| Definition | Cupriavidus metallidurans CH34 megaplasmid, complete sequence. |
|---|---|
| Accession | NC_007974 |
| Length | 2,580,084 |
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The map label for this gene is tcuA [H]
Identifier: 94313137
GI number: 94313137
Start: 793633
End: 795066
Strand: Direct
Name: tcuA [H]
Synonym: Rmet_4210
Alternate gene names: 94313137
Gene position: 793633-795066 (Clockwise)
Preceding gene: 94313134
Following gene: 94313138
Centisome position: 30.76
GC content: 65.62
Gene sequence:
>1434_bases ATGATCGACGTCCTGGTGATCGGCGGCGGCAATGCCGCCCTTTGCGCCGCGCTGATGGCCCGTGAAGCGGGCGCCAGCGT GATGCTGCTGGAAGCCGCGCCGCGCGAATGGCGCGGCGGTAACTCCTCCCACACACGCAACCTGCGCTGCATGCATGACG CTCCGCAGGACGTGCTGGTCGACGCGTACCCCGAAGAAGAGTACTGGCAGGATCTGCTGAAAGTGACCGGCGGCCTGACC GACGAGAAGCTGGCGCGGCTTGCAATCCGCGCGTCGTCGTCGTGCCGGGACTGGATGCGCAGCCATGGCGTGCATTTCCA GCCACCGTTGTCTGGCGCGCTCCACGTGGCTCGCACCAATGCGTTCTTCATGGGCGGCGGCAAGGCGCTCGTCAATGCCT ACTACCGCAGTGCCGAGGCGCTTGGCGTGCAGATTCGCTACAACGCCCCAGTCGACGCCATCGAGCTCGATGGCAGCCGT TTCGTGGCGGCGCGTATCGGCGCAGAGCGGATTGAAGCACGCGCCTGCGTATTGGCGGCCGGCGGATTCGAATCAAACCG CGAGTGGCTGCGGGAGGCTTGGGGCCAGAACGAGCGGGGCGAATGGCCCGCCGACAACTTCCTGATCCGCGGCACCCGCT TCAACATGGGCGTGCTGCTCAAGCACATGATTGCCGCGGGTGCCGACGCAATCGGTGACCCGTCACAGTCGCACTGCGTG GCGATCGACGCGCGCGCGCCGCTCTACGACGGTGGCATCTGCACGCGAATCGATTGCGTCTCGCTCGGGGTCGTGCTCAA CGCCAATGCCGAGCGGTTCTACGACGAAGGCGAGGACTTCTGGCCCAAGCGCTATGCGATCTGGGGCCGTCTGGTCGCGC AGCAGCCACAGCAGATCGGTTACTCGATCATCGACGCAAAGGCGATCGGACGCTTCATGCCGCCGGTGTTCCCGGGCGTG AAGGCCGATACGCTGCCGGAACTCGCGCGCAAGCTCGGGCTCGACGAAGCCACGTTCATGCGGACGATCAACGCCTACAA CGCCGCGTGCCGGGTCGGCAAGTTCGACCACACGGTGCTGGACGATTGCCACACCGAGGGCGTGACGCCGTCCAAGACTC ACTGGGCGCGGCCGCTCGACACCGCGCCGTTCTTCGGCTACGCGCTTCGCCCAGGCATCACGTTCACGTACCTGGGACTG AAGGTCAACGAAGAGTCGGCCGTGCATTTCGATGGCAGGCCGAGTGACAACCTGTTCGTGGCCGGCGAGATGATGGCTGG CAACGTGCTCGGCAAGGGCTATACCGCTGGCGTCGGCATGGCGATCGGCACCGCGTTCGGCCGGATTGCCGGCACGCAGG CCGCCAAGGCGGCAAAGGGCATGGCCAGGCGTGCCTCACAGGCATCAACGGAAAAGGAATATGCAACATCTTGA
Upstream 100 bases:
>100_bases GGAGCAGGCGGATACAGTCCCGGACAGACTTCAGGAAGGCTCAGAAAGGCGTGTCAATTTCCACCCCCACCCCCAATCCC CGCACTCAGGACGCCCGGTC
Downstream 100 bases:
>100_bases AGCGCTGACGCGCGAGGCCGCGGCATTGGCGCGTGGCGCGCTGCCGCTGACTGCGGACGAGGGCGAAGTGGCCCGCACCC TGCAGATCTGCAACGCCTGC
Product: tricarballylate dehydrogenase
