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The map label for this gene is fucA [C]

Identifier: 94313035

GI number: 94313035

Start: 680121

End: 680897

Strand: Reverse

Name: fucA [C]

Synonym: Rmet_4108

Alternate gene names: 94313035

Gene position: 680897-680121 (Counterclockwise)

Preceding gene: 94313036

Following gene: 94313031

Centisome position: 26.39

GC content: 62.68

Gene sequence:

>777_bases
ATGGCCAATACCATGCAGTTGAGCAAGAGCGAAATCGTCGCGTCTTCGCTCGAAAAAATGCAGACCGAACTCCGCGCGGA
CAGGATGCCGCTGCGCGAAGCTGTCGCCGAGACCTGCCGGATCCTGTTCACGTTCGGGCACGATTCGGTGCTCTCCGGCC
AGATCACGGCGCGGGCGGAACAACCGGGCACGTACTACACGCAACGCCTGGGTCTTGGCTTCGACGAAATCACGGCGGAC
AACCTCCTGCTCGTCAACGAAGATCTTGAGGTGATTTCCGGAAACGGGATGCCAAACCCCGCCAATCGCTTCCATTCATG
GCTCTATCGCGCGCGTCCCGATTTGAATTGCATCGTTCACTCTCACGCGCCGCACGCTTCCGCGCTTGCCATGCTGGAAG
TGCCGCTGACGATCTCGCACATGGACACGTGCGTGCTGTACGACCAGACCGCGTTCCTGGAAAAATGGCCGGGCATTCCA
GTCGGCAATGAAGAGGGCGAACTCATCGCGGGCGCCCTGGGCGACAAGAAGGCGATTCTGCTGTCGCACCACGGCCTGCT
GGTAGCTGGCGCGTCGGTGGAAGAAGCCTGTGTGCTGGGCGTGATGTTCGAGCGCGCCGCTCGCCTGCAACTGCTGGCGA
TGGCCGCCGGGGAGATCCAGCCGATCCCGCCCGAACTCGGTCGCGAGGCGCAACGCTGGGTCAGCACGCAGAAGCGATTC
GAAGCGACTTTCGCCTACTATTCGCGCCGCGCGCGCCGCCAGCTCGACGCGCAGTAA

Upstream 100 bases:

>100_bases
GATCAGCGACGAGAATCAGCGATGGTCTGCCCTCCTTGGCGCAGGCAACAAGTAAAGCCACCTCCTACCCCGACTCCAGC
ACATCCCTGAAGGACACAAC

Downstream 100 bases:

>100_bases
GGTTGGCGTAGTCCCCTCGACTACCGTGACGCCGCTGCCCGACCAGGCGGCTAGGCGGCAGCGTCGTGGACGATGCTGCC
GGTCGCCGGCGTATCTCTGC

Product: class II aldolase/adducin domain-containing protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MANTMQLSKSEIVASSLEKMQTELRADRMPLREAVAETCRILFTFGHDSVLSGQITARAEQPGTYYTQRLGLGFDEITAD
NLLLVNEDLEVISGNGMPNPANRFHSWLYRARPDLNCIVHSHAPHASALAMLEVPLTISHMDTCVLYDQTAFLEKWPGIP
VGNEEGELIAGALGDKKAILLSHHGLLVAGASVEEACVLGVMFERAARLQLLAMAAGEIQPIPPELGREAQRWVSTQKRF
EATFAYYSRRARRQLDAQ

Sequences:

>Translated_258_residues
MANTMQLSKSEIVASSLEKMQTELRADRMPLREAVAETCRILFTFGHDSVLSGQITARAEQPGTYYTQRLGLGFDEITAD
NLLLVNEDLEVISGNGMPNPANRFHSWLYRARPDLNCIVHSHAPHASALAMLEVPLTISHMDTCVLYDQTAFLEKWPGIP
VGNEEGELIAGALGDKKAILLSHHGLLVAGASVEEACVLGVMFERAARLQLLAMAAGEIQPIPPELGREAQRWVSTQKRF
EATFAYYSRRARRQLDAQ
>Mature_257_residues
ANTMQLSKSEIVASSLEKMQTELRADRMPLREAVAETCRILFTFGHDSVLSGQITARAEQPGTYYTQRLGLGFDEITADN
LLLVNEDLEVISGNGMPNPANRFHSWLYRARPDLNCIVHSHAPHASALAMLEVPLTISHMDTCVLYDQTAFLEKWPGIPV
GNEEGELIAGALGDKKAILLSHHGLLVAGASVEEACVLGVMFERAARLQLLAMAAGEIQPIPPELGREAQRWVSTQKRFE
ATFAYYSRRARRQLDAQ

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI29826323, Length=172, Percent_Identity=32.5581395348837, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI29826325, Length=192, Percent_Identity=30.7291666666667, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI29826321, Length=172, Percent_Identity=32.5581395348837, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI29826319, Length=172, Percent_Identity=32.5581395348837, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789164, Length=204, Percent_Identity=28.4313725490196, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI115533216, Length=191, Percent_Identity=25.6544502617801, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115533218, Length=196, Percent_Identity=25, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281363755, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281363751, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24655690, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24655685, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281363763, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281363757, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI17136738, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281363761, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI281363759, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI281363753, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 28530; Mature: 28399

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTMQLSKSEIVASSLEKMQTELRADRMPLREAVAETCRILFTFGHDSVLSGQITARAE
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCEECCEEEEECC
QPGTYYTQRLGLGFDEITADNLLLVNEDLEVISGNGMPNPANRFHSWLYRARPDLNCIVH
CCCHHHHHHHCCCHHHHCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEE
SHAPHASALAMLEVPLTISHMDTCVLYDQTAFLEKWPGIPVGNEEGELIAGALGDKKAIL
CCCCCHHHEEEEECCEEECCCCEEEEECCHHHHHHCCCCCCCCCCCCEEEECCCCCEEEE
LSHHGLLVAGASVEEACVLGVMFERAARLQLLAMAAGEIQPIPPELGREAQRWVSTQKRF
EECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
EATFAYYSRRARRQLDAQ
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ANTMQLSKSEIVASSLEKMQTELRADRMPLREAVAETCRILFTFGHDSVLSGQITARAE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCEECCEEEEECC
QPGTYYTQRLGLGFDEITADNLLLVNEDLEVISGNGMPNPANRFHSWLYRARPDLNCIVH
CCCHHHHHHHCCCHHHHCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEE
SHAPHASALAMLEVPLTISHMDTCVLYDQTAFLEKWPGIPVGNEEGELIAGALGDKKAIL
CCCCCHHHEEEEECCEEECCCCEEEEECCHHHHHHCCCCCCCCCCCCEEEECCCCCEEEE
LSHHGLLVAGASVEEACVLGVMFERAARLQLLAMAAGEIQPIPPELGREAQRWVSTQKRF
EECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
EATFAYYSRRARRQLDAQ
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]