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The map label for this gene is amaB-3 [H]

Identifier: 94313405

GI number: 94313405

Start: 1079810

End: 1081072

Strand: Direct

Name: amaB-3 [H]

Synonym: Rmet_4480

Alternate gene names: 94313405

Gene position: 1079810-1081072 (Clockwise)

Preceding gene: 94313404

Following gene: 94313406

Centisome position: 41.85

GC content: 63.97

Gene sequence:

>1263_bases
ATGAGCAACACGCAAGCATCTGCCGGCATCGGACATGATGTGCGCATCAACGGCTCCCGCCTGTGGGACACGCTGATGCG
CCTTGCCGAGATTGGCGCGACCCCCAAAGGGGGCGTCTGCCGTCTTGCATTGACTGATCTGGACCGCCAGGGCAGGGATT
TCTTCGTGAAGGAGGCCGAAGCGGCCGGCTGCACGATCCGCGTCGACGCCATCGGCAATATCTTTGCGCGCAGGCGTGGC
CGCAACGACAGCCTGCCGGCCGTCATGACCGGAAGCCACATCGACACCCAGCCGACAGGCGGCAAGTTCGACGGGAACTA
CGGCGTGTTCGCCGGCATCGAGGTCCTCAGGACGCTCAACGACCGCGACATTGTCACGGAGGCCCCGCTCGAAGTGGCAG
TCTGGACGAATGAAGAGGGATCTCGGTTCGTGCCCGTGATGATGGGATCTGGCGCGTTCATCGGAGAATTCGTCCTCAAG
GACTTGCTGGTGCAGACCGACCGCGAGGGCATCTCCGTCAAGGATGCCCTGGAGGCCATTAGCTATGCGGGCTCGGCACC
GATTGGCGAACCCCGGCCCGGCGCGTACTTCGAGGCTCATATCGAACAGGGGCCGGTGCTGGAGGCACACGACACCGTGA
TCGGCGTCGTGACTGGCGCACTGGGGCAGCGCTGGTACGACGTCACCATCACGGGCATGGAAGCCCACGCGGGACCGACG
CCGATGGCGCTACGCAAGGACGCGCTGCTTGCCGCTTCGGAGTTGGTCGGCATCGTCAACCGTATCGCGCTGGACCATCC
GCCGCACGGGCGCGGTACCGTAGGCTGCCTGACGGTCCATCCCGACTCCCGCAACGTGATTCCCGGACGCGTCAACATGA
CCGTCGATCTTCGCGCATCGGACGACGATCTATTGACCTCGATGCACGACACGTTCCTGGCCCAGACACGCGAACTGGCG
GTACGTAACGGTGTCTCGATCGATGTCAAGCAGGTTGTCTACTTCCCGCCGCAGCCATTCGACGAGCGGCTGATCCGCGC
CATTCGCGCGGGCGCCGGACGGATCGGCCATTCGGAGATGGACGTCATCAGCGGCGCTGGCCACGACGCCGTCTATCTCG
CGCGCGTGGCCCCGACCGCTATGATTTTTGTGCCTTGCAAGGACGGCATCAGCCACAACGAGATCGAGGATGCGAAACCA
GAGCATCTGGAGGCGGGGGCCAACGTGCTGCTTCATGCCATGCTCGATGCGGCCAATCGGTAA

Upstream 100 bases:

>100_bases
ACACCGGCGGCAACGAGGAGTTCCGCCGCAACGACGCGGTCAAGGACAAGTACCTCGGCGTCGGCCTTACCGTCTGATCC
GCCATCGCACTGGAGAAAGC

Downstream 100 bases:

>100_bases
TCGTCGACGTCATTCCGATCATTCAGGACACCCATGAAGATTCTGATCGCCAGACTCAATCACGAAACAAACACGTACTC
GCCGGTGGCAACACCACTTG

Product: N-carbamoyl-L-amino-acid hydrolase

Products: NA

Alternate protein names: L-carbamoylase [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSNTQASAGIGHDVRINGSRLWDTLMRLAEIGATPKGGVCRLALTDLDRQGRDFFVKEAEAAGCTIRVDAIGNIFARRRG
RNDSLPAVMTGSHIDTQPTGGKFDGNYGVFAGIEVLRTLNDRDIVTEAPLEVAVWTNEEGSRFVPVMMGSGAFIGEFVLK
DLLVQTDREGISVKDALEAISYAGSAPIGEPRPGAYFEAHIEQGPVLEAHDTVIGVVTGALGQRWYDVTITGMEAHAGPT
PMALRKDALLAASELVGIVNRIALDHPPHGRGTVGCLTVHPDSRNVIPGRVNMTVDLRASDDDLLTSMHDTFLAQTRELA
VRNGVSIDVKQVVYFPPQPFDERLIRAIRAGAGRIGHSEMDVISGAGHDAVYLARVAPTAMIFVPCKDGISHNEIEDAKP
EHLEAGANVLLHAMLDAANR

Sequences:

>Translated_420_residues
MSNTQASAGIGHDVRINGSRLWDTLMRLAEIGATPKGGVCRLALTDLDRQGRDFFVKEAEAAGCTIRVDAIGNIFARRRG
RNDSLPAVMTGSHIDTQPTGGKFDGNYGVFAGIEVLRTLNDRDIVTEAPLEVAVWTNEEGSRFVPVMMGSGAFIGEFVLK
DLLVQTDREGISVKDALEAISYAGSAPIGEPRPGAYFEAHIEQGPVLEAHDTVIGVVTGALGQRWYDVTITGMEAHAGPT
PMALRKDALLAASELVGIVNRIALDHPPHGRGTVGCLTVHPDSRNVIPGRVNMTVDLRASDDDLLTSMHDTFLAQTRELA
VRNGVSIDVKQVVYFPPQPFDERLIRAIRAGAGRIGHSEMDVISGAGHDAVYLARVAPTAMIFVPCKDGISHNEIEDAKP
EHLEAGANVLLHAMLDAANR
>Mature_419_residues
SNTQASAGIGHDVRINGSRLWDTLMRLAEIGATPKGGVCRLALTDLDRQGRDFFVKEAEAAGCTIRVDAIGNIFARRRGR
NDSLPAVMTGSHIDTQPTGGKFDGNYGVFAGIEVLRTLNDRDIVTEAPLEVAVWTNEEGSRFVPVMMGSGAFIGEFVLKD
LLVQTDREGISVKDALEAISYAGSAPIGEPRPGAYFEAHIEQGPVLEAHDTVIGVVTGALGQRWYDVTITGMEAHAGPTP
MALRKDALLAASELVGIVNRIALDHPPHGRGTVGCLTVHPDSRNVIPGRVNMTVDLRASDDDLLTSMHDTFLAQTRELAV
RNGVSIDVKQVVYFPPQPFDERLIRAIRAGAGRIGHSEMDVISGAGHDAVYLARVAPTAMIFVPCKDGISHNEIEDAKPE
HLEAGANVLLHAMLDAANR

Specific function: Involved In The Anaerobic Utilization Of Allantoin. Converts Allantoate To (S)-Ureidoglycolate And Ammonia. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI1786726, Length=391, Percent_Identity=33.5038363171356, Blast_Score=187, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010158
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.87 [H]

Molecular weight: Translated: 45074; Mature: 44943

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTQASAGIGHDVRINGSRLWDTLMRLAEIGATPKGGVCRLALTDLDRQGRDFFVKEAE
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEEEECC
AAGCTIRVDAIGNIFARRRGRNDSLPAVMTGSHIDTQPTGGKFDGNYGVFAGIEVLRTLN
CCCCEEEEECHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
DRDIVTEAPLEVAVWTNEEGSRFVPVMMGSGAFIGEFVLKDLLVQTDREGISVKDALEAI
CCCCEECCCEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
SYAGSAPIGEPRPGAYFEAHIEQGPVLEAHDTVIGVVTGALGQRWYDVTITGMEAHAGPT
HHCCCCCCCCCCCCCEEEEECCCCCEEECCCCEEEEHHHHHCCEEEEEEEEEEECCCCCC
PMALRKDALLAASELVGIVNRIALDHPPHGRGTVGCLTVHPDSRNVIPGRVNMTVDLRAS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEECC
DDDLLTSMHDTFLAQTRELAVRNGVSIDVKQVVYFPPQPFDERLIRAIRAGAGRIGHSEM
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHH
DVISGAGHDAVYLARVAPTAMIFVPCKDGISHNEIEDAKPEHLEAGANVLLHAMLDAANR
HHHCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNTQASAGIGHDVRINGSRLWDTLMRLAEIGATPKGGVCRLALTDLDRQGRDFFVKEAE
CCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEEEECC
AAGCTIRVDAIGNIFARRRGRNDSLPAVMTGSHIDTQPTGGKFDGNYGVFAGIEVLRTLN
CCCCEEEEECHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
DRDIVTEAPLEVAVWTNEEGSRFVPVMMGSGAFIGEFVLKDLLVQTDREGISVKDALEAI
CCCCEECCCEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
SYAGSAPIGEPRPGAYFEAHIEQGPVLEAHDTVIGVVTGALGQRWYDVTITGMEAHAGPT
HHCCCCCCCCCCCCCEEEEECCCCCEEECCCCEEEEHHHHHCCEEEEEEEEEEECCCCCC
PMALRKDALLAASELVGIVNRIALDHPPHGRGTVGCLTVHPDSRNVIPGRVNMTVDLRAS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEECC
DDDLLTSMHDTFLAQTRELAVRNGVSIDVKQVVYFPPQPFDERLIRAIRAGAGRIGHSEM
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHH
DVISGAGHDAVYLARVAPTAMIFVPCKDGISHNEIEDAKPEHLEAGANVLLHAMLDAANR
HHHCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9023955; 8285691 [H]