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The map label for this gene is plcN2 [H]

Identifier: 94313546

GI number: 94313546

Start: 1250263

End: 1252500

Strand: Direct

Name: plcN2 [H]

Synonym: Rmet_4624

Alternate gene names: 94313546

Gene position: 1250263-1252500 (Clockwise)

Preceding gene: 94313545

Following gene: 94313550

Centisome position: 48.46

GC content: 64.88

Gene sequence:

>2238_bases
ATGATGAATTCGCGCCGCAAGTTCTTGCAGGCCAGCACCGGTGCCGCCTTTTCCGCCGCCGCCCTCGCTGCATTTCCGCC
GAGCATCCGCCGTGCGCTGGCGATTCCCGCCAGCAACGTCACGGGAACGATCAACGACGTTCAGCACGTGGTGATCCTGA
TGCAGGAAAACCGCTCGTTCGACCACTACTTCGGCACGCTCAACGGTGTGCGTGGCTTCGGTGACCGGATCACGATTCCG
ATGCCCAATGGCCGCAAGGTCTGGCAGCAGGAGCGCGCTAACGGCACCGTCATCACGCCCTACCACCTCGATGGCACGGC
CAACAACGCCCAACGCACGTCGGGCACGCCGCACGCGTGGGCGGACAGCCAGCAGGCCTGGGACAACGGTCGCATGTCCC
GCTGGCCAGTGGCCAAAACCGACACGTCGATGGGCTACTTCAAGGAGCAAGAGATCCCGTACCAGTTCGCGCTGGCCAAT
GCCTTCACGATCTGCGACGCATATCACTGCTCGATGCACACCGGCACCGACGCGAACCGCTCGTTCCATATCTCGGGGAC
CAACGGCGCGACGCCGCAGAACGTGGCTTTCGTCAACAACGAGTGGGATGCGATCAATGGCGTGCCGGCCGACGCAAACA
TCGGCTATACGTGGAAGACCTATGCCGAGCGGCTGGAAGAAGCCGGCGTGAGCTGGATCAGCTACCAGAACATGCCCGAC
GAATGGGGCGACAACATGCTTGGCGCGTTCCAGACGTTCCGGCGCGCGAACCTGGCATCGGGCTTTCCGGTCTCCAGCGG
CGGCTCGCCGGGCAAGCCCTACACCAATACCGGCCAGGCAGTGCCGTATCACGCCTATGACGCCGCCACCGACAATACCG
CCAATCCGCTGTACAAGGGCGTGGCGAACACGCTGCCGGGCGACAAGCCGGACCAGTATTTGGACGCTTTCAGGAAGGCG
GTTCTCGACAACAAGCTGCCGCAGGTGTCGTGGATCAACGCACCGTCGATCTACTGCGAACATCCGTCGCCATCCAGCCC
AGTGCAAGGCGCGTGGTTCCTGCAGGAGGTACTGGACGCCCTGACTGCGAATCCGGACGTCTGGAGCAAGACCGTTCTGC
TGATCAACTTTGATGAGAACGACGGCTACTTCGACCATGTGCCCTCGCCGTCGGCACCGTCGAAGAACCCGGATGGCACC
TATGCGGGCAAGACCACGCTGGCGGATGCGGACCTGACCGCCGAGTACTTCACGCAAGGCGCGCCGGCCGGCAGCACGGG
CCAGCCCGCGCCGGATGGCCGCGTCTTCGGCCCCGGTCCGCGGGTGCCGCTTTATGTGATCTCGCCGTGGAGCCGCGGCG
GGTGGGTCAATTCGCAGGTGTTCGATCATACGTCGGTGCTGCGCTTCCTTGAGGCCCGATTCGGCGTGCGCGAGCCGAAC
ATCAGCCCGTTCCGCCGCGCGGTCTGCGGCGACCTGACCAGCGCGTTCAACTTCAAGACGCCGAACAACGAGCCCATGCC
CACGCTGGGCGGCCGCACCACGCGAGAGCTTGCCGACAAGCTGCGCGCGGACCAGCAGAAGCTGCAGCAGGTGCCGGTGC
CACGCGACATGCAGTTGCCGCTGCAGGCCGCCGGTGCGCGTCCGTCGCGGGCCTTGCCGTATGAGCTGCATACCAGCGCG
CGTTGCCGTGCTTCCGGTCAGGTCGAACTGGTATTCGCGAACTCGGGCACGCAGGCTGCCGTCTTCCACGTGTACGACGG
TTTCAAGCTTGATCGCATCCCGCGCCGCTATGCCATCGAGGCGGGCAAGACCTTGTCGGACACCTGGGACGCCGCACGTG
ACAACTTCGGCAAGTACGACCTATGGGTGCTGGGCCCGAACGGCTTCCATCGGCATTTCAAGGGCGACCTGAACCGCCTG
ACCGGCACGCAGGCCGCGCCGGAAGTCCGCGTGTGCTACGACGTAGCCAATGGCAATGTCTACGTGGACCTGATGAACAA
GGGCGGCCAGCCGTGCGTGTTCACCGTGACGCCAGTGGCCTACCGCCAGGACGGTCCGTGGACCGCGAATGTGGCCGCCG
GCGCCAGCACCACCCAGCACTGGGAGCTGCAGGCCAGCGGGCAGTGGTATGACTTCGCCGTGACGTGCAGCAGTGATGCG
TCGTTCTATCGCCGCTTCTCTGGCCGTGTGGAGACTGGCAAGCACGCCACCAGCGATCCGGCCATGAGCGAGGCGTAA

