BacMap

Definition	Cupriavidus metallidurans CH34 megaplasmid, complete sequence.
Accession	NC_007974
Length	2,580,084

Click here to switch to the map view.

The map label for this gene is ygfF [C]

Identifier: 94313469

GI number: 94313469

Start: 1150633

End: 1151397

Strand: Direct

Name: ygfF [C]

Synonym: Rmet_4544

Alternate gene names: 94313469

Gene position: 1150633-1151397 (Clockwise)

Preceding gene: 94313465

Following gene: 94313473

Centisome position: 44.6

GC content: 63.79

Gene sequence:

>765_bases
ATGACTACAGACCACTCGCAGCCGTCGCAGGGGGAATCACAAAAGGCCGCCATCGTTACCGGCGCATCACGCGGCATCGG
CGCCGCCATTTCGGCGCGGCTGGCCCGCGACGGCTTCGCGGTCGTCATCAACTATGCGAACGGCGCGGACACCGCCGGGG
ATCTGCGTCGCAAGCTCGAAGCAAGTGGCGGCCGGGCCATGACCTTCCAGGCCGATGTCAGCGATGCCAGCGCGGTGGCC
CGCATGTTCGATGCAGCCGAGGCAACGTTCGACGGCGTCGACGTTGTCGTCAACAACGCGGGCGTGATGGTGCTCGACAC
AATTGCAGCGTCTGACGACGCAGCATTCGAGCGCGTGATGAACATCAATCTGAAAGGGGCTTTCCACACCATGCGCGAGG
CTGCCCGCCGCCTGCGCCATGGCGGCCGGATCATCAATCTCTCGTCCAGCGTCATTGGTTTGCGCATGGAGCGCTATGGC
ATCTACGCGGCCTCGAAGGCGGCCGTGGAAGCGATGACCGCCGTATTGAGCAAGGAGATGCGCGGCCGCGCCATTACCGT
CAATGCGGTGGCACCCGGGCCGACCGCCACCGACCTGTTCCTCGACGGCAAATCACCTGAAACGATCGAACGGCTCGCGA
AGCTCAATCCGCTCGAACGACTGGGCACACCTGAAGATATAGCGGCAGCGGTGGCATTCCTGGCCAGCCCCGATGGAGCC
TGGATCAATGGCCAGGTGTTACGCGCGAATGGGGGAATGGTCTAG

Upstream 100 bases:

>100_bases
GTTCGGATTTTCGAATATTGATGCCGAATTCTGCCTGTTTATCTAGCATGGCGTAAGGTCTATCGTTCCCTCACCGATTC
AGTTCATTCGAGGAAATGCC

Downstream 100 bases:

>100_bases
CGGCCGCGCAGGTGCACAGGTGCGCGGTGCCCGTTCAATAGTACGGGCCGTAGCGCATCTGCACGTCATCGACGTGGTTG
GTGCGCAAGTTGAAGAGGCA

Product: short-chain dehydrogenase/reductase SDR

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: ORFC [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTTDHSQPSQGESQKAAIVTGASRGIGAAISARLARDGFAVVINYANGADTAGDLRRKLEASGGRAMTFQADVSDASAVA
RMFDAAEATFDGVDVVVNNAGVMVLDTIAASDDAAFERVMNINLKGAFHTMREAARRLRHGGRIINLSSSVIGLRMERYG
IYAASKAAVEAMTAVLSKEMRGRAITVNAVAPGPTATDLFLDGKSPETIERLAKLNPLERLGTPEDIAAAVAFLASPDGA
WINGQVLRANGGMV

Sequences:

