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The map label for this gene is ilvD [H]

Identifier: 94313507

GI number: 94313507

Start: 1202465

End: 1204327

Strand: Reverse

Name: ilvD [H]

Synonym: Rmet_4585

Alternate gene names: 94313507

Gene position: 1204327-1202465 (Counterclockwise)

Preceding gene: 94313508

Following gene: 94313503

Centisome position: 46.68

GC content: 64.2

Gene sequence:

>1863_bases
ATGCCTGAGTACCGATCACGAACTTCGACGCACGGCCGGAACATGGCGGGTGCTCGCGCCTTGTGGCGCGCCACCGGGAT
GAAGGATGGCGATTTCGGCAAGCCCATCATCGCCGTGGTGAACTCGTTTACCCAGTTCGTGCCGGGCCATGTCCATTTGC
GCGACCTCGGCGCGCTGGTTGCGAAGGAGATCGAAGCGGCCGGCGGCGTGGCCAAGGAATTCAACACGATCGCGGTGGAC
GATGGGATCGCCATGGGCCACGGAGGCATGCTCTATTCGCTGCCCTCGCGCGAGCTGATCGCCGACTCGGTGGAATACAT
GGTCAACGCGCATTGCGCCGACGCCATGGTCTGTATTTCCAATTGCGACAAGATCACCCCGGGCATGCTGATGGCCGCGA
TGCGGCTGAACATTCCCGTGGTGTTCGTCTCCGGCGGCCCGATGGAGGCCGGCAAGGTCAAGTCCCCAACGGACGGCCAG
GTGATCGCAAAGATCGACCTGATCGACGCCATGATCAAGGCCGCGGACCCGAAGGTCAGCGACGCTGAAGTGGCCGAAGT
GGAGCGCAGCGCCTGCCCGACATGCGGTTCCTGCTCGGGGATGTTCACGGCGAACTCGATGAATTGCCTGACCGAGGCGA
TTGGCCTGGCGTTGCCGGGCAACGGCACGATCGTTGCCACCCACACATGGCGCAAGGGCCTGTTCGAACAGGCGGGCCGC
CTGGTCGTGGACCTGTGCCGCCGCTACTACCAGGAAGAGGACGCATCGGTCCTGCCGCGCAACATCGCTACCAAGTCGGC
ATTCGAGAATGCCATGGCACTGGACGTGGCCATGGGCGGTTCGACCAATACCGTGCTGCACCTGCTGGCGGCAGCCCAGG
AAGCCGGTGTCGATTTCACGATGTCCGACATCGATCGCATCTCGCGGAAGGTGCCGTGCCTGTGCAAGGCCGCACCGGCC
ACCGACAAGTACCACATCGAAGACGTGCATCGCGCGGGCGGCATCCTTGGCATTCTCGGCGAGCTGGCGCGTGCCGAGTT
GCTGGATCTGTCGTGCGGCAACGTGCATAGCGGCACGCTTGGCAATGCCATTTCCCAGTGGGATGTCGTTGGTGGTGCGG
GCGAGGACGCGCAGAAGTTCTTCCGCGCTGCGCCGGGCGGCATTCCGACGACCGTCGCGTTCAGCCAGGCGGCCACCTTC
GAGTCACTGGATACCGACCGAAAGACTGGCTGCATCCGGAACAAGCAGAACGCGTATTCGAAGGACGGCGGTCTCGCCGT
GCTCTACGGCAACCTCGCGGAGAAGGGCTGCATCGTCAAGACCGCTGGCGTGGACGAATCGCAATGGGTCTTCTCCGGGC
GCGCCCGTGTCTTCGAAAGCCAGGACGAAGCCGTCGACGCCATTCTGGGCGACAAGGTCGTGGCTGGTGACGTTGTCGTG
ATTCGTTACGAAGGTCCCAAGGGTGGCCCGGGCATGCAGGAAATGCTCTATCCCACGTCGTACCTGAAGTCGAAGGGCCT
GGGCAAGACCTGCGCGCTGTTTACCGACGGTCGGTTCTCCGGTGGGTCGTCCGGGCTGGTCATCGGACATGCCTCGCCGG
AAGCCGCCGAAGGCGGCACGATCGGCCTGGTGGAGGACGGCGATCTCATCGAGATCGACATTCCGCAGCGCAAGATGCAC
CTGGCGGTGTCGGACGAAACCCTGGCGCGCCGTCGCGAAGCCATGGAGGCGCGCGGTGACAAGGCGTGGGAGCCGGTGGA
TCGTGTGCGCGTAGTGTCCCAGGCATTGCAGGCGTACGCTGCGCTCGCTACTTCGGCGGATCGCGGCGCGGTGCGCGATA
TCTCCCAGCTCAAGCGCAAGTAA