Upstream 100 bases:

>100_bases
CCTCTACACCCGACGGCGCTCCCAACCCCACCGAGCCGGCCAAGCCGGACATGCGCTGCGCGCCCTGAACGCGCGCACGG
CTCAACCCTCACTCGATGAC

Downstream 100 bases:

>100_bases
CGCGTCGACAAGCGGTGGTCCGTAAGCCGCCGCTTGCCAACGCACGCCGCCGCGCCAGTTACTGCACCGAGTACCCGCGG
CCCGTCATGCAGGCGCGCCA

Product: phospholipase C (phosphatidylcholine cholinephosphohydrolase) signal peptide protein

Products: NA

Alternate protein names: PLC-N; Phosphatidylcholine cholinephosphohydrolase; Phosphatidylcholine-hydrolyzing phospholipase C; PC-PLC [H]

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MMNSRRKFLQASTGAAFSAAALAAFPPSIRRALAIPASNVTGTINDVQHVVILMQENRSFDHYFGTLNGVRGFGDRITIP
MPNGRKVWQQERANGTVITPYHLDGTANNAQRTSGTPHAWADSQQAWDNGRMSRWPVAKTDTSMGYFKEQEIPYQFALAN
AFTICDAYHCSMHTGTDANRSFHISGTNGATPQNVAFVNNEWDAINGVPADANIGYTWKTYAERLEEAGVSWISYQNMPD
EWGDNMLGAFQTFRRANLASGFPVSSGGSPGKPYTNTGQAVPYHAYDAATDNTANPLYKGVANTLPGDKPDQYLDAFRKA
VLDNKLPQVSWINAPSIYCEHPSPSSPVQGAWFLQEVLDALTANPDVWSKTVLLINFDENDGYFDHVPSPSAPSKNPDGT
YAGKTTLADADLTAEYFTQGAPAGSTGQPAPDGRVFGPGPRVPLYVISPWSRGGWVNSQVFDHTSVLRFLEARFGVREPN
ISPFRRAVCGDLTSAFNFKTPNNEPMPTLGGRTTRELADKLRADQQKLQQVPVPRDMQLPLQAAGARPSRALPYELHTSA
RCRASGQVELVFANSGTQAAVFHVYDGFKLDRIPRRYAIEAGKTLSDTWDAARDNFGKYDLWVLGPNGFHRHFKGDLNRL
TGTQAAPEVRVCYDVANGNVYVDLMNKGGQPCVFTVTPVAYRQDGPWTANVAAGASTTQHWELQASGQWYDFAVTCSSDA
SFYRRFSGRVETGKHATSDPAMSEA

Sequences:

