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The map label for this gene is dfrA [H]

Identifier: 94313097

GI number: 94313097

Start: 756509

End: 757585

Strand: Direct

Name: dfrA [H]

Synonym: Rmet_4170

Alternate gene names: 94313097

Gene position: 756509-757585 (Clockwise)

Preceding gene: 94313094

Following gene: 94313102

Centisome position: 29.32

GC content: 65.83

Gene sequence:

>1077_bases
ATGCTGCACCGCAATCCGGCTGTGGGTGGGCTTCTGCGGGGCAGAAGAGCCTGCCGCGCCGGCAGCATTGTCAGGGGATT
TGAAATTAACAATTGCATTCTTGTTACAGGTGCGTCGGGATTCCTGGGCTCCTCTGTGATGCGACAGGCACTCGACCGTG
GCTTCCAGGTGCGCGTGCTTGTGCGCTCGACCAGCCCGCGCGCCAATCTGGCCGGCTTGCCCGTGGAGATTGTCGAAGGT
GACATGCGCGATGCCGCGTCGATGACCCGCGCCATGGCCGGCGTGCGCTACCTGTTCCATGTGGCAGCCGATTACCGGCT
CTGGGCGCCTGATCCGGAAGAAATCGTGCGTTCGAACGTCGAGGGCACGGTCACGGTCATGAACGCCGCGCGCGCAGCTG
GCGTGGAGCGTGTCATCTATACGAGCAGCGTGGCCACCCTGCGTGTGGCGGGCGCCACGGCGCCGGTCGACGAGACGGCG
GCAATGGCCGGCCATGAGGCGATTGGCGCCTACAAGCGCAGCAAGGTGCTGGCCGAGCGCGAGGTGGAGCGTCTGGTGGC
GGAAGGCCTGCCGGCGGTCATCGTGAATCCCTCCACGCCGATTGGCCCGCGCGACGTGCGTCCGACTCCGACCGGCCGGA
TCATTGTCGAGGCGGCCACCGGCAAGATCCCGGCCTTCGTCGATACCGGCCTGAATCTGGTCCACGTGGACGACGTGGCG
AACGGTCATTTCCTGGCACTGGACAAAGGGCGCATCGGTGAGCGCTATATCCTGGGCGGCCAGGATGTGCTGCTGCGGCA
GATGCTGGCTGACATCGCGGACATGGCCGGCCGCAAGGCGCCAACCATCGAGTTGCCGCGCTGGCCGCTCTATCCGGTCG
CCCGCGTGGCCGAGACCATCGCGCGCTTCACCGGCAAGGAGCCGTTCGTGACCGTGGACGGGCTGAAGATGTCGCAATAT
CGGATGTTCTTCACTTCCGCCAAGGCCCAGCGTGAGCTTGGATACGCGCCGCGCCCCTATCAGGAAGGGCTGCGCGACGC
GCTCACGTGGTTCCGGGAAGCCGGCTATCTGAAGTAG

Upstream 100 bases:

>100_bases
TTCCCGGGATCACATTTCCGGACTACGGTGGTTACTATATACCGGTGTCAGTTTTCGTGTCGCCGCGTGGGAATTTGCCA
ACAGGGCGTTTCCGGCGAGA

Downstream 100 bases:

>100_bases
CCGATCGGCACGGCGGGGTGGCCCCGCCATCCGGACGTTACGACCGTACGGGCGCCTGCGAGCGCCCTTTCCATTGGCCG
CCACGACGCAGGTAATAGCG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MLHRNPAVGGLLRGRRACRAGSIVRGFEINNCILVTGASGFLGSSVMRQALDRGFQVRVLVRSTSPRANLAGLPVEIVEG
DMRDAASMTRAMAGVRYLFHVAADYRLWAPDPEEIVRSNVEGTVTVMNAARAAGVERVIYTSSVATLRVAGATAPVDETA
AMAGHEAIGAYKRSKVLAEREVERLVAEGLPAVIVNPSTPIGPRDVRPTPTGRIIVEAATGKIPAFVDTGLNLVHVDDVA
NGHFLALDKGRIGERYILGGQDVLLRQMLADIADMAGRKAPTIELPRWPLYPVARVAETIARFTGKEPFVTVDGLKMSQY
RMFFTSAKAQRELGYAPRPYQEGLRDALTWFREAGYLK

Sequences:

