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The map label for this gene is garR [C]

Identifier: 94313384

GI number: 94313384

Start: 1055830

End: 1056705

Strand: Reverse

Name: garR [C]

Synonym: Rmet_4459

Alternate gene names: 94313384

Gene position: 1056705-1055830 (Counterclockwise)

Preceding gene: 94313386

Following gene: 94313380

Centisome position: 40.96

GC content: 65.98

Gene sequence:

>876_bases
ATGCGGGTTGCATTCCTCGGGCTCGGCGTCATGGGCTACCCCATGGCCGGCCATCTGGCCGCCAAGGGCCATGACGTCAC
CGTTTACAACCGTACGGCAGCCAAGGCGGAGCGCTGGGTCCAGGCCTTTGGCAAGCACGGTGGCAAACAGGCAGCGACGC
CGGCGCTGGCCGCAGTCGACTGTGACATCGTTTGTGCCTGTGTTGGCAATGACGATGACCTGCGCGCGGTCATGACGGGG
CCAGATGGCGCGTTCCAGCACGCGGCGCCAGGCACTATCTTCGTCGACCACACCACCGCCAGCGCCAGCGTAGCGCGCGA
ACTGCACGAGGCCGCACGCGAGCGCGGCTGCCATTTCATCGATGCACCCGTTTCGGGCGGACAGGCTGGCGCCGAACAGG
GCATCCTGACTATCATGTGCGGGGGCGACCCGGAAGCGTTCCAGCGCGCCGAGCCCGTCATCGCCGCCTATGCCCGCGCC
GTCACCCGCATCGGAGAGAGCGGGGCCGGCCAGTTGGCCAAGATGGTCAACCAGATCAGCGTCGCCGGCCTGCTGCAGGC
GCTTTCCGAAGCCATCGCGTTTGGCGAGCACGCCGGGCTGGACATGCGCGTGGTGCTCGACGTCATCAGCAAGGGCGCGG
CGTCATCGTGGCAGATGGAAAACCGTGGCCCGACGATGCTCCAGAGCAAATTCGACTTCGGTTTCGCCGTGGACTGGATG
CGCAAGGACCTGGGCCTGTGCCTGGACGAAGCCCGCCGCAATGGCGCCACGCTGCCGGTCACATCGCTAGTCGACCAGTT
CTATGCAGACCTGCAACAAATGGGAAGCTCGCGAGACGACACCTCGTCGCTGATCAAGCGCTTGCGTCGCTCGTGA

Upstream 100 bases:

>100_bases
ATGGGGCGATGACTTCGAGGTGGCGGCATTCTGACATCGACCGCCACTGCCCGCAAACGACCTATACTCTTGCCTCTCTT
CGAAGTCACGGAGCAAAGTC

Downstream 100 bases:

>100_bases
CCCGTGCTCCGGCGCGCGGTGCCTGCCGTATCGAGACAGGAATAGACTGACCGTCCGACCATCCGACCGTCCGACAAACA
CCTACACCGCTTTCAGCACG

Product: NADP oxidoreductase, coenzyme F420-dependent:6-phosphogluconate dehydrogenase, NAD-binding

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MRVAFLGLGVMGYPMAGHLAAKGHDVTVYNRTAAKAERWVQAFGKHGGKQAATPALAAVDCDIVCACVGNDDDLRAVMTG
PDGAFQHAAPGTIFVDHTTASASVARELHEAARERGCHFIDAPVSGGQAGAEQGILTIMCGGDPEAFQRAEPVIAAYARA
VTRIGESGAGQLAKMVNQISVAGLLQALSEAIAFGEHAGLDMRVVLDVISKGAASSWQMENRGPTMLQSKFDFGFAVDWM
RKDLGLCLDEARRNGATLPVTSLVDQFYADLQQMGSSRDDTSSLIKRLRRS

Sequences:

>Translated_291_residues
MRVAFLGLGVMGYPMAGHLAAKGHDVTVYNRTAAKAERWVQAFGKHGGKQAATPALAAVDCDIVCACVGNDDDLRAVMTG
PDGAFQHAAPGTIFVDHTTASASVARELHEAARERGCHFIDAPVSGGQAGAEQGILTIMCGGDPEAFQRAEPVIAAYARA
VTRIGESGAGQLAKMVNQISVAGLLQALSEAIAFGEHAGLDMRVVLDVISKGAASSWQMENRGPTMLQSKFDFGFAVDWM
RKDLGLCLDEARRNGATLPVTSLVDQFYADLQQMGSSRDDTSSLIKRLRRS
>Mature_291_residues
MRVAFLGLGVMGYPMAGHLAAKGHDVTVYNRTAAKAERWVQAFGKHGGKQAATPALAAVDCDIVCACVGNDDDLRAVMTG
PDGAFQHAAPGTIFVDHTTASASVARELHEAARERGCHFIDAPVSGGQAGAEQGILTIMCGGDPEAFQRAEPVIAAYARA
VTRIGESGAGQLAKMVNQISVAGLLQALSEAIAFGEHAGLDMRVVLDVISKGAASSWQMENRGPTMLQSKFDFGFAVDWM
RKDLGLCLDEARRNGATLPVTSLVDQFYADLQQMGSSRDDTSSLIKRLRRS

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=286, Percent_Identity=28.3216783216783, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI23308751, Length=295, Percent_Identity=28.135593220339, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI145693186, Length=284, Percent_Identity=33.4507042253521, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=32.5259515570934, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790315, Length=292, Percent_Identity=29.7945205479452, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789092, Length=227, Percent_Identity=35.2422907488987, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788341, Length=212, Percent_Identity=29.2452830188679, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17557316, Length=272, Percent_Identity=30.8823529411765, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24655230, Length=299, Percent_Identity=29.4314381270903, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI19922568, Length=299, Percent_Identity=29.4314381270903, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI28574115, Length=286, Percent_Identity=24.4755244755245, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30808; Mature: 30808

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAFLGLGVMGYPMAGHLAAKGHDVTVYNRTAAKAERWVQAFGKHGGKQAATPALAAVD
CEEEEEECCHHCCCCCCHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHEEC
CDIVCACVGNDDDLRAVMTGPDGAFQHAAPGTIFVDHTTASASVARELHEAARERGCHFI
CEEEEEEECCCCCCEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEE
DAPVSGGQAGAEQGILTIMCGGDPEAFQRAEPVIAAYARAVTRIGESGAGQLAKMVNQIS
ECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VAGLLQALSEAIAFGEHAGLDMRVVLDVISKGAASSWQMENRGPTMLQSKFDFGFAVDWM
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCHHHHHH
RKDLGLCLDEARRNGATLPVTSLVDQFYADLQQMGSSRDDTSSLIKRLRRS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRVAFLGLGVMGYPMAGHLAAKGHDVTVYNRTAAKAERWVQAFGKHGGKQAATPALAAVD
CEEEEEECCHHCCCCCCHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHEEC
CDIVCACVGNDDDLRAVMTGPDGAFQHAAPGTIFVDHTTASASVARELHEAARERGCHFI
CEEEEEEECCCCCCEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEE
DAPVSGGQAGAEQGILTIMCGGDPEAFQRAEPVIAAYARAVTRIGESGAGQLAKMVNQIS
ECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VAGLLQALSEAIAFGEHAGLDMRVVLDVISKGAASSWQMENRGPTMLQSKFDFGFAVDWM
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCHHHHHH
RKDLGLCLDEARRNGATLPVTSLVDQFYADLQQMGSSRDDTSSLIKRLRRS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA