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The map label for this gene is gspF [H]

Identifier: 94313634

GI number: 94313634

Start: 1354877

End: 1356118

Strand: Reverse

Name: gspF [H]

Synonym: Rmet_4712

Alternate gene names: 94313634

Gene position: 1356118-1354877 (Counterclockwise)

Preceding gene: 94313635

Following gene: 94313633

Centisome position: 52.56

GC content: 64.01

Gene sequence:

>1242_bases
GTGAGGGGCCGGCCCGCCGGCCTGACGTGGAGAACGACGGTGACCTACATGTTGTGCGTGTTCGACAGCGGTGGCCGGGT
CAAGACCATCTATCTGGACGGCGAGTCCGCTGCCAATGTGGTGACGCAGGCGGAATCCCGAGGCCTGCGCGTGGCATCCG
TCCGTTCCACCGTCACGACCACGAACAGTCTCCGCGGATGGCGCGTGCTGCCGGGTGTGCGGTTCGACGTGGAACTGTTC
GCGCGGGAGCTTGCCGCACTGCTCGATGCCGGTGTTGGCGTGATCGACGCCTTGCGCACGCTGGGCGGCAACGATCGCAG
GGAGGCGTCGGCACGTGTCTACGGGAATCTGCTCCGGCAGCTTGAGGAAGGCCATTCGCTATCCGCAGCCATGGTGAACG
CCGCGAACGTGTTTCCCGATGTGCTGATCGCCTGCGTCAAGGCCAGCGAACAGACCGGTGGTCTCGCGGACAGCCTGCGG
CGCTACTCACGCAACAGTGCCACGCTGCAGGCGTTGCGCGCGCGCGTGATCTCTGCAGCCATCTATCCGTCGGTTCTGCT
GGTGGTGGGGATGGCGGTAGTCCTGTTCCTGCTGGCGTTCGTGGTGCCACGGTTCGCCGCGCTGCTCGAGCACAGCGGCC
GCGAACTGCCGTTGATGTCGCGCTGGTTGATGATGTGGGGCGGCGCGGTGCACCGGCACGGCTCAGCCATCGCATTGACA
TTCGCCATACTGCTTCTGGCCGGCATCGTTGCGCTACAGCGTCAGTCCATACGCGATCTGCTGGCCGACCGGCTGCTTGC
GCTGCCTGGCATCGGGCCGCATTTCCGCATCTATCGCCAATCCCAATTTTTCCGAACGTCGGCGATGCTGGTGGAAGGAG
GGATTCCCGCGGTACACGCCTTCGAGCTGTCGCAAGGGCTCGTGAGCCAATCCGATCGCGCCGCGCTGAGCACGGCACTG
CACGCGGTACGCAGCGGCAGGAAGATTTCGGAAGCCTTCCAGCAGTGTGGATTGGCCAATGCCATCACTTCTCGTTTGCT
GACCGTCGCGGAGAAGACCGGTGGCCTCGGCCCGGTGCTGGACAAGATTGCCGGATTCCAGGAGTCGCATGTCTCGCGCG
CCATCGACCTGATATCGCGGTTGATCGAGCCGGTGATGATGATTTTCATCGGCGTCGTGATCGGCGGCATTGTCGTGCTG
ATGTACATGCCGATCTTCGAGCTCGCCTCCAGCGTTCAGTAG

Upstream 100 bases:

>100_bases
TCGCCGGGGGAGCATGGCGACTACGACCTCATGTCGTTTGGCAAGGACGGACAGCCGGGAGGGACGGGAGAGAACTCAGA
CATTACTTCGTGGTGAAAGC

Downstream 100 bases:

>100_bases
ACCATCATGGACCCGATCGGCACGCATCCGGAGCAGCATGGGGGGACCACCCTCCGCGCAACGCTGCGCGAACTCGCGGC
GTCCTACGGGTCGGGCACCC

Product: Type II secretory pathway, gspf-related transmembrane protein

Products: NA

Alternate protein names: Type IV-A pilus assembly protein pilC [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MRGRPAGLTWRTTVTYMLCVFDSGGRVKTIYLDGESAANVVTQAESRGLRVASVRSTVTTTNSLRGWRVLPGVRFDVELF
ARELAALLDAGVGVIDALRTLGGNDRREASARVYGNLLRQLEEGHSLSAAMVNAANVFPDVLIACVKASEQTGGLADSLR
RYSRNSATLQALRARVISAAIYPSVLLVVGMAVVLFLLAFVVPRFAALLEHSGRELPLMSRWLMMWGGAVHRHGSAIALT
FAILLLAGIVALQRQSIRDLLADRLLALPGIGPHFRIYRQSQFFRTSAMLVEGGIPAVHAFELSQGLVSQSDRAALSTAL
HAVRSGRKISEAFQQCGLANAITSRLLTVAEKTGGLGPVLDKIAGFQESHVSRAIDLISRLIEPVMMIFIGVVIGGIVVL
MYMPIFELASSVQ