>Translated_745_residues
MMNSRRKFLQASTGAAFSAAALAAFPPSIRRALAIPASNVTGTINDVQHVVILMQENRSFDHYFGTLNGVRGFGDRITIP
MPNGRKVWQQERANGTVITPYHLDGTANNAQRTSGTPHAWADSQQAWDNGRMSRWPVAKTDTSMGYFKEQEIPYQFALAN
AFTICDAYHCSMHTGTDANRSFHISGTNGATPQNVAFVNNEWDAINGVPADANIGYTWKTYAERLEEAGVSWISYQNMPD
EWGDNMLGAFQTFRRANLASGFPVSSGGSPGKPYTNTGQAVPYHAYDAATDNTANPLYKGVANTLPGDKPDQYLDAFRKA
VLDNKLPQVSWINAPSIYCEHPSPSSPVQGAWFLQEVLDALTANPDVWSKTVLLINFDENDGYFDHVPSPSAPSKNPDGT
YAGKTTLADADLTAEYFTQGAPAGSTGQPAPDGRVFGPGPRVPLYVISPWSRGGWVNSQVFDHTSVLRFLEARFGVREPN
ISPFRRAVCGDLTSAFNFKTPNNEPMPTLGGRTTRELADKLRADQQKLQQVPVPRDMQLPLQAAGARPSRALPYELHTSA
RCRASGQVELVFANSGTQAAVFHVYDGFKLDRIPRRYAIEAGKTLSDTWDAARDNFGKYDLWVLGPNGFHRHFKGDLNRL
TGTQAAPEVRVCYDVANGNVYVDLMNKGGQPCVFTVTPVAYRQDGPWTANVAAGASTTQHWELQASGQWYDFAVTCSSDA
SFYRRFSGRVETGKHATSDPAMSEA
>Mature_745_residues
MMNSRRKFLQASTGAAFSAAALAAFPPSIRRALAIPASNVTGTINDVQHVVILMQENRSFDHYFGTLNGVRGFGDRITIP
MPNGRKVWQQERANGTVITPYHLDGTANNAQRTSGTPHAWADSQQAWDNGRMSRWPVAKTDTSMGYFKEQEIPYQFALAN
AFTICDAYHCSMHTGTDANRSFHISGTNGATPQNVAFVNNEWDAINGVPADANIGYTWKTYAERLEEAGVSWISYQNMPD
EWGDNMLGAFQTFRRANLASGFPVSSGGSPGKPYTNTGQAVPYHAYDAATDNTANPLYKGVANTLPGDKPDQYLDAFRKA
VLDNKLPQVSWINAPSIYCEHPSPSSPVQGAWFLQEVLDALTANPDVWSKTVLLINFDENDGYFDHVPSPSAPSKNPDGT
YAGKTTLADADLTAEYFTQGAPAGSTGQPAPDGRVFGPGPRVPLYVISPWSRGGWVNSQVFDHTSVLRFLEARFGVREPN
ISPFRRAVCGDLTSAFNFKTPNNEPMPTLGGRTTRELADKLRADQQKLQQVPVPRDMQLPLQAAGARPSRALPYELHTSA
RCRASGQVELVFANSGTQAAVFHVYDGFKLDRIPRRYAIEAGKTLSDTWDAARDNFGKYDLWVLGPNGFHRHFKGDLNRL
TGTQAAPEVRVCYDVANGNVYVDLMNKGGQPCVFTVTPVAYRQDGPWTANVAAGASTTQHWELQASGQWYDFAVTCSSDA
SFYRRFSGRVETGKHATSDPAMSEA

Specific function: Hydrolyzes phosphatidylserine as well as phosphatidylcholine [H]

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007312
- InterPro:   IPR008475
- InterPro:   IPR017767
- InterPro:   IPR006311 [H]

Pfam domain/function: PF05506 DUF756; PF04185 Phosphoesterase [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 81621; Mature: 81621

