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The map label for this gene is gspE [H]

Identifier: 94313633

GI number: 94313633

Start: 1353164

End: 1354870

Strand: Reverse

Name: gspE [H]

Synonym: Rmet_4711

Alternate gene names: 94313633

Gene position: 1354870-1353164 (Counterclockwise)

Preceding gene: 94313634

Following gene: 94313632

Centisome position: 52.51

GC content: 64.97

Gene sequence:

>1707_bases
ATGGACCCGATCGGCACGCATCCGGAGCAGCATGGGGGGACCACCCTCCGCGCAACGCTGCGCGAACTCGCGGCGTCCTA
CGGGTCGGGCACCCGCGCGCTGGAAGCGTATATGTCCCAGAGCGGCGTGGGCGCGGACGAGAGCCTTGCCGAGCTGGCAA
GCGCCTTCCGGATGGAGCCCATCGGCATTCGCGACCTGCAGGAGATGGAGCCAGACTTCGAGATCATTCCATTCGTCGAG
GCCGTGGCACGCCTGTGTGTTTGCTTCCGGTCCGGGGACGGGCTCGTGTTCGTGATCGCGGACCCGCTCGATGCGCAGTC
GCGCGGGCGGGTTGACCAGCGGATGCGTGCCCGGCCGCACGTGCGTTACGAATGGCGCCTGGCGACCGTCGGCGACGTGA
CGGCCTATCTTGCGGCGCGTGAGAAAGACGTCCACGCGCTGGATACCGTTGCCTTCGAGGAACTGGAGCACCGCGCGGCG
GAATCGTCCGCGCTGGCGCTGAGTCTGCAGGGCATCAGCAATGACGACAGCTCGGTCGTCAGGTTGCTCAATTCGACCAT
CTATGACGCCTTGAAGATGCAGGCCAGCGATATCCATCTGGAATGCCGCGCTAACGGCCTGGTGATCAAGTGCCGCATCG
ACGGCGTGCTGGCGGTGGTGGGCAGGGTCGACGGACGCGCACCCGCCGATCAGGTGCTGTCGCGTGTCAAGGTCATCTCG
GAACTCGATATCGCCGAGCGGCGCGTGCCGCAGGATGGTCGCTTCAAGGCGCAGTATGCCGGGCGGGAGATCGACTTCCG
CGTATCGATCATGCCGAACCTGTTCGGGGAGGACGCCGTCCTGCGCATTCTGGACCGCTACCAGCTATCGCAGTCCGCGC
AGGGCCTGACGCTCGAAACACTGGGTTTTCAGGCTGCCGATACCGGTTTCATGCGGTCGGTGGCGTCAATGCCTTATGGC
ATGTTGCTGGTGACCGGCCCAACCGGTAGCGGCAAGACCACGACGCTGTACGCCATCCTGACCGAGATAAACGATGGTCT
GGACAAGATCGTGACCATCGAGGACCCGGTCGAGTACCAGCTCGGGGACATCCTGCAGATCCCGGTCAACGAGGCCAAGG
GCCTGACCTTCGCGCGTGGCCTGCGCTCCATCCTGCGTCATGACCCGGACAAGATCATGGTGGGCGAGATCCGCGATGCG
GAGACCGCGCAGATCGCGGTGCAGGCGGCGCTGACCGGACACCAGGTGTTCACGACCGTCCACGCGAACAACGTGTTCGA
CGTGATCGGTCGTTTCACGAACATGGGGGTGGATCCATACAGCTTCGTCTCGGCGCTGAATGGCGTGGTCGCCCAACGGC
TGGTGCGCAAGTGTTGCGATGGCTGCGTGGTCGAGGAAGCCGGTGATCGGGACTTGCTGGCACGATCTGGCATCGACCCG
GACATGGCGGACGGGTTCCGCTTCCGGCGCGGACTGGGTTGCGCGGCCTGCCGTGGCAGTGGCTATCGTGGCCGGCGCGC
GATTGCGGAAGCGCTGCGGATGAACGACGAACTCCGGGCGCTGCTCTCGGAGCGCGCGCCACTTGCGCGCATCAAGAGCG
TGGCACTGCGGCAAGGTATGCAAACGCTGCGCAAGGCCGCGATCGAGATGGCGGCCGCAGGGGAAACAACGCTCGAGGAG
ATCAATCGTGTCACGATCTCCGAGTAA

