRDGIDQLTARAATPEVAEAMIREMDWYDRYLDRGRVDRSANTTPGNKKGGLSNIVEKAMGSIVKSGSA
PISGVVSPGEKVKQKGLIYAATPASDFICGTLQLAAGMNLHIFTTGRGTPYGLAEVPVIKVATRSDLARRWHDLMDVNAG
RIATGEATIEDVGWELFHLMIDVASGRKQTWAEHHKLHNALALFNPAPVT
>Mature_529_residues
SDLSVQNAQGQAAERAPLYIRIHANDNVAIVANDGGLPAGSVFPCGLTLVERVPQGHKVALVDLKKGDAVTRYNVTIGYA
LQDLPRGAWVNERVIEMPTAPGLTDLPIGTRVPEPLPPLEGYTFEGFRNPDGSVGTRNILAITTTVQCVSGVVEHAVHRI
KTELLPRYPNVDDVIGLEHTYGCGVAIDAPDAIVPIRTLRNIALNPNFGGTAMMVSLGCEKLQPERLMPPGTIPIQTGAG
EVQVGVVCLQDDKHVGFASMIDSIMAMAETHLQTLNARKRETVPVSELVVGVQCGGSDAFSGVTANPAVGFATDLLVRAG
ATVMFSEVTEVRDGIDQLTARAATPEVAEAMIREMDWYDRYLDRGRVDRSANTTPGNKKGGLSNIVEKAMGSIVKSGSAP
ISGVVSPGEKVKQKGLIYAATPASDFICGTLQLAAGMNLHIFTTGRGTPYGLAEVPVIKVATRSDLARRWHDLMDVNAGR
IATGEATIEDVGWELFHLMIDVASGRKQTWAEHHKLHNALALFNPAPVT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=518, Percent_Identity=63.3204633204633, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1789475, Length=527, Percent_Identity=33.0170777988615, Blast_Score=215, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 56536; Mature: 56405

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLSVQNAQGQAAERAPLYIRIHANDNVAIVANDGGLPAGSVFPCGLTLVERVPQGHKV
CCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCCCEE
ALVDLKKGDAVTRYNVTIGYALQDLPRGAWVNERVIEMPTAPGLTDLPIGTRVPEPLPPL
EEEEECCCCEEEEEEEEEEEHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCC
EGYTFEGFRNPDGSVGTRNILAITTTVQCVSGVVEHAVHRIKTELLPRYPNVDDVIGLEH
CCCEECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TYGCGVAIDAPDAIVPIRTLRNIALNPNFGGTAMMVSLGCEKLQPERLMPPGTIPIQTGA
CCCCCEEECCCCCEEEHHHHHHEECCCCCCCHHEEEECCHHHCCCCCCCCCCCEEEECCC
GEVQVGVVCLQDDKHVGFASMIDSIMAMAETHLQTLNARKRETVPVSELVVGVQCGGSDA
CCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEEECCCCC
FSGVTANPAVGFATDLLVRAGATVMFSEVTEVRDGIDQLTARAATPEVAEAMIREMDWYD
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RYLDRGRVDRSANTTPGNKKGGLSNIVEKAMGSIVKSGSAPISGVVSPGEKVKQKGLIYA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCEEE
ATPASDFICGTLQLAAGMNLHIFTTGRGTPYGLAEVPVIKVATRSDLARRWHDLMDVNAG
ECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCC
RIATGEATIEDVGWELFHLMIDVASGRKQTWAEHHKLHNALALFNPAPVT
EEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
SDLSVQNAQGQAAERAPLYIRIHANDNVAIVANDGGLPAGSVFPCGLTLVERVPQGHKV
CCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCCCEE
ALVDLKKGDAVTRYNVTIGYALQDLPRGAWVNERVIEMPTAPGLTDLPIGTRVPEPLPPL
EEEEECCCCEEEEEEEEEEEHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCC
EGYTFEGFRNPDGSVGTRNILAITTTVQCVSGVVEHAVHRIKTELLPRYPNVDDVIGLEH
CCCEECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TYGCGVAIDAPDAIVPIRTLRNIALNPNFGGTAMMVSLGCEKLQPERLMPPGTIPIQTGA
CCCCCEEECCCCCEEEHHHHHHEECCCCCCCHHEEEECCHHHCCCCCCCCCCCEEEECCC
GEVQVGVVCLQDDKHVGFASMIDSIMAMAETHLQTLNARKRETVPVSELVVGVQCGGSDA
CCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEEECCCCC
FSGVTANPAVGFATDLLVRAGATVMFSEVTEVRDGIDQLTARAATPEVAEAMIREMDWYD
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RYLDRGRVDRSANTTPGNKKGGLSNIVEKAMGSIVKSGSAPISGVVSPGEKVKQKGLIYA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCEEE
ATPASDFICGTLQLAAGMNLHIFTTGRGTPYGLAEVPVIKVATRSDLARRWHDLMDVNAG
ECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCC
RIATGEATIEDVGWELFHLMIDVASGRKQTWAEHHKLHNALALFNPAPVT
EEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]