>Translated_358_residues
MLHRNPAVGGLLRGRRACRAGSIVRGFEINNCILVTGASGFLGSSVMRQALDRGFQVRVLVRSTSPRANLAGLPVEIVEG
DMRDAASMTRAMAGVRYLFHVAADYRLWAPDPEEIVRSNVEGTVTVMNAARAAGVERVIYTSSVATLRVAGATAPVDETA
AMAGHEAIGAYKRSKVLAEREVERLVAEGLPAVIVNPSTPIGPRDVRPTPTGRIIVEAATGKIPAFVDTGLNLVHVDDVA
NGHFLALDKGRIGERYILGGQDVLLRQMLADIADMAGRKAPTIELPRWPLYPVARVAETIARFTGKEPFVTVDGLKMSQY
RMFFTSAKAQRELGYAPRPYQEGLRDALTWFREAGYLK
>Mature_358_residues
MLHRNPAVGGLLRGRRACRAGSIVRGFEINNCILVTGASGFLGSSVMRQALDRGFQVRVLVRSTSPRANLAGLPVEIVEG
DMRDAASMTRAMAGVRYLFHVAADYRLWAPDPEEIVRSNVEGTVTVMNAARAAGVERVIYTSSVATLRVAGATAPVDETA
AMAGHEAIGAYKRSKVLAEREVERLVAEGLPAVIVNPSTPIGPRDVRPTPTGRIIVEAATGKIPAFVDTGLNLVHVDDVA
NGHFLALDKGRIGERYILGGQDVLLRQMLADIADMAGRKAPTIELPRWPLYPVARVAETIARFTGKEPFVTVDGLKMSQY
RMFFTSAKAQRELGYAPRPYQEGLRDALTWFREAGYLK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI116268111, Length=355, Percent_Identity=25.6338028169014, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI239745448, Length=341, Percent_Identity=27.8592375366569, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI310132178, Length=341, Percent_Identity=27.5659824046921, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI310113012, Length=341, Percent_Identity=27.5659824046921, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI193211614, Length=326, Percent_Identity=26.0736196319018, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI8393516, Length=326, Percent_Identity=26.0736196319018, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71987463, Length=349, Percent_Identity=27.7936962750716, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 38866; Mature: 38866

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHRNPAVGGLLRGRRACRAGSIVRGFEINNCILVTGASGFLGSSVMRQALDRGFQVRVL
CCCCCCCHHHHHHHHHHHHCCCEEECEEECCEEEEECCCCHHHHHHHHHHHCCCCEEEEE
VRSTSPRANLAGLPVEIVEGDMRDAASMTRAMAGVRYLFHVAADYRLWAPDPEEIVRSNV
EECCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHCCC
EGTVTVMNAARAAGVERVIYTSSVATLRVAGATAPVDETAAMAGHEAIGAYKRSKVLAER
CCEEEEEHHHHHHCCHHEEEECCEEEEEEECCCCCCHHHHHHHCHHHHHHHHHHHHHHHH
EVERLVAEGLPAVIVNPSTPIGPRDVRPTPTGRIIVEAATGKIPAFVDTGLNLVHVDDVA
HHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHCCCEEEEEECCC
NGHFLALDKGRIGERYILGGQDVLLRQMLADIADMAGRKAPTIELPRWPLYPVARVAETI
CCEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHH
ARFTGKEPFVTVDGLKMSQYRMFFTSAKAQRELGYAPRPYQEGLRDALTWFREAGYLK
HHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLHRNPAVGGLLRGRRACRAGSIVRGFEINNCILVTGASGFLGSSVMRQALDRGFQVRVL
CCCCCCCHHHHHHHHHHHHCCCEEECEEECCEEEEECCCCHHHHHHHHHHHCCCCEEEEE
VRSTSPRANLAGLPVEIVEGDMRDAASMTRAMAGVRYLFHVAADYRLWAPDPEEIVRSNV
EECCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHCCC
EGTVTVMNAARAAGVERVIYTSSVATLRVAGATAPVDETAAMAGHEAIGAYKRSKVLAER
CCEEEEEHHHHHHCCHHEEEECCEEEEEEECCCCCCHHHHHHHCHHHHHHHHHHHHHHHH
EVERLVAEGLPAVIVNPSTPIGPRDVRPTPTGRIIVEAATGKIPAFVDTGLNLVHVDDVA
HHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHCCCEEEEEECCC
NGHFLALDKGRIGERYILGGQDVLLRQMLADIADMAGRKAPTIELPRWPLYPVARVAETI
CCEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHH
ARFTGKEPFVTVDGLKMSQYRMFFTSAKAQRELGYAPRPYQEGLRDALTWFREAGYLK
HHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]