>Translated_254_residues
MTTDHSQPSQGESQKAAIVTGASRGIGAAISARLARDGFAVVINYANGADTAGDLRRKLEASGGRAMTFQADVSDASAVA
RMFDAAEATFDGVDVVVNNAGVMVLDTIAASDDAAFERVMNINLKGAFHTMREAARRLRHGGRIINLSSSVIGLRMERYG
IYAASKAAVEAMTAVLSKEMRGRAITVNAVAPGPTATDLFLDGKSPETIERLAKLNPLERLGTPEDIAAAVAFLASPDGA
WINGQVLRANGGMV
>Mature_253_residues
TTDHSQPSQGESQKAAIVTGASRGIGAAISARLARDGFAVVINYANGADTAGDLRRKLEASGGRAMTFQADVSDASAVAR
MFDAAEATFDGVDVVVNNAGVMVLDTIAASDDAAFERVMNINLKGAFHTMREAARRLRHGGRIINLSSSVIGLRMERYGI
YAASKAAVEAMTAVLSKEMRGRAITVNAVAPGPTATDLFLDGKSPETIERLAKLNPLERLGTPEDIAAAVAFLASPDGAW
INGQVLRANGGMV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=248, Percent_Identity=35.0806451612903, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI10190704, Length=243, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI40254992, Length=246, Percent_Identity=34.1463414634146, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI109715829, Length=243, Percent_Identity=30.4526748971193, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI5031737, Length=237, Percent_Identity=29.957805907173, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI32483357, Length=247, Percent_Identity=28.7449392712551, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI19923817, Length=250, Percent_Identity=27.6, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI126723750, Length=260, Percent_Identity=26.5384615384615, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI4504503, Length=196, Percent_Identity=31.1224489795918, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI40254986, Length=185, Percent_Identity=27.027027027027, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI59889578, Length=208, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4758504, Length=255, Percent_Identity=30.1960784313725, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI7705925, Length=240, Percent_Identity=28.3333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI7657478, Length=204, Percent_Identity=30.3921568627451, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI209862901, Length=204, Percent_Identity=26.9607843137255, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI19923983, Length=204, Percent_Identity=26.9607843137255, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4503817, Length=222, Percent_Identity=26.1261261261261, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI2367175, Length=244, Percent_Identity=34.8360655737705, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI87082160, Length=243, Percent_Identity=32.0987654320988, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1787335, Length=242, Percent_Identity=37.1900826446281, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI2367365, Length=239, Percent_Identity=34.3096234309623, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789208, Length=249, Percent_Identity=31.3253012048193, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=32.1285140562249, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789378, Length=242, Percent_Identity=30.1652892561983, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788459, Length=247, Percent_Identity=31.5789473684211, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1790717, Length=243, Percent_Identity=27.9835390946502, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=30.7086614173228, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789057, Length=257, Percent_Identity=26.8482490272374, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1786701, Length=191, Percent_Identity=34.0314136125654, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786812, Length=256, Percent_Identity=28.515625, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787820, Length=231, Percent_Identity=26.4069264069264, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17555706, Length=249, Percent_Identity=32.5301204819277, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17536651, Length=247, Percent_Identity=31.1740890688259, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17536025, Length=247, Percent_Identity=30.3643724696356, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17560150, Length=256, Percent_Identity=29.296875, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25147288, Length=245, Percent_Identity=30.2040816326531, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71994604, Length=245, Percent_Identity=29.3877551020408, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17561272, Length=212, Percent_Identity=32.5471698113208, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560676, Length=252, Percent_Identity=30.1587301587302, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17562990, Length=256, Percent_Identity=28.90625, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17531453, Length=258, Percent_Identity=29.0697674418605, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17559104, Length=258, Percent_Identity=28.2945736434109, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17562906, Length=258, Percent_Identity=28.6821705426357, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115534694, Length=245, Percent_Identity=28.1632653061224, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17563726, Length=263, Percent_Identity=28.1368821292776, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=28.5171102661597, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17561402, Length=255, Percent_Identity=28.2352941176471, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994600, Length=246, Percent_Identity=26.4227642276423, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17562910, Length=258, Percent_Identity=26.3565891472868, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17565030, Length=260, Percent_Identity=28.4615384615385, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=267, Percent_Identity=26.5917602996255, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17538480, Length=259, Percent_Identity=29.7297297297297, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=258, Percent_Identity=28.6821705426357, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17562908, Length=259, Percent_Identity=27.7992277992278, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17560332, Length=260, Percent_Identity=27.3076923076923, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71982365, Length=189, Percent_Identity=25.9259259259259, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=258, Percent_Identity=26.7441860465116, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=262, Percent_Identity=29.3893129770992, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=260, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558628, Length=189, Percent_Identity=28.042328042328, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17555612, Length=207, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=256, Percent_Identity=27.734375, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71997099, Length=203, Percent_Identity=26.6009852216749, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25152911, Length=194, Percent_Identity=30.4123711340206, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=268, Percent_Identity=25.7462686567164, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322226, Length=251, Percent_Identity=28.6852589641434, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324126, Length=187, Percent_Identity=31.0160427807487, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322067, Length=210, Percent_Identity=30.4761904761905, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=188, Percent_Identity=27.6595744680851, Blast_Score=62, Evalue=8e-11,
Organism=Drosophila melanogaster, GI28571526, Length=248, Percent_Identity=35.0806451612903, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=34.9206349206349, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24644339, Length=246, Percent_Identity=30.4878048780488, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI23397609, Length=251, Percent_Identity=33.4661354581673, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357041, Length=247, Percent_Identity=35.2226720647773, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=31.4516129032258, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI116007234, Length=237, Percent_Identity=29.957805907173, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI116007236, Length=255, Percent_Identity=27.4509803921569, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24643142, Length=239, Percent_Identity=30.1255230125523, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24644337, Length=167, Percent_Identity=35.9281437125749, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24641232, Length=252, Percent_Identity=28.1746031746032, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648693, Length=250, Percent_Identity=30, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355889, Length=250, Percent_Identity=30, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21357673, Length=187, Percent_Identity=29.9465240641711, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571387, Length=250, Percent_Identity=29.6, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24648944, Length=245, Percent_Identity=27.3469387755102, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24663911, Length=254, Percent_Identity=27.5590551181102, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI116007232, Length=179, Percent_Identity=31.2849162011173, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI116007238, Length=178, Percent_Identity=30.3370786516854, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364494, Length=260, Percent_Identity=30, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24641388, Length=252, Percent_Identity=29.7619047619048, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17737361, Length=256, Percent_Identity=28.90625, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24665243, Length=233, Percent_Identity=29.1845493562232, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221331218, Length=223, Percent_Identity=30.0448430493274, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665247, Length=234, Percent_Identity=29.0598290598291, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 26520; Mature: 26389

