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The map label for this gene is 94313444

Identifier: 94313444

GI number: 94313444

Start: 1123601

End: 1124959

Strand: Reverse

Name: 94313444

Synonym: Rmet_4519

Alternate gene names: NA

Gene position: 1124959-1123601 (Counterclockwise)

Preceding gene: 94313445

Following gene: 94313442

Centisome position: 43.6

GC content: 63.43

Gene sequence:

>1359_bases
ATGCACATCCAGTTCCTTGGGGCAACGGACACCGTGACCGGTTCGCGCTTTCTGATCGACACCGGAGAAGCGCGGGTGCT
GATCGACTGCGGACTGTTTCAGGGCTACAAGGCCCTGCGGCTACGTAACTGGGATCGCTTTCCGATCAGCCCGGAAAGTC
TCGATGCCGTGGTGTTGACACATGCGCATATCGACCACAGCGGCTATCTGCCCCTGTTGGTGAAACGCGGTTACAAGGGG
CGCGTGCACTGTACGACGGGAACCGCGGAGCTTTGCGGCCTGCTGCTGCCGGACAGCGCCCATCTGGCGGAAGAAGACGC
CAACTATGCCAACCGCAAGGGGTATTCGCGTCATCAACCGGCGCTGCCGCTCTATACCGTGGCGGATGCCATGCATGCGC
TGGAACATCTGAAGCCCGCGCCTTACAGCAAGCGCGTGACAATCGCACGCGGTGTGGAAGCGGAGTTTCATCGCGCGGGC
CATATTCTTGGCTCGTCCAGCGTGACGATCCATGCCAAGGGCCGGCGCATCCTGTTCTCCGGCGACCTCGGCCCGCAGGA
CGACATGCTGATGCGGCCGCCGGAGCCGCCCACCGCGGCGGATTACCTCATCGTGGAATCGACTTACGGTGACCGCCTGC
ACCCGGAGGGCGATCCCATCGAGCTGCTGGCCGATATCATCCGCAAGACCGTTGCCCGGGGCGGCAGCGTGATCATTCCC
GTCTTTGCGGTTGGGCGCGCCCAGTTGCTGCTGTATTGCCTCTACCGGCTGCGCCAGCGACGGCGCTTTCCCGATGTGCC
CATCTATCTGAACAGCCCGATGGCCATTGACGCCACGCGGATTCTCACCGAGCACACGGACGAACTGCGCATCGACCCCG
AAACGTGCGCGGCTGCTTGTTCGGTGGCGATTCCGACCCGGAGCATGGAGGAGTCGATCCGCATCAACCGGGTGGATCAG
CCGAAAATCATCCTGGCCGGTAGCGGAATGTTGACCGGCGGGCGCGTGGTGCATCACCTTGAATCGTTCGGGCAGGACCC
GCGCAGCACTTTTCTGCTGGTGGGGTTCCAGGCGGCGGGAACGCGGGGGGCGACGCTGGCGTCCGGTCAGCGCACATTGC
GTATGCACGGGCGCGAAGTCGAACTGCGTGCTTCAGTCGAACAGATTCGCCATTTCTCGGCACATGCCGATGCGGATGGG
CTGATGGACTGGATGAGTGCCATCGGGACGCCGCCACGGCAGACGTTCATCGTCCACGGCGAGCCCGGTGCCGCCGATGC
GCTACGCCAGCGCATCGAGCATTCGCTGCATTGGGATGTCACGATGCCCGAGTACCGGGGTATCTATCCGCTCGACTAA

Upstream 100 bases:

>100_bases
CGTGGTACGTACTGTTTTGCCATCGTTTCAAAGCCAGTGAGCGGCTACGGCAATACTGACAACACCGCTACTGACGACGC
CAACACCAACATCACAGACC

Downstream 100 bases:

>100_bases
GCGCCCGACTAAGCGCTCGACTAAGCGCTCGACTAAGCGCTCGACTGAGCGCCCGAGCAAGGGGTGTCGGCGCGGCTAGT
AGGAAAAGAGGGTGGGAATC

Product: exonuclease/RNA metabolizing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MHIQFLGATDTVTGSRFLIDTGEARVLIDCGLFQGYKALRLRNWDRFPISPESLDAVVLTHAHIDHSGYLPLLVKRGYKG
RVHCTTGTAELCGLLLPDSAHLAEEDANYANRKGYSRHQPALPLYTVADAMHALEHLKPAPYSKRVTIARGVEAEFHRAG
HILGSSSVTIHAKGRRILFSGDLGPQDDMLMRPPEPPTAADYLIVESTYGDRLHPEGDPIELLADIIRKTVARGGSVIIP
VFAVGRAQLLLYCLYRLRQRRRFPDVPIYLNSPMAIDATRILTEHTDELRIDPETCAAACSVAIPTRSMEESIRINRVDQ
PKIILAGSGMLTGGRVVHHLESFGQDPRSTFLLVGFQAAGTRGATLASGQRTLRMHGREVELRASVEQIRHFSAHADADG
LMDWMSAIGTPPRQTFIVHGEPGAADALRQRIEHSLHWDVTMPEYRGIYPLD

Sequences:

