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The map label for this gene is tnpA [H]

Identifier: 94313081

GI number: 94313081

Start: 729826

End: 731034

Strand: Direct

Name: tnpA [H]

Synonym: Rmet_4154

Alternate gene names: 94313081

Gene position: 729826-731034 (Clockwise)

Preceding gene: 94313070

Following gene: 94313082

Centisome position: 28.29

GC content: 69.07

Gene sequence:

>1209_bases
ATGAGGGTGATGGCCAGAACACGCAAGACGCTGCCGTCGAGAGTTGACGTTGCGCATTTGATCAGCGCCGGGGTGCTGGC
CAGCGTGTGTCCACGGACGTTGATCGAAGAAGTGTTGGCCGAGACCGGCAAGGCCAGCCAGCGGGAGCGGCTGCTGCCCG
CGCCGGCAGTGGTGTATTACGTGATGGCGCTGGCGCTGTGGCGCGAAGCGCCGCTGGAGGAAGTGCTGCGCGTGGTGTGC
GAAGGCCTGCAATGGCTCGGCGGCGGCCACACTGAGGCGGTGCAGGCCAGCAAGTCCGCGATCTCGCAGGCGCGCAGCCG
GCTGGGCCCCGAGGTCATGCGCCAATTGGCCGATCGGGTGCTGCGCCCGCTGGCCGCGCCGGGGGCGCCAGGGGCGTGGT
ACCGGGGATTGCGCGTGATGGCCCTGGACGGCAGCTGCATGGACGTGGCCGACGAGGCGGCCAACGCCAAGTTCTTCGGC
TACCCGGGCGCCTCGCGCGGCCAAAGCGCCTTCCCGCAGGCGCGCGTTCTGGGCCTGGTGGAATGCGGCACGCACGCGGT
GGTGGCAGCCGGCATCGCACCCTACGGCCACAGCGAGCAGGTCATGGCCGCCCAGTTGCTGCCCGCCAAGCTCACGCCCG
AGATGCTGGTGCTGGCCGACCGCAACTTCTACGGCTTCAAGCTCTGGCAGACCGCCTGCGCTACGGGCGCCAAGCTGGCC
TGGCGGGTCAAATCCAACCTCAAGCTGCCGGTAGAGCAGATGTTGCCCGATGGCTCGTACCTGAGCCGCGTGTTCGACAG
CGACGACCGCGCGCGGCGCGCAGGGCAAACGGTGCGTGTGATCGACTACGCGCTCGAGGGCTCGGCCACGCCGGCGCAGG
GCAGCTACCGGCTGCTGACCAACCTCCTGGACCCCGATGCGGCGCCGGCCCTGGAGCTGGCCGCGCTGTACCACGAGCGC
TGGGAGATCGAGGGGGTGTTCGATGAGTTCAAGACGCATTTGCGCGCCAACAGCACGGTGCTGCGCAGCAAGACGCCCGA
GCTGGTGCAGCAGGAGCTGTGGGGGCTGCTGCTGGCGCACTTTGCGATTCGCCAGTTGATGGCGCAGGCGGCCTGGGCGC
GCGAACTCGATCCGGATCGCCTGAGCTTCATGCACGCCGTGCGCGTGATCAAGCGCAAGATGCCGCAAGCTGCGGCCGTT
TCCCCCTGA

Upstream 100 bases:

>100_bases
CCGCCATCGGCAACCGTCATAACAGCAGCGGACTTTAAGCCCAATACTGTTCAGATAGTATTTTTAAAGCCATAATCCTC
ATCCATCGAAGCTTCAATGG

Downstream 100 bases:

>100_bases
GCGTTTGCCTGCCTGGCGCGAGGCCCTGCTGGCCGAGATCGCGCGCGGGCGCTGCGTGAGCAGTCGTGGGCGCCTGAACC
CGCGCGGCGTCAAGCGCAAG

Product: transposase ISRme8

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MRVMARTRKTLPSRVDVAHLISAGVLASVCPRTLIEEVLAETGKASQRERLLPAPAVVYYVMALALWREAPLEEVLRVVC
EGLQWLGGGHTEAVQASKSAISQARSRLGPEVMRQLADRVLRPLAAPGAPGAWYRGLRVMALDGSCMDVADEAANAKFFG
YPGASRGQSAFPQARVLGLVECGTHAVVAAGIAPYGHSEQVMAAQLLPAKLTPEMLVLADRNFYGFKLWQTACATGAKLA
WRVKSNLKLPVEQMLPDGSYLSRVFDSDDRARRAGQTVRVIDYALEGSATPAQGSYRLLTNLLDPDAAPALELAALYHER
WEIEGVFDEFKTHLRANSTVLRSKTPELVQQELWGLLLAHFAIRQLMAQAAWARELDPDRLSFMHAVRVIKRKMPQAAAV
SP

