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The map label for this gene is potI [H]

Identifier: 94313322

GI number: 94313322

Start: 993762

End: 994544

Strand: Reverse

Name: potI [H]

Synonym: Rmet_4397

Alternate gene names: 94313322

Gene position: 994544-993762 (Counterclockwise)

Preceding gene: 94313323

Following gene: 94313321

Centisome position: 38.55

GC content: 64.62

Gene sequence:

>783_bases
ATGAATCGCCCCCGTTGGCCTGTGTGGGTGGCCTGCCTGGGCTGCGCGGTCTATGCGTTCCTGTACCTGCCGTTGCTGAT
CGTCGTGGCCTACTCGTTCAATGACTCACGCCTGAACGCGGAATGGGACGGCTTCACGCTGCACTGGTATGACGTGCTGG
CAAGCGATCCGGACATGCTGGGTGCGGCGTGGAACTCGTTGATGATCGGCGCGATCGCGGCAGGCACGTCATGCGTGCTC
GGCACGCTCGCCGCCATCGCGCTGCACCGCTACCGGCCGCCATTGCTGGCTACGCTGGTGCTCGCGCCGATCGCGATGCC
CGAGGTACTGATGGGGGTGTCGCTGCTGTTGTTCTTTGTTGCCGTGAATATCGATCTCGGCCTGACAGCCGTGGCGCTGT
CGCATATCACGTTCTGCGTGGGCTTCGTTGCCGTGGTGGTGCGTGCCCGGCTCGAAGGGCTCGACGAGAGCCTGATCGAC
GCCGCCCGCGATCTCGGCGCCTCACCGCTTCAGGCCGGGTGGCATGTCATGCTGCCGCTGATGCTGCCGGGCGTCCTCGC
GGGCGCGTTGCTGGCCTTCACCCTGTCGATGGACGACTTCGTCGTGACCTTCTTCACGGCGGGGCCCGGCCAACCGACAC
TGCCGCTGCAGATTTACTCGATGATCCGGATCGCGGTCACGCCGGAAGTGAATGCGATCTCGTCCATCCTCATGATGCTG
ACACTGGCCGTGTCGCTCACGGCCTATCGCGTTTCGCGCGACGTCTGGAGGGCGCAACTATGA

Upstream 100 bases:

>100_bases
AGGGCACGGCGCGTGCCGCCTCGCAGCGGGGTACGCACAAGTCCGCCCATCGTCCAGCGCAACCCGCGCCGGCCGCCGTG
GCCACTGGCAGGAGATCGTC

Downstream 100 bases:

>100_bases
GTCATCGCATATGGACGAAGGTGCGCCATCTGCTGGTGAGCCTGACGCTGATGGGGTGCTTTGGCCTGGCCATGGCCACG
GAAGACCTGAACGTATTCAC

Product: binding-protein-dependent transport systems inner membrane component

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNRPRWPVWVACLGCAVYAFLYLPLLIVVAYSFNDSRLNAEWDGFTLHWYDVLASDPDMLGAAWNSLMIGAIAAGTSCVL
GTLAAIALHRYRPPLLATLVLAPIAMPEVLMGVSLLLFFVAVNIDLGLTAVALSHITFCVGFVAVVVRARLEGLDESLID
AARDLGASPLQAGWHVMLPLMLPGVLAGALLAFTLSMDDFVVTFFTAGPGQPTLPLQIYSMIRIAVTPEVNAISSILMML
TLAVSLTAYRVSRDVWRAQL

Sequences:

>Translated_260_residues
MNRPRWPVWVACLGCAVYAFLYLPLLIVVAYSFNDSRLNAEWDGFTLHWYDVLASDPDMLGAAWNSLMIGAIAAGTSCVL
GTLAAIALHRYRPPLLATLVLAPIAMPEVLMGVSLLLFFVAVNIDLGLTAVALSHITFCVGFVAVVVRARLEGLDESLID
AARDLGASPLQAGWHVMLPLMLPGVLAGALLAFTLSMDDFVVTFFTAGPGQPTLPLQIYSMIRIAVTPEVNAISSILMML
TLAVSLTAYRVSRDVWRAQL
>Mature_260_residues
MNRPRWPVWVACLGCAVYAFLYLPLLIVVAYSFNDSRLNAEWDGFTLHWYDVLASDPDMLGAAWNSLMIGAIAAGTSCVL
GTLAAIALHRYRPPLLATLVLAPIAMPEVLMGVSLLLFFVAVNIDLGLTAVALSHITFCVGFVAVVVRARLEGLDESLID
AARDLGASPLQAGWHVMLPLMLPGVLAGALLAFTLSMDDFVVTFFTAGPGQPTLPLQIYSMIRIAVTPEVNAISSILMML
TLAVSLTAYRVSRDVWRAQL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=248, Percent_Identity=41.5322580645161, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1787081, Length=275, Percent_Identity=39.6363636363636, Blast_Score=167, Evalue=5e-43,
Organism=Escherichia coli, GI1787714, Length=260, Percent_Identity=36.5384615384615, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786980, Length=118, Percent_Identity=38.135593220339, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28077; Mature: 28077

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPRWPVWVACLGCAVYAFLYLPLLIVVAYSFNDSRLNAEWDGFTLHWYDVLASDPDML
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHCCCHHH
GAAWNSLMIGAIAAGTSCVLGTLAAIALHRYRPPLLATLVLAPIAMPEVLMGVSLLLFFV
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVNIDLGLTAVALSHITFCVGFVAVVVRARLEGLDESLIDAARDLGASPLQAGWHVMLPL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHCCHHHHHHH
MLPGVLAGALLAFTLSMDDFVVTFFTAGPGQPTLPLQIYSMIRIAVTPEVNAISSILMML
HHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
TLAVSLTAYRVSRDVWRAQL
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRPRWPVWVACLGCAVYAFLYLPLLIVVAYSFNDSRLNAEWDGFTLHWYDVLASDPDML
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHCCCHHH
GAAWNSLMIGAIAAGTSCVLGTLAAIALHRYRPPLLATLVLAPIAMPEVLMGVSLLLFFV
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVNIDLGLTAVALSHITFCVGFVAVVVRARLEGLDESLIDAARDLGASPLQAGWHVMLPL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHCCHHHHHHH
MLPGVLAGALLAFTLSMDDFVVTFFTAGPGQPTLPLQIYSMIRIAVTPEVNAISSILMML
HHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
TLAVSLTAYRVSRDVWRAQL
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]