Upstream 100 bases:

>100_bases
GTGCGGCTGGCGGCCATGGCTTGCCCGGCCGCCGCATACCGGCGCCCTGGCTGGCCTGTGTCCAATTACAACCCCACAAC
TGCAAGATCCGAGGAAAGAG

Downstream 100 bases:

>100_bases
GGCGCGCGCGGCGCGAAGCAGGTCGTTTCGTCCTGCTTCGCCGTGCGCCGCTGAATGGTCGCTGAATGGCACAAAAAAAG
CCTCGCATCTGCGAGGCTTT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 1 [H]

Number of amino acids: Translated: 620; Mature: 619

Protein sequence:

>620_residues
MPEYRSRTSTHGRNMAGARALWRATGMKDGDFGKPIIAVVNSFTQFVPGHVHLRDLGALVAKEIEAAGGVAKEFNTIAVD
DGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVKSPTDGQ
VIAKIDLIDAMIKAADPKVSDAEVAEVERSACPTCGSCSGMFTANSMNCLTEAIGLALPGNGTIVATHTWRKGLFEQAGR
LVVDLCRRYYQEEDASVLPRNIATKSAFENAMALDVAMGGSTNTVLHLLAAAQEAGVDFTMSDIDRISRKVPCLCKAAPA
TDKYHIEDVHRAGGILGILGELARAELLDLSCGNVHSGTLGNAISQWDVVGGAGEDAQKFFRAAPGGIPTTVAFSQAATF
ESLDTDRKTGCIRNKQNAYSKDGGLAVLYGNLAEKGCIVKTAGVDESQWVFSGRARVFESQDEAVDAILGDKVVAGDVVV
IRYEGPKGGPGMQEMLYPTSYLKSKGLGKTCALFTDGRFSGGSSGLVIGHASPEAAEGGTIGLVEDGDLIEIDIPQRKMH
LAVSDETLARRREAMEARGDKAWEPVDRVRVVSQALQAYAALATSADRGAVRDISQLKRK

Sequences:

>Translated_620_residues
MPEYRSRTSTHGRNMAGARALWRATGMKDGDFGKPIIAVVNSFTQFVPGHVHLRDLGALVAKEIEAAGGVAKEFNTIAVD
DGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVKSPTDGQ
VIAKIDLIDAMIKAADPKVSDAEVAEVERSACPTCGSCSGMFTANSMNCLTEAIGLALPGNGTIVATHTWRKGLFEQAGR
LVVDLCRRYYQEEDASVLPRNIATKSAFENAMALDVAMGGSTNTVLHLLAAAQEAGVDFTMSDIDRISRKVPCLCKAAPA
TDKYHIEDVHRAGGILGILGELARAELLDLSCGNVHSGTLGNAISQWDVVGGAGEDAQKFFRAAPGGIPTTVAFSQAATF
ESLDTDRKTGCIRNKQNAYSKDGGLAVLYGNLAEKGCIVKTAGVDESQWVFSGRARVFESQDEAVDAILGDKVVAGDVVV
IRYEGPKGGPGMQEMLYPTSYLKSKGLGKTCALFTDGRFSGGSSGLVIGHASPEAAEGGTIGLVEDGDLIEIDIPQRKMH
LAVSDETLARRREAMEARGDKAWEPVDRVRVVSQALQAYAALATSADRGAVRDISQLKRK
>Mature_619_residues
PEYRSRTSTHGRNMAGARALWRATGMKDGDFGKPIIAVVNSFTQFVPGHVHLRDLGALVAKEIEAAGGVAKEFNTIAVDD
GIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVKSPTDGQV
IAKIDLIDAMIKAADPKVSDAEVAEVERSACPTCGSCSGMFTANSMNCLTEAIGLALPGNGTIVATHTWRKGLFEQAGRL
VVDLCRRYYQEEDASVLPRNIATKSAFENAMALDVAMGGSTNTVLHLLAAAQEAGVDFTMSDIDRISRKVPCLCKAAPAT
DKYHIEDVHRAGGILGILGELARAELLDLSCGNVHSGTLGNAISQWDVVGGAGEDAQKFFRAAPGGIPTTVAFSQAATFE
SLDTDRKTGCIRNKQNAYSKDGGLAVLYGNLAEKGCIVKTAGVDESQWVFSGRARVFESQDEAVDAILGDKVVAGDVVVI
RYEGPKGGPGMQEMLYPTSYLKSKGLGKTCALFTDGRFSGGSSGLVIGHASPEAAEGGTIGLVEDGDLIEIDIPQRKMHL
AVSDETLARRREAMEARGDKAWEPVDRVRVVSQALQAYAALATSADRGAVRDISQLKRK