Products: NA
Alternate protein names: Fe(3+)-induced flavocytochrome C3; Ifc3; Iron(III)-induced flavocytochrome C3 [H]
Number of amino acids: Translated: 477; Mature: 477
Protein sequence:
>477_residues MIDVLVIGGGNAALCAALMAREAGASVMLLEAAPREWRGGNSSHTRNLRCMHDAPQDVLVDAYPEEEYWQDLLKVTGGLT DEKLARLAIRASSSCRDWMRSHGVHFQPPLSGALHVARTNAFFMGGGKALVNAYYRSAEALGVQIRYNAPVDAIELDGSR FVAARIGAERIEARACVLAAGGFESNREWLREAWGQNERGEWPADNFLIRGTRFNMGVLLKHMIAAGADAIGDPSQSHCV AIDARAPLYDGGICTRIDCVSLGVVLNANAERFYDEGEDFWPKRYAIWGRLVAQQPQQIGYSIIDAKAIGRFMPPVFPGV KADTLPELARKLGLDEATFMRTINAYNAACRVGKFDHTVLDDCHTEGVTPSKTHWARPLDTAPFFGYALRPGITFTYLGL KVNEESAVHFDGRPSDNLFVAGEMMAGNVLGKGYTAGVGMAIGTAFGRIAGTQAAKAAKGMARRASQASTEKEYATS
Sequences:
>Translated_477_residues MIDVLVIGGGNAALCAALMAREAGASVMLLEAAPREWRGGNSSHTRNLRCMHDAPQDVLVDAYPEEEYWQDLLKVTGGLT DEKLARLAIRASSSCRDWMRSHGVHFQPPLSGALHVARTNAFFMGGGKALVNAYYRSAEALGVQIRYNAPVDAIELDGSR FVAARIGAERIEARACVLAAGGFESNREWLREAWGQNERGEWPADNFLIRGTRFNMGVLLKHMIAAGADAIGDPSQSHCV AIDARAPLYDGGICTRIDCVSLGVVLNANAERFYDEGEDFWPKRYAIWGRLVAQQPQQIGYSIIDAKAIGRFMPPVFPGV KADTLPELARKLGLDEATFMRTINAYNAACRVGKFDHTVLDDCHTEGVTPSKTHWARPLDTAPFFGYALRPGITFTYLGL KVNEESAVHFDGRPSDNLFVAGEMMAGNVLGKGYTAGVGMAIGTAFGRIAGTQAAKAAKGMARRASQASTEKEYATS >Mature_477_residues MIDVLVIGGGNAALCAALMAREAGASVMLLEAAPREWRGGNSSHTRNLRCMHDAPQDVLVDAYPEEEYWQDLLKVTGGLT DEKLARLAIRASSSCRDWMRSHGVHFQPPLSGALHVARTNAFFMGGGKALVNAYYRSAEALGVQIRYNAPVDAIELDGSR FVAARIGAERIEARACVLAAGGFESNREWLREAWGQNERGEWPADNFLIRGTRFNMGVLLKHMIAAGADAIGDPSQSHCV AIDARAPLYDGGICTRIDCVSLGVVLNANAERFYDEGEDFWPKRYAIWGRLVAQQPQQIGYSIIDAKAIGRFMPPVFPGV KADTLPELARKLGLDEATFMRTINAYNAACRVGKFDHTVLDDCHTEGVTPSKTHWARPLDTAPFFGYALRPGITFTYLGL KVNEESAVHFDGRPSDNLFVAGEMMAGNVLGKGYTAGVGMAIGTAFGRIAGTQAAKAAKGMARRASQASTEKEYATS
Specific function: Catalyzes unidirectional fumarate reduction using artificial electron donors such as methyl viologen. The physiological reductant is unknown [H]
COG id: COG1053
COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 cytochrome c domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003953 - InterPro: IPR013027 - InterPro: IPR010960 - InterPro: IPR011031 [H]
Pfam domain/function: PF00890 FAD_binding_2 [H]
EC number: =1.3.99.1 [H]
Molecular weight: Translated: 51695; Mature: 51695
Theoretical pI: Translated: 7.07; Mature: 7.07