Sequences:

>Translated_402_residues
MRVMARTRKTLPSRVDVAHLISAGVLASVCPRTLIEEVLAETGKASQRERLLPAPAVVYYVMALALWREAPLEEVLRVVC
EGLQWLGGGHTEAVQASKSAISQARSRLGPEVMRQLADRVLRPLAAPGAPGAWYRGLRVMALDGSCMDVADEAANAKFFG
YPGASRGQSAFPQARVLGLVECGTHAVVAAGIAPYGHSEQVMAAQLLPAKLTPEMLVLADRNFYGFKLWQTACATGAKLA
WRVKSNLKLPVEQMLPDGSYLSRVFDSDDRARRAGQTVRVIDYALEGSATPAQGSYRLLTNLLDPDAAPALELAALYHER
WEIEGVFDEFKTHLRANSTVLRSKTPELVQQELWGLLLAHFAIRQLMAQAAWARELDPDRLSFMHAVRVIKRKMPQAAAV
SP
>Mature_402_residues
MRVMARTRKTLPSRVDVAHLISAGVLASVCPRTLIEEVLAETGKASQRERLLPAPAVVYYVMALALWREAPLEEVLRVVC
EGLQWLGGGHTEAVQASKSAISQARSRLGPEVMRQLADRVLRPLAAPGAPGAWYRGLRVMALDGSCMDVADEAANAKFFG
YPGASRGQSAFPQARVLGLVECGTHAVVAAGIAPYGHSEQVMAAQLLPAKLTPEMLVLADRNFYGFKLWQTACATGAKLA
WRVKSNLKLPVEQMLPDGSYLSRVFDSDDRARRAGQTVRVIDYALEGSATPAQGSYRLLTNLLDPDAAPALELAALYHER
WEIEGVFDEFKTHLRANSTVLRSKTPELVQQELWGLLLAHFAIRQLMAQAAWARELDPDRLSFMHAVRVIKRKMPQAAAV
SP

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=377, Percent_Identity=28.6472148541114, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 44011; Mature: 44011

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVMARTRKTLPSRVDVAHLISAGVLASVCPRTLIEEVLAETGKASQRERLLPAPAVVYY
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHH
VMALALWREAPLEEVLRVVCEGLQWLGGGHTEAVQASKSAISQARSRLGPEVMRQLADRV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LRPLAAPGAPGAWYRGLRVMALDGSCMDVADEAANAKFFGYPGASRGQSAFPQARVLGLV
HHHHCCCCCCCHHHHCEEEEEECCCHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHH
ECGTHAVVAAGIAPYGHSEQVMAAQLLPAKLTPEMLVLADRNFYGFKLWQTACATGAKLA
HCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEE
WRVKSNLKLPVEQMLPDGSYLSRVFDSDDRARRAGQTVRVIDYALEGSATPAQGSYRLLT
EEECCCCCCCHHHHCCCHHHHHHHHCCCHHHHHCCCEEEEHHHHHCCCCCCCCCHHHHHH
NLLDPDAAPALELAALYHERWEIEGVFDEFKTHLRANSTVLRSKTPELVQQELWGLLLAH
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHH
FAIRQLMAQAAWARELDPDRLSFMHAVRVIKRKMPQAAAVSP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRVMARTRKTLPSRVDVAHLISAGVLASVCPRTLIEEVLAETGKASQRERLLPAPAVVYY
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHH
VMALALWREAPLEEVLRVVCEGLQWLGGGHTEAVQASKSAISQARSRLGPEVMRQLADRV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LRPLAAPGAPGAWYRGLRVMALDGSCMDVADEAANAKFFGYPGASRGQSAFPQARVLGLV
HHHHCCCCCCCHHHHCEEEEEECCCHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHH
ECGTHAVVAAGIAPYGHSEQVMAAQLLPAKLTPEMLVLADRNFYGFKLWQTACATGAKLA
HCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEE
WRVKSNLKLPVEQMLPDGSYLSRVFDSDDRARRAGQTVRVIDYALEGSATPAQGSYRLLT
EEECCCCCCCHHHHCCCHHHHHHHHCCCHHHHHCCCEEEEHHHHHCCCCCCCCCHHHHHH
NLLDPDAAPALELAALYHERWEIEGVFDEFKTHLRANSTVLRSKTPELVQQELWGLLLAH
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHH
FAIRQLMAQAAWARELDPDRLSFMHAVRVIKRKMPQAAAVSP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]