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The map label for this gene is potH [H]

Identifier: 94313323

GI number: 94313323

Start: 994541

End: 995557

Strand: Reverse

Name: potH [H]

Synonym: Rmet_4398

Alternate gene names: 94313323

Gene position: 995557-994541 (Counterclockwise)

Preceding gene: 94313324

Following gene: 94313322

Centisome position: 38.59

GC content: 65.09

Gene sequence:

>1017_bases
ATGATCACGCGCGCGGATCGGCTGGCCCGCTGGCTTGTCTGTGGCATCCCCATCGCCTTCCTGCTCGTATTCTTCGGCAT
TCCATCGGCCATCATGATCGTGGCGGCATTCCGGACACCGGCGGAGTCCGGCGGATTGCTGCCGCTGATCCCGCCTGACC
AGGGTCTGACCACCGCCGCGGCCATCACCATGATCAGGCACTGGCCGGACCTGTTCACGCTCGACAACCTCCGCGAGTTG
ATCCAGGAACCACTCTATGCCGGGCTGTTTCTCAAATCAGCCGGCTACGCCGCGCTGACCACCGCGATCTGCCTGGTGGC
TGCCTACCCGCTGGCATGGTGGATTGCCCGCAGCGACAAGCGACGGCGCGACCTGCTGCTGATCCTTGTGATCCTGCCGT
TCTGGTCGAACTTCCTGATCCGGATCTACGCGTGGATGATCATCCTGAGTCCACGCGGATACCTGATGCATGGCGTCAAC
AGCTTCATCGGCTGGATGGGCTTCGCCCCGGTCGAAGCGCTCTACACCCCCGCTGCCGTCATCCTCGTGCTGGTCTACGT
GCACCTGCCGTTCTTCATCCTGCCCCTCTATGCCAGCCTGGAAAAGCAGGACCCCCTGCTGCTCGATGCCGCGCGCGACC
TGGGCGCGAATGGCTGGCATTGCTTCTGGCAGGTGATCTGGCCGCTTTCGCTGCCGGGCGTCTACGCGGGCATCGCGCTC
GTCTTCACACCGTGTCTGGGGATCTTTGCCGTGCCCGAACTGGTGGGCGGCACGCGATCGATCCTGATCGGCAACCTGAT
CAAGCAGCAGTTCCTCGATAGCCGAGACTGGCCGCTGGGGTCGGCGCTATCGCTGGCGCTGGTGGGCATCGTGCTGGCGT
TGATCGGACTGGCAACGCTGGCGGCACGCGTGAAGGGCACGGCGCGTGCCGCCTCGCAGCGGGGTACGCACAAGTCCGCC
CATCGTCCAGCGCAACCCGCGCCGGCCGCCGTGGCCACTGGCAGGAGATCGTCATGA

Upstream 100 bases:

>100_bases
GCCGGTGACCGACGGGCAGTTTACGCGGGGCGATCGCGTCGAGCTCGACTGGCCCGGCAACGCCGGCGTGTTTCTAAGCC
GGTGAGGCAAAGAGACGGCC

Downstream 100 bases:

>100_bases
ATCGCCCCCGTTGGCCTGTGTGGGTGGCCTGCCTGGGCTGCGCGGTCTATGCGTTCCTGTACCTGCCGTTGCTGATCGTC
GTGGCCTACTCGTTCAATGA

Product: putrescine ABC transporter membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MITRADRLARWLVCGIPIAFLLVFFGIPSAIMIVAAFRTPAESGGLLPLIPPDQGLTTAAAITMIRHWPDLFTLDNLREL
IQEPLYAGLFLKSAGYAALTTAICLVAAYPLAWWIARSDKRRRDLLLILVILPFWSNFLIRIYAWMIILSPRGYLMHGVN
SFIGWMGFAPVEALYTPAAVILVLVYVHLPFFILPLYASLEKQDPLLLDAARDLGANGWHCFWQVIWPLSLPGVYAGIAL
VFTPCLGIFAVPELVGGTRSILIGNLIKQQFLDSRDWPLGSALSLALVGIVLALIGLATLAARVKGTARAASQRGTHKSA
HRPAQPAPAAVATGRRSS

Sequences:

>Translated_338_residues
MITRADRLARWLVCGIPIAFLLVFFGIPSAIMIVAAFRTPAESGGLLPLIPPDQGLTTAAAITMIRHWPDLFTLDNLREL
IQEPLYAGLFLKSAGYAALTTAICLVAAYPLAWWIARSDKRRRDLLLILVILPFWSNFLIRIYAWMIILSPRGYLMHGVN
SFIGWMGFAPVEALYTPAAVILVLVYVHLPFFILPLYASLEKQDPLLLDAARDLGANGWHCFWQVIWPLSLPGVYAGIAL
VFTPCLGIFAVPELVGGTRSILIGNLIKQQFLDSRDWPLGSALSLALVGIVLALIGLATLAARVKGTARAASQRGTHKSA
HRPAQPAPAAVATGRRSS
>Mature_338_residues
MITRADRLARWLVCGIPIAFLLVFFGIPSAIMIVAAFRTPAESGGLLPLIPPDQGLTTAAAITMIRHWPDLFTLDNLREL
IQEPLYAGLFLKSAGYAALTTAICLVAAYPLAWWIARSDKRRRDLLLILVILPFWSNFLIRIYAWMIILSPRGYLMHGVN
SFIGWMGFAPVEALYTPAAVILVLVYVHLPFFILPLYASLEKQDPLLLDAARDLGANGWHCFWQVIWPLSLPGVYAGIAL
VFTPCLGIFAVPELVGGTRSILIGNLIKQQFLDSRDWPLGSALSLALVGIVLALIGLATLAARVKGTARAASQRGTHKSA
HRPAQPAPAAVATGRRSS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=229, Percent_Identity=48.9082969432314, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1787080, Length=260, Percent_Identity=40.3846153846154, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787713, Length=259, Percent_Identity=33.2046332046332, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36883; Mature: 36883

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRADRLARWLVCGIPIAFLLVFFGIPSAIMIVAAFRTPAESGGLLPLIPPDQGLTTAA
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHH
AITMIRHWPDLFTLDNLRELIQEPLYAGLFLKSAGYAALTTAICLVAAYPLAWWIARSDK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
RRRDLLLILVILPFWSNFLIRIYAWMIILSPRGYLMHGVNSFIGWMGFAPVEALYTPAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ILVLVYVHLPFFILPLYASLEKQDPLLLDAARDLGANGWHCFWQVIWPLSLPGVYAGIAL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHH
VFTPCLGIFAVPELVGGTRSILIGNLIKQQFLDSRDWPLGSALSLALVGIVLALIGLATL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AARVKGTARAASQRGTHKSAHRPAQPAPAAVATGRRSS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MITRADRLARWLVCGIPIAFLLVFFGIPSAIMIVAAFRTPAESGGLLPLIPPDQGLTTAA
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHH
AITMIRHWPDLFTLDNLRELIQEPLYAGLFLKSAGYAALTTAICLVAAYPLAWWIARSDK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
RRRDLLLILVILPFWSNFLIRIYAWMIILSPRGYLMHGVNSFIGWMGFAPVEALYTPAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ILVLVYVHLPFFILPLYASLEKQDPLLLDAARDLGANGWHCFWQVIWPLSLPGVYAGIAL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHH
VFTPCLGIFAVPELVGGTRSILIGNLIKQQFLDSRDWPLGSALSLALVGIVLALIGLATL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AARVKGTARAASQRGTHKSAHRPAQPAPAAVATGRRSS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]