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDHSQPSQGESQKAAIVTGASRGIGAAISARLARDGFAVVINYANGADTAGDLRRKLE
CCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHH
ASGGRAMTFQADVSDASAVARMFDAAEATFDGVDVVVNNAGVMVLDTIAASDDAAFERVM
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCHHHHHHHH
NINLKGAFHTMREAARRLRHGGRIINLSSSVIGLRMERYGIYAASKAAVEAMTAVLSKEM
HCCCCHHHHHHHHHHHHHHHCCEEEEECCHHHEEEHHHHCEEHHHHHHHHHHHHHHHHHH
RGRAITVNAVAPGPTATDLFLDGKSPETIERLAKLNPLERLGTPEDIAAAVAFLASPDGA
CCCEEEEEEECCCCCCEEEEECCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCC
WINGQVLRANGGMV
EECCEEEECCCCCC
>Mature Secondary Structure 
TTDHSQPSQGESQKAAIVTGASRGIGAAISARLARDGFAVVINYANGADTAGDLRRKLE
CCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHH
ASGGRAMTFQADVSDASAVARMFDAAEATFDGVDVVVNNAGVMVLDTIAASDDAAFERVM
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCHHHHHHHH
NINLKGAFHTMREAARRLRHGGRIINLSSSVIGLRMERYGIYAASKAAVEAMTAVLSKEM
HCCCCHHHHHHHHHHHHHHHCCEEEEECCHHHEEEHHHHCEEHHHHHHHHHHHHHHHHHH
RGRAITVNAVAPGPTATDLFLDGKSPETIERLAKLNPLERLGTPEDIAAAVAFLASPDGA
CCCEEEEEEECCCCCCEEEEECCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCC
WINGQVLRANGGMV
EECCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]