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The map label for this gene is dosC [H]

Identifier: 94313070

GI number: 94313070

Start: 717351

End: 718580

Strand: Direct

Name: dosC [H]

Synonym: Rmet_4143

Alternate gene names: 94313070

Gene position: 717351-718580 (Clockwise)

Preceding gene: 94313069

Following gene: 94313081

Centisome position: 27.8

GC content: 67.15

Gene sequence:

>1230_bases
GTGCACCTGGCCCAGCAGACCATCCTGGTCGTGATGATGGTGGTTTTCTCCAGCACGCTCGTCATGGCGGCGGGGCTGGT
GCTCGCGCTGCGCGCCAATGAGGCTGGCCGCCTGTGGGCCGTCGGGCATGTGATCGCCTCGGGCGCGGGGCTGGTCATCG
CGGCCAGCGCAAGCGCCGGATCGGCGGAACTCGGCGCGCTCGGCGCCGGTGCCTATGTGTTTGGCCGCCTGACGATCTAC
CGGGGTGTGCGCGTCTACTACGGCATGTCCGAACACGCGGCGCTGCTGCACGGTGTCGGCTTCGTGACGCTGGTGCTGCT
GATGCTGCTGACCGGCCTGCCCGATGGCAGCAGGCTCGTGCATGGCCTCGCGTACGGCTTGCTGGCCCTGATCTCGTTTT
TCACTGTCATCACCATGCTGCGCAGCGCGCGCGGCCGCAGGAGCATCGGCAGCCCGCTGGTGCTGGCCTCCCACCTGGTG
CTGCTGATCTCGCAGGTGGCGGCGCTGAGCTACGGGGCGACATTCAAGGGTTTCGAGGTCACGCCTTTCTTCCTGCAGCC
CGAAGGCTCGGTCTGGCTGCTGGCGCCGCTGGTGGCCGTGTTGCTGGGCCTGTTCGGTTTCTGCCTGATGTCGATGGAGC
AGATCATCGCGCGCAACGAGAGCGGCGCGCGTACCGATGCGCTGACCGGGCTGCTCAATCGCGGCGCGCTCGACATGCTG
GCCATCAGCATGATCGCGCGCTGGCAGCGCAACGGCGAGGCGCTGTCGTGCCTCGTGATCGACGTCGACTATTTCAAGCT
GGTCAACGACACGCACGGCCACCATGCCGGTGACAACGTGCTGCGTGAAATCGCGACGGCGCTAGACAATTCGCGGCGCG
CGTCGGATATTGCCGCGCGCTACGGTGGCGAGGAGTTTTGCATCCTCTGTCCGCATACGGACGAGCACCAGGCCACTGCG
CTGGCCAATCGTATTCTGCGGAAGGTGAGGGCGATCGAACTGCCCGGCGCGGGCGGTCATGCCAGCGTCAGCATCGGCGT
GGCCGAGCTGCGCGGCGGGCCCTCGAGCCGTGAGGGCCTGTGGCGCAGCCTGTTCGCCACGGCGGACCGCGCGCTCTATC
AGGCCAAGCAGCGCGGCCGCGACCAGTTCATGCTGGCGTCCAGCATGATCGAAGAGACACCGGTCGGCGACGCGCTGCAA
TCGGTGACCGAGCCGCAGATGATTGTTTGA

Upstream 100 bases:

>100_bases
TGCGTGCTTTCGTGCCGACGATAGCAACAGGGGCTACCCGTCAAGGGTAAACCTCGACAAGCGGCAGCCGCCCCCGGTAG
CTTTAGAGGATTCGACTCCC

Downstream 100 bases:

>100_bases
CGGCGGATACCCAGGCGGGCCCGGTGGGTACCTGGGCCGTCCTTATACCGCCCCGCCATCCACCGGCACATCCCGTTGCG
GCTGCCCGTCGTACGTCCAG

Product: putative diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MHLAQQTILVVMMVVFSSTLVMAAGLVLALRANEAGRLWAVGHVIASGAGLVIAASASAGSAELGALGAGAYVFGRLTIY
RGVRVYYGMSEHAALLHGVGFVTLVLLMLLTGLPDGSRLVHGLAYGLLALISFFTVITMLRSARGRRSIGSPLVLASHLV
LLISQVAALSYGATFKGFEVTPFFLQPEGSVWLLAPLVAVLLGLFGFCLMSMEQIIARNESGARTDALTGLLNRGALDML
AISMIARWQRNGEALSCLVIDVDYFKLVNDTHGHHAGDNVLREIATALDNSRRASDIAARYGGEEFCILCPHTDEHQATA
LANRILRKVRAIELPGAGGHASVSIGVAELRGGPSSREGLWRSLFATADRALYQAKQRGRDQFMLASSMIEETPVGDALQ
SVTEPQMIV