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.9 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 4.8 %Cys+Met (Translated Protein) 1.9 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 4.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIDVLVIGGGNAALCAALMAREAGASVMLLEAAPREWRGGNSSHTRNLRCMHDAPQDVLV CEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCEEEECCCCCCCEE DAYPEEEYWQDLLKVTGGLTDEKLARLAIRASSSCRDWMRSHGVHFQPPLSGALHVARTN ECCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCCEEEEECC AFFMGGGKALVNAYYRSAEALGVQIRYNAPVDAIELDGSRFVAARIGAERIEARACVLAA EEEECCCHHHHHHHHHCCHHEEEEEEECCCCCEEEECCCEEEEEHHCHHHHHHEEEEEEE GGFESNREWLREAWGQNERGEWPADNFLIRGTRFNMGVLLKHMIAAGADAIGDPSQSHCV CCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCEE AIDARAPLYDGGICTRIDCVSLGVVLNANAERFYDEGEDFWPKRYAIWGRLVAQQPQQIG EEECCCCEECCCCEEEEEEEEEEEEEECCHHHHHCCCCCCCCHHHHHHHHHHHCCHHHCC YSIIDAKAIGRFMPPVFPGVKADTLPELARKLGLDEATFMRTINAYNAACRVGKFDHTVL CCCEEHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHH DDCHTEGVTPSKTHWARPLDTAPFFGYALRPGITFTYLGLKVNEESAVHFDGRPSDNLFV HHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEEEECCCCEEEECCCCCCCEEE AGEMMAGNVLGKGYTAGVGMAIGTAFGRIAGTQAAKAAKGMARRASQASTEKEYATS EEEEEECCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHCCC >Mature Secondary Structure MIDVLVIGGGNAALCAALMAREAGASVMLLEAAPREWRGGNSSHTRNLRCMHDAPQDVLV CEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCEEEECCCCCCCEE DAYPEEEYWQDLLKVTGGLTDEKLARLAIRASSSCRDWMRSHGVHFQPPLSGALHVARTN ECCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCCEEEEECC AFFMGGGKALVNAYYRSAEALGVQIRYNAPVDAIELDGSRFVAARIGAERIEARACVLAA EEEECCCHHHHHHHHHCCHHEEEEEEECCCCCEEEECCCEEEEEHHCHHHHHHEEEEEEE GGFESNREWLREAWGQNERGEWPADNFLIRGTRFNMGVLLKHMIAAGADAIGDPSQSHCV CCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCEE AIDARAPLYDGGICTRIDCVSLGVVLNANAERFYDEGEDFWPKRYAIWGRLVAQQPQQIG EEECCCCEECCCCEEEEEEEEEEEEEECCHHHHHCCCCCCCCHHHHHHHHHHHCCHHHCC YSIIDAKAIGRFMPPVFPGVKADTLPELARKLGLDEATFMRTINAYNAACRVGKFDHTVL CCCEEHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHH DDCHTEGVTPSKTHWARPLDTAPFFGYALRPGITFTYLGLKVNEESAVHFDGRPSDNLFV HHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEEEECCCCEEEECCCCCCCEEE AGEMMAGNVLGKGYTAGVGMAIGTAFGRIAGTQAAKAAKGMARRASQASTEKEYATS EEEEEECCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10455032; 10581549 [H]