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The map label for this gene is lutR [H]

Identifier: 94313525

GI number: 94313525

Start: 1224823

End: 1225659

Strand: Direct

Name: lutR [H]

Synonym: Rmet_4603

Alternate gene names: 94313525

Gene position: 1224823-1225659 (Clockwise)

Preceding gene: 94313524

Following gene: 94313526

Centisome position: 47.47

GC content: 61.53

Gene sequence:

>837_bases
ATGTCGATGTTCCGCGAAGCGAAGGAAGGTGCCATTGGAATCCGCAAGCAAAAACGTGCGGACCTCGTGGCTGAAGAACT
GAAGCGGCTGATTACCCAGCGCAACCTCAAGCCTGGCGACAAGCTGCCTCACGAGATCAAGCTTCAGCAGATGTTCTCGG
TCAGCAAGAGCACGATTCGGGAGGCGCTGAAGTCGTTGGAAGTCCAGGGGCTGATCAGTGTCAGCACCGGGCCCTCGGGC
GGTGCCACGATCGTTGAAGTACCGCTGGACCGCACCTTCCAGCTCATGCAGAACTATCTTTTCTTCAAGGACGTTGGCAT
TGCCGATGTCTACACGGTCCGCCGGCTCCTGGAGCCCGAGCTGGCCGCCGGCGCGATTCCGCATTTGACCGAGGATGATT
TTCTGGCGCTCGAGCGCACGATCGACTTGTGCGACCCGGCGTCACAGGCTGCCGCCGGCACCAAGATGCTGGACCAGCGA
CAGGAGGACCTGACCTTCCACGACATCCTGGCCGCGGCGAACCCGAATCCGATGCTGCGTTTTTGCTGCGAACTCATCAA
CGAGATGATTCGCCAGCTAGTCGTGTTCGGCAACGAGACTCCGGCGTGCGAGCACGAACGTTTCGGGGCCGCCAACGTGG
TGTTCCACCGGCGGATCGTGGCGGCTGCGCGCGCGCGCGATGCGGAGGCGGTGCGCTCGCTGATGTCGGCGCACATGGAA
GAGTGCACGCATTACGTTACGCGCATGAACGGCCGTGTGCATGGCAGGCTGGTCCTGGATTCCGAGATGACTCGGCGGAC
ATGGCTGAAACCGAGTGAGACGGCCGACAAGGCTTGA

Upstream 100 bases:

>100_bases
TTTCGTCCGGATGCCGCAGCCAGGCCCCAGATGGATAGAATGGCGCCCAGTGATCGACTGGGCGAGGCATGCAGGTCCAG
CAAACGTGAAGGATTGCGCA

Downstream 100 bases:

>100_bases
TCAGCCTCGTCGGTCGTGCACGATGCTCTGGCGTCCTACAGATAGGTGATGGTGACGATGTAGCCGGCTGGCCGGGTATC
GGTCGTGCTGGTGCTGGCGC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSMFREAKEGAIGIRKQKRADLVAEELKRLITQRNLKPGDKLPHEIKLQQMFSVSKSTIREALKSLEVQGLISVSTGPSG
GATIVEVPLDRTFQLMQNYLFFKDVGIADVYTVRRLLEPELAAGAIPHLTEDDFLALERTIDLCDPASQAAAGTKMLDQR
QEDLTFHDILAAANPNPMLRFCCELINEMIRQLVVFGNETPACEHERFGAANVVFHRRIVAAARARDAEAVRSLMSAHME
ECTHYVTRMNGRVHGRLVLDSEMTRRTWLKPSETADKA

Sequences:

>Translated_278_residues
MSMFREAKEGAIGIRKQKRADLVAEELKRLITQRNLKPGDKLPHEIKLQQMFSVSKSTIREALKSLEVQGLISVSTGPSG
GATIVEVPLDRTFQLMQNYLFFKDVGIADVYTVRRLLEPELAAGAIPHLTEDDFLALERTIDLCDPASQAAAGTKMLDQR
QEDLTFHDILAAANPNPMLRFCCELINEMIRQLVVFGNETPACEHERFGAANVVFHRRIVAAARARDAEAVRSLMSAHME
ECTHYVTRMNGRVHGRLVLDSEMTRRTWLKPSETADKA
>Mature_277_residues
SMFREAKEGAIGIRKQKRADLVAEELKRLITQRNLKPGDKLPHEIKLQQMFSVSKSTIREALKSLEVQGLISVSTGPSGG
ATIVEVPLDRTFQLMQNYLFFKDVGIADVYTVRRLLEPELAAGAIPHLTEDDFLALERTIDLCDPASQAAAGTKMLDQRQ
EDLTFHDILAAANPNPMLRFCCELINEMIRQLVVFGNETPACEHERFGAANVVFHRRIVAAARARDAEAVRSLMSAHMEE
CTHYVTRMNGRVHGRLVLDSEMTRRTWLKPSETADKA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789621, Length=175, Percent_Identity=26.2857142857143, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790780, Length=234, Percent_Identity=24.3589743589744, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 31201; Mature: 31070

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFREAKEGAIGIRKQKRADLVAEELKRLITQRNLKPGDKLPHEIKLQQMFSVSKSTIR
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
EALKSLEVQGLISVSTGPSGGATIVEVPLDRTFQLMQNYLFFKDVGIADVYTVRRLLEPE
HHHHHHCHHHEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHH
LAAGAIPHLTEDDFLALERTIDLCDPASQAAAGTKMLDQRQEDLTFHDILAAANPNPMLR
HHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHH
FCCELINEMIRQLVVFGNETPACEHERFGAANVVFHRRIVAAARARDAEAVRSLMSAHME
HHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ECTHYVTRMNGRVHGRLVLDSEMTRRTWLKPSETADKA
HHHHHHHHHCCCEEEEEEECCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SMFREAKEGAIGIRKQKRADLVAEELKRLITQRNLKPGDKLPHEIKLQQMFSVSKSTIR
CHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
EALKSLEVQGLISVSTGPSGGATIVEVPLDRTFQLMQNYLFFKDVGIADVYTVRRLLEPE
HHHHHHCHHHEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHH
LAAGAIPHLTEDDFLALERTIDLCDPASQAAAGTKMLDQRQEDLTFHDILAAANPNPMLR
HHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHH
FCCELINEMIRQLVVFGNETPACEHERFGAANVVFHRRIVAAARARDAEAVRSLMSAHME
HHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ECTHYVTRMNGRVHGRLVLDSEMTRRTWLKPSETADKA
HHHHHHHHHCCCEEEEEEECCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]