Sequences:

>Translated_409_residues
MHLAQQTILVVMMVVFSSTLVMAAGLVLALRANEAGRLWAVGHVIASGAGLVIAASASAGSAELGALGAGAYVFGRLTIY
RGVRVYYGMSEHAALLHGVGFVTLVLLMLLTGLPDGSRLVHGLAYGLLALISFFTVITMLRSARGRRSIGSPLVLASHLV
LLISQVAALSYGATFKGFEVTPFFLQPEGSVWLLAPLVAVLLGLFGFCLMSMEQIIARNESGARTDALTGLLNRGALDML
AISMIARWQRNGEALSCLVIDVDYFKLVNDTHGHHAGDNVLREIATALDNSRRASDIAARYGGEEFCILCPHTDEHQATA
LANRILRKVRAIELPGAGGHASVSIGVAELRGGPSSREGLWRSLFATADRALYQAKQRGRDQFMLASSMIEETPVGDALQ
SVTEPQMIV
>Mature_409_residues
MHLAQQTILVVMMVVFSSTLVMAAGLVLALRANEAGRLWAVGHVIASGAGLVIAASASAGSAELGALGAGAYVFGRLTIY
RGVRVYYGMSEHAALLHGVGFVTLVLLMLLTGLPDGSRLVHGLAYGLLALISFFTVITMLRSARGRRSIGSPLVLASHLV
LLISQVAALSYGATFKGFEVTPFFLQPEGSVWLLAPLVAVLLGLFGFCLMSMEQIIARNESGARTDALTGLLNRGALDML
AISMIARWQRNGEALSCLVIDVDYFKLVNDTHGHHAGDNVLREIATALDNSRRASDIAARYGGEEFCILCPHTDEHQATA
LANRILRKVRAIELPGAGGHASVSIGVAELRGGPSSREGLWRSLFATADRALYQAKQRGRDQFMLASSMIEETPVGDALQ
SVTEPQMIV

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=207, Percent_Identity=37.6811594202899, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=38.6363636363636, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=39.375, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1786584, Length=244, Percent_Identity=26.6393442622951, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787816, Length=196, Percent_Identity=33.6734693877551, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787541, Length=167, Percent_Identity=35.3293413173653, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87081974, Length=186, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787802, Length=135, Percent_Identity=28.8888888888889, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788381, Length=109, Percent_Identity=37.6146788990826, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=164, Percent_Identity=28.0487804878049, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 43552; Mature: 43552

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLAQQTILVVMMVVFSSTLVMAAGLVLALRANEAGRLWAVGHVIASGAGLVIAASASAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHCCCCEEEEECCCCC
SAELGALGAGAYVFGRLTIYRGVRVYYGMSEHAALLHGVGFVTLVLLMLLTGLPDGSRLV
CCHHHHCCCCHHHHHHHHHHHCHHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
HGLAYGLLALISFFTVITMLRSARGRRSIGSPLVLASHLVLLISQVAALSYGATFKGFEV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
TPFFLQPEGSVWLLAPLVAVLLGLFGFCLMSMEQIIARNESGARTDALTGLLNRGALDML
EEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHH
AISMIARWQRNGEALSCLVIDVDYFKLVNDTHGHHAGDNVLREIATALDNSRRASDIAAR
HHHHHHHHHCCCCEEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
YGGEEFCILCPHTDEHQATALANRILRKVRAIELPGAGGHASVSIGVAELRGGPSSREGL
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEHHHHCCCCCCHHHH
WRSLFATADRALYQAKQRGRDQFMLASSMIEETPVGDALQSVTEPQMIV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MHLAQQTILVVMMVVFSSTLVMAAGLVLALRANEAGRLWAVGHVIASGAGLVIAASASAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHCCCCEEEEECCCCC
SAELGALGAGAYVFGRLTIYRGVRVYYGMSEHAALLHGVGFVTLVLLMLLTGLPDGSRLV
CCHHHHCCCCHHHHHHHHHHHCHHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
HGLAYGLLALISFFTVITMLRSARGRRSIGSPLVLASHLVLLISQVAALSYGATFKGFEV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
TPFFLQPEGSVWLLAPLVAVLLGLFGFCLMSMEQIIARNESGARTDALTGLLNRGALDML
EEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHH
AISMIARWQRNGEALSCLVIDVDYFKLVNDTHGHHAGDNVLREIATALDNSRRASDIAAR
HHHHHHHHHCCCCEEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
YGGEEFCILCPHTDEHQATALANRILRKVRAIELPGAGGHASVSIGVAELRGGPSSREGL
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEHHHHCCCCCCHHHH
WRSLFATADRALYQAKQRGRDQFMLASSMIEETPVGDALQSVTEPQMIV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]