Sequences:

>Translated_413_residues
MRGRPAGLTWRTTVTYMLCVFDSGGRVKTIYLDGESAANVVTQAESRGLRVASVRSTVTTTNSLRGWRVLPGVRFDVELF
ARELAALLDAGVGVIDALRTLGGNDRREASARVYGNLLRQLEEGHSLSAAMVNAANVFPDVLIACVKASEQTGGLADSLR
RYSRNSATLQALRARVISAAIYPSVLLVVGMAVVLFLLAFVVPRFAALLEHSGRELPLMSRWLMMWGGAVHRHGSAIALT
FAILLLAGIVALQRQSIRDLLADRLLALPGIGPHFRIYRQSQFFRTSAMLVEGGIPAVHAFELSQGLVSQSDRAALSTAL
HAVRSGRKISEAFQQCGLANAITSRLLTVAEKTGGLGPVLDKIAGFQESHVSRAIDLISRLIEPVMMIFIGVVIGGIVVL
MYMPIFELASSVQ
>Mature_413_residues
MRGRPAGLTWRTTVTYMLCVFDSGGRVKTIYLDGESAANVVTQAESRGLRVASVRSTVTTTNSLRGWRVLPGVRFDVELF
ARELAALLDAGVGVIDALRTLGGNDRREASARVYGNLLRQLEEGHSLSAAMVNAANVFPDVLIACVKASEQTGGLADSLR
RYSRNSATLQALRARVISAAIYPSVLLVVGMAVVLFLLAFVVPRFAALLEHSGRELPLMSRWLMMWGGAVHRHGSAIALT
FAILLLAGIVALQRQSIRDLLADRLLALPGIGPHFRIYRQSQFFRTSAMLVEGGIPAVHAFELSQGLVSQSDRAALSTAL
HAVRSGRKISEAFQQCGLANAITSRLLTVAEKTGGLGPVLDKIAGFQESHVSRAIDLISRLIEPVMMIFIGVVIGGIVVL
MYMPIFELASSVQ

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=343, Percent_Identity=27.9883381924198, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1786295, Length=388, Percent_Identity=26.2886597938144, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44518; Mature: 44518

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGRPAGLTWRTTVTYMLCVFDSGGRVKTIYLDGESAANVVTQAESRGLRVASVRSTVTT
CCCCCCCCCHHHHHHHHHEEECCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHH
TNSLRGWRVLPGVRFDVELFARELAALLDAGVGVIDALRTLGGNDRREASARVYGNLLRQ
CCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LEEGHSLSAAMVNAANVFPDVLIACVKASEQTGGLADSLRRYSRNSATLQALRARVISAA
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
IYPSVLLVVGMAVVLFLLAFVVPRFAALLEHSGRELPLMSRWLMMWGGAVHRHGSAIALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHCCCHHHHH
FAILLLAGIVALQRQSIRDLLADRLLALPGIGPHFRIYRQSQFFRTSAMLVEGGIPAVHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
FELSQGLVSQSDRAALSTALHAVRSGRKISEAFQQCGLANAITSRLLTVAEKTGGLGPVL
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
DKIAGFQESHVSRAIDLISRLIEPVMMIFIGVVIGGIVVLMYMPIFELASSVQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRGRPAGLTWRTTVTYMLCVFDSGGRVKTIYLDGESAANVVTQAESRGLRVASVRSTVTT
CCCCCCCCCHHHHHHHHHEEECCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHH
TNSLRGWRVLPGVRFDVELFARELAALLDAGVGVIDALRTLGGNDRREASARVYGNLLRQ
CCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LEEGHSLSAAMVNAANVFPDVLIACVKASEQTGGLADSLRRYSRNSATLQALRARVISAA
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
IYPSVLLVVGMAVVLFLLAFVVPRFAALLEHSGRELPLMSRWLMMWGGAVHRHGSAIALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHCCCHHHHH
FAILLLAGIVALQRQSIRDLLADRLLALPGIGPHFRIYRQSQFFRTSAMLVEGGIPAVHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
FELSQGLVSQSDRAALSTALHAVRSGRKISEAFQQCGLANAITSRLLTVAEKTGGLGPVL
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
DKIAGFQESHVSRAIDLISRLIEPVMMIFIGVVIGGIVVLMYMPIFELASSVQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10024587; 10952301 [H]