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The map label for this gene is mlaA [H]

Identifier: 94313092

GI number: 94313092

Start: 749600

End: 750475

Strand: Reverse

Name: mlaA [H]

Synonym: Rmet_4165

Alternate gene names: 94313092

Gene position: 750475-749600 (Counterclockwise)

Preceding gene: 94313093

Following gene: 94313080

Centisome position: 29.09

GC content: 65.87

Gene sequence:

>876_bases
ATGCTATCCCGCTTTTCGCCTTCGGGCGCCGTAGCACTGCGCCAGCCCCAGCTTGCCCGCACGCTGGCCGCCGTCTCGCT
CTCTGTCCTCGCCACCGGCTGCGCCACGGGTCCTCAAGCCAATCCGCAGGATCCGCTGGAGCCCATGAACCGGGTGATCT
TCAAGGTCAACGACACGATCGACACCTATGGCGCGCAACCGCTGGCCAAGGGCTACAAGGCGGTGACGCCAGAGCCGGCC
CGTCTGGCCGTGACCAATTTCTTCTCGAACCTGTCTGACGTGGGCAACTTCGTCAACAACGTGCTGCAGGGCAAGGGCGT
CGATGCCACCGAAACGATGATGCGCATCGCCATCAATTCGGTGCTCGGCATTGGCGGCCTGATCGACGTGGCGACGGCCG
CTGGCCTGAACCGCCATTCGCAGGATTTCGGGCTGACCCTCGGTACCTGGGGCGTGCCCGCTGGCCCATACCTGGTGCTG
CCGATCTACGGACCGAGTTCGTTCCGCGACAGCATGGGCGTAGCCGCCAGCATCTACCTGGACCCGGCGCACTATCTGGA
TCCGGCCTGGCGCAACTCGCTGTTCGGCGTCAACGTGATCAACACGCGCTCGAACCTGCTGGGCGCCACGGACCTGCTCT
CGCAGGCCGCGCTCGACAAGTACGCGTTCGTGCGCGATGCCTATTCGCAGCGGCGCCAGTACCTGCTGAACCAGGGTGAA
AACGCGCTGCCGTCGTACGACGAGGTGGACGAGTCGGACAAGGCCTCGGAGGCCAAGCCCGCTGCTGGGGAGGCCGCCCC
GGCAACTCCGGCAACTCCGGCAACTCCGGCAACTCCGGCAACCCCGGCTGCCCCGCCATCCGACTCAGCAAAGTAG

Upstream 100 bases:

>100_bases
TGTCACAATCTTCCGATAACACAGCGGGGCCGGATCAACGCCACCCAGGAAGGTAGGCGGCCGCACCCTTACAAGAAACC
AAAGCCAAGACCGTTTCCCG

Downstream 100 bases:

>100_bases
CCAGAATAGCGTCTCGGGGCAATCGAGACGCTTGCCCTCTCCACCACCCCATAGAGGGCATATCAATGGCTGCTGGTTGG
CTCTCCGGCTGAGAGCGTCA

Product: lipoprotein vacJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLSRFSPSGAVALRQPQLARTLAAVSLSVLATGCATGPQANPQDPLEPMNRVIFKVNDTIDTYGAQPLAKGYKAVTPEPA
RLAVTNFFSNLSDVGNFVNNVLQGKGVDATETMMRIAINSVLGIGGLIDVATAAGLNRHSQDFGLTLGTWGVPAGPYLVL
PIYGPSSFRDSMGVAASIYLDPAHYLDPAWRNSLFGVNVINTRSNLLGATDLLSQAALDKYAFVRDAYSQRRQYLLNQGE
NALPSYDEVDESDKASEAKPAAGEAAPATPATPATPATPATPAAPPSDSAK

Sequences:

>Translated_291_residues
MLSRFSPSGAVALRQPQLARTLAAVSLSVLATGCATGPQANPQDPLEPMNRVIFKVNDTIDTYGAQPLAKGYKAVTPEPA
RLAVTNFFSNLSDVGNFVNNVLQGKGVDATETMMRIAINSVLGIGGLIDVATAAGLNRHSQDFGLTLGTWGVPAGPYLVL
PIYGPSSFRDSMGVAASIYLDPAHYLDPAWRNSLFGVNVINTRSNLLGATDLLSQAALDKYAFVRDAYSQRRQYLLNQGE
NALPSYDEVDESDKASEAKPAAGEAAPATPATPATPATPATPAAPPSDSAK
>Mature_291_residues
MLSRFSPSGAVALRQPQLARTLAAVSLSVLATGCATGPQANPQDPLEPMNRVIFKVNDTIDTYGAQPLAKGYKAVTPEPA
RLAVTNFFSNLSDVGNFVNNVLQGKGVDATETMMRIAINSVLGIGGLIDVATAAGLNRHSQDFGLTLGTWGVPAGPYLVL
PIYGPSSFRDSMGVAASIYLDPAHYLDPAWRNSLFGVNVINTRSNLLGATDLLSQAALDKYAFVRDAYSQRRQYLLNQGE
NALPSYDEVDESDKASEAKPAAGEAAPATPATPATPATPATPAAPPSDSAK

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=226, Percent_Identity=36.7256637168142, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 30520; Mature: 30520

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRFSPSGAVALRQPQLARTLAAVSLSVLATGCATGPQANPQDPLEPMNRVIFKVNDTI
CCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEECCCH
DTYGAQPLAKGYKAVTPEPARLAVTNFFSNLSDVGNFVNNVLQGKGVDATETMMRIAINS
HHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VLGIGGLIDVATAAGLNRHSQDFGLTLGTWGVPAGPYLVLPIYGPSSFRDSMGVAASIYL
HHHHHHHHHHHHHHCCCCCCHHHCEEEECCCCCCCCEEEEEECCCCHHHHHCCCEEEEEE
DPAHYLDPAWRNSLFGVNVINTRSNLLGATDLLSQAALDKYAFVRDAYSQRRQYLLNQGE
CCHHHCCHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NALPSYDEVDESDKASEAKPAAGEAAPATPATPATPATPATPAAPPSDSAK
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLSRFSPSGAVALRQPQLARTLAAVSLSVLATGCATGPQANPQDPLEPMNRVIFKVNDTI
CCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEECCCH
DTYGAQPLAKGYKAVTPEPARLAVTNFFSNLSDVGNFVNNVLQGKGVDATETMMRIAINS
HHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VLGIGGLIDVATAAGLNRHSQDFGLTLGTWGVPAGPYLVLPIYGPSSFRDSMGVAASIYL
HHHHHHHHHHHHHHCCCCCCHHHCEEEECCCCCCCCEEEEEECCCCHHHHHCCCEEEEEE
DPAHYLDPAWRNSLFGVNVINTRSNLLGATDLLSQAALDKYAFVRDAYSQRRQYLLNQGE
CCHHHCCHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NALPSYDEVDESDKASEAKPAAGEAAPATPATPATPATPATPAAPPSDSAK
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]