Upstream 100 bases:

>100_bases
GCCGGTGATGATGATTTTCATCGGCGTCGTGATCGGCGGCATTGTCGTGCTGATGTACATGCCGATCTTCGAGCTCGCCT
CCAGCGTTCAGTAGACCATC

Downstream 100 bases:

>100_bases
CCGTCTTGCCATCGGCATTGGCACGTGCGATGTCGCGGTCGGCGTGTGCCAGTCCAGGGCCGAGCGTCTGTGGACGCTGT
TGCAGCGACGCGAAGTCAAG

Product: type II secretion system protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MDPIGTHPEQHGGTTLRATLRELAASYGSGTRALEAYMSQSGVGADESLAELASAFRMEPIGIRDLQEMEPDFEIIPFVE
AVARLCVCFRSGDGLVFVIADPLDAQSRGRVDQRMRARPHVRYEWRLATVGDVTAYLAAREKDVHALDTVAFEELEHRAA
ESSALALSLQGISNDDSSVVRLLNSTIYDALKMQASDIHLECRANGLVIKCRIDGVLAVVGRVDGRAPADQVLSRVKVIS
ELDIAERRVPQDGRFKAQYAGREIDFRVSIMPNLFGEDAVLRILDRYQLSQSAQGLTLETLGFQAADTGFMRSVASMPYG
MLLVTGPTGSGKTTTLYAILTEINDGLDKIVTIEDPVEYQLGDILQIPVNEAKGLTFARGLRSILRHDPDKIMVGEIRDA
ETAQIAVQAALTGHQVFTTVHANNVFDVIGRFTNMGVDPYSFVSALNGVVAQRLVRKCCDGCVVEEAGDRDLLARSGIDP
DMADGFRFRRGLGCAACRGSGYRGRRAIAEALRMNDELRALLSERAPLARIKSVALRQGMQTLRKAAIEMAAAGETTLEE
INRVTISE

Sequences:

>Translated_568_residues
MDPIGTHPEQHGGTTLRATLRELAASYGSGTRALEAYMSQSGVGADESLAELASAFRMEPIGIRDLQEMEPDFEIIPFVE
AVARLCVCFRSGDGLVFVIADPLDAQSRGRVDQRMRARPHVRYEWRLATVGDVTAYLAAREKDVHALDTVAFEELEHRAA
ESSALALSLQGISNDDSSVVRLLNSTIYDALKMQASDIHLECRANGLVIKCRIDGVLAVVGRVDGRAPADQVLSRVKVIS
ELDIAERRVPQDGRFKAQYAGREIDFRVSIMPNLFGEDAVLRILDRYQLSQSAQGLTLETLGFQAADTGFMRSVASMPYG
MLLVTGPTGSGKTTTLYAILTEINDGLDKIVTIEDPVEYQLGDILQIPVNEAKGLTFARGLRSILRHDPDKIMVGEIRDA
ETAQIAVQAALTGHQVFTTVHANNVFDVIGRFTNMGVDPYSFVSALNGVVAQRLVRKCCDGCVVEEAGDRDLLARSGIDP
DMADGFRFRRGLGCAACRGSGYRGRRAIAEALRMNDELRALLSERAPLARIKSVALRQGMQTLRKAAIEMAAAGETTLEE
INRVTISE
>Mature_568_residues
MDPIGTHPEQHGGTTLRATLRELAASYGSGTRALEAYMSQSGVGADESLAELASAFRMEPIGIRDLQEMEPDFEIIPFVE
AVARLCVCFRSGDGLVFVIADPLDAQSRGRVDQRMRARPHVRYEWRLATVGDVTAYLAAREKDVHALDTVAFEELEHRAA
ESSALALSLQGISNDDSSVVRLLNSTIYDALKMQASDIHLECRANGLVIKCRIDGVLAVVGRVDGRAPADQVLSRVKVIS
ELDIAERRVPQDGRFKAQYAGREIDFRVSIMPNLFGEDAVLRILDRYQLSQSAQGLTLETLGFQAADTGFMRSVASMPYG
MLLVTGPTGSGKTTTLYAILTEINDGLDKIVTIEDPVEYQLGDILQIPVNEAKGLTFARGLRSILRHDPDKIMVGEIRDA
ETAQIAVQAALTGHQVFTTVHANNVFDVIGRFTNMGVDPYSFVSALNGVVAQRLVRKCCDGCVVEEAGDRDLLARSGIDP
DMADGFRFRRGLGCAACRGSGYRGRRAIAEALRMNDELRALLSERAPLARIKSVALRQGMQTLRKAAIEMAAAGETTLEE
INRVTISE

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=401, Percent_Identity=43.3915211970075, Blast_Score=321, Evalue=1e-88,
Organism=Escherichia coli, GI1786296, Length=383, Percent_Identity=39.686684073107, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI87082188, Length=208, Percent_Identity=36.5384615384615, Blast_Score=99, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 61960; Mature: 61960

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPIGTHPEQHGGTTLRATLRELAASYGSGTRALEAYMSQSGVGADESLAELASAFRMEP
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
IGIRDLQEMEPDFEIIPFVEAVARLCVCFRSGDGLVFVIADPLDAQSRGRVDQRMRARPH
CCCHHHHHCCCCCEEEHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCHHHHHHCCCC
VRYEWRLATVGDVTAYLAAREKDVHALDTVAFEELEHRAAESSALALSLQGISNDDSSVV
CEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHH
RLLNSTIYDALKMQASDIHLECRANGLVIKCRIDGVLAVVGRVDGRAPADQVLSRVKVIS
HHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHH
ELDIAERRVPQDGRFKAQYAGREIDFRVSIMPNLFGEDAVLRILDRYQLSQSAQGLTLET
HHHHHHHCCCCCCCEEHEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
LGFQAADTGFMRSVASMPYGMLLVTGPTGSGKTTTLYAILTEINDGLDKIVTIEDPVEYQ
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHCCCCCEEEECCCCCCC
LGDILQIPVNEAKGLTFARGLRSILRHDPDKIMVGEIRDAETAQIAVQAALTGHQVFTTV
CCCEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEE
HANNVFDVIGRFTNMGVDPYSFVSALNGVVAQRLVRKCCDGCVVEEAGDRDLLARSGIDP
ECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHCCCCC
DMADGFRFRRGLGCAACRGSGYRGRRAIAEALRMNDELRALLSERAPLARIKSVALRQGM
CHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHH
QTLRKAAIEMAAAGETTLEEINRVTISE
HHHHHHHHHHHHCCCHHHHHHCHHCCCC
>Mature Secondary Structure
MDPIGTHPEQHGGTTLRATLRELAASYGSGTRALEAYMSQSGVGADESLAELASAFRMEP
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
IGIRDLQEMEPDFEIIPFVEAVARLCVCFRSGDGLVFVIADPLDAQSRGRVDQRMRARPH
CCCHHHHHCCCCCEEEHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCHHHHHHCCCC
VRYEWRLATVGDVTAYLAAREKDVHALDTVAFEELEHRAAESSALALSLQGISNDDSSVV
CEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHH
RLLNSTIYDALKMQASDIHLECRANGLVIKCRIDGVLAVVGRVDGRAPADQVLSRVKVIS
HHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHH
ELDIAERRVPQDGRFKAQYAGREIDFRVSIMPNLFGEDAVLRILDRYQLSQSAQGLTLET
HHHHHHHCCCCCCCEEHEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
LGFQAADTGFMRSVASMPYGMLLVTGPTGSGKTTTLYAILTEINDGLDKIVTIEDPVEYQ
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHCCCCCEEEECCCCCCC
LGDILQIPVNEAKGLTFARGLRSILRHDPDKIMVGEIRDAETAQIAVQAALTGHQVFTTV
CCCEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEE
HANNVFDVIGRFTNMGVDPYSFVSALNGVVAQRLVRKCCDGCVVEEAGDRDLLARSGIDP
ECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHCCCCC
DMADGFRFRRGLGCAACRGSGYRGRRAIAEALRMNDELRALLSERAPLARIKSVALRQGM
CHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHH
QTLRKAAIEMAAAGETTLEEINRVTISE
HHHHHHHHHHHHCCCHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]