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNSRRKFLQASTGAAFSAAALAAFPPSIRRALAIPASNVTGTINDVQHVVILMQENRSF
CCCCHHHHHHHCCCCHHHHHHHHHCCHHHHHEEECCCCCCCCCHHCCEEEEEEEECCCCH
DHYFGTLNGVRGFGDRITIPMPNGRKVWQQERANGTVITPYHLDGTANNAQRTSGTPHAW
HHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCC
ADSQQAWDNGRMSRWPVAKTDTSMGYFKEQEIPYQFALANAFTICDAYHCSMHTGTDANR
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHEEEECCCCCCCCC
SFHISGTNGATPQNVAFVNNEWDAINGVPADANIGYTWKTYAERLEEAGVSWISYQNMPD
EEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCH
EWGDNMLGAFQTFRRANLASGFPVSSGGSPGKPYTNTGQAVPYHAYDAATDNTANPLYKG
HHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHH
VANTLPGDKPDQYLDAFRKAVLDNKLPQVSWINAPSIYCEHPSPSSPVQGAWFLQEVLDA
HHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCCCHHHHHHHHHHH
LTANPDVWSKTVLLINFDENDGYFDHVPSPSAPSKNPDGTYAGKTTLADADLTAEYFTQG
HCCCCCCCCEEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHCC
APAGSTGQPAPDGRVFGPGPRVPLYVISPWSRGGWVNSQVFDHTSVLRFLEARFGVREPN
CCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
ISPFRRAVCGDLTSAFNFKTPNNEPMPTLGGRTTRELADKLRADQQKLQQVPVPRDMQLP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LQAAGARPSRALPYELHTSARCRASGQVELVFANSGTQAAVFHVYDGFKLDRIPRRYAIE
HHHCCCCCCCCCCEEECCCCEECCCCCEEEEEECCCCCEEEEEEECCCEECCCCHHHHHH
AGKTLSDTWDAARDNFGKYDLWVLGPNGFHRHFKGDLNRLTGTQAAPEVRVCYDVANGNV
CCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCE
YVDLMNKGGQPCVFTVTPVAYRQDGPWTANVAAGASTTQHWELQASGQWYDFAVTCSSDA
EEEEECCCCCCEEEEECCEEEECCCCEEEEEECCCCCCEEEEEEECCCEEEEEEEECCCH
SFYRRFSGRVETGKHATSDPAMSEA
HHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMNSRRKFLQASTGAAFSAAALAAFPPSIRRALAIPASNVTGTINDVQHVVILMQENRSF
CCCCHHHHHHHCCCCHHHHHHHHHCCHHHHHEEECCCCCCCCCHHCCEEEEEEEECCCCH
DHYFGTLNGVRGFGDRITIPMPNGRKVWQQERANGTVITPYHLDGTANNAQRTSGTPHAW
HHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCC
ADSQQAWDNGRMSRWPVAKTDTSMGYFKEQEIPYQFALANAFTICDAYHCSMHTGTDANR
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHEEEECCCCCCCCC
SFHISGTNGATPQNVAFVNNEWDAINGVPADANIGYTWKTYAERLEEAGVSWISYQNMPD
EEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCH
EWGDNMLGAFQTFRRANLASGFPVSSGGSPGKPYTNTGQAVPYHAYDAATDNTANPLYKG
HHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHH
VANTLPGDKPDQYLDAFRKAVLDNKLPQVSWINAPSIYCEHPSPSSPVQGAWFLQEVLDA
HHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCCCHHHHHHHHHHH
LTANPDVWSKTVLLINFDENDGYFDHVPSPSAPSKNPDGTYAGKTTLADADLTAEYFTQG
HCCCCCCCCEEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHCC
APAGSTGQPAPDGRVFGPGPRVPLYVISPWSRGGWVNSQVFDHTSVLRFLEARFGVREPN
CCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
ISPFRRAVCGDLTSAFNFKTPNNEPMPTLGGRTTRELADKLRADQQKLQQVPVPRDMQLP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LQAAGARPSRALPYELHTSARCRASGQVELVFANSGTQAAVFHVYDGFKLDRIPRRYAIE
HHHCCCCCCCCCCEEECCCCEECCCCCEEEEEECCCCCEEEEEEECCCEECCCCHHHHHH
AGKTLSDTWDAARDNFGKYDLWVLGPNGFHRHFKGDLNRLTGTQAAPEVRVCYDVANGNV
CCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCE
YVDLMNKGGQPCVFTVTPVAYRQDGPWTANVAAGASTTQHWELQASGQWYDFAVTCSSDA
EEEEECCCCCCEEEEECCEEEECCCCEEEEEECCCCCCEEEEEEECCCEEEEEEEECCCH
SFYRRFSGRVETGKHATSDPAMSEA
HHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2120196; 10984043 [H]