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=617, Percent_Identity=75.0405186385737, Blast_Score=952, Evalue=0.0,
Organism=Escherichia coli, GI2367371, Length=517, Percent_Identity=31.7214700193424, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1786464, Length=547, Percent_Identity=31.8098720292505, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788157, Length=540, Percent_Identity=28.8888888888889, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322476, Length=611, Percent_Identity=37.8068739770867, Blast_Score=342, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 65651; Mature: 65519

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEYRSRTSTHGRNMAGARALWRATGMKDGDFGKPIIAVVNSFTQFVPGHVHLRDLGALV
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AKEIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHHCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVKSPTDGQVIAKIDLIDAMIKAADPKVS
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCC
DAEVAEVERSACPTCGSCSGMFTANSMNCLTEAIGLALPGNGTIVATHTWRKGLFEQAGR
CHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCEEECCCCEEEEECHHHHHHHHHHHH
LVVDLCRRYYQEEDASVLPRNIATKSAFENAMALDVAMGGSTNTVLHLLAAAQEAGVDFT
HHHHHHHHHHCCCCCHHCCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHCCCCEE
MSDIDRISRKVPCLCKAAPATDKYHIEDVHRAGGILGILGELARAELLDLSCGNVHSGTL
HHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCH
GNAISQWDVVGGAGEDAQKFFRAAPGGIPTTVAFSQAATFESLDTDRKTGCIRNKQNAYS
HCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCHHHCCCCCCCCCCC
KDGGLAVLYGNLAEKGCIVKTAGVDESQWVFSGRARVFESQDEAVDAILGDKVVAGDVVV
CCCCEEEEECCCCCCCCEEEECCCCCCCEEECCCHHEECCHHHHHHHHHCCEEEECCEEE
IRYEGPKGGPGMQEMLYPTSYLKSKGLGKTCALFTDGRFSGGSSGLVIGHASPEAAEGGT
EEECCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCE
IGLVEDGDLIEIDIPQRKMHLAVSDETLARRREAMEARGDKAWEPVDRVRVVSQALQAYA
EEEEECCCEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ALATSADRGAVRDISQLKRK
HHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PEYRSRTSTHGRNMAGARALWRATGMKDGDFGKPIIAVVNSFTQFVPGHVHLRDLGALV
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AKEIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHHCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVKSPTDGQVIAKIDLIDAMIKAADPKVS
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCC
DAEVAEVERSACPTCGSCSGMFTANSMNCLTEAIGLALPGNGTIVATHTWRKGLFEQAGR
CHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCEEECCCCEEEEECHHHHHHHHHHHH
LVVDLCRRYYQEEDASVLPRNIATKSAFENAMALDVAMGGSTNTVLHLLAAAQEAGVDFT
HHHHHHHHHHCCCCCHHCCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHCCCCEE
MSDIDRISRKVPCLCKAAPATDKYHIEDVHRAGGILGILGELARAELLDLSCGNVHSGTL
HHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCH
GNAISQWDVVGGAGEDAQKFFRAAPGGIPTTVAFSQAATFESLDTDRKTGCIRNKQNAYS
HCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCHHHCCCCCCCCCCC
KDGGLAVLYGNLAEKGCIVKTAGVDESQWVFSGRARVFESQDEAVDAILGDKVVAGDVVV
CCCCEEEEECCCCCCCCEEEECCCCCCCEEECCCHHEECCHHHHHHHHHCCEEEECCEEE
IRYEGPKGGPGMQEMLYPTSYLKSKGLGKTCALFTDGRFSGGSSGLVIGHASPEAAEGGT
EEECCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCE
IGLVEDGDLIEIDIPQRKMHLAVSDETLARRREAMEARGDKAWEPVDRVRVVSQALQAYA
EEEEECCCEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ALATSADRGAVRDISQLKRK
HHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]