>Translated_452_residues
MHIQFLGATDTVTGSRFLIDTGEARVLIDCGLFQGYKALRLRNWDRFPISPESLDAVVLTHAHIDHSGYLPLLVKRGYKG
RVHCTTGTAELCGLLLPDSAHLAEEDANYANRKGYSRHQPALPLYTVADAMHALEHLKPAPYSKRVTIARGVEAEFHRAG
HILGSSSVTIHAKGRRILFSGDLGPQDDMLMRPPEPPTAADYLIVESTYGDRLHPEGDPIELLADIIRKTVARGGSVIIP
VFAVGRAQLLLYCLYRLRQRRRFPDVPIYLNSPMAIDATRILTEHTDELRIDPETCAAACSVAIPTRSMEESIRINRVDQ
PKIILAGSGMLTGGRVVHHLESFGQDPRSTFLLVGFQAAGTRGATLASGQRTLRMHGREVELRASVEQIRHFSAHADADG
LMDWMSAIGTPPRQTFIVHGEPGAADALRQRIEHSLHWDVTMPEYRGIYPLD
>Mature_452_residues
MHIQFLGATDTVTGSRFLIDTGEARVLIDCGLFQGYKALRLRNWDRFPISPESLDAVVLTHAHIDHSGYLPLLVKRGYKG
RVHCTTGTAELCGLLLPDSAHLAEEDANYANRKGYSRHQPALPLYTVADAMHALEHLKPAPYSKRVTIARGVEAEFHRAG
HILGSSSVTIHAKGRRILFSGDLGPQDDMLMRPPEPPTAADYLIVESTYGDRLHPEGDPIELLADIIRKTVARGGSVIIP
VFAVGRAQLLLYCLYRLRQRRRFPDVPIYLNSPMAIDATRILTEHTDELRIDPETCAAACSVAIPTRSMEESIRINRVDQ
PKIILAGSGMLTGGRVVHHLESFGQDPRSTFLLVGFQAAGTRGATLASGQRTLRMHGREVELRASVEQIRHFSAHADADG
LMDWMSAIGTPPRQTFIVHGEPGAADALRQRIEHSLHWDVTMPEYRGIYPLD

Specific function: Has endoribonuclease activity towards 23S and 16S rRNA (in vitro) [H]

COG id: COG1236

COG function: function code J; Predicted exonuclease of the beta-lactamase fold involved in RNA processing

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family [H]

Homologues:

Organism=Homo sapiens, GI33300633, Length=466, Percent_Identity=29.6137339055794, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI7706427, Length=435, Percent_Identity=27.5862068965517, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32564696, Length=463, Percent_Identity=27.8617710583153, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32566029, Length=447, Percent_Identity=26.6219239373602, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323307, Length=442, Percent_Identity=26.6968325791855, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24648013, Length=452, Percent_Identity=28.0973451327434, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21358523, Length=470, Percent_Identity=27.6595744680851, Blast_Score=148, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022712
- InterPro:   IPR001279
- InterPro:   IPR011108 [H]

Pfam domain/function: PF10996 Beta-Casp; PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 50025; Mature: 50025

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIQFLGATDTVTGSRFLIDTGEARVLIDCGLFQGYKALRLRNWDRFPISPESLDAVVLT
CEEEEECCCCCCCCCEEEEECCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCEEEEE
HAHIDHSGYLPLLVKRGYKGRVHCTTGTAELCGLLLPDSAHLAEEDANYANRKGYSRHQP
EEEECCCCCCCCEEECCCCCEEEEECCHHHHHHEECCCCHHHHHHCCCHHHCCCCCCCCC
ALPLYTVADAMHALEHLKPAPYSKRVTIARGVEAEFHRAGHILGSSSVTIHAKGRRILFS
CCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHCEEECCCCEEEEECCCEEEEE
GDLGPQDDMLMRPPEPPTAADYLIVESTYGDRLHPEGDPIELLADIIRKTVARGGSVIIP
CCCCCCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
VFAVGRAQLLLYCLYRLRQRRRFPDVPIYLNSPMAIDATRILTEHTDELRIDPETCAAAC
EEHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEECHHHHHHCCCCCEEECHHHHHHHH
SVAIPTRSMEESIRINRVDQPKIILAGSGMLTGGRVVHHLESFGQDPRSTFLLVGFQAAG
EEECCCCCHHHCCEECCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCC
TRGATLASGQRTLRMHGREVELRASVEQIRHFSAHADADGLMDWMSAIGTPPRQTFIVHG
CCCCEECCCCEEEEECCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEC
EPGAADALRQRIEHSLHWDVTMPEYRGIYPLD
CCCHHHHHHHHHHHCCEECEECCCCCCCCCCC
>Mature Secondary Structure
MHIQFLGATDTVTGSRFLIDTGEARVLIDCGLFQGYKALRLRNWDRFPISPESLDAVVLT
CEEEEECCCCCCCCCEEEEECCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCEEEEE
HAHIDHSGYLPLLVKRGYKGRVHCTTGTAELCGLLLPDSAHLAEEDANYANRKGYSRHQP
EEEECCCCCCCCEEECCCCCEEEEECCHHHHHHEECCCCHHHHHHCCCHHHCCCCCCCCC
ALPLYTVADAMHALEHLKPAPYSKRVTIARGVEAEFHRAGHILGSSSVTIHAKGRRILFS
CCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHCEEECCCCEEEEECCCEEEEE
GDLGPQDDMLMRPPEPPTAADYLIVESTYGDRLHPEGDPIELLADIIRKTVARGGSVIIP
CCCCCCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
VFAVGRAQLLLYCLYRLRQRRRFPDVPIYLNSPMAIDATRILTEHTDELRIDPETCAAAC
EEHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEECHHHHHHCCCCCEEECHHHHHHHH
SVAIPTRSMEESIRINRVDQPKIILAGSGMLTGGRVVHHLESFGQDPRSTFLLVGFQAAG
EEECCCCCHHHCCEECCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCC
TRGATLASGQRTLRMHGREVELRASVEQIRHFSAHADADGLMDWMSAIGTPPRQTFIVHG
CCCCEECCCCEEEEECCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEC
EPGAADALRQRIEHSLHWDVTMPEYRGIYPLD
CCCHHHHHHHHHHHCCEECEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA